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291.
Lavinia L. Ruta Claudia V. Popa Ioana Nicolau Ileana C. Farcasanu 《Environmental science and pollution research international》2016,23(24):24514-24526
To respond to metal surpluses, cells have developed intricate ways of defense against the excessive metallic ions. To understand the ways in which cells sense the presence of toxic concentration in the environment, the role of Ca2+ in mediating the cell response to high Cu2+ was investigated in Saccharomyces cerevisiae cells. It was found that the cell exposure to high Cu2+ was accompanied by elevations in cytosolic Ca2+ with patterns that were influenced not only by Cu2+ concentration but also by the oxidative state of the cell. When Ca2+ channel deletion mutants were used, it was revealed that the main contributor to the cytosolic Ca2+ pool under Cu2+ stress was the vacuolar Ca2+ channel, Yvc1, also activated by the Cch1-mediated Ca2+ influx. Using yeast mutants defective in the Cu2+ transport across the plasma membrane, it was found that the Cu2+-dependent Ca2+ elevation could correlate not only with the accumulated metal, but also with the overall oxidative status. Moreover, it was revealed that Cu2+ and H2O2 acted in synergy to induce Ca2+-mediated responses to external stress. 相似文献
292.
Milena Petriccione Stefania Papa Claudia Ciniglia 《Environmental science and pollution research international》2014,21(5):3491-3502
Walnut husk washing waters (WHWW), a by-product of walnut production, are indiscriminately used for irrigation without preliminary risk assessment. Basing on previous in vitro results on the toxicity of this by-product, we have followed the morphophysiological development of Zea mays, Lactuca sativa cv. Gentilina and L. sativa cv. Canasta under diluted and undiluted WHWW irrigation. Significant development alterations have been observed in root and shoot elongations for all crops as well as in total biomass and chlorophyll content. The genotoxic potential of WHWW has been concurrently verified; acridine orange/ethidium bromide staining evidenced chromatin modifications and DNA degradation and also was confirmed by DNA laddering. The DNA instability was also assessed through RAPD, thus suggesting the danger of the by-product of walnut processing and focusing the attention on the necessity of an efficient treatment of WHWWs. The findings obtained by PCA of agronomic and physiological traits suggested that establishing guidelines for the administration of WHWW for irrigation is of great importance, and it is necessary to supervise their use in agricultural soils. 相似文献
293.
Silvia Berkner Claudia Thierbach 《Environmental science and pollution research international》2014,21(16):9461-9467
Human pharmaceutical active ingredients that are orally or parenterally administered may be metabolised in the body and after excretion may be further transformed in the receiving environmental compartments. The optimal outcome from an environmental point of view—complete mineralisation—is rarely observed. Small molecule pharmaceuticals are commonly not readily biodegradable according to Organisation for Economic Cooperation and Development (OECD) 301 tests. However, primary transformation is often observed. To gain information on the transformation of active ingredients in the environment, long-term studies like transformation in aquatic water/sediment systems according to OECD guideline 308 are required for the environmental risk assessment for human active pharmaceutical ingredients. Studies received until mid 2010 as part of the dossiers for marketing authorisation applications were evaluated concerning transformation products. The evaluation revealed that in 70 % of the studies, at least one transformation product (TP) is formed above 10 % of the originally applied dose, but in only 26 % of the studies are all TP identified. The evaluation also revealed that some TP of pharmaceutical active ingredients show a considerably longer DT50 compared to the parent compound. An example is the TP (val)sartan acid that is formed from an antihypertensive compound. 相似文献
294.
295.
