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191.
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193.
Mosaic trisomy 15 was prenatally diagnosed on amniotic fluid cells from two consecutive amniocenteses and was confirmed on cells from five different fetal tissues. The proportion of normal versus trisomic cells was consistently higher in the amniotic cell cultures and-with one exception-in the fetal tissues, while serial subcultures gave different results. The slightly atypical external features and internal malformations of the affected fetus as compared to the only clinical observation from the literature are not unusual enough to allow the delineation of a specific malformation pattern. 相似文献
194.
Giovanni Monni Cristina Rosatelli Angela M. Falchi Maria T. Scalas Maria Addis Liliana Maccioni Anna Di Tucci Teresa Tuveri Proffessor Antonio Cao 《黑龙江环境通报》1986,6(1):63-68
This paper reports the results of first trimester prenatal diagnosis in a twin pregnancy at risk for homozygous β°−thalassaemia (β°−39 mutant). Trophoblast samples from both twins were obtained at 10 weeks gestation with a forceps guided by ultrasound. Trophoblast DNA analysis, carried out with the oligonucleotide technique, revealed that one fetus was homozygous and the other heterozygous for the β−39 mutant. This diagnosis was confirmed at 17 weeks gestation by amniocyte DNA analysis. DNA polymorphism analysis within the α-globin gene provided useful genetic markers for twin differentiation. 相似文献
195.
The influence of management intensity on the richness, abundance and composition of land snail species was examined in 21 calcareous, nutrient-poor cattle pastures in the northwestern Jura mountains, Switzerland. Grazing intensity was positively correlated with the extent of fertilization of the pastures. Pastures without fertilizer application and with low grazing intensity harboured more snail species and more threatened snails than pastures with annual addition of manure or pastures with manure and nitrogen fertilizer and higher grazing intensity. Fewer snail individuals, open-land species and open-land individuals were found on pastures with high than on pastures with low management intensity. To preserve the threatened snail species in dry, nutrient-poor grasslands, a network of pastures should be managed without fertilization and grazing intensity should not exceed 180 LU ha−1 d (product of livestock units per hectare and grazing days). 相似文献
196.
Effect of substrate Henry's constant on biofilter performance 总被引:4,自引:0,他引:4
Zhu X Suidan MT Pruden A Yang C Alonso C Kim BJ Kim BR 《Journal of the Air & Waste Management Association (1995)》2004,54(4):409-418
Butanol, ether, toluene, and hexane, which have Henry's constants ranging from 0.0005 to 53, were used to investigate the effects of substrate solubility or availability on the removal of volatile organic compounds (VOCs) in trickle-bed biofilters. Results from this study suggest that, although removal of a VOC generally increases with a decrease in its Henry's constant, an optimal Henry's constant range for biofiltration may exist. For the treatment of VOCs with high Henry's constant values, such as hexane and toluene, the transfer of VOCs between the vapor and liquid phases or between the vapor phase and the biofilm is a rate-determining step. However, oxygen (O2) transfer may become a rate-limiting step in treating VOCs with low Henry's constants, such as butanol, especially at high organic loadings. The results demonstrated that in a gas-phase aerobic biofilter, nitrate can serve both as a growth-controlling nutrient and as an electron acceptor in a biofilm for the respiration of VOCs with low Henry's constants. Microbial communities within the biofilters were examined using denaturing gradient gel electrophoresis to provide a more complete picture of the effect of O2 limitation and denitrification on biofilter performance. 相似文献
197.
Pyrene degradation by yeasts and filamentous fungi 总被引:8,自引:0,他引:8
Romero MC Salvioli ML Cazau MC Arambarri AM 《Environmental pollution (Barking, Essex : 1987)》2002,117(1):159-163
The saprotrophic soil fungi Fusarium solani (Mart.) Sacc., Cylindrocarpon didymum (Hartig) Wollenw, Penicillium variabile Sopp. and the yeasts Rhodotorula glutinis (Fresenius) Harrison and Rhodotorula minuta (Saito) Harrison were cultured in mineral medium with pyrene. The remaining pyrene concentrations were periodically determined during 20 incubation days, using HPLC. To assess the metabolism of pyrene degradation we added 0.1 microCi of [4,5,9,10] 14C-pyrene to each fungi culture and measured the radioactivity in the volatile organic substances, extractable, aqueous phase, biomass and 14CO2 fractions. The assays demonstrated that F. solani and R. glutinis metabolized pyrene as a sole source of carbon. Differences in their activities at the beginning of the cultures disappeared by the end of the experiment, when 32 and 37% of the original pyrene concentration was detected, for the soil fungi and yeasts, respectively. Among the filamentous fungi, F. solani was highly active and oxidized pyrene; moreover, small but significant degradation rates were observed in C. didymum and P. variahile cultures. An increase in the 14CO2 evolution was observed at the 17th day with cosubstrate. R. glutinis and R. minuta cultures showed similar ability to biotransform pyrene, and that 35% of the initial concentration was consumed at the end of the assay. The same results were obtained in the experiments with or without glucose as cosubstrate. 相似文献
198.
199.
Habitat Loss and Extinction in the Hotspots of Biodiversity 总被引:34,自引:0,他引:34
200.
Pampanin DM Marangon I Volpato E Campesan G Nasci C 《Environmental pollution (Barking, Essex : 1987)》2005,136(1):103-107
In this study, a spatial and temporal survey at three sites located in the "canals" of the Venice historic centre (Italy) and at a reference site was undertaken to evaluate stress effects on mussels sampled in the Venice urban area, where raw sewage is discharged without treatment directly into the water. A battery of biomarkers (metallothionein, micronuclei, condition index and survival in air) was used to evaluate the stress condition of the animals. At the same time the alkali-labile phosphate assay (ALP) was performed in mussel' hemolymph with the aim to find an estrogenic effect biomarker in this mussel species. Biomarker results showed an impairment of the general health condition in the mussels coming from the urban area, in agreement with the chemical analysis. Significantly higher level of the ALP was found in male mussels sampled in April in the urban area, in comparison with the ones from the reference site (P<0.001). Finally, the PCA proved an easy and useful tool to summarize the obtained results, also able to classify the data to indicate a pollution gradient in the Venice urban area. 相似文献