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81.
Chandra A 《Journal of environmental biology / Academy of Environmental Biology, India》2011,32(3):347-354
In general tropical forage legumes lack microsatellites or simple sequence repeat (SSR) markers. Development of genic SSR markers from expressed sequence tagged (EST) database is an alternate and efficient approach to generate the standard DNA markers for genome analysis of such crop species. In the present paper a total of 816 EST-SSRs containing perfect repeats of mono (33.5%), di (14.7%), tri (39.3%), tetra (2.7%), penta (0.7%) and hexa (0.4%) nucleotides were identified from 1,87,763 ESTs of Medicago truncatula. Along with, 70 (8.5%) SSRs of a compound type were also observed. Seven primer pairs of tri repeats were tested for cross transferability in 19 accessions of forage legumes comprising 11 genera. At two different annealing temperatures (55 and 60 degreesC) all primer pairs except AJ410087 reacted with many accessions of forage legumes. Atotal of 51 alleles were detected with six M. truncatula EST-SSRs primer-pairs against DNAfrom 19 accessions representing 11 genera where number of alleles ranged from 2 to 13. The cross-transferability of these EST-SSRs was 40.6% at 55 degreesC and 32.3% at 60 degreesC annealing temperature. 24 alleles of the total 50 (48%) at 55 degreesC and 27 of 51 (53%) at 60 degreesC were polymorphic among the accessions. These 27 polymorphic amplicons identified could be used as DNA markers. This study demonstrates the developed SSR markers from M. truncatula ESTs as a valuable genetic markers and also proposes the possibility of transferring these markers between species of different genera of the legumes of forage importance. It was evident from the results obtained with a set of Desmanthus virgatus accessions where SequentialAgglomerative Hierarchical and Nested (SAHN) cluster analysis based on Dice similarity and Unweighted Pair Group Method with Arithmetic mean Algorithm (UPGMA) revealed significant variability (24 to 74%) among the accessions. High bootstrap values (>30) supported the nodes generated by dendrogram analysis of accessions. 相似文献
82.
Ningombam Linthoingambi Devi Ishwar Chandra Yadav Q. I. Shihua Surendra Singh S. L. Belagali 《Environmental monitoring and assessment》2011,177(1-4):23-33
Pulp and paper mills generate varieties of pollutants depending upon type of the pulping process being used. This paper presents the characteristics of wastewater from South India Paper Mill, Karnataka, India which is using recycled waste paper as a raw material. The raw wastewater consists of 80?C90 mg L???1 suspended solid and 1,010?C1,015 mg L???1 dissolved solid. However, pH varied from 5.5?C6.8. The biochemical oxygen demand and chemical oxygen demand ranged from 200?C210 and 1,120?C1,160 mg L???1, respectively. Aerobic treatment of raw effluent attribute to significant reduction in suspended solid (range between 25 to 30 mg L???1) and total dissolved solid (range between 360 to 390 mg L???1). However, pH, temperature, and electrical conductivity were found superior after treatment. Copper, cadmium, iron, lead, nickel, and zinc were found in less quantity in raw effluent and were almost completely removed after treatment. The dendrogram of the effluent quality parameters clearly indicate that South India Paper Mill does not meet Minimal National Standard set by central Pollution Control Board to discharge in agricultural field. 相似文献
83.
Das Basanta Kumar Roy Aparna Som Sukanya Chandra Ganesh Kumari Suman Sarkar Uttam Kumar Bhattacharjya Birendra Kumar Das Archan Kanti Pandit Arun 《Environmental science and pollution research international》2022,29(6):8452-8463
Environmental Science and Pollution Research - The COVID-19 pandemic has created unprecedented human health crisis in recent global history with rippling social and economic effects. The outbreak... 相似文献
84.
Kulithalai Shiyam Sundar Parthasarathy Deka Paresh Chandra 《Environmental science and pollution research international》2022,29(57):86220-86236
Environmental Science and Pollution Research - Land use and land cover (LULC) change has become a critical issue for decision planners and conservationists due to inappropriate growth and its... 相似文献
85.
Roy Kumarjyoti Sikdar Debdas Pongwisuthiruchte Aphiwat Debnath Subhas Chandra Potiyaraj Pranut 《Journal of Material Cycles and Waste Management》2022,24(4):1533-1541
Journal of Material Cycles and Waste Management - The application of waste materials based fillers is unique way to achieve rubber composites, which reduces the amount of waste in the environment... 相似文献
86.
