A temperature-profile method for estimating flow in geologic heat pipes

Above-boiling temperature conditions, as encountered, for example, in geothermal reservoirs and in geologic repositories for the storage of heat-producing radioactive wastes, may induce strong liquid and gas flow processes in porous subsurface environments. The magnitude of these flow processes is extremely hard to measure in the field. We therefore propose a simple temperature-profile method that uses high-resolution temperature data for deriving such information. The energy that is transmitted with the vapor and water flow creates a nearly isothermal zone maintained at about the boiling temperature, referred to as a heat pipe. Characteristic features of measured temperature profiles, such as the differences in the gradients inside and outside of the heat-pipe regions, are used to derive the approximate magnitude of the liquid and gas fluxes in the subsurface.     

Mineralisation of 2,6-dichlorobenzamide (BAM) in dichlobenil-exposed soils and isolation of a BAM-mineralising Aminobacter sp

Mineralisation of the groundwater contaminant 2,6-dichlorobenzamide (BAM), a metabolite from the herbicide 2,6-dichlorobenzonitrile (dichlobenil), was studied in soil samples obtained from 39 locations previously exposed to dichlobenil. Rapid BAM mineralisation was detected in samples from six locations with 5.2-64.6% of the added BAM mineralised within 48-50 days. From one location rapid BAM mineralisation was observed in soil samples down to a depth of 2 m below the surface. One location with fast BAM mineralisation showed significant dichlobenil degradation activity with 25.5% of the added dichlobenil being mineralised within 50 days. By inoculating soil showing the fastest mineralisation of BAM into a mineral medium with BAM as the only carbon and nitrogen source an enrichment culture was established. Community analysis based on extracted DNA revealed a change of the bacterial community but without any clear indication of key members within the BAM-mineralising culture. Parallel cultivation resulted for the first time in the isolation of a BAM-mineralising bacterium, identified as an Aminobacter sp.     

Nitrogen and phosphorus budgets in experimental grasslands of variable diversity

Previous research has shown that plant diversity influences N and P cycles. However, the effect of plant diversity on complete ecosystem N and P budgets has not yet been assessed. For 20 plots of artificially established grassland mixtures differing in plant diversity, we determined N and P inputs by bulk and dry deposition and N and P losses by mowing (and subsequent removal of the biomass) and leaching from April 2003 to March 2004. Total deposition of N and P was 2.3 +/- 0.1 and 0.2 +/- 0.01 g m(-2) yr(-1), respectively. Mowing was the main N and P loss. The net N and P budgets were negative (-6.3 +/- 1.1 g N and -1.9 +/- 0.2 g P m(-2) yr(-1)). For N, this included a conservative estimate of atmospheric N(2) fixation. Nitrogen losses as N(2)O were expected to be small at our study site (<0.05 g m(-2) yr(-1)). Legumes increased the removal of N with the harvest and decreased leaching of NH(4)-N and dissolved organic nitrogen (DON) from the canopy. Reduced roughness of grass-containing mixtures decreased dry deposition of N and P. Total dissolved P and NO(3)-N leaching from the canopy increased in the presence of grasses attributable to the decreased N and P demand of grass-containing mixtures. Species richness did not have an effect on any of the studied fluxes. Our results demonstrate that the N and P fluxes in managed grassland are modified by the presence or absence of particular functional plant groups and are mainly driven by the management.     

Social recognition in sticklebacks: the role of direct experience and habitat cues

The social organisation of animals relies on recognition. However, there are many means by which animals may recognise one another and a variety of cues are available to any individual at any one time. We tested the effects of cues based on direct experience and of cues based on habitat and diet on association decisions in the three-spined stickleback (Gasterosteus aculeatus). Our results show that sticklebacks rapidly acquire association preferences for novel con- and heterospecifics (nine-spined sticklebacks, Pungitius pungitius) which had experienced the same habitat and diet conditioning as themselves over novel con- and heterospecifics which had experienced a different habitat and diet conditioning, a preference which may be based on self-referent matching. Association preferences were observed after only 24 h and were independent of the number of tank mates (treatments with 20 and 100 fish). Evidence for the influence of direct social experience on association preferences was weak and may be confounded by other factors.Communicated by K. Lindström     

Extensive cross-disciplinary analysis of biological and chemical control of Calanus finmarchicus reproduction during an aldehyde forming diatom bloom in mesocosms

Egg and faecal pellet production and egg hatching success of the calanoid copepod Calanus finmarchicus were monitored over a period of 14?days (14?C28 April, 2008) while fed water from 4 differently treated mesocosms and ambient water. Two of the mesocosms used were inoculated with the polyunsaturated aldehyde (PUA)-producing diatom Skeletonema marinoi, while 2 received only nutrient additions with or without silica. The mesocosms developed blooms of S. marinoi, mixed diatoms or the haptophyte Phaeocystis pouchetii, respectively. Faecal pellet production of C. finmarchicus increased with increasing food availability. Egg production increased with time in all mesocosms to a maximum single female production of 232 eggs female^?1?day^?1 (average of 90 eggs female^?1?day^?1) and followed the development of ciliates and P. pouchetii, but was not affected by the observed high (up to 15?nmol?L^?1) PUA production potential of the phytoplankton. The hatching success of the eggs produced on the mesocosm diets was high (78?C96%) and was not affected by either aldehydes in the maternal diet or exposure to the dissolved aldehydes in the water.     

