Determination of hypoxia and dietary copper mediated sub-lethal toxicity in carp, Cyprinus carpio, at different levels of biological organisation

Hypoxic events frequently occur in the aquatic environment in association with micro pollutants, including heavy metals. Only a few studies are however available on the uptake and biological responses of heavy metals under hypoxic conditions. To elucidate the phenomenon, mirror carp Cyprinus carpio L. (16.13-16.22 g) were exposed chronically to dietary copper (Cu; 250 and 500 mg kg dry wt.⁻¹) for 30 d under normoxic (8.25 mg O₂ L⁻¹) and hypoxic (∼3 mg O₂ L⁻¹) conditions and adopting an integrated approach, sub-lethal biomarker responses were determined at different levels of biological organisation. Level of oxidative DNA damage (as determined by modified Comet assay) showed strong significant difference following exposure to dietary Cu level under normoxic (1.6-fold) as well as under hypoxic condition at both Cu levels (2.1 and 2.5-folds respectively). Significant difference was also observed for haematological parameters (i.e. increased red and white blood cells, haematocrit value and haemoglobin concentration). Quantitative histology revealed alterations in tissues (i.e. liver and gills) for hypoxic and all dietary Cu treatment groups under both normoxic and hypoxic conditions suggesting a compensatory response to these organs (p < 0.05). The order of Cu accumulation in tissues (as determined by ICP-OES) was liver > intestine > kidney > gill. Interestingly, SGR under both normoxic and hypoxic conditions reduced with elevating Cu levels (p = 0.019). Overall, the results provide evidence for enhanced toxicological responses in fish following exposure to Cu either alone or in combination with hypoxic condition and lends support to the evolving viewpoint that many water quality guidelines should be revisited in terms of new ecotoxicological criteria.     

Intake of ²³⁸U and ²³²Th through the consumption of foodstuffs by tribal populations practicing slash and burn agriculture in an extremely high rainfall area

The concentration of naturally occurring radionuclides ²³²Th, ²³⁸U was determined using Instrumental Neutron Activation Analysis (INAA) in different food groups namely cereals, vegetables, leafy vegetables, roots and tubers cultivated and consumed by tribal population residing around the proposed uranium mine. The study area is a part of rural area K. P. Mawthabah (Domiasiat) in the west Khasi Hills District of Meghalaya, India located in the tropical region of high rainfall that remains steeped in tribal tradition without much outside influence. Agriculture by Jhum (slash and burn) cultivation and animal husbandry are the main occupation of the tribal populations. A total of 89 samples from locally grown food products were analyzed. The concentration of ²³⁸U and ²³²Th in the soil of the study area was found to vary 1.6-15.5 and 2.0-5.0 times respectively to the average mean value observed in India. The estimated daily dietary intake of ²³⁸U and ²³²Th were 2.0 μg d⁻¹ (25 mBq d⁻¹) and 3.4 μg d⁻¹ (14 mBq d⁻¹) is comparable with reported range 0.5-5.0 μg d⁻¹ and 0.15-3.5 μg d⁻¹ respectively for the Asian population.     

Solvent tolerant marine bacterium Bacillus aquimaris secreting organic solvent stable alkaline cellulase

The organic solvent tolerant bacteria with their physiological abilities to decontaminate the organic pollutants have potentials to secrete extracellular enzymes of commercial importance. Of the 19 marine bacterial isolates examined for their solvent tolerance at 10 vol.% concentration, one had the significant tolerance and showed a relative growth yield of 86% for acetone, 71% for methanol, 52% for benzene, 35% for heptane, 24% for toluene and 19% for ethylacetate. The phylogenetic analysis of this strain using 16S rDNA sequence revealed 99% homology with Bacillus aquimaris. The cellulase enzyme secreted by this strain under normal conditions showed an optimum activity at pH 11 and 45 °C. The enzyme did show functional stability even at higher pH (12) and temperature (75 °C) with residual activity of 85% and 95% respectively. The enzyme activity in the presence of different additives were in the following order: Co⁺² > Fe⁺² > NaOCl₂ > CuSO₄ > KCl > NaCl. The enzyme stability in the presence of solvents at 20 vol.% concentration was highest in benzene with 122% followed by methanol (85%), acetone (75%), toluene (73%) and heptane (42%). The pre-incubation of enzyme in ionic liquids such as 1-ethyl-3-methylimidazolium methanesulfonate and 1-ethyl-3-methylimidazolium bromide increased its activity to 150% and 155% respectively. The change in fatty acid profile with different solvents further elucidated the physiological adaptations of the strain to tolerate such extreme conditions.     

