A simple fluorescence anisotropy assay for detection of bisphenol A using fluorescently labeled aptamer

Bisphenol A(BPA) is one of the environmental endocrine disruptors(EDCs), and BPA contamination in environment can cause high risks to human health. Rapid determination of BPA on sites is in high demand in environmental analysis. Taking advantage of aptamers as affinity ligands and fluorescence anisotropy(FA) analysis, we developed a simple and rapid FA assay for BPA by employing a single tetramethylrhodamine(TMR) labeled short 35-mer DNA aptamer against BPA. The assay is based on the BPA-binding...     

2015—2021年广州地区近地面O3浓度污染特征及其与气象因素关系

利用2015—2021年广州地区近地面逐时臭氧（O₃）观测资料及同期地面气象站常规观测数据,分析了广州地区近地面O₃浓度污染特征及其与气象因素的关系.结果表明：2015—2021年广州地区O₃浓度呈缓慢上升趋势,增速为1.9 μg?m^-3?a^-1,2015和2019年O₃浓度超标天数 最多;O₃平均浓度季节变化明显：秋季＞冬季＞夏季＞春季;O₃浓度空间分布不均匀,城郊地区高于中心城区;峰值中心位于城郊地区白云区,低值中心位于中心城区荔湾区.O₃浓度高峰期是7—10月,9月浓度最高,3月浓度最低;四季O₃浓度日变化均呈“单峰型”结构,最低值出现在7：00—8：00,14：00—16：00达到峰值.近地面O₃平均浓度和O₃超标率均与气温呈正比,当气温＞15 ℃开始出现臭氧超标现象.相对湿度＜50%时,O₃超标率与相对湿度呈正比;相对湿度为40%~50%时,O₃超标率达峰值为16.3%.当风速＜2 m?s^-1时,O₃超标率与风速呈正比;当风速＞ 2 m?s^-1时,O₃超标率与风速呈反比.高温、低湿、风小是广州地区产生高浓度O₃的主要气象因子.     

Modeling Carbon and Water Budgets in the Lushi Basin with Biome-BGC

1 INTRODUCTIONEvapotranspiration (ET) is the key process controlling theexchange of energy and hydrologic flux for vegetatedsurface. Terrestrial net primary productivity (NPP)represents the carbon available for plant allocation toleaves, stems, roots, defensive compounds, reproductionand is the basic measure of biological productivity. Treegrowth, forage available for grazing, food productivity,and atmospheric CO2 levels are all strongly controlled byNPP (White et al., 2000). The exch…     

Modeling Carbon and Water Budgets in the Lushi Basin with Biome-BGC

Abstract

In this article, annual evapotranspiration (ET) and net primary productivity (NPP) of four types of vegetation were estimated for the Lushi basin, a subbasin of the Yellow River in China. These four vegetation types include: deciduous broadleaf forest, evergreen needle leaf forest, dwarf shrub and grass. Biome-BGC—a biogeochemical process model was used to calculate annual ET and NPP for each vegetation type in the study area from 1954 to 2000. Daily microclimate data of 47 years monitored by Lushi meteorological station was extrapolated to cover the basin using MT-CLIM, a mountain microclimate simulator. The output files of MT-CLIM were used to feed Biome-BGC. We used average ecophysiological values of each type of vegetation supplied by Numerical Terradynamic Simulation Group (NTSG) in the University of Montana as input ecophysiological constants file. The estimates of daily NPP in early July and annual ET on these four biome groups were compared respectively with field measurements and other studies. Daily gross primary production (GPP) of evergreen needle leaf forest measurements were very close to the output of Biome-BGC, but measurements of broadleaf forest and dwarf shrub were much smaller than the simulation result. Simulated annual ET and NPP had a significant correlation with precipitation, indicating precipitation is the major environmental factor affecting ET and NPP in the study area. Precipitation also is the key climatic factor for the interannual ET and NPP variations.     

