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691.
Rowell FJ Sykes D Grieveson L Theaker B Sundar L Cumming RH 《Journal of environmental monitoring : JEM》2007,9(1):33-43
We describe the development and validation of a portable system comprising an air sampler coupled to an automated flow injection analysis device. The system is able to monitor airborne concentrations of subtilisin-type enzymes in the workplace atmosphere on a continuous basis. Sampling is in two stages: using a sampling head that is designed to mimic human respiration at approx. 1 m s(-1) at a sampling rate of 600 l min(-1). In the second stage, the captured particles are deposited by impaction from the air stream onto the inner surface of a cyclone that is continuously washed with a jet of buffer solution. Deposited particles are then washed into a reservoir from which samples are taken every 5-6 min and injected automatically into a continuous flow injection analysis system. Proteolytic enzyme in the sample passes through a bioreactor maintained at about 40 degrees C. This contains a cellulose solid phase matrix on which is covalently immobilised Texas Red-labelled gelatin as substrate. The passing enzyme partially digests the substrate releasing fluorophore that is detected down stream in a flow cell coupled to a fluorimeter. The system is calibrated using enzyme standards and the intensity of the resulting peaks from the ex-air samples is converted to airborne concentrations using a mathematical model programmed into a PC. The system has a limit of detection of 4.8 ng m(-3) and a dynamic range of 5-60 ng m(-3). The within assay precision (RSD) is 6.3-9.6% over this range. The within batch precision is 20.3% at 20 ng m(-3) and the corresponding between batch value is 19.5%. The system has been run for periods up to 8 h in the laboratory and for up to 4 h at a factory site and the values obtained compared with time-averaged values obtained from a conventional Galley sampler and in-house analysis when reasonable agreement of the results was observed. The stability of the system over 21 days of continuous use with standards injected periodically was studied. Linearity was observed for all the standard plots throughout. At the end of 21 days, after a total exposure equivalent to 2395 ng ml(-1) of Savinase, the signal due to the 5.0 ng ml(-1) standard was still easily detectable. 相似文献
692.
Characteristics that influence male reproductive success on a lek of Lethrinops c.f. parvidens (Teleostei: Cichlidae) 总被引:1,自引:1,他引:0
Sexual selection has long been proposed as a mechanism leading to the diverse cichlid (Teleostei: Cichlidae) fauna of Lake
Malawi, Africa. Many of the shallow-water, sand-dwelling, bower-building cichlid species are particularly well suited for
studies of sexual selection because they participate in leks. Since females in lekking systems appear to acquire only genetic
material from their mates, it has been suggested that leks are ideal systems to study female mate choice. The objectives of
the investigation were to examine Lethrinops c.f. parvidens male bower characteristics (i.e., bower size and location) as well as other male characteristics (i.e., length, gular color,
and duration on the lek) for their influence on male mating success as measured by the number of visits, circles, and eggs
laid by females. These measures are nested in that a visit by a female may or may not lead to circling, and circling by a
female may or may not lead to egg-laying. We found increased bower height and higher numbers of conspecific neighbors (analogous
to shallow-water, near-shore bower positions) to be positively, significantly associated with the number of visits by females.
The only significant correlate with the number of circles was visits, and similarly circles was the only significant correlate
with the number of eggs laid. The R
2 value for the egg-laying regression was quite low (19.8%) compared with visits (54.3%) and circling (78.9%), suggesting that
females may be using additional cues, that we failed to measure, when in close proximity to males or simply that a small proportion
of the females were ready to spawn. Both indirect selection and direct selection pressure due to egg predation may have influenced
female choice on the lek.
Received: 10 April 1999 / Received in revised form: 26 July 1999 / Accepted: 18 September 1999 相似文献
693.
