Increased binding of autoantibody to erythrocytes treated with cigarette smoke extract

When human erythrocytes were incubated with cigarette smoke (CS) exract for 1 h, the cells were oxidized as assessed by the decrease of cytoplasmic glutathione level. Because the ability of the CS extract pretreated with antioxidants and enzymes that destroy superoxide and hydrogen peroxide was lower, various oxidants in the extract may be responsible for the reduction of cytoplasmic glutathione levels. The binding of autologous IgG to the CS extract-treated cells as assessed by enzyme immunoassay was dramatically increased. Autologous IgG responsible for the binding may be anti-band 3 autoantibody, because inhibitors of anti-band 3 autoantibody binding effectively inhibited the IgG binding. The results indicate that oxidants in CS extract rendered erythrocytes susceptible to binding of anti-band 3 autoantibody, a known antibody that binds to senescent erythrocytes.     

Integrating information for better environmental decisions

As more is learned about the complex nature and extent of environmental impacts from progressive human disturbance, scientists, policy analysts, decision makers, educators, and communicators are increasingly joining forces to develop strategies for preserving and protecting the environment. The Eco-Informa Foundation is an educational scientific organization dedicated to promoting the collaborative development and sharing of scientific information. The Foundation participated in a recent international conference on environmental informatics through a special symposium on integrating information for better environmental decisions. Presentations focused on four general themes: (1) remote sensing and data interpretation, including through new knowledge management tools; (2) risk assessment and communication, including for radioactively contaminated facilities, introduced biological hazards, and food safety; (3) community involvement in cleanup projects; and (4) environmental education. The general context for related issues, methods and applications, and results and recommendations from those discussions are highlighted here.     

An evaluation of woodland reclamation on strip-mined lands in east Texas

We compared the composition and structural characteristics of reclaimed and native woody plant communities near Fairfield, Texas, to evaluate the effectiveness of woodland reclamation 3–11 years since establishment. Species composition, foliage density, canopy cover, and woody plant densities were recorded in plots randomly placed along transects bisecting blocks of reclaimed and native woodlands. During summer, vertical foliage densities at heights ≤2 m were similar among native and reclaimed areas. Foliage density and canopy cover declined in reclaimed blocks during winter, but remained relatively constant in native woodlands, where evergreens and vines were more common. Canopy cover was absent in reclaimed woodlands <6 years old but increased with age in 6 to 11-year-old blocks. These data indicated that approximately 27 years will be needed before trees in reclaimed blocks will achieve the stature of canopy trees in native woodlands. Reclaimed woodlands contained different woody plant species and had lower woody stem densities compared to native woodlands. On average, stem densities in reclaimed blocks were six times lower than densities in native woodlands. Comparisons with planting records indicate that survival of most commonly planted woody species was low. Only green ash(Fraxinus pennsylvanica), Russian oliver(Elaeagnus commutata), smooth sumac(Rhus glabra), and redbud(Cercis canadensis) had estimated survival rates >50%. Reclamation procedures used at Big Brown Mine (BBM) during 1981–1988 have not produced woodland habitats with vegetative characteristics comparable to premined woodlands and may not be providing the cover needed to encourage use by certain wildlife species. Procedures for improving woodland reclamation are recommended.     

Biota Connect Aquatic Habitats throughout Freshwater Ecosystem Mosaics

Freshwater ecosystems are linked at various spatial and temporal scales by movements of biota adapted to life in water. We review the literature on movements of aquatic organisms that connect different types of freshwater habitats, focusing on linkages from streams and wetlands to downstream waters. Here, streams, wetlands, rivers, lakes, ponds, and other freshwater habitats are viewed as dynamic freshwater ecosystem mosaics (FEMs) that collectively provide the resources needed to sustain aquatic life. Based on existing evidence, it is clear that biotic linkages throughout FEMs have important consequences for biological integrity and biodiversity. All aquatic organisms move within and among FEM components, but differ in the mode, frequency, distance, and timing of their movements. These movements allow biota to recolonize habitats, avoid inbreeding, escape stressors, locate mates, and acquire resources. Cumulatively, these individual movements connect populations within and among FEMs and contribute to local and regional diversity, resilience to disturbance, and persistence of aquatic species in the face of environmental change. Thus, the biological connections established by movement of biota among streams, wetlands, and downstream waters are critical to the ecological integrity of these systems. Future research will help advance our understanding of the movements that link FEMs and their cumulative effects on downstream waters.     

