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981.
Delta-aminolevulinate dehydratase (ALAD) activity has been used in prokaryotes and eukaryotes as a biomarker for environmental
lead (Pb) exposure and toxicity. Microorganisms are sensitive indicators of toxicity at the fundamental level of ecological
organization, but bacterial biomarker studies are focused on the Pseudomonas strains in Group I and E coli. The objectives of the present work were to determine if Burkholderia gladioli belonging to group II, due to its 16SrRNA similarity, can be used as biomarker in metal contamination and compare its possible
usage with Pseudomonas aeruginosa and Citrobacter freundii (previously known as Esherichia freundii) and Bacterium freundii which are classified in Group I. In this study, ALAD activity in an environmental strains of Burkholderia gladioli, Pseudomonas aeruginosa, Citrobacter freundii were investigated to evaluate potential inhibition by Pb and other toxic metals. When the ALAD activity of Burkholderia gladioli was tested, Co and Pb decreased activity by 27 and 71%, respectively. In addition to these findings, Zn increased the activity
up to 26%. These effects were found to be statistically meaningful (p < 0.05). It was determined that the increase of lead concentration inhibites the ALAD activity at each of the three strains.
There was a statistically significant dose–response relationship between ALAD activity in cells of Burkholderia gladioli and Pb (Pearson correlation coefficent = −0.665; r
2 = 0.665, and p < 0.001). The strongest ALAD inhibition which was measured was 90% at Burkholderia gladioli when protein extracts were incubated with 750 μM of Pb. The relationship between Pb and ALAD activity was statistically described
by [ALAD Activity] = 0.476−0.000597 × [Pb]. According to the obtained results, we suggest that the ALAD of Burkholderia gladioli can be used as a biomarker for lead contamination in the environment. 相似文献
982.
Yu Y Wen S Lü H Feng Y Wang X Sheng G Fu J 《Environmental monitoring and assessment》2008,137(1-3):275-285
The diurnal variation of atmospheric carbonyls and VOCs in a forest in south China were studied in summer 2004. Twenty kinds
of carbonyls and eight kinds of VOCs were identified and quantified. Formaldehyde and acetaldehyde were the two most abundant
carbonyls, while the most abundant VOCs were isoprene, followed by o-xylene. Most C3-C10 carbonyls had higher concentrations from 09:00 to 15:00, and their levels were lower during night-time and often reached
the lowest in early morning. Formaldehyde and acetaldehyde, however, showed two high levels in their diurnal patterns partly
due to their different sources and sinks. The VOCs had different diurnal patterns compared to most carbonyls. The highest
concentrations were observed from 03:00 to 06:00 for 1-butene, from 06:00 to 12:00 for isoprene, and from 12:00 to 15:00 for
α-pinene. The highest levels for aromatic hydrocarbons occurred during midnight and the lowest in late afternoon. According
to the study, emissions from vegetation and photo-oxidation of gas-phase hydrocarbons were the main sources for some carbonyls
and VOCs in this region. Other compounds, such as formaldehyde, acetaldehyde and BTEX, showed anthropogenic sources. 相似文献
983.
We present a new system for microscopic multicolour variable chlorophyll fluorescence imaging of aquatic phototrophs. The
system is compact and portable and enables microscopic imaging of photosynthetic performance of individual cells and chloroplasts
using different combinations of blue, green, red or white light. Automated sequential exposure of microscopic samples to the
three excitation colours enables subsequent deconvolution of the resulting fluorescence signals and colour marking of cells
with different photopigmentation, i.e., cyanobacteria, green algae, red algae and diatoms. The photosynthetic activity in
complex mixtures of phototrophs and natural samples can thus be assigned to different types of phototrophs, which can be quantified
simultaneously. Here, we describe the composition and performance of the new imaging system and present applications with
both natural phytoplankton and microalgal culture samples. 相似文献
984.
Jelle S. van Zweden Christoph Grüter Sam M. Jones Francis L.W. Ratnieks 《Behavioral ecology and sociobiology》2011,65(6):1277-1282
Social insects need to defend their nest against robbery, parasitism and predation. The stingless bee Tetragonisca angustula is unique in that it has guards that hover near the nest entrance in addition to guards that stand at the entrance. We tested
both the general hypothesis that hovering guards increase the effectiveness with which flying intruders are detected and the
specific hypothesis that hovering guards improve the detection of workers of the obligate robber bee, Lestrimellita limao. In an intraspecific study comparing colonies, we found a strong positive relationship between the number of hovering guards
and the distance at which a dummy robber bee or L. limao worker, experimentally moved towards the nest entrance, was detected. These results were mirrored in an interspecific study
showing that four species of stingless bees with similar population colonies but which lacked hovering guards, detected L. limao only at the nest entrance, in contrast to T. angustula. In addition, we found that a greater number of attacks by guards occurred when dummies were impregnated with citral, a major
component of L. limao mandibular gland odour. Our results support the hypothesis that T. angustula hovering guards increase the detection perimeter for flying intruders, especially L. limao. 相似文献
985.
Public signaling plays an important role in territorial and sexual displays in animals; however, in certain situations, it
is advantageous to keep signaling private to prevent eavesdropping by unintended receivers. In the northeastern Pacific, two
populations of killer whales (Orcinus orca), fish-eating “resident” killer whales and mammal-eating “transient” killer whales, share the same habitat. Previous studies
have shown that residents use whistles as private signals during close-range communication, where they probably serve to coordinate
behavioral interactions. Here, we investigated the whistling behavior of mammal-eating killer whales, and, based on divergent
social structures and social behaviors between residents and transients, we predicted to find differences in both whistle
usage and whistle parameters. Our results show that, like resident killer whales, transients produce both variable and stereotyped
whistles. However, clear differences in whistle parameters between ecotypes show that the whistle repertoire of mammal-eating
killer whales is clearly distinct from and less complex than that of fish-eating killer whales. Furthermore, mammal-eating
killer whales only produce whistles during “milling after kill” and “surface-active” behaviors, but are almost completely
silent during all other activities. Nonetheless, whistles of transient killer whales may still serve a role similar to that
of resident killer whales. Mammal-eating killer whales seem to be under strong selection to keep their communication private
from potential prey (whose hearing ranges overlap with that of killer whales), and they appear to accomplish this mainly by
restricting vocal activity rather than by changes in whistle parameters. 相似文献
986.
