Phenogenetic Analysis of Pygmy Wood Mouse (Apodemus uralensis Pall.) Populations in the Zone of the Eastern Ural Radioactive Trace (EURT)

Monitoring of A. uralensis populations in Kamenskii raion, Sverdlovsk oblast, in 1992–2002 and in Kaslinskii raion, Chelyabinsk oblast, in 2000–2001 revealed an increase in the proportion and diversity of minor morphogenetic aberrations and abnormalities in the structure of the cranium along the axis of the Eastern Ural Radioactive Trace (EURT). The samples from the southern and northern parts of the EURT (contaminated with ⁹⁰Sr to 500 and 4 Ci/km², respectively) were characterized by directed deviations from the control with respect to the frequencies of phenes of nonmetric cranial traits and an increase in the level of their fluctuating asymmetry in young females, which is indicative of epigenetic rearrangements in populations living in a radioactive environment.     

Residues of dicofol on cucumber grown under plastic covers in Jordan

Residues of dicofol were determined on cucumber leaves and fruits under plastic house (PH) and plastic tunnels (PT). Five sprays, 8 d apart, were applied at 0.15% concentration. Initial deposits on leaves were 48 and 58 ppm under PH and PT, respectively. In the last sampling date of leaves, the amounts of 191 and 135 ppm were detected under both cultures, respectively. There was a continuous increase in the initial residue after each spray. The highest amount of dicofol (401) was determined 1 d after the fifth spray under PH. The exposure to high residues may pose a risk to fieldworkers. On cucumber fruits, residues of 0.95 and 1.60 ppm were determined 1 d after the fourth spray under PH and PT, respectively. These residues decreased after 4 d to 0.40 and 1.49 ppm, respectively. Almost no detectable residues could be determined 8 d after sprays number 4 and 5 under both cultures. All dicofol residues on the fruits were below the tolerance level of 2 ppm.     

Morphological correlates of intraspecific grafting reactions in the marine demosponge Callyspongia diffusa

There are profound differences in the ways that the sponge Callyspongia diffusa reacts to self versus non-self tissue contact. Syngeneic or isogeneic contacts result in rapid tissue fusion and persistent maintenance of normal sponge structure. However, allogeneic contacts result in immune-type rejection reactions leading to tissue death at the interface. Using grafted sponge tissues, both types of response were subject to fine-structural analysis by scanning electron microscopy. Isografts showed no overt cellular reactions other than compatible fusion, whereas sponge tissues at allograft interfaces showed incompatibility reactions. The major steps in the histopathologic sequence of events in allograft reactions may be summarized as follows: (1) immediate allorecognition occurs accompanied by microscopic cellular rearrangements; (2) macroscopic tissue bridges form across the graft interface; (3) massive cellular infiltration towards graft sites, with cells often passing into a graft partner through the tissue bridges; (4) cellular hyperplasia and then cell death occurs at the interface; and (5) sloughing of dead tissue leaves areas of vacant skeleton between the two grafted individuals. Allograft rejection reactions in sponges have many of the hallmarks of histoincompatibility reactions in higher animals. Although there could be different alloimmune mechanisms operating in different animals, the salient events of allorecognition and antagonistic rejection appear strikingly similar among diverse multicellular animals.     

Cyst formation of the noxious red tide flagellate Chattonella marina (Raphidophyceae) in culture

Cyst formation of the noxious red tide flagellate Chattonella marina (Raphidophyceae), cultured from a cyst in sediments of Suo-nada, Japan in 1986, was investigated by culture experiments. Nitrogen limitation was effective in inducing cyst formation. After incubation in N-limited medium at 25°C with 10000 lux on a 14 h light, 10 h dark photo-cycle, cultures were subjected to six different light intensities (0 10000 lux) in order to determine effects of light intensity on cyst formation. Cysts were formed under light intensities of 1000 lux or below, but not at 10000 lux. Cysts formed in culture displayed morphological characteristics quite similar to those natural cysts observed in sediments collected from the Inland Sea of Japan. Observations on nucleus, using diaminidinophenylindole (DAPI)-staining and epifluorescence microscopy, indicated that the majority of cysts formed in culture were uninucleate, a few were binucleate. Germination of cysts was observed under adequate conditions for germination (22°C, 3500 lux with a 14 h light, 10 h dark photo-cycle) even after storage at 11°C in the dark for more than 4 mo.     

