Metal contamination in water, sediment and biota from a semi-enclosed coastal area

This study identifies and quantifies the spatial variations of metal contamination in water, sediment and biota: the common cockle (Cerastoderma edule) and the Mermaid’s glove sponge (Haliclona oculata), within a heavily anthropogenically impacted semi-enclosed estuarine–coastal area with a low ability to disperse and flush contaminants (Poole Harbour, UK). The results showed that metal contamination was detected in all environmental compartments. Water was polluted with As, and Hg sediment metals were mostly within “the possible effect range” in which adverse effects occasionally occurs. Cockles had considerable concentrations of Ni, Ag and Hg in areas close to pollution sources, and sponges accumulate Cu and Zn with very high magnitude. A systematic monitoring approach that includes biological monitoring techniques, which covers all embayments, is needed, and an integrated management of the semi-enclosed coastal zones should be based on the overall hydrological characteristics of these sensitive areas and their ability to self‐restore which is different than open coastal zones.     

Evaluation of long-term trends in hydrographic and nutrient parameters in a southeast US coastal river

The Nassau River estuary is located in northeast Florida adjacent to the eutrophic St. Johns River. Historically, development has been sparse in the Nassau River's catchment; thus, the system may provide a relatively undisturbed aquatic environment. To monitor the condition of the Nassau River estuary and to discern spatial and temporal trends in water quality, nutrients and hydrographic variables were assessed throughout the estuary from 1997 to 2011. Hydrographic (temperature, salinity, total suspended solids, and turbidity) and nutrient parameters (total phosphorus, ortho-PO₄ ^3?, total nitrogen, NH₄ ⁺, total Kjeldahl nitrogen, and NO₃ ^?) were monitored bimonthly at 12 sites in the mesohaline and polyhaline zones of the river. Nonparametric Kendall's Tau was implemented to analyze long-term water quality patterns. Salinity was found to increase with time, particularly in the mesohaline sampling sites. Dissolved oxygen decreased over time in the estuary and hypoxic conditions became increasingly frequent in the final years of the study. Nutrients increased in the estuary, ranging from 149 to 401 %. Rainfall data collected in adjacent conservation areas did not correlate well with nutrients as compared with stream discharge data collected in the basin headwaters, outside of the conservation lands, attributed here to expanding urbanization. During the study period, the Nassau basin underwent rapid human population growth and land development resulting in commensurate impacts to water quality. Nutrient and physical data collected during this study indicate that the Nassau River estuary is becoming more eutrophic with time.     

Seasonal stresses shift optimal intertidal algal habitats

We studied how the growth, reproduction, and survival of a common intertidal rockweed (Fucus distichus) varied across its tidal elevation at 14 sites around San Juan Island, Washington, USA in spring–summer and fall-winter seasons. We also measured a suite of environmental factors including temperature, light, emersion time, slope, fetch, and herbivory. To interpret the response of Fucus we included measurements of phlorotannins and carbon storage compounds (mannitol, laminarin). Growth and reproduction exhibited parallel patterns across tidal zones and sites. Tidal zone was a significant source of variation for many Fucus response variables, whereas variation between sites was high but not generally a significant factor explaining Fucus growth and physiology. Unexpectedly, the tidal zone in which Fucus achieved its highest growth and reproduction switched between seasons. High zone thalli grew and reproduced better than Mid zone thalli in fall but not in spring. This result can be explained by different combinations of factors influencing Fucus in each season. In spring, longer emersion times due to daytime low tides resulted in lower growth rates higher on the shore, likely due to carbon limitation. In fall during nighttime low tides, emersion and carbon limitation stresses were minimal. Overall, fall growth was lower than spring growth, but low fall light was not responsible. Instead, warmer average fall temperatures in the High zone apparently favored growth and reproduction relative to the Mid zone. In contrast, Mid zone thalli were subjected to more intense herbivory and hydrodynamic stress associated with wave exposure and steep substrata during the fall. At least for some seaweeds, living in the presumably more stressful high zone can actually confer higher integrated performance.     