E. Petter Axelsson Joakim Hj?lt��n Thomas G. Whitham Riitta Julkunen-Tiitto Gilles Pilate Anders Wennstr?m 《Chemoecology》2011,21(3):161-169
Bioassays with a non-target slug (Deroceras spp.) and chemical analyses were conducted using leaf tissue from already existing genetically modified insect-resistant
aspen trees to examine whether genetic modifications to produce Bacillus thuringiensis (Bt) toxins could affect plant phytochemistry, which in turn might influence plant–herbivore interactions. Three major patterns
emerged. First, two independent modifications for Bt resistance affected the phytochemical profiles of leaves such that both
were different from the isogenic wild-type (Wt) control leaves, but also different from each other. Among the contributors
to these differences are substances with a presumed involvement in resistance, such as salicortin and soluble condensed tannins.
Second, bioassays with one Bt line suggest that the modification somehow affected innate resistance (“Innate” is used here
in opposition to the “acquired” Bt resistance) in ways such that slugs preferred Bt over Wt leaves. Third, the preference
test suggests that the innate resistance in Bt relative to Wt plants may not be uniformly expressed throughout the whole plant
and that leaf ontogeny interacts with the modification to affect resistance. This was manifested through an ontogenetic determined
increase in leaf consumption that was more than four times higher in Bt compared to Wt leaves. Our result are of principal
importance, as these indicate that genetic modifications can affect innate resistance and thus non-target herbivores in ways
that may have commercial and/or environmental consequences. The finding of a modification–ontogeny interaction effect on innate
resistance may be especially important in assessments of GM plants with a long lifespan such as trees. 相似文献
987.
Thomas Kneib Felix Knauer Helmut Küchenhoff 《Environmental and Ecological Statistics》2011,18(1):1-25
The investigation of animal habitat selection aims at the detection of selective usage of habitat types and the identification
of covariates influencing their selection. The results not only allow for a better understanding of the habitat selection
process but are also intended to help improve the conservation of animals. Usually, habitat selection by larger animals is
assessed by radio-tracking or visual observation studies, where the chosen habitat is determined for some animals at a set
of specific points in time. Hence the resulting data often have the following structure: a categorical variable indicating
the habitat type selected by an animal at a specific point in time is repeatedly observed and will be explained by covariates.
These may either describe properties of the habitat types currently available and/or properties of the animal. In this paper,
we present a general approach to the analysis of such data in a categorical regression setup. The proposed model generalizes
and improves upon several of the approaches previously discussed in the literature. In particular, it accounts for changing
habitat availability due to the movement of animals within the observation area. It incorporates both habitat- and animal-specific
covariates, and includes individual-specific random effects to account for correlations introduced by the repeated measurements
on single animals. Furthermore, the assumption that the effects are linear can be dropped by including the effects in nonparametric
manner based on a penalized spline approach. The methodology is implemented in a freely available software package. We demonstrate
the general applicability and the potential of the proposed approach in two case studies: The analysis of a songbird community
in South-America and a study on brown bears in Central Europe. 相似文献
988.
Colby J. Tanner Gül Deniz Salalι Andrew L. Jackson 《Behavioral ecology and sociobiology》2011,65(2):249-256
Human activity can have a large impact on surrounding ecosystems. For example, humans alter resource distributions for other
species, potentially modifying these species competitive dynamics. These changes in local competitive processes are frequently
associated with species invasions. Here, we investigate how differences in resource distribution affect competitive behaviour
using the highly invasive European shore crab (Carcinus maenas). Using a controlled laboratory experiment in combination with behaviour assays and social network analysis, we show that
individuals feeding in habitats with clumped food distributions are more aggressive than individuals feeding in habitats where
food is evenly dispersed, and this aggression is present even on days where crabs are not feeding. Additionally, this persistent
aggression can be induced, suggesting that individuals of this invasive species possess the flexibility to modify their competitive
behaviours in response to differences in food distributions. Furthermore, we show how these individual responses can lead
to changes in overall organisation of aggressive interactions within a population. We discuss these results in relation to
how human impacts can have long-term effects on competitive behavioural strategies, and how behavioural flexibility can allow
invasive species to colonise and persist in highly impacted sites such as urban ecosystems. 相似文献
989.
Across animal taxa, exclusive female offspring care has evolved repeatedly from biparental care, suggesting that the latter
becomes evolutionarily unstable under certain conditions. Both the attributes of a species and the environment it experiences
can help to predict shifts from one particular care mode to another. Nevertheless, factors inducing differences in care strategies
among closely related species, or seasonal variation within species, have been subject to surprisingly little empirical testing.
Here, we report the results of a field-based study that examined both among and within species variation in mate desertion
in five species of closely related Nicaraguan cichlid fish in the genera Amphilophus and Amatitlania. The results show a link between female body size and male involvement in offspring care. Specifically, the larger the species
the less often males were found to provide extended care. Furthermore, we found that solitary females became more common towards
the end of the breeding season. We discuss the implications of this finding in the context of previous theoretical and empirical
contributions regarding the frequency of offspring desertion by males. 相似文献
990.