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21.
Laura Leite Florence Jude-Lemeilleur Natalie Raymond Isabel Henriques Frédéric Garabetian Artur Alves 《Environmental science and pollution research international》2017,24(27):21721-21732
According to the hologenome theory, the microbiota contributes to the fitness of the holobiont having an important role in its adaptation, survival, development, health, and evolution. Environmental stress also affects the microbiota and its capability to assist the holobiont in coping with stress factors. Here, we analyzed the diversity of cultivable bacteria associated with Manila clam tissues (mantle, gills, hemolymph) in two non-contaminated sites (Portugal and France) and one metal-contaminated site (Portugal). A total of 240 isolates were obtained. Representative isolates (n = 198) of the overall diversity were identified by 16S rDNA sequencing and subjected to functional characterization. Isolates affiliated with Proteobacteria, Actinobacteria, Firmicutes, and Bacteroidetes. Proteobacteria (mostly Pseudoalteromonadaceae and Vibrionaceae) were dominant in non-contaminated sites while Actinobacteria (mostly Microbacteriaceae) dominated in the metal-contaminated site. The main factor affecting the microbiota composition was contamination. No significant differences were observed between clam tissues and geographic regions. Several isolates tested positive for antibacterial activity, biofilm formation, protease, and siderophore production. The results show that the Manila clam harbors a diverse microbiota that may contribute to clam protection and overall fitness, as well as to its adaptation to stressful environments. In addition, the Manila clam microbiota is revealed as a promising source of novel probiotics with potential application in aquaculture. 相似文献
22.
Inês Ribeiro Jörg Kaufmann Uwe Götze Paulo Peças Elsa Henriques 《International Journal of Sustainable Engineering》2019,12(3):201-211
Technological development has had huge impacts in sports performance throughout the years, fostering the development of specific materials and manufacturing processes for sports-related products. Within this context, a new technology for snowboards was developed taking advantage of the internal coupling effects of Fibre Reinforced Plastics (FRP) using an anisotropic layer design. This work deals with the technical, economic and environmental evaluation of a snowboard made of three alternative materials, namely carbon, glass and flax fibre reinforced plastics. It shows how a life cycle analysis can support the design and development of products by applying a Life Cycle Engineering methodology to the design process of a snowboard using an innovative technology. In this case, both the material choice and the fiber placement angles have a significant impact on the stiffness of the resulting FRP and thus in the three dimensions of analysis - cost, environmental and technical. The natural fiber is the most sustainable option environmentally, the glass fiber is the best one economically and the carbon fibre is the best in terms of technical performance. Therefore, the importance attributed to each dimension of analysis is pondered and traded-off to allow an aggregated analysis of the alternatives and a well-informed decision-making. 相似文献
23.
Capacity Factor Analysis is a decision support system for selection of appropriate technologies for municipal sanitation services in developing communities. Developing communities are those that lack the capability to provide adequate access to one or more essential services, such as water and sanitation, to their residents. This research developed two elements of Capacity Factor Analysis: a capacity factor based classification for technologies using requirements analysis, and a matching policy for choosing technology options. First, requirements analysis is used to develop a ranking for drinking water supply and greywater reuse technologies. Second, using the Capacity Factor Analysis approach, a matching policy is developed to guide decision makers in selecting the appropriate drinking water supply or greywater reuse technology option for their community. Finally, a scenario-based informal hypothesis test is developed to assist in qualitative model validation through case study. Capacity Factor Analysis is then applied in Cimahi Indonesia as a form of validation. The completed Capacity Factor Analysis model will allow developing communities to select drinking water supply and greywater reuse systems that are safe, affordable, able to be built and managed by the community using local resources, and are amenable to expansion as the community's management capacity increases. 相似文献