Technical and theoretical considerations in the HLA typing of amniotic fluid cells for prenatal diagnosis and paternity testing

HLA typing of amniotic fluid cells has been used for the prenatal diagnosis of the HLA linked diseases congenital adrenal hyperplasia (21-OH-deficiency (21-OH-def) type) and complement C4 deficiency and it has also been used for the prenatal de termination of paternity. There are, however, technical difficulties in this test associated with the weak expression of some B locus antigens on amniotic fluid cells, and theoretical difficulties related to associations between particular HLA antigens and the 21-OH-def allele. Since certain HLA-B locus antigens are found in significantly increased frequencies among patients with 21-OH-def, there is a relatively high incidence of HLA-B homozygosity among the patients and over 40 percent of the parents of these patients share one or more HLA-B locus antigens. Results of some prenatal HLA typing tests may thus be difficult to interpret, and supplementary tests should be used whenever possible. HLA typing of amniotic cells is, however, the only available procedure for prenatal diagnosis of C4 deficiency and it is the best available procedure for prenatal determination of paternity. A modification of our original procedure allows HLA typing to be performed with increased numbers of HLA typing sera, and sera with optimum reactivity for amniotic fluid cells have now been selected for the definition of most of the more commonly expressed HLA antigens. Although amniotic fluid cells do not express DR antigens, amniotic fluid cells can be typed for the HLA-linked marker glyoxalase I (GLO) and this may be the informative for prenatal diagnosis in some cases.     

论环境质量管理在线监控指挥系统建设

本文着重阐述在原有的“污染源在线监控系统”的基础上，通过架构更高层次的“环境质量管理在线监控指挥系统”，在环境监察行政执法上突破原有的“运动式”执法和宏观表面管理，实现在环境监察行政执法上的“点对点”数字化精确管理与在线指挥，在环境质量管理上突破原有的人工行政审批制，实现“以人为本”的环境质量管理电子政务行政许可制，以及由此带来环境质量管理理念、管理机制和环境监察行政执法工作模式的创新。提高行政效能，服务经济建设。     

油田行业"三废"综合利用研究

介绍了国家“三废”综合利用方面的政策，结合油田行业资源综合利用现状，提出如何进一步开展油田行业“三废”综合利用工作，并更好地利用国家资源利用税收方面的优惠政策，开拓油田行业“三废”综合利用渠道，推动“三废”综合利用，争取得到最大限度的减免税额。     

Effects of bifenthrin on Daphnia magna during chronic toxicity test and the recovery test

IntroductionWiththedevelopmentandapplicationofnewpesticide ,insecticidehasgreatlyreducedtheharmofpesttocrops .Bifenthrinisaneffectivepyrethroidinsecticideandacaricideagainstawiderangeofinsectpests ,anditisverypoisonoustomammal,aquatic .Thisinsecticideiswi…