Microbial activity and carbon, nitrogen, and phosphorus content in a subtropical seagrass estuary (Florida Bay): evidence for limited bacterial use of seagrass production

Bacterial abundance, production, and extracellular enzyme activity were determined in the shallow water column, in the epiphytic community of Thalassia testudinum, and at the sediment surface along with total carbon, nitrogen, and phosphorus in Florida Bay, a subtropical seagrass estuary. Data were statistically reduced by principle components analysis (PCA) and multidimensional scaling and related to T. testudinum leaf total phosphorus content and phytoplankton biomass. Each zone (i.e., pelagic, epiphytic, and surface sediment community) was significantly dissimilar to each other (Global R = 0.65). Pelagic aminopeptidase and sum of carbon hydrolytic enzyme (esterase, peptidase, and α- and β-glucosidase) activities ranged from 8 to 284 mg N m⁻² day⁻¹ and 113–1,671 mg C m⁻² day⁻¹, respectively, and were 1–3 orders of magnitude higher than epiphytic and sediment surface activities. Due to the phosphorus-limited nature of Florida Bay, alkaline phosphatase activity was similar between pelagic (51–710 mg P m⁻² day⁻¹) and sediment (77–224 mg P m⁻² day⁻¹) zones but lower in the epiphytes (1.1–5.2 mg P m⁻² day⁻¹). Total (and/or organic) C (111–311 g C m⁻²), N (9.4–27.2 g N m⁻²), and P (212–1,623 mg P m⁻²) content were the highest in the sediment surface and typically the lowest in the seagrass epiphytes, ranging from 0.6 to 8.7 g C m⁻², 0.02–0.99 g N m⁻², and 0.5–43.5 mg P m⁻². Unlike nutrient content and enzyme activities, bacterial production was highest in the epiphytes (8.0–235.1 mg C m⁻² day⁻¹) and sediment surface (11.5–233.2 mg C m⁻² day⁻¹) and low in the water column (1.6–85.6 mg C m⁻² day⁻¹). At an assumed 50% bacterial growth efficiency, for example, extracellular enzyme hydrolysis could supply 1.8 and 69% of epiphytic and sediment bacteria carbon demand, respectively, while pelagic bacteria could fulfill their carbon demand completely by enzyme-hydrolyzable organic matter. Similarly, previously measured T. testudinum extracellular photosynthetic carbon exudation rates could not satisfy epiphytic and sediment surface bacterial carbon demand, suggesting that epiphytic algae and microphytobenthos might provide usable substrates to support high benthic bacterial production rates. PCA revealed that T. testudinum nutrient content was related positively to epiphytic nutrient content and carbon hydrolase activity in the sediment, but unrelated to pelagic variables. Phytoplankton biomass correlated positively with all pelagic components and sediment aminopeptidase activity but negatively with epiphytic alkaline phosphatase activity. In conclusion, seagrass production and nutrient content was unrelated to pelagic bacteria activity, but did influence extracellular enzyme hydrolysis at the sediment surface and in the epiphytes. This study suggests that seagrass-derived organic matter is of secondary importance in Florida Bay and that bacteria rely primarily on algal/cyanobacteria production. Pelagic bacteria seem coupled to phytoplankton, while the benthic community appears supported by epiphytic and/or microphytobenthos production.     

A weed risk assessment model for use as a biosecurity tool evaluating plant introductions

New plant taxa from around the world continue to be imported into Australia and New Zealand. Many of these taxa have the potential to become agricultural or environmental weeds and this risk needs to be assessed before allowing their entry. A weed risk assessment system is described that uses information on a taxon's current weed status in other parts of the world, climate and environmental preferences, and biological attributes. The system is designed to be operated by quarantine personnel via a user-friendly computer interface.The model was tested against experts' scores for weediness for 370 taxa present in Australia, representing both weeds and useful taxa from agriculture, the environment, and other sectors. The model was judged on its ability to correctly ‘reject’ weeds, ‘accept’ non-weeds, and generate a low proportion of taxa which could not be decisively categorised, termed ‘evaluate’. More than 70% of the taxa were rejected or accepted. All taxa classified as serious weeds, and most minor weeds, were rejected or required further evaluation, while only 7% of non-weeds were rejected. The model was modified to New Zealand conditions and evaluated against the opinions of several groups of experts and against economic measures. The model produced a weediness score very similar to the mean of the experts scores. The latter were highly variable: agriculturalists tended to accept known weeds, conservationists tended to reject most adventive taxa, and only botanists produced scores similar to the model. The model scores also tended to be independent of economic value as measured in this study. The model could be adapted for use as a screening tool in any region of the world.     

Motor Vehicle Crashes on Bridges and Countermeasure Opportunities

There are approximately 600,000 highway bridges in the United States. These structures differ from surface streets and highways in terms of their physical properties and operational characteristics, which in turn, affect the risk of motor-vehicle crashes and the kinds of crashes that occur. The purpose of this study was to identify motor-vehicle crash patterns and contributing factors to bridge crashes and related countermeasure opportunities on four urban bridges in the New York metropolitan area. A crash typology was developed using the narratives and diagrams in police crash reports in addition to standard police classifications of crashes. The final dataset contained records of 1,381 police-reported crashes. The four bridges studied had higher crash rates than their respective approach roads. Four major crash types accounted for approximately 90% of the bridge crashes. Primary collision factors are reviewed, and potential countermeasures are discussed.     

