An evaluation of procedures for the digestion of soils and vegetation from areas with metalliferous pollution

Various digestion or extraction processes are available for the determination of trace metals in environmental samples using diverse analytical techniques. Our research examines the most efficient and reproducible methods of digestion utilising readily available laboratory equipment, reagents and instrumentation. Extraction procedures reported in over 80 recent research works were considered and the most common methods were critically evaluated. Thus nitric acid, nitric acid/perchloric acid, aqua regia, nitric acid/hydrogen peroxide and microwave-assisted methods were investigated using soil and leaf samples collected adjacent to three old metalliferous workings. Analysis was carried out using Flame Atomic Absorption Spectrometry and the results were evaluated. Differences in efficiency of extraction relative to specific metals and different substrates are highlighted and recommendations made for a digestion procedure suitable for environmental samples. Aqua regia produced the most accurate, efficient and reproducible results; we consider methods of improving both of these factors and sample preparation. Our recommended methodology has applications in environmental surveys, environmental geology, environmental toxicology, geochemistry and provides a means of obtaining meaningful and reliable results for the determination of metals present in soils, minerals and vegetation.     

Prenatal diagnosis of Pompe's disease (type ii glycogenosis) in chorionic villus biopsy using maltose as a substrate

Uncultured trophoblasts obtained from chorionic villus biopsy during the gestation period of 8–12 weeks were assayed for alpha-glucosidase activity using maltose as the substrate. Only one major form of maltase activity with a pH optimum at 4.0 was demonstrated. Using this method, we performed prenatal diagnosis on three pregnancies at risk for the infantile form of type II glycogen storage disease. Two affected fetuses and one unaffected fetus were predicted and the diagnosis was subsequently confirmed. The maltose assay offered a direct, simple, and sensitive method for prenatal diagnosis of Pompe's disease in the first trimester.     

MM5 simulations for air quality modeling: An application to a coastal area with complex terrain

A series of modifications were implemented in MM5 simulation in order to account for wind along the Santa Clarita valley, a north–south running valley located in the north of Los Angeles. Due to high range mountains in the north and the east of the Los Angeles Air Basin, sea breeze entering Los Angeles exits into two directions. One branch moves toward the eastern part of the basin and the other to the north toward the Santa Clarita valley. However, the northward flow has not been examined thoroughly nor simulated successfully in the previous studies. In the present study, we proposed four modifications to trigger the flow separation. They were (1) increasing drag over the ocean, (2) increasing soil moisture content, (3) selective observational nudging, and (4) one-way nesting for the innermost domain. The Control run overpredicted near-surface wind speed over the ocean and sensible heat flux, in an urbanized area, which justifies the above 1st and 2nd modification. The Modified run provided an improvement in near-surface temperature, sensible heat flux and wind fields including southeasterly flow along the Santa Clarita valley. The improved MM5 wind field triggered a transport to the Santa Clarita valley generating a plume elongated from an urban center to the north, which did not exist in MM5 Control run. In all, the modified MM5 fields yielded better agreement in both CO and O₃ simulations especially in the Santa Clarita area.     

Adverse perinatal outcome in patients screen-positive for neural tube defects and fetal down syndrome

An association between various abnormal mid-trimester maternal serum analyte values and adverse perinatal outcome has been reported. From an original sample of 14 857 women, we observed five women who were ‘screen-positive’ for both neural tube defects [maternal serum alpha-fetoprotein (MSAFP) ≥2·5 multiples of the median] and Down syndrome [risk ≥1/274 using MSAFP, maternal serum unconjugated oestriol (MSuE3), maternal serum human chorionic gonadotropin (MShCG), and maternal age]. The four patients who elected to undergo amniocentesis all demonstrated both normal karyotype and normal amniotic fluid AFP levels. All five cases were associated with intrauterine growth retardation (IUGR) and abnormal pregnancy outcomes. Two cases exhibiting severe IUGR on ultrasound examination were terminated at 19·1 and 21·2 weeks, respectively; the former also exhibited fetal calcifications and positive maternal serology for toxoplasmosis. In another case, fetal demise occurred at 36 weeks' gestation in a patient who had been treated for syphilis in the second trimester. Neither infection was confirmed in fetal tissue studies. Though resulting in live births, the remaining two cases required operative deliveries; emergency Caesarean sections for fetal distress were performed at 38 and 32 weeks, respectively, the latter case being associated with severe pre-eclampsia. We conclude that elevated mid-trimester MSAFP levels concurrent with maternal serum analyte values associated with increased risk for fetal Down syndrome may presage a poor perinatal outcome, particularly IUGR and possibly congenital infection.     

