Repetitive extragenic palindromic PCR (REP-PCR) as a method used for bulking process detection in activated sludge

Bulking of activated sludge is a world-widely prevalent problem and can lead to loss of bio-oxidation, further deterioration of effluent quality, and even to a complete breakdown of the entire treatment process. Most common reasons of bulking are bacterial community changes, especially excessive growth of filamentous bacteria or excess of biopolymers on surface of non-filamentous microbes. Because of complex nature of the bulking phenomenon, the successful bulking control strategy finding is still a very important need awaiting new options and advices. The repetitive extragenic palindromic PCR (REP-PCR) fingerprinting method has been applied to distinguish bacterial community in non-bulking and bulking activated sludge. The characteristic REP-PCR fingerprinting patterns, using the Ward??s clustering method, have been analyzed to determine homology/similarity relation between particular non-bulking and bulking sludge sampling. The received clustering results were in high concordance with activated sludge typing done based on physicochemical sludge analysis. The choice and application of molecular typing method in sludge analysis will depend upon the needs, skill level, and resources of the laboratory. The proposed REP-PCR method and statistical analysis of fingerprinting patterns seems to be simple, rapid, and effective methods to show differences between population in non-bulking and bulking activated sludge. It is easy to implement, and it may be useful for routinely activated sludge monitoring as well as may be helpful in early detection of bulking process.     

Box Handling in the Loading and Unloading of Vans

The handling of 2,306 boxes being loaded or unloaded from vans onto or from 4-wheeled trolleys by 31 handlers in a warehouse were characterized. Handling was videotaped and characterized through an analysis grid completed by three trained observers. The following execution parameters were observed: nature of the exertion applied by the upper limbs, plane and direction of the exertion, resulting displacement of the box, grip, use of the lower limbs and the back. Results show that execution parameters used by handlers vary considerably from those usually recommended or studied. For example, symmetric grips were rarely used (4%). The grip was modified during the handling of half the boxes. Significant knee flexion was rarely observed (3% of exertions). Each box was moved by applying an average of . 3.5 different exertions. Exertions were mostly applied in a plane parallel to the shoulders; they were rarely executed in a strict sagittal plane (11%). The implication of these observations are discussed.     

Cultivating collaboration: Lessons from initiatives to understand and manage cumulative impacts in Australian resource regions

Australia has experienced rapid development within its resource regions, with traditional mining sectors like coal, iron-ore and natural gas expanding and new industries such as coal seam gas emerging. As a result, there is an increasing prevalence and awareness of the cumulative impacts of the extractive resource industries on the society, environment and economy of these regions. Collaborative governance is emerging as a means of addressing cumulative impacts. This article undertakes an analysis of 30 case studies of collaborative governance in the resources sector of Australia. The initiatives analysed range from those focussed on information exchange and coordination to higher degrees of collaboration that involve shared resources and shared risks. The study demonstrates that there are challenges in using collaborative approaches to tackle cumulative impacts, but that significant benefits can be realised. The study highlights the need to nurture and cultivate collaborative relationships in order to provide the foundation for long-term solutions.     

Assessing Natural and Anthropogenic Variability in Wetland Structure for Two Hydrogeomorphic Riverine Wetland Subclasses

The hydrogeomorphic approach (HGM) to wetland classification and functional assessment has been applied regionally throughout the United States, but the ability of HGM functional assessment models to reflect wetland condition has limited verification. Our objective was to determine how variability derived from anthropogenic effects and natural variability impacted site assessment variables within regional wetland subclasses in central Oklahoma. We collected data for nine potential assessment variables including vegetation physiognomy (e.g., tree basal area, herbaceous cover, canopy cover, etc.) and soil organic matter at wetlands of two HGM riverine subclasses (oxbow and riparian) in May and June, 2010. Using Akaike Information Criteria, we identified limited relationships between landscape disturbance metrics and assessment variables within subclasses. The high degree of natural variability from climatic and hydrologic factors within both subclasses may be masking the impact of landscape disturbance on the other measured assessment variables. Precipitation had significant effects on assessment variables within each of the subclasses. To reduce natural climatic variability, the reference domain may need to be further subdivided. The approach used in this study provides fairly rapid and quantitative methods for evaluating the effectiveness of using HGM assessment variables in assessing wetland condition regionally.     

Managing Forests for Increased Regional Water Yield in the Southeastern U.S. Coastal Plain

With growing populations fueling increased groundwater abstraction and forecasts of greater water scarcity in the southeastern United States, identifying land management strategies that enhance water availability will be vital to maintaining hydrologic resources and protecting natural systems. Management of forested uplands for lower basal area, currently a priority for habitat improvement on public lands, may also increase water yield through decreased evapotranspiration (ET). To explore this hypothesis, we synthesized studies of precipitation and ET in coastal plain pine stands to develop a statistical model of water yield as a function of management strategy, stand structure, and ecosystem water use. This model allowed us to estimate changes in water yield in response to varying management strategies across spatial scales from the individual stand to a regional watershed. Results suggest that slash pine stands managed at lower basal areas can have up to 64% more cumulative water yield over a 25‐year rotation compared to systems managed for high‐density timber production, with the greatest increases in stands also managed for recurrent understory fire. Although there are important uncertainties in the magnitude of additional water yield and its final destination (i.e., surface water bodies vs. groundwater), this analysis highlights the potential for management activities on public and private timber lands to partially offset increasing demand on surface and groundwater resources.     

The Integrated Pest Management Educator Pilot Project in Boston Public Housing: Results and Recommendations

Abstract

The German cockroach is the most common pest of urban, low-income housing in the United States and is associated with high pesticide use by residents. Cockroach allergen is implicated in asthma exacerbation and initiation and in the growing social and medical aspects of the disease. A safe and secure home environment is an environmental justice issue, and environmentally sound and effective models of cockroach control are needed in public and low-income multifamily housing. One model that offers potential is the integrated pest management (IPM) peer educator model (IPM Educator) in public housing as a component of IPM. A pre–post-analysis of an IPM Educator pilot programme in Boston public housing was undertaken. Thirty-four moderate- to high-infested units received baseline assessment and three applications of gel baits and IPM treatment by a pest control operator. Before the first pest control application, residents received written notice to prepare their units for treatment, a standard procedure. Before the second and third pest control treatments, an IPM Educator instructed residents in basic pest biology and habits, preparation for treatment, and the role of sanitation in pest control. Results showed a significant improvement in rate of preparation for pest control treatment after the IPM Educator intervention when compared with the preparation rate before the education intervention, and a significant reduction in cockroach populations by the third visit in units that were prepared and had improved sanitation, when compared with unprepared units and unclean units. The IPM peer educator is a promising, low-cost model of educating and engaging residents of urban, low-income, multifamily housing in environmentally sound pest control, thereby giving them some power and control over their living environment. It is also a potential source of professional training and jobs for public housing and other low-income housing residents.     

Community Sustainability Plans to enable change towards sustainable practice – a Scottish case study

There is a need for a global shift towards a low-carbon society and this requires action at the local level. The aim of the study into sustainable community development is to clarify the role of a community as a whole rather than the role of individuals within that community. Mixed methods of literature review and empirical study have been used to translate the knowledge and experience with urban sustainable development into empirical knowledge of a rural, market town setting. The exemplary case study is the Scottish market town Huntly. The main findings of the study show the need for three ingredients for a Community Sustainability Plan for a town such as Huntly. Community involvement is crucial in developing such plans in a rural setting. Further research in more remote settings, e.g. coastal and island communities, is needed to allow broader conclusions on rural sustainable development.