The lichen-rich coastal heath vegetation on the isle of Anholt, Denmark — description, history and development

The marine foreland called Ørkenen (=the desert) at Anholt is covered by lichen-richEmpetrum nigrum-Corynephorus canescens heath vegetation on beach ridg e systems formed during the elevation of land after the Weichsel ice age andStereocaulon saxatile-richAmmophila arenaria-Corynephorus canescens vegetation on semi-fixed aeolic dunes. The development of the vegetation at Ørkenen is the result of various kinds of human exploitation, with its maximum in the 16th and 17th century. After the cessation of human use, a slow succession towards closed vegetation with mainlyEmpetrum nigrum andCorynephorus canescens began. The present heath ecosystem, developed on sandy substrate with very little organic matter and a small nutrient supply, is a high priority habitat in Europe.     

Spatial and temporal variation of metal concentrations in adult honeybees (Apis mellifera L.)

Honeybees (Apis mellifera L.) have great potential for detecting and monitoring environmental pollution, given their wide-ranging foraging behaviour. Previous studies have demonstrated that concentrations of metals in adult honeybees were significantly higher at polluted than at control locations. These studies focused at a limited range of heavy metals and highly contrasting locations, and sampling was rarely repeated over a prolonged period. In our study, the potential of honeybees to detect and monitor metal pollution was further explored by measuring the concentration in adult honeybees of a wide range of trace metals, nine of which were not studied before, at three locations in the Netherlands over a 3-month period. The specific objective of the study was to assess the spatial and temporal variation in concentration in adult honeybees of Al, As, Cd, Co, Cr, Cu, Li, Mn, Mo, Ni, Pb, Sb, Se, Sn, Sr, Ti, V and Zn. In the period of July-September 2006, replicated samples were taken at 2-week intervals from commercial-type bee hives. The metal concentration in micrograms per gram honeybee was determined by inductive coupled plasma-atomic emission spectrometry. Significant differences in concentration between sampling dates per location were found for Al, Cd, Co, Cr, Cu, Mn Sr, Ti and V, and significant differences in average concentration between locations were found for Co, Sr and V. The results indicate that honeybees can serve to detect temporal and spatial patterns in environmental metal concentrations, even at relatively low levels of pollution.     

Microscale investigations of the fate of heavy metals associated to iron-bearing particles in a highly polluted stream

As it flows through a dense steelmaking area, the Fensch River does transport iron-rich particles and colloids, displaying high contents in metallic contaminants (Zn, Cr, Pb, Cu, Ni, and As). Chemical analysis using inductively coupled plasma mass spectrometry (ICP-MS) was carried out on three compartments—waters, suspended materials, and sediments—along the river linear. The variations of metallic trace element concentrations along the river were shown to be partially related to external inputs (industrial and domestic wastewaters and urban surfaces leaching). However, some discrepancies of element partitioning were evidenced. Pb, Cu, and Mn tend to concentrate in suspended particulate and in dissolved fraction, while Cr and As follow the trend of Fe and concentrate within sediments of the most downstream station, just before the junction with Moselle waters. Zn appears strongly associated to iron-rich particles, resulting in a decrease of its concentration in waters for the last station. Along the Fensch linear, the variation of metal partitioning between water and particulate phases is accompanied with strong modifications of the nature and mineralogy of iron-rich particles, as evidenced by microanalyses using electron and X-ray beams. The combination of bulk analyses using ICP-MS and microanalyses applied to the three compartments allowed us to propose a three-step process “settling–weathering–resuspension” to explain Zn partitioning.     

In vivo genotoxicity assessment of titanium,zirconium and aluminium nanoparticles,and their microparticulated forms,in Drosophila

As in vivo system, we propose Drosophila melanogaster as a useful model for study the genotoxic risks associated with nanoparticle exposure. In this study we have carried out a genotoxic evaluation of titanium dioxide (TiO₂), zirconium oxide (ZrO₂) and aluminium oxide (Al₂O₃) nanoparticles and their microparticulated forms in D. melanogaster by using the wing somatic mutation and recombination assay. This assay is based on the principle that loss of heterozygosis and the corresponding expression of the suitable recessive markers, multiple wing hairs and flare-3, can lead to the formation of mutant clones in treated larvae, which are expressed as mutant spots on the wings of adult flies. Third instar larvae were feed with TiO₂, ZrO₂ and Al₂O₃ nanoparticles, and their microparticulated forms, at concentrations ranging from 0.1 to 10 mM. Although a certain level of aggregation/agglomeration was observed in solution, it must be noted than the constant digging activity of larvae ensures that treated medium pass constantly through the digestive tract ensuring exposure. The results showed that no significant increases in the frequency of all spots (e.g. small single, large single, twin, total mwh and total spots) were observed, indicating that these nanoparticles were not able to induce genotoxic activity in the wing spot assay of D. melanogaster. Negative data were also obtained with the microparticulated forms. This indicates that the nanoparticulated form of the selected nanomaterials does not modify the potential genotoxicity of their microparticulated versions. These in vivo results contribute to increase the genotoxicity database on the TiO₂, ZrO₂ and Al₂O₃ nanoparticles.     