Longitudinal Dispersion Characteristics of Rivers and Natural Streams in Greece

An optimized parameterization of the non-dimensional longitudinal dispersion coefficients in Greek rivers, $K_{x_a } $ , is described in terms of parameters derived from cross-section river geometry and longitudinal velocity data. Optimization is accomplished, using various combinations of the selected parameters, either for subsets of $K_{x_a } $ values, according to the cross-section aspect ratio range, or for the entire $K_{x_a } $ population. The use of shape factors associated with the river cross-section transverse profiles of depth-mean longitudinal velocities and the normalized intensities of longitudinal velocity variations from the overall mean cross-sectional velocity improves the parameterization. The statistical behavior of $K_{x_a } $ in terms of skewness, flatness and other shape coefficients is also examined. $K_{x_a } $ histograms show that these coefficients have a log-normal distribution. An empirical expression for $K_{x_a } $ is also proposed that takes into account both contributions from the transverse velocity profile and from the vertical profile of longitudinal velocities, at each cross-section, in shaping the value of dispersion coefficients when the aspect ratio of the cross-section is of O(1) and/or when $K_{x_a } $ is of O(1).     

Influences of Magnesium <Emphasis Type="Italic">tri-</Emphasis>Silicate on the Physical,Mechanical, and Degradable Properties of Ultraviolet (UV) Radiation Cured Plain Board Surface

A series of formulations were prepared with different percentages of oligomer, epoxy diacrylate (EA-1020 ), monomer, 1,6 Hexane diol diacrylate,(HDDA) and different percentages of filler (Magnesium tri-silicate, Mg₂Si₃O₈). Irgacure 369 [2-Benzyl-2-dimethyl-amine-1 (4-morpholinophenyl) butanone-1] was used in the formulations as photoinitiator. Ultraviolet (UV) cured thin polymer films were prepared from these formulating solutions on clean glass plates. Pendulum hardness (PH), gel content and macro scratch hardness (MSH) of the UV cured films were studied. One percent Mg₂Si₃O₈ containing formulation showed the premium properties. The substrates (plain board) were coated by these formulating solutions and cured under the same UV lamp at different intensities of radiation. Various properties of the coated surface such as PH, gloss, adhesion, abrasion and MSH were investigated. The base coat containing 1% Mg₂Si₃O₈ and top coat containing 48% HDDA produced the best performance among all the formulations inspected. The degradable properties in different weathering conditions on PH, gloss, adhesion, abrasion and MSH were measured. The surface cured with the optimized formulation (E) again yielded the minimum loss of the properties.     

Surface retention and photochemical reactivity of the diphenylether herbicide oxyfluorfen

The photochemical behavior of oxyfluorfen [2-chloro-1-(3-etoxy-4-nitrophenoxy)-4-(trifluoromethyl) benzene] on two Greek soils was investigated. Soils were sampled from Nea Malgara and Preveza regions, characterized by a different organic matter content. Soils were spiked with the diphenyl-ether herbicide and irradiation experiments were performed either in the laboratory with a solar simulator (xenon lamp) or outside, under natural sunlight irradiation; other soil samples were kept in the dark to control the retention reaction. Kinetic parameters of both retention and photochemical reactions were calculated using zero-, first- and second- (Langmuir-Hinshelwood) order equations, and best fit was checked through statistical analysis. The soil behaviors were qualitatively similar but quantitatively different, with the soil sampled from the Nea Malgara region much more sorbent as compared with Preveza soil. All studied reactions followed second-order kinetics and photochemical reactions were influenced by retaining capability of the soils. The contributions of the photochemical processes to the global dissipation rates were also calculated. Two main metabolites were identified as 2-chloro-1-(3-ethoxy-4-hydroxyphenoxy)-4-(trifluoromethyl)benzene and 2-chloro-1- (3-hydroxy-4-nitrophenoxy)-4-(trifluoromethyl)benzene.     

Physiological and Biochemical Effects of Simulated Acid Rain on Phaseolus Vulgaris Var. HUR-15

Pot experiments were performed to determine the effects of simulated acid rain on Phaseolus vulgaris var. HUR-15. Seeds of Phaseolus vulgaris var. HUR-15 were exposed to simulated acid rain at pH values of 5.1, 4.1, 3.1, 2.1 and 1.1 and a control level of pH 6.8. Plants were harvested at three stages, i.e. pre, peak and post-flowering stages. Seedlings succumbed at pH 1.1. Necrosis developed on the leaves of plants treated with water of pH 2.1, resulting in adverse effect on chlorophyll content. They died after peak-flowering. Root and shoot length and dry weight were reduced with the lowering in pH. Carbohydrate, nitrogen and protein contents decreased with the decrease in pH level, adversely affecting their nutrient value.