Male willow warblers have song repertoires which vary in complexity along several dimensions. We examined whether female choice,
as measured by date of pairing, was based on these song characteristics in 4 different years. Pairing date was negatively
correlated with song repertoire size in 1 year, and with song versatility in another year, but there was no consistent effect
of any song characteristic on pairing throughout the years or in the pooled sample. The variable that best explained how soon
a male pairs is male arrival date (only males that had settled territories before the first female arrived were considered
in the analysis). This correlation is consistently significant in all years. This is most parsimoniously interpreted as females
choosing some habitat characteristic in the same way that males do. A small percentage of males (8.3%) attracted and paired
with a second female. The likelihood of becoming polygynous was not explained by any measured song characteristic, but it
was related to arrival date: early males were more likely to pair with two females. Males with large repertoires fledged more
young in their primary nests, and there was a trend for the offspring of these males to have a greater probability of being
recruited into the population. In conclusion, the results show that in most years there is no sexual selection by female preference
on song characteristics, although the data on reproductive success is consistent with the idea of repertoire size being an
indicator of male quality.
Received: 4 June 1999 / Received in revised form: 1 December 1999 / Accepted: 31 December 1999 相似文献
694.
T. Lubjuhn Wolfgang Winkel Jörg Thomas Epplen Jörg Brün 《Behavioral ecology and sociobiology》2000,48(1):12-17
One of the most interesting aspects of the mating system of pied flycatchers is the regular occurrence of polygyny. Here we
present data on the reproductive success of polygynously paired pied flycatcher males compared to monogamous males based on
paternity analyses through DNA fingerprinting. Males paired with two females suffered a higher loss in reproductive output
per female compared to monogamous males due to (1) a greater proportion of unhatched eggs in their broods, (2) greater nestling
mortality and (3) a greater probability of being cuckolded. Nevertheless, the number of fledglings was significantly greater
for polygynous males. Based on the number of nestlings that returned for subsequent breeding seasons, however, the reproductive
success of monogamous and polygynous males did not differ significantly. These data raise the question as to why males attempt
polygyny.
Received: 16 August 1999 / Received in revised form: 15 March 2000 / Accepted: 18 March 2000 相似文献
695.
Life history costs of olfactory status signalling in mice 总被引:4,自引:0,他引:4
L. M. Gosling S. C. Roberts E. A. Thornton M. J. Andrew 《Behavioral ecology and sociobiology》2000,48(4):328-332
Large body size confers a competitive advantage in animal contests but does not always determine the outcome. Here we explore
the trade-off between short-term achievement of high social status and longer-term life history costs in animals which vary
in competitive ability. Using laboratory mice, Mus musculus, as a model system, we show that small competitors can initially maintain dominance over larger males by increasing investment
in olfactory status signalling (scent-marking), but only at the cost of reduced growth rate and body size. As a result they
become more vulnerable to dominance reversals later in life. Our results also provide the first empirical information about
life history costs of olfactory status signals.
Received: 15 December 1999 / Revised: 6 June 2000 / Accepted: 24 June 2000 相似文献
696.
Utility of North Atlantic Right Whale Museum Specimens for Assessing Changes in Genetic Diversity 总被引:2,自引:0,他引:2
Howard C. Rosenbaum †§§ Mary G. Egan Phillip J. Clapham ‡ Robert L. Brownell JR. § Sobia Malik Moira W. Brown ††‡‡ Bradley N. White Peter Walsh † and Rob Desalle 《Conservation biology》2000,14(6):1837-1842
Abstract: We examined six historical specimens of the endangered North Atlantic right whale ( Eubalaena glacialis ) using DNA isolated from documented baleen plates from the late nineteenth and early twentieth centuries. Sequences from the mitochondrial DNA (mtDNA) control region from these samples were compared with those from a near-exhaustive survey (269 of approximately 320 individuals) of the remaining right whales in the western North Atlantic Ocean. Our results suggest that there has been only relatively modest change in maternal lineage diversity over the past century in the North Atlantic right whale population. Any significant reduction in genetic variation in the species most likely occurred prior to the late nineteenth century. One historical specimen was from the last documented female capable of propagating one of the maternal lineages in the population today; no females in the existing population have been found to carry this mtDNA haplotype. Analysis of the only specimens from the eastern North Atlantic right whale population ever to be examined revealed that eastern and western North Atlantic right whales may not have been genetically differentiated populations. Loss of gene diversity experienced by North Atlantic right whales over the last century has been modest, and the six decades of protection have been successful in maintaining much of the maternal lineage diversity that was present in the late nineteenth century. 相似文献
697.
