An immunoassay for metolachlor detection in river water and soil.

An indirect enzyme-linked immunosorbent assay (EIA) for metolachlor (2-chloro-N-(2-ethyl-6-methylphenyl)-N-(2-methoxy-1-methylethyl)acetamid e) detection in river water and soil was developed using serum obtained from rabbits immunized against the acid of metalaxyl ((N-(2,6-dimethylphenyl)-N-(methoxy-acetyl)-DL-alanine methyl ester) conjugated to bovine serum albumin. The assay had a linear working range from 1 to 50 ng/ml with a mean I50 value of 13.6 ng/ml and a lower detection limit of 2.0 ng/ml. Both the mean interwell and interassay coefficients of variation were less than 4% over the range of the standard curves for samples which had been prepared in phosphate buffered saline (PBS), river water, or soil extract. Assay cross-reactivity to the following four structurally related chloro-acetanilide pesticides were: propachlor (0%), metazachlor (0%), alachlor (23%), and metalaxyl (5,000%). Mean recoveries of metolachlor in spiked (2.0 to 32.0 ng/ml range) PBS, river water, and soil extract were 102%, 103%, and 110%, respectively. Soil samples were taken over a 56-d period from field plots treated with metolachlor and analyzed by GC and EIA. The correlation coefficient for comparison of the two methods was 0.96 with the slope of the linear regression line being 0.78. Furthermore, no statistical difference (P less than 0.05) was found between the dissipation curves of metolachlor derived from GC data versus EIA data.     

Efficiency of energy delivery systems: II. Estimating energy costs of capital equipment

Procedures are developed and summarized that allow the calculation of the energy required to manufacture and install capital equipment, such as the material needed for a coalfired power plant. Three methods are available, each with certain strengths and weaknessess The first uses both literature value and national manufacturing statistics, the second uses a previously developed technique that disaggregates energy use according to how much each industry buys from all other industries, and the third technique is based on the average national relation between energy use and dollar flow. The most important differences between estimates of energy use by the different techniques relates to the inclusion or exclusion of labor and indirect expenditures rather than to differences in the data bases of the three techniques.     

RELATIONSHIPS BETWEEN LAND USES AND RAINWATER QUALITY IN A SOUTHCENTRAL PENNSYLVANIA WATERSHED1

ABSTRACT: Spatial and temporal variability in rainfall concentrations of nutrients, major ions, and herbicides was monitored at 7 locations in or near the Conodoguinet Creek watershed in south-central Pennsylvania from 1991.1993. Results were used to (1) compare precipitation quality in forested, agricultural and urban areas, and (2) assess the practicality of using volunteer citizen monitoring in such a study. As indicated in previous studies, sulfate and nitrogen concentrations in precipitation were linked to sample pH. Concentrations of major ions in precipitation appeared to relate more to regional influences rather than local influences. However, concentrations of herbicides in precipitation may have been influenced by both regional and local use which caused compounds like atrazine, deethylatrazine, propazine, simazine, metolachior, alachlor, ametryn, and prometon to be present in detectable concentrations in rainfall. Seasonality was evident in nitrogen, sulfate, pH, and herbicide data and was suggested in calcium, iron, manganese, magnesium, orthophosphate, and chloride data. Agricultural weed control activities were probably responsible for the seasonal pattern in pesticide data which peaked in May and June. Tropical storm Danielle may have caused the apparent seasonal patterns for the other nine parameters. This storm did not follow the typical west to east movement pattern and consequently produced rainfall of relative high quality. A variety of quality assurance checks indicated that trained volunteer citizen monitors were successful participants in this intensive and extensive scientific study, collecting good quality samples in a timely manner. Without this kind of volunteer help, it is extremely difficult to complete studies that require sampling in response to natural events such as rainfall.     

Physical versus chemical effects on bacterial and bromide transport as determined from on site sediment column pulse experiments

Twenty-eight bacterial and Br transport experiments were performed in the field to determine the effects of physical and chemical heterogeneity of the aquifer sediment. The experiments were performed using groundwater from two field locations to examine the effects of groundwater chemistry on transport. Groundwater was extracted from multilevel samplers and pumped through 7-cm-long columns of intact sediment or repacked sieved and coated or uncoated sediment from the underlying aquifer. Two bacterial strains, Comamonas sp. DA001 and Paenibacillus polymyxa FER-02, were injected along with Br into the influent end of columns to examine the effect of cell morphology and cell surface properties on bacterial transport. The effects of column sediment grain size and mineral coatings coupled with groundwater geochemistry were also investigated. Significant irreversible attachment of DA001 was observed in the Fe oxyhydroxide-coated columns, but only in the suboxic groundwater where the concentrations of dissolved organic carbon (DOC) were ca. 1 ppm. In the oxic groundwater where DOC was ca. 8 ppm, little attachment of DA001 to the Fe oxyhydroxide-coated columns was observed. This indicates that DOC can significantly reduce bacterial attachment due electrostatic interactions. The larger and more negatively charged FER-02 displayed increasing attachment with decreasing grain size regardless of DOC concentration, and modeling of FER-02 attachment revealed that the presence of Fe and Al coatings on the sediment also promoted attachment. Finally, the presence of Al coatings and Al containing minerals appeared to significantly retard the Br tracer regardless of the concentration of DOC. These findings suggest that DOC in shallow oxic groundwater aquifers can significantly enhance the transport of bacteria by reducing attachment to Fe, Mn and Al oxyhydroxides. This effect appears to be profound for weakly and strongly charged hydrophilic bacteria and may contribute to differences in observations between laboratory experiments versus field-scale investigations particularly if the groundwater pH remains subneutral and Fe oxyhydroxide phases exist. These observation validate the novel approach taken in the experiments outlined here of performing laboratory-scale experiments on site to facilitate the use of fresh groundwater and thus be more representative of in situ groundwater conditions.     

