Isolation of culturable mycobiota from agricultural soils and determination of tolerance to glyphosate of nontoxigenic Aspergillus section Flavi strains

Glyphosate-based herbicides are extensively used in Argentina's agricultural system to control undesirable weeds. This study was conducted to evaluate the culturable mycobiota [colony forming units (CFU) g^?1 and frequency of fungal genera or species] from an agricultural field exposed to pesticides. In addition, we evaluated the tolerance of A. oryzae and nontoxigenic A. flavus strains to high concentrations (100 to 500 mM – 17,000 to 84,500 ppm) of a glyphosate commercial formulation. The analysis of the mycobiota showed that the frequency of the main fungal genera varied according to the analyzed sampling period. Aspergillus spp. or Aspergillus section Flavi strains were isolated from 20 to 100% of the soil samples. Sterilia spp. were also observed throughout the sampling (50 to 100%). Aspergillus section Flavi tolerance assays showed that all of the tested strains were able to develop at the highest glyphosate concentration tested regardless of the water availability conditions. In general, significant reductions in growth rates were observed with increasing concentrations of the herbicide. However, a complete inhibition of fungal growth was not observed with the concentrations assayed. This study contributes to the knowledge of culturable mycobiota from agricultural soils exposed to pesticides and provides evidence on the effective growth ability of A. oryzae and nontoxigenic A. flavus strains exposed to high glyphosate concentrations in vitro.     

Water hyacinths as a resource in agriculture and energy production: a literature review

Water hyacinths are becoming a problem in lakes, ponds and waterways in many parts of the world. This paper contains a literature study of different ways to use water hyacinths, mainly in agricultural or alternative energy systems. The literature review indicated that water hyacinths can be rich in nitrogen, up to 3.2% of DM and have a C/N ratio around 15. The water hyacinth can be used as a substrate for compost or biogas production. The sludge from the biogas process contains almost all of the nutrients of the substrate and can be used as a fertiliser. The use of water hyacinth compost on different crops has resulted in improved yields. The high protein content makes the water hyacinth possible to use as fodder for cows, goats, sheep and chickens. Water hyacinth, due to its abundant growth and high concentrations of nutrients, has a great potential as fertiliser for the nutrient deficient soils of Africa and as feed for livestock. Applying the water hyacinths directly without any other processing than sun drying, seems to be the best alternative in small-scale use due to the relatively small losses of nutrients and workload required. To meet the ever-growing energy demand, biogas production could be one option but it requires investments and technological skills that would impose great problems in developing countries where the water hyacinth is often found. Composting as an alternative treatment has the advantage of a product that is easy to work into the soil compared with dried water hyacinths, because of the decomposed structure. Harvesting and transport of water hyacinths can be conducted manually on a small scale and does not require a new harvesting technique to be introduced. Transporting of fresh water hyacinths means, if used as fertiliser in amounts large enough to enhance or effect crop growth, an unreasonably large labour requirement. Based on the labour need and the limited access to technology, using dried water hyacinths, as green manure is a feasible alternative in many developing countries.     

Herbicides based on 2,4-D: its behavior in agricultural environments and microbial biodegradation aspects. A review

Environmental Science and Pollution Research - One of the main herbicides used in the agricultural environments is 2,4-dichlorophenoxyacetic acid (2,4-D). It is a synthetic plant hormone auxin...     

Evaluation of genotoxicity and toxicity of water and sediment samples from a Brazilian stream influenced by tannery industries

This paper reports results of genotoxicity and toxicity studies of water and sediment samples collected from the Estancia Velha stream of southern Brazil, a stream transporting both domestic sewage and effluents from regional factories working in the leather industry. Three sites were selected: in the stream headwaters (Site 1), located downstream of an urban area (Site 2), and near the basin outfall (Site 3). Results obtained with Allium cepa showed no evidence of chromosomal mutation, either in water or in sediment, during winter or summer seasons, but samples collected below Site 1 showed high toxicity. Physical and chemical analyses showed high concentrations of pollutants at these sites. Ecotoxicity tests with Daphnia magna and Ceriodaphnia dubia measured toxicity in water from Sites 2 and 3 in summer 2004. A toxic effect on Hyalella azteca was only found in sediment from Site 3 during winter 2003 and summer 2004. The results suggest that the synergy among different compounds in domestic and industrial sewage discharges can make it difficult to maintain system stability.     

Effects of chronic exposure to lead,copper, zinc,and cadmium on biomarkers of the European eel,Anguilla anguilla

Exposure to specific metallic compounds can cause severe deleterious modifications in organisms. Fishes are particularly prone to toxic effects from exposure to metallic compounds via their environment. Species that inhabit estuaries or freshwater environments can be chronically affected by persistent exposure to a large number of metallic compounds, particularly those released by industrial activities. In this study, we exposed yellow eels (European eel, Anguilla anguilla) for 28 days to environmentally relevant concentrations of four specific metals; lead (300, 600, and 1,200 μg/l), copper (40, 120, and 360 μg/l), zinc (30, 60, and 120 μg/l) and cadmium (50, 150, and 450 μg/l). The selected endpoints to assess the toxicological effects were neurotransmission (cholinesterasic activity in nervous tissue), antioxidant defense, and phase II metabolism (glutathione-S-transferase [GST] activity, in both gills and liver tissues), and peroxidative damage. The results showed an overall lack of effects on acetylcholinesterase for all tested metals. Lead, copper, and cadmium exposure caused a significant, dose-dependent, increase in GST activity in gill tissue. However, liver GST only significantly increased following zinc exposure. No statistically significant effects were observed for the thiobarbituric acid reactive substances assay, indicating the absence of peroxidative damage. These findings suggest that, despite the occurrence of an oxidative-based response after exposure to lead, copper, and cadmium, this had no consequence in terms of peroxidative membrane damage; furthermore, cholinergic neurotoxicity caused by lead, copper, and cadmium did not occur. The implications of these results are further discussed.     

