Biosensors for pathogen surveillance

Biologists, chemists, and physicists are collaborating to develop highly sensitive and specific biosensors for pathogen detection in the food, healthcare, and environmental sectors. Those novel biosensors allow quick detection and are thus expected to solve the issues of the emergence of highly virulent or antibiotic-resistant pathogens. This article reviews different types of biosensors used for pathogen detection, classified based on the type of transducer used. Optical biosensors integrate labeled means, e.g., fluorophores, quantum dots, and carbon dots to overcome photobleaching. Surface plasmon resonance is also used for enhanced sensitivity. Mechanical biosensors with piezoelectric crystals and cantilevers are adapted for the detection of food pathogens without sample preparation or labels. Conventional methods using electrodes for the measurement of electrochemical changes with differential pulse voltammetry or impedance spectroscopy are fast and highly sensitive. Immunosensors are developed for pathogen detection at trace levels using sample enrichment, signal amplification, and new visual detection techniques.     

Trace element retention and release on minerals and soil in a constructed wetland

Constructed wetlands are one method under investigation for the remediation of trace element-contaminated agricultural drainwater. A greater understanding of the retention of trace elements by the bulk soil and soil constituents is necessary for their safe and effective use. To determine the capacity of soil, calcite, and goethite-coated quartz sand for retention of As, Mo, and V under field conditions, an in situ method was used whereby permeable bags containing those minerals were placed near the sediment surface of a flow-through constructed wetland for 3 or 12 mo. Accumulations of As, Mo, and V occurred on goethite-coated sand. Concentrations of Mo on goethite-coated sand were much higher in samples from a wetland cell with a water depth of 15 cm (38.23 +/- 7.27 mg kg(-1)) compared with those from a cell with a water depth of 3 cm (8.30 +/- 1.45 mg kg(-1)). Calcite sorbed no As and low amounts of Mo and V, indicating that it is not an important sink for those elements under these conditions. In soil bags, total As and V concentrations showed little change over 12 mo. Molybdenum accumulated in the soil bags, resulting in total concentrations (12 mo) of 27.22 +/- 2.69 mg kg(-1) and 11.42 +/- 1.35 mg kg(-1) at water depths of 15 and 3 cm, respectively. Nearly half of the Mo accumulation on soil became water soluble after air-drying. This has important implications for systems that may undergo changes in redox status, possibly resulting in large fluxes of water-soluble Mo.     

STABLE HYDROGEN AND OXYGEN ISOTOPE COMPOSITION OF PRECIPITATION IN NORTHEASTERN COLORADO1

ABSTRACT: Where data are available, hydrologic studies may use precipitation's stable oxygen and hydrogen isotope composition to investigate streamflow, ground water/surface water interaction, and ground water recharge. Paleoclimate studies utilize the δ¹⁸O_{precipitation}‐T_air relationship, in conjunction with lake sediments, fossils, or old ground waters, for example, to estimate pale‐otemperatures. Ecological studies utilize precipitation and soil water isotope composition to track moisture uptake in plants, and to trace species migration patterns. Such studies require that the isotopic composition of precipitation be known. Oxygen‐18 (δ¹⁸O) and deuterium (δ²H) data for precipitation are lacking in the semi‐arid portion of the north‐central U.S. Great Plains, and thus there is a need to establish additional meteoric water lines as isotope input functions across the region, as well as to develop better understanding of the isotopic climate linkages that control oxygen and hydrogen isotope ratios in precipitation. This study determined the δ¹⁸O and δ²H composition of precipitation in the Pawnee Grasslands of northeastern Colorado from 1994 through 1998 using archived National Atmospheric Deposition Program samples. The resulting local water line follows the relationship δ²H = 7.86 δ¹⁸O‐7.66, and the data show a δ¹⁸O_weekly ‐ T_weekly relationship of δ¹⁸O = 0.560‐T (°C)‐18.8.     