The Economic and Environmental Outcomes of Microfinance Projects: An Indian Case Study

Microfinance projects now seek to achieve improvement in environmental quality in addition to poverty alleviation. Achievement of these goals may depend upon the economic and environmental impact of microfinance businesses. This study is a quantitative analysis of these outcomes for Ecodevelopment, an Indian microfinance project, which aims to prevent forest degradation by poverty alleviation. The study divides businesses into perishable goods, non-perishable goods, simple skills, and special skills and measures their economic outcome by two indicators, repayment percentage and continuity of businesses. The study examines the effect of loan amount on the economic outcome of businesses. To quantify the environmental outcome, it analyzes the use of firewood or fodder and release of pollutants by businesses. The results show that loan amount does not affect the economic outcome, special skills have best performance, and skills cause less pressure on forest resources. These results suggest the need for development of knowledge-intensive skills, involvement of institutions in the operations of business, and inclusive policies for protected area management.     

Modeling the energy content of combustible ship-scrapping waste at Alang-Sosiya, India, using multiple regression analysis

Alang-Sosiya is the largest ship-scrapping yard in the world, established in 1982. Every year an average of 171 ships having a mean weight of 2.10 x 10(6)(+/-7.82 x 10(5)) of light dead weight tonnage (LDT) being scrapped. Apart from scrapped metals, this yard generates a massive amount of combustible solid waste in the form of waste wood, plastic, insulation material, paper, glass wool, thermocol pieces (polyurethane foam material), sponge, oiled rope, cotton waste, rubber, etc. In this study multiple regression analysis was used to develop predictive models for energy content of combustible ship-scrapping solid wastes. The scope of work comprised qualitative and quantitative estimation of solid waste samples and performing a sequential selection procedure for isolating variables. Three regression models were developed to correlate the energy content (net calorific values (LHV)) with variables derived from material composition, proximate and ultimate analyses. The performance of these models for this particular waste complies well with the equations developed by other researchers (Dulong, Steuer, Scheurer-Kestner and Bento's) for estimating energy content of municipal solid waste.     

A study of the technologically modified sources of 222Rn and its environmental impact in an Indian U mineralised belt

These investigations present qualitative and quantitative assessments of the 222Rn contributing potential of the technologically modified sources, e.g. mine exhausts and tailings piles, arising due to the mining and processing of uraniferous minerals at Jaduguda, India. The overall geometric mean of the 222Rn flux from low-grade uranium (U) mill tailings being 1.19 Bq m-2 s-1, requisite attenuation of 37.8% to the recommended limit of 0.74 Bq m-2 s-1 can be attained by a typical clay coverage of 20 cm. Theoretically, feasible options for selection of overburden materials for stabilisation of the tailings piles are examined. Extrapolation studies with a turbulent diffusion model yield an atmospheric dispersion pattern of 222Rn in reasonable correspondence with the prevailing concentrations, especially for locations beyond a 4 km radius. The overall 222Rn contribution of the U complex to the ambient air is the same as that contributed by the adjoining land mass of 2.75 km radius in this mineralised terrain.     