Health and Welfare in Danish Dairy Cattle in the Transition to Organic Production: Problems, Priorities and Perspectives

During the past few years,organic dairy farming has grown dramatically inDenmark. Consequently, an increasing number ofpeople are encountering this method ofproduction for the first time. Amongst these,many veterinarians have suddenly had to dealwith organic herds in their home district, and,meeting examples of poor animal welfare, theyhave recently started to express some concerns.Against this background, a so-called``Synthesis of Knowledge' project was initiatedto examine the health and welfare of dairycattle and the use of medication in thetransition to organic production. The aim ofthe project was to investigate associatedproblems from the point of view of the dairyherd. Based on qualitative research interviewswith vets and agricultural advisors, as well asfocus group interviews with farmers who hadrecently converted from conventional to organicfarming, an expert panel attempted to identifyproblem areas and possible solutions. Theproblems related to (a) the adjustment to newand unknown practices, (b) poor management ingeneral, and (c) inappropriate legislation fororganic farming.One problem area was the rearing of dairycalves, particularly with regard to theestablishment of post partumrelationships between cow and calf, grouphousing, and the grazing of young animals. A``natural life' was identified as beingfundamental to organic animal husbandry. Interms of animal welfare, this concept can beunderstood as a way of living in which the calfis allowed to express its natural behavior andsatisfy its natural needs. Essentially, theconditions needed for good calf welfare requirethe compatibility and interplay of elementsfrom ``nature' (the natural life; includingopportunities to satisfy natural needs) and``culture' (farmer management skills; exerciseof care). These elements should be balanced toensure animal welfare in organic herds.     

Low VOC latex paints from a precipitation polymerization process

Even though paints and coatings have been very useful in protecting various substrates, their use has been accompanied by a heavy environmental price. There is a need to minimize the use of volatile organic compounds (VOCs) in the formulation of these materials. In the latex paint area, we have implemented a conventional precipitation process in emulsions to form binder particles that contain block copolymers and have softer blocks on the particle surface. The softer surface provides the binder film-forming properties without the need for the use of solvents or VOCs. The covalent connections between the soft blocks and the hard blocks are expected to impart good performance in the resulting dry coatings. Indeed, we have shown that our test coating from these binder particles containing block copolymer have similar properties in most performance categories to those of a semi-gloss control paint that has been formulated with typical amounts of VOCs.     

The effects of proximal and distal goals on performance on a moderately complex task

The effect of a proximal plus a distal goal was investigated relative to setting only a distal goal or urging participants to do their best. Young adults (N=39) were paid on a piece rate basis to make toys. An analysis of variance revealed that the amount of money earned by the participants who were urged to ‘do your best’ was significantly greater than the amount of money earned by the participants who were assigned a distal goal. However, the amount of money earned by the participants who were assigned proximal goals, in addition to a distal goal, was significantly greater than the amount of money earned by the participants in the ‘do your best’ condition. The correlation between perceived self‐efficacy and the amount of money earned was 0.45 (p<0.01). Perceived self‐efficacy significantly increased only for those participants in the proximal plus distal goal condition. Proximal goals, through self‐efficacy and performance feedback, appear to have focused attention on task appropriate strategies. The results suggest an informational explanation of proximal goals as opposed to a motivational one through goal commitment. Implications of these findings for mentoring and training are discussed. Copyright © 1999 John Wiley & Sons, Ltd.     