Small mammal abundance and habitat relationships on deciduous forested sites with different susceptibility to gypsy moth defoliation

Small mammals are important predators of gypsy moths (Lymantria dispar L.), which are major defoliators of deciduous forests in the northeastern United States. Abundance and habitat relationships of small mammals were studied during summers 1984 and 1985 on forested sites at Moshannon and Rothrock state forests in two physiographic regions of Pennsylvania (Allegheny High Plateaus Province and Valley and Ridge Province, respectively) that varied in potential susceptibility to defoliation. The white-footed mouse (Peromyscus leucopus), which is a major vertebrate predator of gypsy moths, was the most common small mammal on all sites. Of the four common species, northern short-tailed shrews (Blarina brevicauda), southern red-backed voles (Clethrionomys gapperi), and white-footed mice were more abundant at Moshannon compared to Rothrock State Forest, but masked shrews (Sorex cinereus) were more abundant at Rothrock. Elevation was a major factor affecting abundance and distribution of small mammals. Because of the greater abundance of small mammals and more suitable physiographic features at Moshannon compared to Rothrock State Forest, small mammals may be more effective as predators on gypsy moths in the Allegheny High Plateaus than the Valley and Ridge Province of Pennsylvania.     

The International Environmental Expert Panel for Major Dam/Reservoir Projects: The Yellow River, China

The experience of the International Expert Panel on Environment for the Xiaolangdi dam/reservoir project in China (XEP) showed that the Panel meetings resulted in a marked improvement in project environmental performance by all parties participating in project implementation, with no parties exempt from the Panel's evaluations. Unfortunately the Loan Agreement authorizes use of the Panel only through the project stages of final design and construction, hence the 12th Panel meeting, which matched project construction completion, ended this series. The Panel has recommended that some meetings of the Panel be held during the project's operations stage in order to maintain continuing attention to environmental needs. The XEP experience showed that the XEP, in addition to monitoring project environmental performance, was very valuable for (i) training of Chinese staff involved in project implementation, and (ii) serving in the role as expert consultant for guiding the work of the Chinese participants. The XEP experience also showed the need for making a clear separation between evaluation, the overall resettlement program per se, and the environmental effects of the program. The Panel recommended that the Ministry of Water Resources and World Bank cooperate in sponsoring a book on The Environmental History of the Xiaolangdi Project, including both dam construction as well as resettlement problems, which would serve as a very valuable reference for guiding environmental planning for future dam projects in China (Ludwig, H.F. 1994–2000).     

Sediment delivery linkages in a chaparral watershed following a wildfire

The purpose of this research is to study the temporal and spatial sediment delivery to and within the stream network following a wildfire on a chaparral watershed in Arizona, USA. Methods include interpretation of channel processes (aggradation, degradation) from sequential aerial photographs, field measurements of sediment delivery, and overland flow from ten microwatersheds having different vegetation cover (no vegetation, chaparral cover, and bare with vegetation buffer strips). The response of the watershed to the fire was very complex. The fire reduced the chaparral cover to zero in most locations and severe erosion led to filling of the channels by sediment. With vegetation recovery, sediment delivery from the watershed practically ceased. Vegetation buffer strips were mainly responsible for arresting the sediment delivered from bare hillslopes. Relatively clear water, entering the channels, caused degradation in the tributaries that delivered the sediment into the main stream at El Oso Creek. Due to high water infiltration by immense volumes of sediment deposits in the middle reach, the sediment from the tributaries was deposited as in-channel fans. In contrast, the upper reach of El Oso Creek behaved similarly to the tributaries. It aggraded after the fire and was followed by degradation. The low reach of El Oso Creek is degrading because it is still adjusting base level to the incision of the master stream. Implications of this study are that land managers, concerned to avoid severe erosion and sedimentation following disturbance, should concentrate on the establishment and enhancement of vegetation buffer strips along channel banks.