GIS-based assessment and characterization of groundwater quality in a hard-rock hilly terrain of Western India

The growing population, pollution, and misuse of freshwater worldwide necessitate developing innovative methods and efficient strategies to protect vital groundwater resources. This need becomes more critical for arid/semi-arid regions of the world. The present study focuses on a GIS-based assessment and characterization of groundwater quality in a semi-arid hard-rock terrain of Rajasthan, western India using long-term and multi-site post-monsoon groundwater quality data. Spatio-temporal variations of water quality parameters in the study area were analyzed by GIS techniques. Groundwater quality was evaluated based on a GIS-based Groundwater Quality Index (GWQI). A Potential GWQI map was also generated for the study area following the Optimum Index Factor concept. The most-influential water quality parameters were identified by performing a map removal sensitivity analysis among the groundwater quality parameters. Mean annual concentration maps revealed that hardness is the only parameter that exceeds its maximum permissible limit for drinking water. GIS analysis revealed that sulfate and nitrate ions exhibit the highest (CV?>?30%) temporal variation, but groundwater pH is stable. Hardness, EC, TDS, and magnesium govern the spatial pattern of the GWQI map. The groundwater quality of the study area is generally suitable for drinking and irrigation (median GWQI?>?74). The GWQI map indicated that relatively high-quality groundwater exists in northwest and southeast portions of the study area. The groundwater quality parameter group of Ca, Cl, and pH were found to have the maximum value (6.44) of Optimum Index factor. It is concluded that Ca, Cl, and pH are three prominent parameters for cost-effective and long-term water quality monitoring in the study area. Hardness, Na, and SO₄, being the most-sensitive water quality parameters, need to be monitored regularly and more precisely.     

Field and GIS based post-tsunami assessment of Scleractinian coral cover in the Aerial Bay group of Islands, North Andaman, India

The Aerial Bay group of Islands are one of the diverse environments of Andaman & Nicobar Islands, where the coral reefs degraded much due to the natural calamity of tsunami on 26 December 2004. After this event, the entire North Andaman Islands got elevated, which resulted in the exposure of coral reefs during low tide, causing mass mortalities and destructions to this pristine environment. In order to understand the current status, bio-physical monitoring of coral reefs was carried out and compared with classified coral map of pre-tsunami period. A decline from 411.14 to 68.25 hectares (ha) of live coral area was observed in the Aerial Bay group of Islands. The dead corals and other abiotic factors (sand, mud and rubble) were observed to be 317.33 and 25.56 ha respectively, based on comparisons between ground truthed and classified pre-tsunami coral map (2004) processed in ArcGIS®. The detrended correspondence analysis of coral life form categories showed maximum cover of dead coral with algae, in comparison with the live corals. Bray-curtis cluster analysis revealed three different groups of study sites with 60 % similarity based on life-form categories within the coral reef environment.     

Leaching studies for tin recovery from waste e-scrap

Printed circuit boards (PCBs) are the most essential components of all electrical and electronic equipments, which contain noteworthy quantity of metals, some of which are toxic to life and all of which are valuable resources. Therefore, recycling of PCBs is necessary for the safe disposal/utilization of these metals. Present paper is a part of developing Indo-Korean recycling technique consists of organic swelling pre-treatment technique for the liberation of thin layer of metallic sheet and the treatment of epoxy resin to remove/recover toxic soldering material. To optimize the parameters required for recovery of tin from waste PCBs, initially the bench scale studies were carried out using fresh solder (containing 52.6% Sn and 47.3% Pb) varying the acid concentration, temperature, mixing time and pulp density. The experimental data indicate that 95.79% of tin was leached out from solder material using 5.5M HCl at fixed pulp density 50g/L and temperature 90°C in mixing time 165min. Kinetic studies followed the chemical reaction controlled dense constant size cylindrical particles with activation energy of 117.68kJ/mol. However, 97.79% of tin was found to be leached out from solder materials of liberated swelled epoxy resin using 4.5M HCl at 90°C, mixing time 60min and pulp density 50g/L. From the leach liquor of solder materials of epoxy resin, the precipitate of sodium stannate as value added product was obtained at pH 1.9. The Pb from the leach residue was removed by using 0.1M nitric acid at 90°C in mixing time 45min and pulp density 10g/L. The metal free epoxy resin could be disposed-of safely/used as filling material without affecting the environment.