Ulrike Hirt Judith Mahnkopf Mathias Gadegast Lukas Czudowski Ute Mischke Claudia Heidecke Gerald Schernewski Markus Venohr 《Regional Environmental Change》2014,14(3):1123-1138
We introduce an approach for establishing reference conditions (RC) for rivers of the German Baltic Sea catchment, based on predictive modelling. An extensive data set of statistics from the year 1880 was coupled with literature data, providing a comprehensive basis for the calculation of regional historical nutrient emissions into rivers, river nutrient concentrations, and nutrient loading into the sea. Four different scenarios were calculated: scenario 1 assumed RC following previously established criteria for lakes (Poikane et al. in Environ Manag 45(6):1286–1298, 2010), scenario 4 applied the nutrient emission conditions of 1880 (considering non-intensive land usage, the presence of tile drainage systems, sewer systems, and the human population from 1880), and scenarios 2 and 3 considered intermediate conditions. Our results showed that nutrient emissions from scenario 1 accounted for approximately one-tenth of the currently observed total nitrogen (TN) and total phosphorus (TP) emissions. The nutrient emissions calculated for 1880 (scenario 4) were found to be 47 % (TN) and 55 % (TP) higher than those calculated considering reference lake conditions (scenario 1). Our results suggest that RC nutrient concentrations in rivers in the German Baltic Sea catchment were clearly below 0.05 mg/l for TP and 1 mg/l for TN. This range is in accordance with historical and calculated pristine nutrient concentrations from other rivers in similar catchments. 相似文献
296.
The island of Navarino, Chile, located at the extreme southern end of the Americas, is one of the few regions in the world with undivided and only slightly transformed temperate forests. Currently, fundamental issues are being addressed, such as how local fuel wood demands will be met without destroying primary forests and how a sustainable tourism industry may be developed. This study aims to inform these planning processes by providing data on the economic valuation of several non-market benefits provided by the temperate ecosystems of Navarino Island that have relevance to the local population. We focus this valuation on landscape esthetics, nature access restrictions, esthetic and ethno-symbolic benefits at the species level and the existence value of non-vascular endemic species. A choice experiment was applied to a sample of local residents (n = 230). Decisions about future development strategies were influenced by landscape esthetics being threatened by progressing levels of tourist infrastructure, nature access restrictions in favor of both economic and conservationist concerns, continued visits of an ethno-culturally important hummingbird, the protection of a moss endemic to the sub-Antarctic forests and species diversity. From a non-market valuation perspective, local residents favor a low-impact tourism development scenario. Little is known about the monetary value of Chile’s temperate forests. Knowledge of the economic value of Navarino’s temperate forests facilitates the understanding of local natural resource management at the microlevel and assists in formulating conservation policies at the regional and national levels. 相似文献
297.
Milena Petriccione Valentina Forte Diego Valente Claudia Ciniglia 《Environmental science and pollution research international》2013,20(7):4859-4871
Catechol is a highly toxic organic pollutant, usually abundant in the waste effluents of industrial processes and agricultural activities. The environmental sources of catechol include pesticides, wood preservatives, tanning lotion, cosmetic creams, dyes, and synthetic intermediates. Genotoxicity of catechol at a concentration range 5?×?10?1–5 mM was evaluated by applying random amplified polymorphic DNA (RAPD) and time-lapse DNA laddering tests using onion (Allium cepa) root cells as the assay system. RAPD analysis revealed polymorphisms in the nucleotidic sequence of DNA that reflected the genotoxic potential of catechol to provoke point mutations, or deletions, or chromosomal rearrangements. Time-lapse DNA laddering test provided evidence that catechol provoked DNA necrosis and apoptosis. Acridine orange/ethidium bromide staining could distinguish apoptotic from necrotic cells in root cells of A. cepa. 相似文献
298.
Kristin B. Byrd Elliott Matchett Claudia Mengelt Tamara S. Wilson Deanne DiPietro Monica Moritsch Erin Conlisk Sam Veloz Michael L. Casazza Matthew E. Reiter 《Conservation biology》2023,37(5):e14089
Scientists, resource managers, and decision makers increasingly use knowledge coproduction to guide the stewardship of future landscapes under climate change. This process was applied in the California Central Valley (USA) to solve complex conservation problems, where managed wetlands and croplands are flooded between fall and spring to support some of the largest concentrations of shorebirds and waterfowl in the world. We coproduced scenario narratives, spatially explicit flooded waterbird habitat models, data products, and new knowledge about climate adaptation potential. We documented our coproduction process, and using the coproduced models, we determined when and where management actions make a difference and when climate overrides these actions. The outcomes of this process provide lessons learned on how to cocreate usable information and how to increase climate adaptive capacity in a highly managed landscape. Actions to restore wetlands and prioritize their water supply created habitat outcomes resilient to climate change impacts particularly in March, when habitat was most limited; land protection combined with management can increase the ecosystem's resilience to climate change; and uptake and use of this information was influenced by the roles of different stakeholders, rapidly changing water policies, discrepancies in decision-making time frames, and immediate crises of extreme drought. Although a broad stakeholder group contributed knowledge to scenario narratives and model development, to coproduce usable information, data products were tailored to a small set of decision contexts, leading to fewer stakeholder participants over time. A boundary organization convened stakeholders across a large landscape, and early adopters helped build legitimacy. Yet, broadscale use of climate adaptation knowledge depends on state and local policies, engagement with decision makers that have legislative and budgetary authority, and the capacity to fit data products to specific decision needs. 相似文献
299.
