Correlation and toxicological inference of trace elements in tissues from stranded and free-ranging bottlenose dolphins (Tursiops truncatus)

The significance of metal concentrations in marine mammals is not well understood and relating concentrations between stranded and free-ranging populations has been difficult. In order to predict liver concentrations in free-ranging dolphins, we examined concentrations of trace elements (Al, As, Ba, Be, Cd, Co, Cu, Fe, Li, Mn, Ni, Pb, Sb, Se, Sn, total Hg (THg), V, Zn) in skin and liver of stranded bottlenose dolphins (Tursiops truncatus) from the South Carolina (SC) coast and the Indian River Lagoon, Florida (FL) during 2000-2008. Significantly higher concentrations of Zn, Fe, Se, Al, Cu and THg were found in skin while liver exhibited significantly higher Cu, Fe, Mn and THg concentrations for both study sites. Mean skin concentrations of Cu and Mn were significantly higher in SC dolphins while higher concentrations of THg and V were found in FL dolphins. In addition, liver tissues in SC dolphins exhibited significantly higher As concentrations while higher Fe, Pb, Se, THg, and V levels were found in FL dolphins. Two elements (Cu and THg) showed significant age-related correlations with skin concentration while five elements (Cu, Se, THg, Zn and V) showed age-related correlations with liver concentrations. Geographic location influenced age-related accumulation of several trace elements and age-related accumulation of THg in hepatic tissue was observed for both sites to have the highest correlations (r² = 0.90SC; r² = 0.69FL). Mean THg concentration in liver was about 10 times higher in FL dolphins (330 μg g⁻¹ dw) than those samples from SC dolphins (34.3 μg g⁻¹ dw). The mean molar ratio of Hg to Se was 0.93 ± 0.32 and 1.08 ± 0.38 for SC and FL dolphins, respectively. However, the Hg:Se ratio varied with age as much lower ratios (0.2-0.4) were found in younger animals. Of the 18 measured elements, only THg was significantly correlated in skin and liver of stranded dolphins and skin of free-ranging dolphins from both sites suggesting that skin may be useful in predicting Hg concentrations in liver tissue of free-ranging dolphins. Results indicate that 33% of the stranded and 15% of the free-ranging dolphins from FL exceed the minimum 100 μg g⁻¹ wet weight (ww) (∼400 dw) Hg threshold for hepatic damage while none from SC reached this level. Hepatic concentrations of As in SC dolphins and V in FL dolphins were also highly correlated with skin concentrations which may have some regional specificity predictive value. The present study provides the first application of trace element concentrations derived from stranded bottlenose dolphins to predict liver concentrations in free-ranging populations.     

Electrical Resistance Heating of Volatile Organic Compounds in Sedimentary Rock

Electrical resistance heating (ERH) is a common method of remediation for volatile organic compounds in unconsolidated soils, both above and below the water table. In the past eight years, ERH has been used to successfully treat 10 or more contaminated sedimentary bedrock sites. Sedimentary bedrock treatment has recently expanded to greater depths and into karst limestone environments. This article describes the implementation issues for rock remediation and provides case studies of three sites remediated by ERH in Pennsylvania and Alabama. With proper design, the remediation of sedimentary bedrock can be completed as effectively as the remediation of overburden materials. © 2014 Wiley Periodicals, Inc.     

Recovery and distribution of biosolids-derived trace metals in a clay loam soil

The long-term mobility of trace metals has been cited as a potential hazard by critics of EPA 503 rule governing the land application of biosolids. The objectives of this study were to assess the accumulation of Cu, Ni, Cd, and Zn within the soil profile; the distribution of exchangeable, specifically adsorbed, organic, and oxide fractions of each metal; and mass balance of Cu, Ni, and Zn 17 yr after a single biosolids application. Biosolids were applied to 1.5- x 2.3-m confined plots of a Davidson clay loam (fine, kaolinitic, thermic Rhodic Kandiudult) in 1984 at 0, 42, 84, 126, 168, and 210 Mg ha(-1). The highest biosolids application supplied 4.5, 750, 43, and 600 kg ha(-1) of Cd, Cu, Ni, and Zn, respectively. Soils were sampled to a depth of 0.9 m and sectioned into 5-cm increments after separating the Ap horizon. Total (EPA-3050B), bioavailable (Mehlich-I), sequential extraction, and dispersible clay analyses were performed on samples from the control, 126 Mg ha(-1), and 210 Mg ha(-1) treatments. Trace metals are still concentrated in the top 0.2 m with slight enrichment down to 0.3 m. More than 85% of applied Cu, Ni, and Zn are still found in the topsoil where biosolids was incorporated and 95% or more of the applied metals were accounted for with mass balance calculations. Mehlich-I results showed a slight increase in metal concentration down to 0.35 m. Biosolids application increased the concentrations of trace metals in all the extracted fractions. The major portions of Cu, Zn, and Ni are associated with the metal-oxides fraction. Dispersible clay content and water-soluble metal contents were low and except for water-soluble Zn they were not affected by biosolids application. Results from this study showed that 17 yr after biosolids application there was negligible movement of trace metals through the soil profile and consequently there is little risk of contamination of ground water at this site.     