Assessing PAH and PCB emissions from the relocation of harbour sediments using equilibrium passive samplers

Large-scale dredging of contaminated sediments is taking place in the harbor of Oslo, Norway. The dredged sediment masses are transferred into a confined aquatic disposal facility (CAD) in a natural 70-m deep basin within the Oslofjord. Currently there is no established method to determine how much dissolved contaminants are released during relocation and deposition of these sediments. For this reason we tested the use of equilibrium passive samplers consisting of 55 microm thin polyoxymethylene (POM-55) for studying the release of freely dissolved and thus bioavailable PAHs and PCBs at the disposal site, and found this to be a suitable method. In order to use POM-55 for monitoring PCBs, it was necessary to measure their POM-55/water partition coefficients, which was also presented as part of this study. Elevated turbidity (average 4.1 mg l(-1)) was observed at one side of the basin where no natural sill exists. Analysis of POM-55 at this location before and after deposition revealed that there was an increase in freely dissolved concentrations (C(W,free)) during deposition by a factor 37.5 for PAHs and a factor of 2.9 for PCBs. In addition, during deposition phenanthrene-to-anthracene aqueous concentration ratios at this location (values of 3-4) were more similar to those of the deposited sediments (approximately 2) than to those of the CAD water prior to deposition (approximately 14). This was not observed for the other locations where a natural sill exists at approximately 30 m water depth. The POM-55 equilibrium passive samplers are here shown to be useful tools for measuring and understanding the dynamics involved in the release of dissolved contaminants during sediment relocation.     

The law and economics of reclamation bonds

It is becoming a standard practice for governments to require mining operations to post reclamation bonds. Yet, there have been few theoretical treatments examining the rationale for bonding mechanisms, and even fewer empirical treatments of the effectiveness of bonding. This paper addresses some of these holes in the literature. It begins by examining the rationale underlying reclamation bonds, and discusses the strengths and weaknesses of bonding as a tool for enforcing reclamation requirements. The role of bonding mechanisms is to help enforce standards, not necessarily yield efficient outcomes, and these mechanisms are best viewed as a complement to — not a substitute for — liability rules. The paper also examines the effectiveness of bonding by drawing on evidence from hardrock mining on public lands in the western United States.     

The variability of processes involved in transgene dispersal—case studies from Brassica and related genera

Background, aim, and scope

We strive to predict consequences of genetically modified plants (GMPs) being cultivated openly in the environment, as human and animal health, biodiversity, agricultural practise and farmers’ economy could be affected. Therefore, it is unfortunate that the risk assessment of GMPs is burdened by uncertainty. One of the reasons for the uncertainty is that the GMPs are interacting with the ecosystems at the release site thereby creating variability. This variability, e.g. in gene flow, makes consequence analysis difficult. The review illustrates the great uncertainty of results from gene-flow analysis.

Main features

Many independent experiments were performed on the individual processes in gene flow. The results comprise information both from laboratory, growth chambers and field trials, and they were generated using molecular or phenotypic markers and analysis of fitness parameters. Monitoring of the extent of spontaneous introgression in natural populations was also performed. Modelling was used as an additional tool to identify key parameters in gene flow.

Results

The GM plant may affect the environment directly or indirectly by dispersal of the transgene. Magnitude of the transgene dispersal will depend on the GM crop, the agricultural practise and the environment of the release site. From case-to-case these three factors provide a variability that is reflected in widely different likelihoods of transgene dispersal and fitness of introgressed plants. In the present review, this is illustrated through a bunch of examples mostly from our own research on oilseed rape, Brassica napus. In the Brassica cases, the variability affected all five main steps in the process of gene dispersal. The modelling performed suggests that in Brassica, differences in fitness among plant genome classes could be a dominant factor in the establishment and survival of introgressed populations.

Discussion

Up to now, experimental analyses have mainly focused on studying the many individual processes of gene flow. This can be criticised, as these experiments are normally carried out in widely different environments and with different genotypes, and thus providing bits and pieces difficult to assemble. Only few gene-flow studies have been performed in natural populations and over several plant generations, though this could give a more coherent and holistic view.

Conclusion

The variability inherent in the processes of gene flow in Brassica is apparent and remedies are wished for. One possibility is to expose the study species to additional experiments and monitoring, but this is costly and will likely not cover all possible scenarios. Another remedy is modelling gene flow. Modelling is a valuable tool in identifying key factors in the gene-flow process for which more knowledge is needed, and identifying parameters and processes which are relatively insensitive to change and therefore require less attention in future collections of data. But the interdependence between models and experimental data is extensive, as models depend on experimental data for their development or testing.

Recommendations

More and more transgenic varieties are being grown worldwide harbouring genes that might potentially affect the environment (e.g. drought tolerance, salt tolerance, disease tolerance, pharmaceutical genes). This calls for a thorough risk assessment. However, in Brassica, the limited and uncertain knowledge on gene flow is an obstacle to this. Modelling of gene flow should be optimised, and modelling outputs verified in targeted field studies and at the landscape level. Last but not least, it is important to remember that transgene flow in itself is not necessarily a thread, but it is the consequences of gene flow that may jeopardise the ecosystems and the agricultural production. This emphasises the importance of consequence analysis of genetically modified plants.