Assessing the Geographic Representativeness of Genebank Collections: the Case of Bolivian Wild Potatoes 总被引:5,自引:0,他引:5
R. J. Hijmans K. A. Garrett Z. Huamán D. P. Zhang M. Schreuder M. Bonierbale 《Conservation biology》2000,14(6):1755-1765
Abstract: Genebank collection databases can be used for ecogeographical studies under the assumption that the accessions are a geographically unbiased sample. We evaluated the representativeness of a collection of wild potatoes from Bolivia and defined and assessed four types of bias: species, species-area, hotspot, and infrastructure. Species bias is the sampling of some species more often than others. Species-area bias is a sampling that is disproportionate to the total area in which a species is found. Hotspot bias is the disproportionate sampling of areas with high levels of diversity. Infrastructure bias is the disproportionate sampling of areas near roads and towns. Each of these biases is present in the Bolivian wild potato collection. The infrastructure bias was strong: 60% of all wild potato accessions were collected within 2 km of a road, as opposed to 22%, if collections had been made randomly. This analysis can serve as a guide for future collecting trips. It can also provide baseline information for the application of genebank data in studies based on geographic information systems. 相似文献
698.
Abstract: Detrimental effects of introduced fishes on native amphibian populations have prompted removal of introduced cutthroat ( Oncorhynchus clarki ), rainbow ( Oncorhynchus mykiss ), and brook trout ( Salvelinus fontinalis ) from naturally fishless lakes at Mt. Rainier National Park, Washington ( U.S.A.). Using paleolimnological indicators (diatoms, invertebrates, and sediment characteristics) in eight 480-year-old sediment cores from eight lakes, we (1) derived estimates of baseline environmental conditions and natural variation, (2) assessed the effects of stocking naturally fishless lakes, and (3) determined whether lakes returned to predisturbance conditions after fish removal (restoration). Diatom floras were relatively stable between 315 and 90 years before present in all lakes; we used this time period to define lake-specific "baseline" conditions. Dissimilarity analyses of diatoms revealed sustained, dramatic changes in diatom floras that occurred approximately 80 years ago (when fish were introduced) in four of five stocked lakes, whereas the diatom floras in two unstocked lakes had not changed significantly in the last 315 years. Diatoms were not preserved in an eighth lake. State changes also occurred in two lakes over 200 years before European settlement of the Pacific Northwest. Preserved invertebrate densities fluctuated dramatically over time in all cores, providing a poor reference for assessing the effects of fishes. Nevertheless, fish-invertebrate interactions have been demonstrated in other paleolimnological studies and may be useful for lower-elevation or more productive lakes. Because diatom communities have not returned to predisturbance assemblages in restored lakes, even 20–30 years after fish removal, we conclude that Mt. Rainier lakes were not successfully restored by the removal of fishes. 相似文献
699.
Abstract: Understanding how the fauna of logged tropical rainforests responds to fragmentation and the creation of edges is vital to ensure conservation of biodiversity. We studied the composition of the understory bird community from the edge of a 15-ha clearing toward the interior of the forest in a part of Budongo Forest Reserve, Uganda, that was selectively logged about 45 years ago. Mist netting was conducted along five transects from the edge and 500 m into the interior. The total number of individuals captured did not change with distance from the edge, but there was a significant increase in the number of species. We sampled fewer, but more common species near the edge, whereas the interior of the forest had more, and less common species. Guild composition also changed with distance from the edge. Frugivore-insectivores and nectarivores were most common close to the edge. Among insectivores, ground foragers, bark-gleaners, and leaf-gleaners were most common in the interior of the forest, whereas sallying insectivores favored the edge. Graminivores were unaffected by the edge. Analysis of common species showed that Ispidina picta , Andropadus curvirostris , A. latirostris , Camaroptera brachyura , Terpsiphone rufiventer , and Nectarinia olivacea were associated with the edge, but no species showed significant avoidance of the edge. This finding may be explained by the generally low sample sizes of interior species. Our results show that even bird communities in logged forests respond to edges. Estimates of edge effects suggested that changes in bird densities may have occurred several hundred meters from the edge. In conclusion, logged forests provide habitat for bird species avoiding forest edges, and this should be considered in the management of such forests for conservation. 相似文献
700.