Methane oxidation and abundance of methane oxidizers in tropical agricultural soil (vertisol) in response to CuO and ZnO nanoparticles contamination

There is worldwide concern over the increase use of nanoparticles (NPs) and their ecotoxicological effect. It is not known if the annual production of tons of industrial nanoparticles (NPs) has the potential to impact terrestrial microbial communities, which are so necessary for ecosystem functioning. Here, we have examined the consequences of adding the NPs particularly the metal oxide (CuO, ZnO) on CH₄ oxidation activity in vertisol and the abundance of heterotrophs, methane oxidizers, and ammonium oxidizers. Soil samples collected from the agricultural field located at Madhya Pradesh, India, were incubated with either CuO and ZnO NPs or ionic heavy metals (CuCl₂, ZnCl₂) separately at 0, 10, and 20 μg g^?1 soil. CH₄ oxidation activity in the soil samples was estimated at 60 and 100 % moisture holding capacity (MHC) in order to link soil moisture regime with impact of NPs. NPs amended to soil were highly toxic for the microbial-mediated CH₄ oxidation, compared with the ionic form. The trend of inhibition was Zn 20?>?Zn 10?>?Cu 20?>?Cu 10. NPs delayed the lag phase of CH₄ oxidation to a maximum of 4-fold and also decreased the apparent rate constant k up to 50 % over control. ANOVA and Pearson correlation analysis (α?=?0.01) revealed significant impact of NPs on the CH₄ oxidation activity and microbial abundance (p?<?0.0001, and high F statistics). Principal component analysis (PCA) revealed that PC1 (metal concentration) rendered 76.06 % of the total variance, while 18.17 % of variance accounted by second component (MHC). Biplot indicated negative impact of NPs on CH₄ oxidation and microbial abundance. Our result also confirmed that higher soil moisture regime alleviates toxicity of NPs and opens new avenues of research to manage ecotoxicity and environmental hazard of NPs.     

Assessment of the effect of the toxicity of a textile dye on Nostoc muscorum ISU, a diazotrophic cyanobacterium

The impact of graded concentrations (5, 10, 15 and 20 mg dm(-3) of acid mordant Metomega Chrome Orange GL, a common dye used in woollen, carpet and textile industries, was studied on protein and pigment content and photosynthetic oxygen evolution in a diazotrophic cyanobacterium Nostoc muscorum. The lower concentration of 5 mg dm(-3) showed a negligible effect on protein and pigment content and photosynthetic oxygen evolution, whereas at higher concentrations a drastic decrease in the above parameters was observed. The highest concentration of 20 mg dm(-3) resulted in a decrease of protein, chlorophyll a, phycocyanin and carotenoid content by 72, 76, 54 and 17% of the control, respectively. Photosynthetic oxygen evolution also decreased by 92% of the control at this concentration of the dye.     

Removal of chromate ions from leachate-contaminated groundwater samples of Khan Chandpur,India, using chitin modified iron-enriched hydroxyapatite nanocomposite

Environmental Science and Pollution Research - Chromite ore processing residues (COPR) are real environmental threats, leading to CrO42-, i.e., Cr (VI) leaching into groundwater. It is of serious...     

Growth, yield and photosynthesis of Panicum maximum and Stylosanthes hamata under elevated CO2

Plant height, biomass production, assimilatory functions and chlorophyll accumulation of Panicum maximum and Stylosanthes hamata in intercropping systems was influenced significantly under elevated CO2 (600 +/- 50 ppm) in open top chambers (OTCs). The plant height increased by 32.0 and 49.0% over the control in P. maximum and S. hamata respectively in intercropping system under elevated CO2 over open field grown crops (Ca). P. maximum and S. hamata produced 67 and 85% higher fresh and dry biomass respectively under elevated CO2. Rates of photosynthesis and stomatal conductance increased in both the crop species in intercropping systems under elevated CO2. The canopy photosynthesis (photosynthesis x leaf area index) of these crop species increased significantly under elevated CO2 over the open grown crops. The chlorophyll a and b accumulation were also higher in the leaves of both the crop species as grown in OTC with elevated CO2. The increased chlorophyll content, leaf area index and canopy photosynthesis led to higher growth and biomass production in these crop species under elevated CO2. The total carbon sequestration in crop biomass and soils during the three years was 21.53 Mg C/ha under elevated CO2. The data revealed that P. maximum and S. hamata intercropping system is the potential as a sink for the increasing level of CO2 in the atmosphere in the semi-arid tropics.     