Effect of arsenic contaminated irrigation water on Lens culinaris L. and toxicity assessment using lux marked biosensor

Contamination of irrigation water represents a major constraint to Bangladesh agriculture, resulting in elevated levels in the terrestrial systems. Lux bacterial biosensor technology has previously been used to measure the toxicity of metals in various environmental matrices. While arbuscular mycorrhizal fungi have their most significant effect on phosphorus uptake, but showed alleviated metal toxicity to the host plant. The study examined the effects of arsenic and inoculation with an arbuscular mycorrhizal fungus, Glomus mosseae, on lentil (Lens culinaris L. cv. Titore). Plants were grown with and without arbuscular mycorrhizal inoculum for 9 weeks in a sand and terra-green mixture (50:50, V/V) and watered with five levels of arsenic (0, 1, 2, 5, 10 mg As/L arsenate). The results showed that arsenic addition above 1 mg/L significantly reduced percentage of mycorrhizal root infection. On further analysis a close relationship was established with the vegetative and reproductive properties of lentil (L. culinaris) plants compared to the percentage bioluminescence of the soil leachate. However, arbuscular mycorrhizal fungal inoculation reduced arsenic concentration in roots and shoots. Higher concentrations of arsenic (5, 10 mg As/L arsenate) reduced the mycorrhizal efficiency to increase phosphorus content and nitrogen fixation. Therefore, this study showed that increased concentration of arsenic in irrigation water had direct implications to the lentil (L. culinaris) plants overall performance. Moreover the use of bioassay demonstrated that mycorrhiza and clay particle reduced arsenic bioavailability in soil.     

Making scents of defense: do fecal shields and herbivore-caused volatiles match host plant chemical profiles?

Many plant families have aromatic species that produce volatile compounds which they release when damaged, particularly after suffering herbivory. Monarda fistulosa (Lamiaceae) makes and stores volatile essential oils in peltate glandular trichomes on leaf and floral surfaces. This study examined the larvae of a specialist tortoise beetle, Physonota unipunctata, which feed on two M. fistulosa chemotypes and incorporate host compounds into fecal shields, structures related to defense. Comparisons of shield and host leaf chemistry showed differences between chemotypes and structures (leaves vs. shields). Thymol chemotype leaves and shields contained more of all compounds that differed than did carvacrol chemotypes, except for carvacrol. Shields had lower levels of most of the more volatile chemicals than leaves, but more than twice the amounts of the phenolic monoterpenes thymol and carvacrol and greater totals. Additional experiments measured the volatiles emitted from M. fistulosa in the absence and presence of P. unipunctata larvae and compared the flower and foliage chemistry of plants from these experiments. Flowers contained lower or equal amounts of most compounds and half the total amount, compared to leaves. Plants subjected to herbivory emitted higher levels of most volatiles and 12 times the total amount, versus controls with no larvae, including proportionally more of the low boiling point chemicals. Thus, chemical profiles of shields and volatile emissions are influenced by the amounts and volatilities of compounds present in the host plant. The implications of these results are explored for the chemical ecology of both the plant and the insect.     

One day is all it takes: circadian modulation of the retrieval of colour memories in honeybees

In this study, we examined how honeybees coped with successive tasks of colour discrimination with conflicting demands. Free-flying honeybees (Apis mellifera) were trained on tasks in which they had to choose one of three colours to obtain a reward of sugar water. In acquisition, the bees learned this task in about four trials of training. Colour memory was retained after 24-h delay in an unrewarded retention test. Integration experiments were then conducted in which the bees had to learn two successive tasks of colour discrimination with conflicting demands, task 1 for 20 trials and task 2 for ten trials. In task 1, one of three colours provided sugar water while the other two provided tap water, while in task 2 a different colour provided the reward. The bees were given unrewarded tests immediately after training on task 2 and then re-tested after 10 min, 22 h (circadian time of the start of task 1 training), or 24 h (circadian time of the end of task 2 training). Bees strongly preferred the rewarded colour for task 2 on immediate testing and after 10-min delay. After 22-h delay, they switched their preference to the rewarded colour for task 1. But after 24-h delay, the bees again strongly preferred the rewarded colour for task 2. Further tests at a number of delays between 0 and 22 h revealed a sigmoidal pattern of rise in the preference for the task 1 colour. We conclude that circadian time modulates the retrieval of colour memories in honeybees, even when all the training took place in a single day.