混凝＋超滤联用技术净化微污染水的试验研究

采用聚合氯化铝混凝与超滤膜联用技术净化微污染水,考察该工艺净化COD、氯离子、浊度和铁离子的效果。结果表明,该工艺运行稳定,出水水质满足生活饮用水卫生标准（ GB 5749－2001）,混凝＋超滤联用技术不仅能有效地净化有机物、浊度,对铁离子和氯离子也有较好的净化效果,且出水中悬浮物、细菌和大肠菌群数未检测出。     

天津市在用汽车遥感检测分析研究

遥测法是对在用车污染情况进行监督管理的高效、便捷的新技术,利用天津市遥测法对路上行驶的在用车的监测数据,分析了各项污染指标间的关系、同一辆车检测数据的重复性、对在用车的控制效果、不同控制方案下遥测法检测限值,并提出了遥测法检测数据的精度要求。     

集胞藻钙化对Cd胁迫的生理响应

为了研究在藻体钙化情况下Cd对集胞藻的胁迫作用,采用实验室模拟方法,通过不同浓度Ca2 、Cd2 配比下培养基中生物量、光合溶解氧、叶绿素等一系列生理指标的变化来反映钙化条件下Cd2 对集胞藻细胞的胁迫程度.结果显示,钙离子的存在能够使Cd2 对藻细胞的毒害作用减小并保护其受到进一步损害;二因素影响下交互试验,发现Ca2 、Cd2 分别与生物量呈现明显的负相关(|FCa2 |＞F0.05|FCd2 |＞F0.05),两者的交互作用与其呈显著正相关(FCd2 ＞F0.05).SEM和EDS观察到钙化层的产生.这说明重金属Cd对藻体胁迫压力的减小是由于钙化层的保护作用.     

Phylogenetic analysis and arsenate reduction effect of the arsenic-reducing bacteria enriched from contaminated soils at an abandoned smelter site

Microbial reduction of As(V)(i.e.,arsenate)plays an important role in arsenic(As)mobilization in aqueous environment.In this study,we investigated As(V)reduction characteristics of the bacteria enriched from the arsenic-contaminated soil at an abandoned smelter site.It was found that As(V)was completely reduced to As(III)(i.e.,arsenite)in 21 h.After 3-d incubation,a yellow solid was precipitated and the concentration of As(III)decreased sharply.After 150 h incubation,ca.65%of soluble arsenic was removed fro...     

壳聚糖接枝共聚物在污泥脱水中的应用研究

以壳聚糖为原料,通过接枝共聚反应分别合成壳聚糖-AM-DMDAAC,壳聚糖-AM,壳聚糖-AA三种不同类型的天然改性高分子絮凝剂.对活性污泥脱水性能的研究结果表明,与其它两种壳聚糖接枝共聚物相比,壳聚糖-AM-DMDAAC阳离子型接枝共聚物是适合污泥脱水的良好絮凝剂.对于100 mL污泥,当污泥pH值为6.00,投药量为20 mg/L,真空度0.05 MPa下,阳离子型壳聚糖接枝共聚物真空抽滤1 min的滤液体积可达到70 mL,抽滤 5 min 后,可将污泥含水率从95.4%降低到86.4%,脱水效果优于其它几种常用的污泥脱水剂.     

Review on environmental policies in China:Evolvement,features,and evaluation

This article reviews the development of current environmental policies in China and the great progress made during 1979–2006. Applying lessons learned from industrialized countries to its own situation, China’s environmental policies have the following main features: (1) to explore command and control measures to their fullest extent, (2) to strive to raise funding for environmental protection, (3) to identify who should take accountability for environmental protection; (4) to encourage strategies of “combination of prevention and control” and “integrated utilization”, (5) to embrace openness in the field of environmental policy and early international cooperation. During the past 30 years, China’s environmental policies have evolved and deepened: status expanded the national basic policies of environmental protection by adding a sustainable development strategy, focus changed from pollution control to a combination of pollution control and ecological protection, method changed from end control to source control, scope changed form point source control to watershed and territory pollution control, and management style changed from primarily using executive power to using legal and economic measures. This article introduces how they are evaluated by the international community and provides the prospects of the policies.