Bisphenol A migration from plastic materials: direct insight of ecotoxicity in Daphnia magna

Bisphenol A (BPA) is an endocrine disrupting chemical (EDC) whose migration from food packaging is recognized worldwide. However, the real overall food contamination and related consequences are yet largely unknown. Among humans, children’s exposure to BPA has been emphasized because of the immaturity of their biological systems. The main aim of this study was to assess the reproductive impact of BPA leached from commercially available plastic containers used or related to child nutrition, performing ecotoxicological tests using the biomonitoring species Daphnia magna. Acute and chronic tests, as well as single and multigenerational tests were done. Migration of BPA from several baby bottles and other plastic containers evaluated by GC-MS indicated that a broader range of foodstuff may be contaminated when packed in plastics. Ecotoxicological test results performed using defined concentrations of BPA were in agreement with literature, although a precocious maturity of daphnids was detected at 3.0 mg/L. Curiously, an increased reproductive output (neonates per female) was observed when daphnids were bred in the polycarbonate (PC) containers (145.1?±?4.3 % to 264.7?±?3.8 %), both in single as in multigenerational tests, in comparison with the negative control group (100.3?±?1.6 %). A strong correlated dose-dependent ecotoxicological effect was observed, providing evidence that BPA leached from plastic food packaging materials act as functional estrogen in vivo at very low concentrations. In contrast, neonate production by daphnids cultured in polypropylene and non-PC bottles was slightly but not significantly enhanced (92.5?±?2.0 % to 118.8?±?1.8 %). Multigenerational tests also revealed magnification of the adverse effects, not only on fecundity but also on mortality, which represents a worrying trend for organisms that are chronically exposed to xenoestrogens for many generations. Two plausible explanations for the observed results could be given: a non-monotonic dose–response relationship or a mixture toxicity effect.     

Recent Trends and Patterns of Nutrient Concentrations in Small Agricultural Streams in Sweden

Long-term median total phosphorus (TotP) and total nitrogen (TotN) concentrations were 0.08 and 5.5 P/N (mg L^-1), respectively, in 10 agricultural streams monitored since 1988 in Sweden. The areas ofthe respective catchments are 2–20 km². The period 1992/2002 was characterised by stable hydrological conditions without any flow trends in nearly all of the streams. The highest average TotP concentration, 0.17 mg P L^-1, was found in a small agriculturalstream in the largest Swedish agricultural plain. The soil texture is here characterised by a large specific surface area of the soil particles, and the agriculture by cereal production. The second highestaverage TotP concentration, 0.14 mg P L ^-1, was measured in the surface water from a catchment characterised partly by clay soils andby production of potato, spring cereals and grass. This catchment had twice as many fields with a calculated high risk for P losses comparedwith another monitored catchment in the same watershed (River Rönneå). There was a significant downward TotP trend during 1992/2002 of 0.0012 mg P L^-1 yr^-1 (Sen's slope estimator) in the catchment where many fields risk P losses and which had a reduced P manure application rate of –20% during 1995/2000. In recent years practically no manure has been spread during autumn. Bypass flow of nitrate through one soil has been suggested to influence the LOWESS (LOcally WEighted Scatterplot Smothing) fitting curve of TotN. Total nitrogen concentration decreased in most of the catchment. The average downward slope was similar to a general TotN reduction of 0.069 mg N L^-1 yr^-1. During the period 1992/2002 this was equal to slightly more than 10 per cent. Cultivation of catch crops wasrelatively uncommon until 2002, but this practice is expected to expandto larger areas during 2003 and in the future.     

The role of participation in the planning process: examples from Sweden

Participation in decision-making has successively developed into a guiding principle at both EU and national level. However, diverse perspectives exist on what the role of different interests in participative processes should be, and the legal rules regarding participation varies between different sectors; from clearly defined to virtually non-existent requirements. This may have adverse effects on the legitimacy of decisions and decision-making. This paper reviews the role of participation in the planning process in relation to natural resource development in Sweden, as guided by EU and international law. Based on the notion of effective participation, the study illustrates the potential clashes that may result from different conceptions of participation, for instance, at various levels of governance, as well as from disparate principles for implementation in different sectors.     

Wind energy potential analysis for Thailand: Uncertainty from wind maps and sensitivity to turbine technology

Rapid development of wind energy has been witnessed in Thailand. However, different wind resource maps (over land) have brought great uncertainty to wind energy planning. Here, four important mesoscale wind maps were considered: DEDP (2001), World Bank (2001), Manomaiphiboon et al. (2010) of JGSEE, and DEDE (2010). The wind maps were first harmonized to a common grid at 100 m and then compared. The earlier wind maps (DEDP and World Bank) are shown to represent the lower and upper limits of predicted speed, respectively, while JGSEE and DEDE tend to be more moderate with predictions statistically closer to observations. A consolidated wind map was constructed based on their median and shown to have the best prediction performance. It was then used for the technical potential analysis, in which three large (2-MW) turbine models (two conventional and one designed for low wind speed) were considered. By GIS techniques, any land areas not feasible for large wind turbines were excluded, and the corresponding overall onshore technical potential ranges between 50 and 250 GW, depending on map and turbine model. Considering only economically feasible turbines (with capacity factors of 20%) and the median-based map, the final technical potential equals 17 GW when using the low-wind-speed model but is reduced to 5 GW with the conventional models, adequately meeting the national wind energy target of 3 GW by the year 2036. The results suggest a strong sensitivity of estimated technical potential to turbine technology and a suitability of low-wind-speed turbines for wind conditions in Thailand.