SPATIAL VARIABILITY IN WATER‐BALANCE MODEL PERFORMANCE IN THE CONTERMINOUS UNITED STATES1

ABSTRACT: A monthly water‐balance (WB) model was tested in 44 river basins from diverse physiographic and climatic regions across the conterminous United States (U.S.). The WB model includes the concepts of climatic water supply and climatic water demand, seasonality in climatic water supply and demand, and soil‐moisture storage. Exhaustive search techniques were employed to determine the optimal set of precipitation and temperature stations, and the optimal set of WB model parameters to use for each basin. It was found that the WB model worked best for basins with: (1) a mean elevation less than 450 meters or greater than 2000 meters, and/or (2) monthly runoff that is greater than 5 millimeters (mm) more than 80 percent of the time. In a separate analysis, a multiple linear regression (MLR) was computed using the adjusted R‐square values obtained by comparing measured and estimated monthly runoff of the original 44 river basins as the dependent variable, and combinations of various independent variables [streamflow gauge latitude, longitude, and elevation; basin area, the long‐term mean and standard deviation of annual precipitation; temperature and runoff; and low‐flow statistics (i.e., the percentage of months with monthly runoff that is less than 5 mm)]. Results from the MLR study showed that the reliability of a WB model for application in a specific region can be estimated from mean basin elevation and the percentage of months with gauged runoff less than 5 mm. The MLR equations were subsequently used to estimate adjusted R‐square values for 1,646 gauging stations across the conterminous U.S. Results of this study indicate that WB models can be used reliably to estimate monthly runoff in the eastern U.S., mountainous areas of the western U.S., and the Pacific Northwest. Applications of monthly WB models in the central U.S. can lead to uncertain estimates of runoff.     

SATURATED SOIL HYDRAULIC CONDUCTWITY IN LOBLOLLY PINE PLANTATIONS ON DRAINED SITES1

ABSTRACT: Variation of in situ measured saturated hydraulic conductivity (KS) with stand age was examined in drained and intensively managed loblolly pine (Pinus taeda L.) plantations on very poorly drained Bayboro loam soils. Stand ages studied were 1-year-old and 14-years old. No differences in Ks values were found between the stand ages. In addition, no differences in core measured soil properties were found between the stand ages, indicating that there were no differences in the pore structure of the soil matrix. There was large variation of Ks within stands and between stands within ages. The mean within stand Ks values ranged from 0.66 cm/hr to 4.85 cm/hr. The frequency of tests exhibiting pipe flow through large non-capillary voids was significantly greater in the older stands; however, the continuity of the voids in the soil, and whether or not non-Darcy type flow would occur in a saturated profile, could not be determined.     

Sorption and transformation of biocides from hydraulic fracturing in the Marcellus Shale: a review

Environmental Chemistry Letters - Biocides are applied as chemical additives in hydraulic fracturing fluids to control subsurface microbial activity. When biocides are released into the subsurface,...     

Cooling of bat hibernacula to mitigate white-nose syndrome

White-nose syndrome (WNS) is a fungal disease that has caused precipitous declines in several North American bat species, creating an urgent need for conservation. We examined how microclimates and other characteristics of hibernacula have affected bat populations following WNS-associated declines and evaluated whether cooling of warm, little-used hibernacula could benefit bats. During the period following mass mortality (2013–2020), we conducted 191 winter surveys of 25 unmanipulated hibernacula and 6 manipulated hibernacula across Pennsylvania (USA). We joined these data with additional datasets on historical (pre-WNS) bat counts and on the spatial distribution of underground sites. We used generalized linear mixed models and model selection to identify factors affecting bat populations. Winter counts of Myotis lucifugus were higher and increased over time in colder hibernacula (those with midwinter temperatures of 3–6 °C) compared with warmer (7–11 °C) hibernacula. Counts of Eptesicus fuscus, Myotis leibii, and Myotis septentrionalis were likewise higher in colder hibernacula (temperature effects = –0.73 [SE 0.15], –0.51 [0.18], and –0.97 [0.28], respectively). Populations of M. lucifugus and M. septentrionalis increased most over time in hibernacula surrounded by more nearby sites, whereas Eptesicus fuscus counts remained high where they had been high before WNS onset (pre-WNS high count effect = 0.59 [0.22]). Winter counts of M. leibii were higher in hibernacula with high vapor pressure deficits (VPDs) (particularly over 0.1 kPa) compared with sites with lower VPDs (VPD effect = 15.3 [4.6]). Counts of M. lucifugus and E. fuscus also appeared higher where VPD was higher. In contrast, Perimyotis subflavus counts increased over time in relatively warm hibernacula and were unaffected by VPD. Where we manipulated hibernacula, we achieved cooling of on average 2.1 °C. At manipulated hibernacula, counts of M. lucifugus and P. subflavus increased over time (years since manipulation effect = 0.70 [0.28] and 0.51 [0.15], respectively). Further, there were more E. fuscus where cooling was greatest (temperature difference effect = –0.46 [SE 0.11]), and there was some evidence there were more P. subflavus in hibernacula sections that remained warm after manipulation. These data show bats are responding effectively to WNS through habitat selection. In M. lucifugus, M. septentrionalis, and possibly P. subflavus, this response is ongoing, with bats increasingly aggregating at suitable hibernacula, whereas E. fuscus remain in previously favored sites. Our results suggest that cooling warm sites receiving little use by bats is a viable strategy for combating WNS.     

Nutraceuticals and mitochondrial oxidative stress: bridging the gap in the management of bronchial asthma

Environmental Science and Pollution Research - Asthma is a chronic inflammatory disease primarily characterized by inflammation and reversible bronchoconstriction. It is currently one of the...