The effects of oil spill and clean-up on dominant US Gulf coast marsh macrophytes: a review

The objective of this review was to synthesize existing information regarding the effects of petroleum hydrocarbons on marsh macrophytes in a manner that will help guide research and improve spill-response efficiency. Petroleum hydrocarbons affect plants chemically and physically. Although plants sometime survive fouling by producing new leaves, even relatively non-toxic oils can stress or kill plants if oil physically prevents plant gas-exchange. Plant sensitivity to fouling varies among species and among populations within a species, age of the plant, and season of spill. Physical disturbance and compaction of vegetation and soil associated with clean-up activities following an oil spill appear to have detrimental effects on the US Gulf coast marshes. Other techniques, including the use of chemicals such as cleaners or bioremediation, may be necessary to address the problem. Clean-up may also be beneficial when timely removal prevents oil from migrating to more sensitive habitats.     

Distributed process modeling for regional assessment of coastal vulnerability to sea-level rise

Sea-level rise involves increases in the coastal processes of inundation and erosion which are affected by a complex interplay of physical environmental parameters at the coast. Many assessments of coastal vulnerability to sea-level rise have been detailed and localised in extent. There is a need for regional assessment techniques which identify areas vulnerable to sea-level rise. Four physical environmental parameters – elevation, exposure, aspect and slope, are modeled on a regional scale for the Northern Spencer Gulf (NSG) study area using commonly available low-resolution elevation data of 10 m contour interval and GIS-based spatial modeling techniques. For comparison, the same parameters are modeled on a fine-scale for the False Bay area within the NSG using high-resolution elevation data. Physical environmental parameters on the two scales are statistically compared to coastal vulnerability classes as identified by Harvey et al. [1] using the Spearman rank-correlation test and stepwise linear regression. Coastal vulnerability is strongly correlated with elevation and exposure at both scales and this relationship is only slightly stronger for the high resolution False Bay data. The results of this study suggest that regional scale distributed coastal process modeling may be suitable as a first cut in assessing coastal vulnerability to sea-level rise in tide-dominated, sedimentary coastal regions. Distributed coastal process modeling provides a suitable basis for the assessment of coastal vulnerability to sea-level rise of sufficient accuracy for on-ground management and priority-setting on a regional scale.     

Marine protected areas and local coastal conservation and management in Hong Kong

Abstract

This paper presents as case studies some New Zealand local government sustainable development initiatives in cleaner production and civic building design. From a series of structured interviews with key players, it describes key motivators and demotivators and puts these in the context of behaviour change theory. These enable a set of actions for local government to be developed that, if applied, could result in an advancement of the sustainable development agenda. However, other critical factors are given that are external to these actions and on which their overall success is dependent. In reviewing these factors as a group we conclude that far greater understanding of the partnership formation process and its dynamic in engendering successful sustainable development decision-making processes is crucial to move beyond the rhetoric of partnerships and enter the more complex and difficult world of truly participatory approaches.     

Social learning and sustainable communities: An interim assessment of research into sustainable communities projects in the UK

Abstract

The delivery of many of the most pressing environmental issues will rely on changes in environmental attitudes and behaviour at community level. At a UN Special Session in 1997, the British Government highlighted its initiatives on Local Agenda 21 (LA21) and Going for Green (GFG) as significant advances. This paper adds a new perspective, drawing on the range of experiences of some of the research teams that have been working with local authorities on pilot Sustainable Community Projects (SCPs) in England and Scotland. It sheds light on three substantive themes: the tensions inherent in the implementation of internationally and nationally agreed goals through local action; the ambiguity of local agencies acting as facilitators of community ownership of processes, and the requirements for successful partnership between local authorities and higher education.     

Glutathione and associated enzymes in toxic cataractogenesis-selenite model

Glutathione, gamma-glutamyl cysteine synthetase (gamma -GCS) and glutathione reductase (GSH-R) activity were determined biochemically in the lens during various stages after subcutaneous administration of sodium selenite in multiple low dosages and single high dosages. The GSH concentration and gamma-GCS and GSH-R activity declined progressively after the selenite administration. The changes observed were discussed in relation to the possible role of selenite interaction with GSH and the enzymes.