Biswas Manik Chandra Jony Bodiuzzaman Nandy Pranab Kumar Chowdhury Reaz Ahmed Halder Sudipta Kumar Deepak Ramakrishna Seeram Hassan Masud Ahsan Md Ariful Hoque Md Enamul Imam Muhammad Ali 《Journal of Polymers and the Environment》2022,30(1):51-74
Journal of Polymers and the Environment - Recently, the advantages of biopolymers over conventional plastic polymers are unprecedented, provided that they are used in situations in which they raise... 相似文献
87.
A method for the solid phase extraction of trace metals, namely Co, Cu, Pb, Ni and Zn, from environmental and biological samples using column Amberlite XAD-7 loaded with 2-hydroxy-propiophenone-4-phenyl-3-thiosemicarbazone (HPPPTSC) and determination by inductively coupled spectrometry (ICP–AES) has been developed. The reagent has the capacity to form chelate complexes with the metals because of three binding sites in the reagent molecule. The optimum experimental conditions for the quantitative sorption of five metals, pH, effect of flow rate, concentration of eluent, sorption capacity and the effect of diverse ions on the preconcentration of analytes have been investigated. The sorption capacity of the resin has 83, 127, 35, 88 and 85?µmol?g?1 for Co2+, Cu2+, Pb2+, Ni2+ and Zn2+, respectively. The preconcentration factors for Co2+, Cu2+, Pb2+, Ni2+ and Zn2+ were 100, 110, 120, 140 and 150, respectively. The accuracy of the proposed procedure was evaluated by standard reference materials. The achieved results were in good agreement with certified values. The proposed method was applied for the determination of trace metals in river water and plant leaves. 相似文献
88.
89.
Krishna Giri J. P. N. Rai Shailesh Pandey Gaurav Mishra Rajesh Kumar Deep Chandra Suyal 《Chemistry and Ecology》2017,33(9):817-825
Isoproturon (IPU)-degrading soil bacteria were isolated from herbicide-applied wheat fields. These isolates were identified using cultural, morphological, biochemical and 16S rRNA sequencing methods. 16S rRNA sequences of both the bacterial isolates were compared with NCBI GenBank data base and identified as Bacillus pumilus and Pseudoxanthomonas sp. A soil microcosm study was carried out for 40 days in six different treatments. Experimental results revealed maximum 95.98% IPU degradation in treatment 6 where bacterial consortia were augmented in natural soil, followed by 91.53% in treatment 5 enriched with organic manure as an additional carbon source. However, only 14.03% IPU was degraded in treatment 1 (control) after 40 days. In treatments (2–4), 75.59%, 70.92% and 77.32% IPU degradation was recorded, respectively. IPU degradation in all the treatments varied significantly over the control. 4-Isopropylaniline was detected as IPU degradation by-product in the medium. The study confirmed that B. pumilus and Pseudoxanthomonas sp. performed effectively in soil microcosms and could be employed profitably for field-scale bioremediation experiments. 相似文献
90.
Shyam Lal Sethuraman Venkataramani Manish Naja Jagdish Chandra Kuniyal Tuhin Kumar Mandal Pradip Kumar Bhuyan Kandikonda Maharaj Kumari Sachchida Nand Tripathi Ujjaini Sarkar Trupti Das Yerramsetti Venkata Swamy Kotalo Rama Gopal Harish Gadhavi Modathi Kottungal Satheesh Kumar 《Environmental science and pollution research international》2017,24(26):20972-20981
Surface ozone is mainly produced by photochemical reactions involving various anthropogenic pollutants, whose emissions are increasing rapidly in India due to fast-growing anthropogenic activities. This study estimates the losses of wheat and rice crop yields using surface ozone observations from a group of 17 sites, for the first time, covering different parts of India. We used the mean ozone for 7 h during the day (M7) and accumulated ozone over a threshold of 40 ppbv (AOT40) metrics for the calculation of crop losses for the northern, eastern, western and southern regions of India. Our estimates show the highest annual loss of wheat (about 9 million ton) in the northern India, one of the most polluted regions in India, and that of rice (about 2.6 million ton) in the eastern region. The total all India annual loss of 4.0–14.2 million ton (4.2–15.0%) for wheat and 0.3–6.7 million ton (0.3–6.3%) for rice are estimated. The results show lower crop loss for rice than that of wheat mainly due to lower surface ozone levels during the cropping season after the Indian summer monsoon. These estimates based on a network of observation sites show lower losses than earlier estimates based on limited observations and much lower losses compared to global model estimates. However, these losses are slightly higher compared to a regional model estimate. Further, the results show large differences in the loss rates of both the two crops using the M7 and AOT40 metrics. This study also confirms that AOT40 cannot be fit with a linear relation over the Indian region and suggests for the need of new metrics that are based on factors suitable for this region. 相似文献