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71.
Anna Doffini Claudio Forcato Chiara Mangano Debora Lattuada Roberta Aversa Chiara Maranta Emilia D. Giovannone Genny Buson Chiara Bolognesi Rebecca Maiocchi Martina Dori Liyana Jamal Raidah B. Ahmad George S. H. Yeo Tai Wai Yeo Silvia Saragozza Rosamaria Silipigni Marta Serafini Andrea Biondi Sofia Perego Patrizia Vergani Enrico Ferrazzi Paola Ricciardi-Castagnoli Thomas J. Musci Francesca Romana Grati 《黑龙江环境通报》2023,43(1):14-27
Objective
To develop a multi-step workflow for the isolation of circulating extravillous trophoblasts (cEVTs) by describing the key steps enabling a semi-automated process, including a proprietary algorithm for fetal cell origin genetic confirmation and copy number variant (CNV) detection.Methods
Determination of the limit of detection (LoD) for submicroscopic CNV was performed by serial experiments with genomic DNA and single cells from Coriell cell line biobank with known imbalances of different sizes. A pregnancy population of 372 women was prospectively enrolled and blindly analyzed to evaluate the current workflow.Results
An LoD of 800 Kb was demonstrated with Coriell cell lines. This level of resolution was confirmed in the clinical cohort with the identification of a pathogenic CNV of 800 Kb, also detected by chromosomal microarray. The mean number of recovered cEVTs was 3.5 cells per sample with a significant reverse linear trend between gestational age and cEVT recovery rate and number of recovered cEVTs. In twin pregnanices, evaluation of zygosity, fetal sex and copy number profiling was performed in each individual cell.Conclusion
Our semi-automated methodology for the isolation and single-cell analysis of cEVTS supports the feasibility of a cell-based noninvasive prenatal test for fetal genomic profiling. 相似文献72.
Pisa Lennard Goulson Dave Yang En-Cheng Gibbons David Sánchez-Bayo Francisco Mitchell Edward Aebi Alexandre van der Sluijs Jeroen MacQuarrie Chris J. K. Giorio Chiara Long Elizabeth Yim McField Melanie Bijleveld van Lexmond Maarten Bonmatin Jean-Marc 《Environmental science and pollution research international》2021,28(10):11749-11797
Environmental Science and Pollution Research - New information on the lethal and sublethal effects of neonicotinoids and fipronil on organisms is presented in this review, complementing the... 相似文献
73.
Marcon Alessandro Panunzi Silvia Stafoggia Massimo Badaloni Chiara de Hoogh Kees Guarda Linda Locatelli Francesca Silocchi Caterina Ricci Paolo Marchetti Pierpaolo 《Environmental science and pollution research international》2021,28(22):28096-28106
Environmental Science and Pollution Research - Chipboard production is a source of ambient air pollution. We assessed the spatial variability of outdoor pollutants and residential exposure of... 相似文献
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Mate guarding is a male strategy to gain access to receptive females but often results in antagonistic interactions between
the sexes because of different costs/benefits of guarding. In addition to social, morphological, and physiological parameters,
the type of mating system should also affect the strength of the conflict and thus the guarding duration. Specifically, when
compared to females, self-compatible hermaphrodites might have reduced benefits of outcrossing. We investigated mate guarding
in dioecious (co-presence of females and males) and androdioecious (co-presence of hermaphrodites and males) branchiopod crustaceans.
Both sexes in androdioecious systems should shift their guarding times to lower values relative to dioecious systems because
(1) androdioecious males are present in lower percentages than dioecious males and thus encounter rates with receptive mates
are relatively greater for them; and (2) hermaphrodites should have low incentive to incur high costs of mate guarding, having
the alternative of self-fertilization, and thus should be highly eager to resist. While females preferred short guarding times,
when allowed to control the guarding duration (males tethered), dioecious males did not increase their guarding duration when
females (treated with muscular relaxant) could not resist, in contrast to what has previously been found for androdioecious
males. This indicates that hermaphrodites are more willing to resist mate guarding than females. The among-species comparisons
supported our hypotheses: compromised guarding times were significantly lower in androdioecious than in dioecious species.
The introduction of a parameter (mating system) not previously investigated in mate guarding models resulted in a powerful
test of mate guarding theory, adding a valuable contribution to our understanding of intersexual conflict. 相似文献
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Chiara Lombardi Silvia Cocito Anna Occhipinti-Ambrogi Keith Hiscock 《Marine Biology》2006,149(5):1103-1109
Zooid size variation and growth banding in the bryozoan Pentapora fascialis, collected from the South of Great Britain to the South of Italy, were investigated in order to test their relationship with the variation of seawater temperature. Zooid length appeared to be more sensitive than zooid area and width to temperature variation. The longest zooids were budded in localities that typically experience low seasonal variation in temperature (3.2–7.5°C) and low mean annual temperature (about 11°C). Zooid length demonstrated the strongest relationship with the range of seasonal variation in temperature rather than the mean annual temperature. Growth bands produced during colder months were less high than those produced during warmer months. Measurements of these alternating bands were used to calculate growth rate of the laminae, ranging from 20.7 at Plymouth to 29.3 mm year−1 at Tino Island. Significant differences in zooid length were found between the two bands, with longer zooids within the band developed in colder months and shorter zooids in bands produced in the warmer period. We conclude that zooid size can be used as an environmental proxy of the seasonal temperature regimes experienced by the species. 相似文献