Humans use social information to adjust their quorum thresholds adaptively in a simulated predator detection experiment

Quorum sensing is used in many biological systems to increase decision accuracy. In quorum sensing, the probability that an individual adopts a behavior is a nonlinear function of the number of other individuals adopting this behavior. From an optimal decision-making perspective, individuals should adjust their quorum threshold to the particulars of the decision problem. Recent work predicts that a key factor here is the quality of social information. In particular, it is predicted that individuals should adjust their quorum thresholds such that it lies in between the average true and false positive rate of the other group members. We here test this prediction with a predator detection experiment. First, human groups observed a group of animals (projected on a white screen) in which a predator was present or absent, and each individual made an independent decision to escape or not. Second, individuals received social information on the decisions of their group members, after which individuals decided again. This social information, however, did not represent their own decisions but consisted of responses that either came from a high-performing group (i.e., many individuals detecting the predator) or from a low-performing group (i.e., few individuals detecting the predator). We found that individuals adaptively adjust their quorum threshold to the quality of the social information: when receiving social information from high-performing groups, individuals employed higher quorum thresholds than when receiving information from low-performing groups. Our study demonstrates that humans can quickly evaluate the quality of publicly available information and adaptively adjust their decision rules.     

Effects of ocean acidification, temperature and nutrient regimes on the appendicularian Oikopleura dioica: a mesocosm study

Increasing pCO₂ is hypothesized to induce shifts in plankton communities toward smaller cells, reduced carbon export rates and increased roles of gelatinous zooplankton. Appendicularians, among the most numerous pan-global “gelatinous” zooplankton, continuously produce filter-feeding houses, shortcutting marine food webs by ingesting submicron particles, and their discarded houses contribute significantly to carbon fluxes. We present a first mesocosm-scale study on the effects of temperature, pCO₂ and bloom structures on the appendicularian, Oikopleura dioica. There were effects of temperature and nutrients on phytoplankton communities. No shifts in functional phytoplankton groups, nor changes in particle sizes/morphotypes, known to impact appendicularian feeding, were observed under manipulated pCO₂ conditions. However, appendicularian abundance was positively correlated with increased pCO₂, temperature and nutrient levels, consistent with hypotheses concerning gelatinous zooplankton in future oceans. This suggests appendicularians will play more important roles in marine pelagic communities and vertical carbon transport under projected ocean acidification and elevated temperature scenarios.     

Histology of Sculpin spp. in East Greenland. II. Histopathology and trace element concentrations

Abstract

For many years, the sculpin has been utilized as a sentinel monitoring species for anthropogenic impacts on the marine environment. To further develop its potential as a screening model body burden of several trace elements, including cadmium (Cd), copper (Cu), mercury (Hg), lead (Pb) and selenium (Se), were investigated. In addition, histopathology in shorthorn sculpins (Myoxocephalus scorpius; n?=?20) and fourhorn sculpins (Myoxocephalus quadricornis; n?=?10) were examined at three sites around the Scoresby Sound settlement in Central East Greenland. Fourhorn sculpins caught at the most distant site from the settlement, contained significantly higher hepatic Cu and Cd concentrations as well as significantly elevated gill Pb levels compared to the shorthorn sculpins collected at two other sites closer to the settlement. Histological examinations showed that fish with significantly higher concentrations of hepatic Cd and Se exhibited greater nuclear alterations, interstitial hyperplasia/hypertrophy, interstitial mononuclear infiltrations and granulomas. Further, fish with higher Cd and Cu gill tissue levels displayed a significantly higher number of cytoplasmic alterations and lamellar epithelium lifting, hypertrophic and hyperplastic epithelium along with mucus cell hyperplasia. While the presence of liver lesions were not species or sex-specific, the presence of gill lesions decreased in the order female fourhorn sculpins?>?female shorthorn sculpins?>?male fourhorn sculpins?>?male shorthorn sculpins. Hepatic Hg concentrations exceeded known lowest observable effect doses (LOED) for fish (0.1–0.5?μg g^?1 ww) in 27% of fish, while liver Cd residues in 80% exceeded LOED (0.42–1.8?μg g^?1 ww). Based upon these results, data suggest that using the sculpin as a valuable sentinel fish species histopathology may serve as a reliable tool for assessing marine ecosystem exposure to trace metals. However, confounding physiological and ecological factors also need to be considered.