A cultural practice of drinking realgar wine leading to elevated urinary arsenic and its potential health risk

Toasting friends and family with realgar wines and painting children's foreheads and limbs with the leftover realgar/alcohol slurries is an important customary ritual during the Dragon Boat Festival (DBF); a Chinese national holiday and ancient feast day celebrated throughout Asia. Realgar is an arsenic sulfide mineral, and source of highly toxic inorganic arsenic. Despite the long history of realgar use during the DBF, associated risk to human health by arsenic ingestion or percutaneous adsorption is unknown. To address this urine samples were collected from a cohort of volunteers who were partaking in the DBF festivities. The total concentration of arsenic in the wine consumed was 70 mg L?1 with all the arsenic found to be inorganic. Total arsenic concentrations in adult urine reached a maximum of ca. 550 μg L?1 (mean 220.2 μg L?1) after 16 h post-ingestion of realgar wine, while face painting caused arsenic levels in children's urine to soar to 100 μg L?1 (mean 85.3 μg L?1) 40 h after the initial paint application. The average concentration of inorganic arsenic in the urine of realgar wine drinkers on average doubled 16 h after drinking, although this was not permanent and levels subsided after 28 h. As would be expected in young children, the proportions of organic arsenic in the urine remained high throughout the 88-h monitoring period. However, even when arsenic concentrations in the urine peaked at 40 h after paint application, concentrations in the urine only declined slightly thereafter, suggesting pronounced longer term dermal accumulation and penetration of arsenic. Drinking wines blended with realgar or using realgar based paints on children does result in the significant absorption of arsenic and therefore presents a potentially serious and currently unquantified health risk.     

Interactive effects of cadmium and hypoxia on metabolic responses and bacterial loads of eastern oysters Crassostrea virginica Gmelin

Pollution by toxic metals including cadmium (Cd) and hypoxia are important stressors in estuaries and coastal waters which may interactively affect sessile benthic organisms, such as oysters. We studied metabolic responses to prolonged hypoxic acclimation (2 weeks at 5% O₂) in control and Cd-exposed (30 d at 50 μg L⁻¹ Cd) oysters Crassostrea virginica, and analyzed the effects of these stressors on abundance of Vibrio spp. in oysters. Hypoxia-acclimated oysters retained normal standard metabolic rates (SMR) at 5% O₂, in contrast to a decline of SMR observed during acute hypoxia. However, oysters spent more time actively ventilating in hypoxia than normoxia resulting in enhanced Cd uptake and 2.7-fold higher tissue Cd burdens in hypoxia. Cd exposure led to a significant decrease in tissue glycogen stores, increase in free glucose levels and elevated activity of glycolytic enzymes (hexokinase and aldolase) indicating a greater dependence on carbohydrate catabolism. A compensatory increase in activities of two key mitochondrial enzymes (citrate synthase and cytochrome c oxidase) was found during prolonged hypoxia in control oysters but suppressed in Cd-exposed ones. Cd exposure also resulted in a significant increase in abundance of Vibrio parahaemolyticus and Vibrio vulnificus levels during normoxia and hypoxia, respectively. Overall, Cd- and hypoxia-induced changes in metabolic profile, Cd accumulation and bacterial flora of oysters indicate that these stressors can synergistically impact energy homeostasis, performance and survival of oysters in polluted estuaries and have significant consequences for transfer of Cd and bacterial pathogens to the higher levels of the food chain.     

Inorganic arsenic and trace elements in Ghanaian grain staples

A total of 549 samples of rice, maize, wheat, sorghum and millet were obtained from markets in Ghana, the EU, US and Asia. Analysis of the samples, originating from 21 countries in 5 continents, helped to establish global mean trace element concentrations in grains; thus placing the Ghanaian data within a global context. Ghanaian rice was generally low in potentially toxic elements, but high in essential nutrient elements. Arsenic concentrations in rice from US (0.22 mg/kg) and Thailand (0.15 mg/kg) were higher than in Ghanaian rice (0.11 mg/kg). Percentage inorganic arsenic content of the latter (83%) was, however, higher than for US (42%) and Thai rice (67%). Total arsenic concentration in Ghanaian maize, sorghum and millet samples (0.01 mg/kg) was an order of magnitude lower than in Ghanaian rice, indicating that a shift from rice-centric to multigrain diets could help reduce health risks posed by dietary exposure to inorganic As.