Ziková A Trubiroha A Wiegand C Wuertz S Rennert B Pflugmacher S Kopp R Mareš J Spurný P Kloas W 《Journal of environmental monitoring : JEM》2010,12(12):2276-2281
Nile tilapia (Oreochromis niloticus) were fed by diets supplemented with cyanobacteria containing in part the cyanotoxin microcystin-LR (MC-LR) to determine the potential impacts on detoxification. Four different diets were prepared based on a commercial diet: (1) control, (2) MC-5% (containing 5% dried Microcystis sp. biomass with 4.92 μg MC-LR g(-1) diet), (3) MC-20% (containing 20% dried Microcystis sp. biomass with 19.54 μg MC-LR g(-1) diet), and (4) Arthr-20% (containing 20% dried Arthrospira sp. biomass without MC-LR). Blood and liver samples were taken after one, 7, and 28 days and protein has been determined in plasma and liver. In the liver, impacts on detoxification were measured by glutathione-S-transferase (GST) activities and gene expression of multi drug resistance protein (MDRP). Plasma protein did not change between all four diets at any sampling time whereas liver protein was significantly elevated already after one day in Arthr-20% and after 28 days in both, MC-20% and Arthr-20%. Biochemical measurements of GST activities revealed no significant impact at any sampling time. In order to characterize the potential effect of MC-LR on MDRP, RT-qPCR method was established. However, as for GST activities no significant changes in MDRP gene expression have been observed. Thus, in summary, oral exposure of MC-LR containing cyanobacteria to Nile tilapia via feed ingestion did not impact significantly detoxification in liver concerning GST activities and MDRP expression despite biochemical composition concerning liver protein was significantly elevated by the diets containing 20% cyanobacteria biomass, regardless whether they contained MC-LR or not. 相似文献
300.
Ciniglia C Sansone C Panzella L Napolitano A d'Ischia M 《Environmental science and pollution research international》2012,19(8):3299-3306
INTRODUCTION: The reuse of wastewaters for agricultural purposes is a common practice in many countries and is increasingly recommended by organizations that promote sustainable development. Yet, it is restricted by the potential negative impact of these materials on soil and crops. The aim of this study was therefore to evaluate the environmental impact of walnut husk washing waters (WHWW) and their organic fractions, in order to conceive their agricultural exploitation. DISCUSSION: Phytotoxicity tests and morphological investigations on representative plant species of horticultural interest indicated that WHWW and their organic fractions can elicit a concentration-dependent stimulating effect on the growth of radish, lettuce cv. cavolo Napoli with effects up to 165 %. An opposite inhibitory effect up to 70 % was observed on spinach and lettuce cv. Gentilina. Proapoptotic effects were observed by acridine orange/ethidium bromide assay in the species inhibited by WHWW treatment. High-performance liquid chromatography-mass spectrometry analysis of the WHWW revealed the presence of a main component which was extracted selectively in organic solvents and purified by preparative chromatography. Complete spectral analysis allowed identification as 4,8-dihydroxy-1-tetralone, commonly known as regiolone. Regiolone exhibited the same concentration-dependent activity on root elongation with a stimulation in the case of radish up to 135 % with respect to control. These results open perspectives in the exploitation of WHWW and the main phenolic constituent readily available by a straightforward isolation procedure as a natural fertilizer for specific crops. 相似文献