Acute toxicity evaluation of triazophos,deltamethrin and their combination on earthworm,Eudrilus eugeniae and its impact on AChE activity

The acute toxicity of three formula grade pesticides namely, triazophos (an organophosphate, OP), deltamethrin (a pyrethroid) and combined pesticide (triazophos?+?deltamethrin) was determined in earthworm, Eudrilus eugeniae. They were exposed to different concentrations of these pesticides for 48 h by paper contact toxicity method. The LC₅₀ values for triazophos, deltamethrin and of combination were determined as 0.076, 0.031 and 0.065?μg/cm², respectively. To study the sublethal effect of these pesticides, E. eugeniae were exposed to 5% and 10% of LC₅₀ of pesticides for 48 h. Morphological alterations such as coiling, clitellar swelling, mucus release and bleeding followed by body segmentation were observed in exposed earthworm. Acetylcholinesterase (AChE) activity assayed in different regions of body segment exhibited a significant (p?<?0.05) decrease in its activity particularly in the pre-clitellar region as compared to other regions. The altered behavioural responses in pesticides exposed earthworms would have been due to decline in AChE activity of the nervous system.     

Rural energy transitions in developing countries: a case of the Uttam Urja initiative in India

In most developing countries, at the household level, traditional burning of biomass or use of inefficient technologies for domestic applications like lighting is common, triggering concerns related to fuel or technology switching. The paper focuses on opportunities to promote cleaner energy options through development of value chains delivering improved energy efficiency and access in developing countries. We discuss the example of Uttam Urja, a field project involving the dissemination of photovoltaic lighting technologies in rural areas of India. We focus on the challenges of introducing radical innovations into the residential energy sector in developing countries. For the purpose of this paper the Uttam Urja project is conceptualized as an ‘experiment’ and analysed using the Strategic Niche Management (SNM) framework. The paper emphasizes that to effect socio-technical transitions to clean energy options on the ground, it is desirable to focus on technology customization and innovative financing to cater to the needs and concerns of end users.     

Influence of the wastewater composition on denitrification and biological P-removal in the S-DN-P process： （c） Dissolved and undissolved substrates

The denitrification and P-removal in the sorption-denitrification-P-removal (S-DN-P) process were carried out under various wastewater compositions. It is noted that P-removal largely depends on the wastewater composition as well to the quantity of the substrates present in wastewater fraction. Three different wastewater fractions are obtained as: raw wastewater, dissolved wastewater (obtained with filtration using 0.45 μm filter), and undissolved wastewater (i.e., infiltrate obtained by above filtration). ...     

Study on concentration of ambient NH 3 and interactions with some other ambient trace gases

We present diurnal variation of ambient ammonia (NH₃) in relation with other trace gases (O₃, CO, NO, NO₂, and SO₂) and meteorological parameters at an urban site of Delhi during winter period. For the first time, ambient ammonia (NH₃) was monitored very precisely and continuously using ammonia analyzer, which operates on chemiluminescence method. NH₃ estimation efficiency of the chemiluminescence method (>90%) is much higher than the conventional chemical trapping method (reproducibility 4.5%). Ambient NH₃ concentration reaches its maxima (46.17 ppb) at night and minimum during midday. Result reveals that the ambient ammonia (NH₃) concentration is positively correlated with ambient NO (r ²?=?0.79) and NO₂ (r ²?=?0.91) mixing ratio and negatively correlated with ambient temperature (r ²?=???0.32). Wind direction and wind speed indicates that the nearby (~500 m NW) agricultural fields may be major source of ambient NH₃ at the observational site.