Brominated flame retardants and other organochlorine pollutants in human adipose tissue samples from the Czech Republic

Brominated flame retardants (BFRs) represented by polybrominated diphenyl ethers (PBDEs) and hexabromocyclododecane (HBCD) together with major persistent organochlorine pollutants, polychlorinated biphenyls (PCBs) and selected organochlorine pesticides (OCPs), were determined in adipose tissue samples (n=98) obtained by liposuction of Czech subjects. Compared to other organochlorine pollutants (mostly PCBs and DDTs), levels of PBDE were lower by 2 orders of magnitude ranging from 0.2 to 54.3 ng/g lipid weight. PBDE congeners No. 47, 99, 153 and 183 were the most abundant constituting up to 90% of these pollutants in adipose tissue. The PBDEs content measured in this study was comparable with data reported in similar samples collected in Spain, Sweden, Belgium and Japan, whilst slightly lower than in the United States. Regarding PCBs, the dominating congeners were No. 138, 153 and 180 representing up to 90% of indicator congeners. The levels of PCBs were similar to those found in other European countries. While no age dependency was found for PBDEs, an increase of PCB and OCP levels with age was observed. Different exposure routes of donors were documented by the absence of the relationship between PCBs and OCPs.     

Global temperature change from the transport sectors: Historical development and future scenarios

Transport affects climate directly and indirectly through mechanisms that operate on very different timescales and cause both warming and cooling. We calculate contributions to the historical development in global mean temperature for the main transport sectors (road transport, aviation, shipping and rail) based on estimates of historical emissions and by applying knowledge about the various forcing mechanisms from detailed studies. We also calculate the development in future global mean temperature for four transport scenarios consistent with the IPCC SRES scenarios, one mitigation scenario and one sensitivity test scenario. There are large differences between the transport sectors in terms of sign and magnitude of temperature effects and with respect to the contributions from the long- and short-lived components. Since pre-industrial times, we calculate that transport in total has contributed 9% of total net man-made warming in the year 2000. The dominating contributor to warming is CO₂, followed by tropospheric O₃. By sector, road transport is the largest contributor; 11% of the warming in 2000 is due to this sector. Likewise, aviation has contributed 4% and rail ～1%. Shipping, on the other hand, has caused a net cooling up to year 2000, with a contribution of ?7%, due to the effects of SO₂ and NOx emissions. The total net contribution from the transport sectors to total man-made warming is ～15% in 2050, and reaches 20% in 2100 in the A1 and B1 scenarios. For all scenarios and throughout the century, road transport is the dominating contributor to warming. Due to the anticipated reduction in sulphur content of fuels, the net effect of shipping changes from cooling to warming by the end of the century. Significant uncertainties are related to the estimates of historical and future net warming mainly due to cirrus, contrails and aerosol effects, as well as uncertainty in climate sensitivity.     

The diagnostic performance of cytogenetic investigation in amniotic fluid cells and chorionic villi

First-trimester chorionic villus sampling has not reached the popularity of second-trimester amniocentesis in prenatal cytogenetic diagnosis, in contrast to initial expectations. We investigated whether a difference inthe diagnostic performances of cytogenetic investigation in amniotic fluid (AF) cells and chorionic villi in favour of AF-cells might justify this. Diagnostic performance was measured as laboratory failure rate, karyotype quality (G-band score, rate of follow-up samples, rate of wrong diagnoses), and karyotype representativity (rate of follow-up samples, rate of wrong diagnoses). From 1993–1999, 11 883 AF-samples were investigated (AF-cells). In chorionic villi, short term culture preparations solely were karyotyped from 1993–1996 (n=3499) (STC-villi), short and long-term culture preparations simultaneously provided a sufficient amount of tissue being available from 1997 onwards (n=1829) ((STC+LTC)-villi). Laboratory failure rates were the same after amniocentesis (0.40%) and chorionic villus sampling (0.50%). G-band scores (mean±SD) were equal in AF-cells (373±38.1) and LTC-villi (364±32.6) but significantly lower in STC-villi (311±34.6) (p=0.001). Follow-up sampling rates because of quality reasons were the same in AF-cells (0.14%), STC- villi (0.13%) and (STC+LTC)-villi (0.11%). Two wrong diagnoses turned up among AF-cells. Follow-up sampling rates because of representativity reasons differed significantly between AF-cells (0.10%), (STC+LTC)-villi (1.31%), and STC-villi (1.99%) (p<0.001). However, the ratios of the total numbers of follow-up samples and uncertain or abnormal cytogenetic results in STC, and (STC+LTC)-villi at cytogenetic risks ⩾3% (0.132 and 0.160, respectively) were equal to that in AF-cells at risks <3% (0.155). Two wrong diagnoses were made in STC-villi. Diagnostic performance improved in the rank order of STC-villi, (STC+LTC)-villi and AF-cells. At cytogenetic risks ⩾3%, (STC+LTC)-villi showed a diagnostic performance equal to that in AF-cells. This might justify a selective use of chorionic villus sampling. Copyright © 2001 John Wiley & Sons, Ltd.     

Current controversies in prenatal diagnosis 1: NIPT for chromosome abnormalities should be offered to women with low a priori risk

In its successful annual cycle of controversies and debates, the International Society of Prenatal Diagnosis and Therapy once again addressed non-invasive prenatal testing (NIPT) by following up on the 2013 controversy, ‘Should non-invasive DNA testing be the standard screening test for Down syndrome in all pregnant women’? with the proposition, ‘NIPT for chromosomel abnormalities should be offered to women with low a priori risk’. © 2014 John Wiley & Sons, Ltd.     

Occupational stress in (inter)action: the interplay between job demands and job resources

The present study addresses theoretical issues involving different interaction effects between job demands and job resources, accompanied by a thorough empirical test of interaction terms in the demand–control (DC) model and the effort–reward imbalance (ERI) model in relation to employee health and well‐being (i.e., exhaustion, psychosomatic health complaints, company‐registered sickness absence). Neither the DC model nor the ERI model gives a clear theoretical rationale or preference for a particular interaction term. Hierarchical regression analyses were conducted among 405 nursing home employees and cross‐validated in a comparable sample (N = 471). Results including cross‐validation showed that only a multiplicative interaction term yielded consistent results for both the DC model and the ERI model. Theoretical as well as empirical results argue for a multiplicative interaction term to test the DC model and the ERI model. Future job stress research may benefit from the idea that there should be a theoretical preference for any interaction form, either in the DC model or in the ERI model. However, more research on interactions is needed to address this topic adequately. Copyright © 2005 John Wiley & Sons, Ltd.