Mutagenic and carcinogenic potency of extracts of diesel and related environmental emissions: Study design, sample generation, collection, and preparation

A major diesel emissions research program has been initiated by the U.S. Environmental Protection Agency to assess the human health risk associated with increased use of diesel automobiles. This program is intended to establish the mutagenic and carcinogenic potency of complex organics associated with diesel particles as well as comparative particle-bound organics from other environmental emissions for which human epidemiological data are available. The mobile source samples selected for this study were collected from a heavy-duty diesel engine, a series of light-duty diesel passenger cars, and a gasoline catalyst automobile. The comparative source samples incorporated into the study were cigarette smoke condensate, coke oven emissions, roofing tar emissions, and benzo(a)pyrene. The samples were tested using three mutagenic assays and four carcinogenic assays as prescribed by a test matrix. This report describes the study design, particle generation, and sample collection and preparation. A brief summary of the bioassays is also included.     

Mutagenic and carcinogenic potency of extracts from diesel related environmental emissions: Simultaneous morphological transformation and mutagenesis in BALB/c 3t3 cells

Particulate extracts from six different environmental emission sources were assayed for genotoxic activity in mouse BALB/c 3T3 clone A31-1 cells. All compounds were tested simultaneously for both transforming and mutagenic (induction of ouabain-resistance) potential with and without exogenous metabolic activation in the form of a 9000 × g postmitochondrial hepatic supernatant fraction from Aroclor-1254 induced Fischer 344 rats. Dichloromethane particulate extracts from the exhaust of two light duty diesel engines (Oldsmobile and Nissan), one heavy duty diesel engine (Caterpillar) and one late model gasoline engine (Mustang II) were assayed in an identical manner to particulate extracts from the emissions of a roofing tar pot and a coke oven. No clear dose-dependent responses were observed, but several of the samples showed significant transforming and mutagenic activity. A qualitative ranking system showed the activity of these particulate extracts for either mutagenesis or transformation was: coke oven = Mustang II gasoline engine > Nissan diesel engine > roofing tar. Particulate extracts from the Oldsmobile diesel engine and the Caterpillar diesel engine showed essentially no activity.     

Plutonium in sheep faeces as an indicator of deposition on vegetation

In order to study the deposition on vegetation of plutonium arising from the testing of nuclear weapons and discharges from nuclear facilities, sheep faeces were collected from the South-west and North of England. The plutonium content of faeces has been shown to be a reproducible and sensitive indicator of deposition on herbage and, with this technique, plutonium discharged from the Sellafield Works of British Nuclear Fuels LImited can be detected 60 km from the site. On moorland pastures the concentration of ^239+240Pu in faeces, expressed as activity per gram of ash, is roughly equal numerically to the deposit of plutonium on 1 m² of vegetation.Some of the plutonium ingested by a grazing sheep is absorbed and the highest concentrations are found in liver. The liver of a sheep grazing on pasture where the ^239+240Pu concentration in faeces was about 2 pCi (74 mBq) g⁻¹ of ash, was found to contain <1% of the Generalised Derived Limit for mutton and offal, and the meat <0·001%.     

Health effects of oxidants

Oxidants of significance to human health include ozone, nitrogen dioxide, and peroxyacetylnitrate. All of these compounds are involved in complex photochemical reactions which makes quantification and prediction of their individual health effects difficult. Ozone causes trauma to lung tissues and interferes with enzyme systems in the lungs and other tissues causing a broad range of symptoms. Measurable health impacts can occur at concentrations as low as 390 μg/m³. Acute effects of ozone exposure are reversible at normal urban concentrations (80–120 μg/m³). A special problem of concern, however, is increased susceptibility to infectious diseases contracted through the lungs. Nitrogen dioxide also causes trauma to lung tissues and interferes with enzyme systems. Measurable impacts can occur at concentrations as low as 100 μg/m³, but recovery is rapid and it is not known whether repeated exposures at this level have cumulative effects or predispose the lungs to permanent damage. Chronic exposure of laboratory animals to higher nitrogen dioxide levels can cause emphysema-like conditions and reduction in resistance to respiratory infection. Epidemiological studies of children in houses with gas stoves confirm the finding of reduced resistance to respiratory infection. The U.S. EPA estimates that health effects may occur in young children exposed to concentrations in excess of 280 to 560 μg/m³ one-hour average. These concentrations occur routinely in houses having gas stoves. Peroxyacetylnitrate is a powerful eye irritant in photochemical smog. Other health effects are similar to those of ozone, but less important because of the relatively low concentrations of this pollutant compared to other oxidants.     