Aspects of the biology of Derocheilocaris typica (Crustacea: Mystacocarida). II. Distribution

Mystacocarid crustaceans are characteristic members of the mesopsammon of many marine beaches. A detailed investigation of the distribution of Derocheilocaris typica Pennak and Zinn, 1943, shows that this species is most abundant on open ocean, high-energy beaches, with only scattered occurrence in estuarine beaches. A characteristic distribution pattern in beaches is shown, with maximum density occurring between mid and high-water levels. This pattern changes seasonally, with the position of maximum density moving deeper into the beach and more landward during winter months. Short-term changes in distribution also occur, resulting from tidal migrations and abnormal hydrodynamic patterns (i.e., storms). Longshore variability in abundance suggests the occurrence of clumps of crustaceans arranged much like beads on a string. Changes in abundance or segregation of sexes and stages seasonally are not found.     

Trill performance components vary with age, season, and motivation in the banded wren

Acoustic displays with difficult-to-execute sounds are often subject to strong sexual selection because performance levels are related to the sender’s condition or genetic quality. Performance may also vary with age, breeding stage, and motivation related to social context. We focused on within-male variation in four components of trill performance in banded wren (Thryophilus pleurostictus) songs: note consistency, frequency bandwidth, note rate, and vocal deviation. The latter is a composite measure reflecting deviation from the performance limit on simultaneously maximizing both frequency bandwidth and note rate. We compared the changes in these song parameters at three time scales: over the course of years, across the breeding season, and at different times of the day with contrasting agonistic contexts. Vocal deviation decreased and note consistency increased between years, suggesting that experience may improve individual proficiency at singing trills. Consistency also increased across the season, confirming that practice is important for this parameter. Although there was no significant seasonal change in vocal deviation, one of its components, note rate, increased during the season. Neither vocal deviation nor consistency varied with agonistic context. However, note rate increased during playback experiments simulating territorial intrusions compared to dawn chorus singing. The magnitude of a male’s increase in note rate was positively correlated with his aggressive behavior during the playback experiment. Thus consistency, bandwidth, and vocal deviation indicate age, whereas trill rate flexibly indicates the singer’s aggressive motivation. We also found evidence of a within-male trade-off between vocal deviation and consistency.     

Litter and dead wood dynamics in ponderosa pine forests along a 160-year chronosequence.

Disturbances such as fire play a key role in controlling ecosystem structure. In fire-prone forests, organic detritus comprises a large pool of carbon and can control the frequency and intensity of fire. The ponderosa pine forests of the Colorado Front Range, USA, where fire has been suppressed for a century, provide an ideal system for studying the long-term dynamics of detrital pools. Our objectives were (1) to quantify the long-term temporal dynamics of detrital pools; and (2) to determine to what extent present stand structure, topography, and soils constrain these dynamics. We collected data on downed dead wood, litter, duff (partially decomposed litter on the forest floor), stand structure, topographic position, and soils for 31 sites along a 160-year chronosequence. We developed a compartment model and parameterized it to describe the temporal trends in the detrital pools. We then developed four sets of statistical models, quantifying the hypothesized relationship between pool size and (1) stand structure, (2) topography, (3) soils variables, and (4) time since fire. We contrasted how much support each hypothesis had in the data using Akaike's Information Criterion (AIC). Time since fire explained 39-80% of the variability in dead wood of different size classes. Pool size increased to a peak as material killed by the fire fell, then decomposed rapidly to a minimum (61-85 years after fire for the different pools). It then increased, presumably as new detritus was produced by the regenerating stand. Litter was most strongly related to canopy cover (r2 = 77%), suggesting that litter fall, rather than decomposition, controls its dynamics. The temporal dynamics of duff were the hardest to predict. Detrital pool sizes were more strongly related to time since fire than to environmental variables. Woody debris peak-to-minimum time was 46-67 years, overlapping the range of historical fire return intervals (1 to > 100 years). Fires may therefore have burned under a wide range of fuel conditions, supporting the hypothesis that this region's fire regime was mixed severity.     

Duetting and mate-guarding in Australian magpie-larks (Grallina cyanoleuca)

A recently favored hypothesis is that duetting in birds has a mate-guarding function: a male responds vocally to his partner’s song, thereby forming a duet that repels males who are attracted to her song. Previous studies have not provided unambiguous tests of the mate-guarding hypothesis because: (1) the probability of a male answering his partner’s song has not been shown to increase specifically when the female is fertile, and (2) the probability of a male answering his partner’s song has not been assessed separately from simply a higher song initiation rate. We investigated extra-pair paternity, mate-guarding, and duetting in the socially monogamous Australian magpie-lark (Grallina cyanoleuca). DNA fingerprinting revealed that 3% of young were the result of extra-pair paternity, and we found that males guarded fertile females by staying close to them. However, males did not initiate songs at a higher rate when females were fertile and actually reduced their probability of replying to female song during this period. We conclude that although male magpie-larks did guard fertile females in an attempt to prevent extra-pair copulations, they did not use duetting for this purpose. Received: 10 May 1999 / Received in revised form: 27 September 1999 / Accepted: 2 October 1999