An appraisal of emissions control requirements in the California South Coast Air Basin

The purpose of this study is to evaluate the effect of reductions of reactive organic gases (ROG) and NO_x emissions on short-term O₃ and NO₂ concentrations and annual average NO₂ concentrations in the California South Coast Air Basin. Short-term air quality predictions were obtained by applying the Systems Applications Airshed Model to summer O₃ and autumn NO₂ episodes. Effects of emission controls on annual NO₂ concentrations were estimated using CDM and a new parcel tracking model NOXTRAK. Results for the summer O₃ episode indicate that ROG emission reduction in an effective means for reducing peak O₃ concentrations. NO_x emission reduction imposed in addition to ROG emission reductions are counterproductive in reducing peak O₃ concentrations. The modeling results also suggest that attainment of the 1-h federal O₃ standard requires ROG emission reductions on the order of 80% from 1987 levels. Results for the autumn NO₂ episode indicate that NO_x emission reductions approximating those recommended in a proposed Air Quality Management Plan (about 22%) will result in only small (about 5%) reductions in the peak NO₂ concentrations. ROG emission reduction may be more effective than NO_x emission reduction in reducing the peak NO₂ concentration. For the episode studied, a reduction of 36% in ROG emissions is estimated to result in a reduction in peak NO₂ concentrations commensurate with that required to attain the 1-h state NO₂ standard. Model calculations also indicate that the federal NO₂ standard may not be meet by 1987 at one or two stations, but may blosely approached.     

Effect of rise in spring air temperature on the arrival dates and reproductive success of the Song Thrush, <Emphasis Type="Italic">Turdus philomelos</Emphasis> (C.L. Brehm, 1831) in the forest–steppe of the Russian Plain

The effect of rise in spring air temperature on the breeding phenology and reproductive success of the Song Thrush in the Voronezh Nature Reserve have been studied over the periods of 1987 to 1990 and 2008 to 2012. The results show that relatively high air temperatures provide for earlier arrival of the birds from wintering grounds, earlier and more synchronous breeding of the majority of nesting pairs, and an increase in clutch size and proportion of pairs producing two broods per season. Predation pressure on bird nests decreases under such conditions, which markedly improves reproductive success. Climate warming is conducive to increasing breeding productivity of the Song Thrush population as a whole.     

Carbon fluxes from coarse woody debris in southern taiga forests of the Valdai Upland

Studies in three typical forest biotopes of the Valdai Upland were performed to evaluate the stocks and surface area of coarse woody debris from spruce and birch (in linear transects), its colonization by xylotrophic fungi (during reconnaissance surveys), and CO₂ emission (by a chamber method). The stock and surface area were minimum in a paludal birch forest (46.4 m³/ha and 960 m²/ha) and maximum in a decay area of spruce forest (256.1 m³/ha and 3761 m²/ha, respectively). The assemblages of wood-decay fungi had a composition typically found in southern taiga forests. The total CO₂ flux varied from 145 kg C-CO₂/ha per year in the paludal birch forest to 462 kg C-CO₂/ha per year in small herb–green moss spruce forest. It is concluded that air temperature is an informative predictor of seasonal C-CO₂ flux rate from coarse woody debris.     

Impact of urban anthropogenic pollution on seed production,morphological and biochemical characteristics of chamomile,Matricaria chamomila L.

It has been shown that the growth of technogenic dust pollution is accompanied by the accumulation of heavy metals in soil and plant phytomass, compared to the control, and that the morphometric and reproductive characteristics of Matricaria chamomila are most sensitive to the impact of these pollutants. The response of plants is manifested in increased variation in morphological characters, mobilization of compensatory protective biochemical mechanisms, and production of more viable seed progeny.