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31.
Frans J. Los PhD Adriana M. Hagenaars Titia E. Cohen-Overbeek Hendrik W. P. Quartero 《黑龙江环境通报》1994,14(7):565-568
The levels of the maternal serum markers alpha-fetoprotein (AFP), human chorionic gonadotrophin (hCG), and unconjugated oestriol (uE3) in 35 pregnant women with early second-trimester oligohydramnios differed from those in a reference population of 1699 singleton pregnancies. Maternal serum AFP levels above the 95th centile of the population distribution were observed in 80 per cent (16/20) of oligohydramnios cases with a normal fetus and in only 20 per cent (3/15) of the cases with a fetus displaying urogenital tract malformations. Elevated levels of hCG (above the 95th centile) and decreased levels of uE3 (below the fifth centile) were encountered in 26 per cent (9/35) and 17 per cent (6/35) of the women, irrespective of the fetal condition. The abnormal profile of the serum markers in early second-trimester oligohydramnios resulted in 57 per cent (20 out of 35) of screen-positive cases for either fetal Down's syndrome or neural tube defects, compared with 8·4 per cent (143 out of 1699) in the reference population. 相似文献
32.
Diane van Opstal Cardi van den Berg Milena G. J. Jahoda Helen Brandenburg Frans J. Los Peter A. In'T Veld 《黑龙江环境通报》1995,15(1):51-55
Trisomy 18 in direct chorionic villus preparations needs further investigation since the chromosome abnormality may be confined to the placenta and may not represent the actual fetal karyotype. We performed, retrospectively, fluorescent in situ hybridization (FISH) with the chromosome 18 centromere probe (L1.84) on interphase nuclei of destained slides of all cases of full trisomy 18 (n=22) and mosaic trisomy 18 (n=8) detected among 7600 first-trimester chorionic villus samples during an 8-year period (1985–1992). More nuclei displaying three signals were encountered in cases of full and mosaic trisomy 18 confirmed in fetal tissue than in non-confirmed cases. FISH can be useful for the verification of trisomy 18 in direct chorionic villus preparations. 相似文献
33.
Juriy W. Wladimiroff MD Ph.D. Wydia R. Bhaggoe Miranda Kristelijn Titia E. Cohen-Overbeek Nicolette S. Den Hollander Helen Brandenburg Frans J. Los 《黑龙江环境通报》1995,15(5):431-438
Structural pathology and outcome were studied in 170 chromosomally abnormal fetuses. Numerical chromosomal abnormalities were established in 158 (93 per cent) cases, of which 110 (71 per cent) represented trisomies, 30 (18 per cent) Turner syndrome, and 18 (11 per cent) triploidy. Structural chromosomal abnormalities were diagnosed in 12 (7 per cent) cases. Gestational age at referral was significantly shorter for pregnancies with Turner syndrome than for the other chromosomal abnormalities. Referral before 20 weeks of gestation was mainly based on fetal structural pathology alone (92 per cent); after 20 weeks, patients were referred because of structural pathology combined with small for gestational age, oligohydramnios, or polyhydramnios. Referral as a result of suspected multiple organ pathology occurred in 73.5 per cent of pregnancies. An abnormal amniotic fluid volume was present in 59/170 (34.5 per cent) chromosomally affected pregnancies, i.e., oligohydramnios in 31 and polyhydramnios in 28 cases. Birth weight was below the tenth percentile in over half of the chromosomally abnormal fetuses, except for Turnersyndrome. Fetal outcome was poor, with a survival rate at 1 month of 30 per cent for trisomies which was mainly determined by trisomy 21 (14/18=77.5 per cent). 相似文献
34.
Christoph Brezinka Adriana M. Hagenaars Professor Juriy W. Wladimiroff MD PhD Frans J. Los 《黑龙江环境通报》1995,15(8):699-703
Doppler flow velocity waveform recording in the fetal ductus venosus and umbilical artery as well as maternal blood sampling for serum alpha-fetoprotein (MSAFP) was performed before and after transabdominal chorion villus sampling (TACVS) in 36 women of advanced maternal age (≥ 36 years). Gestational age ranged between 11 and 13 weeks. No chromosomal anomaly was detected. No statistically significant difference was observed in ductus venosus velocity parameters or in the umbilical artery pulsatility index (PI) before and after CVS in 35 women with a normal pregnancy outcome. One case resulted in fetal loss. Post-CVS median MSAFP levels at 12 weeks (25 kIU/1) and 13 weeks (35 kIU/1) were significantly higher than pre-CVS levels. In three cases, post-CVS MSAFP levels were higher than 600 kIU/1, correlating with feto-maternal transfusions of approximately 1.0–1.4 ml, i.e., of around 40 per cent of feto-placental blood volume. One of these cases displayed absence of fetal peripheral blood flow velocities and fetal bradycardia following TACVS, resulting in fetal loss 1 week later. The remaining two cases had a normal pregnancy outcome, but showed a more than 50 per cent reduction in ductus venosus velocity after TACVS, whereas umbilical artery PI remained unchanged. However, similar velocity changes were associated with only small feto-maternal transfusions. Umbilical artery PI values remained unchanged. 相似文献
35.
M. G. J. Jahoda H. Brandenburg A. Reuss T. E. Cohen-Overbeek J. W. Wladimiroff F. J. Los E. S. Sachs 《黑龙江环境通报》1991,11(8):559-561
Data from 3611 consecutive CVS (TC, N= 1780; TA, N= 1831) were analysed with emphasis put on influence of maternal and gestational age at CVS on the fetal loss rate < 28 weeks. For TC-CVS the gestational age varied from 9.3–11.6 weeks, for TA-CVS from 9.3–20 weeks. Sampling efficacy at first attempt was 86.5 per cent and 95 per cent respectively. In 4.6 per cent an abnormal result was established. In older mothers (N=2362) the fetal loss rate was significantly higher (p = <0.05) when sampled before 12 weeks (TC-CVS 6.2 per cent, TA-CVS 5.8 per cent). When the CVS (TA) was performed after 12 weeks the fetal loss rate decreased to 2.4 per cent. In 1079 younger women the fetal loss rate remained low (TC 2.8 per cent; TA < 12 weeks 1.8 per cent; TA > 12 weeks 1.7 per cent) and was not influenced by gestational age at the time of sampling. We concluded both methods safe and reliable when the choice of application considers maternal age. 相似文献
36.
M. G. J. Jahoda M.D. L. Pijpers A. Reuss F. J. Los J. W. Wladimiroff E. S. Sachs 《黑龙江环境通报》1989,9(9):621-628
Data from 1550 consecutive pregnancies after first-trimester prenatal diagnosis by transcervical chorionic villus sampling (TC-CVS) are presented. The sampling efficacy was 97.8 per cent; the mean amount of collected villus tissue was 23 mg (range 5–100 mg). There were 97 affected fetuses, mainly (73.2 per cent) with a chromosomal abnormality or a male karyotype in carriers of X-linked disease. Pregnancy termination in these and four other women for social reasons resulted in 1449 continuing pregnancies. In these pregnancies, the fetal loss rate up to 28 weeks of gestation was 5.1 per cent with the highest loss rate (3.9 per cent) before 16 weeks. When relating this fetal loss rate to maternal age, this was 6.1 per cent in the advanced maternal age group (⩾36 years) against 3.1 per cent in the younger age group. In 1376 pregnancies continuing beyond 28 weeks, the perinatal mortality rate was 1.1 per cent; the percentage of non-genetic congenital anomalies was 0.9 per cent. The reproductive pattern of women at high genetic risk after CVS followed by pregnancy termination was evaluated. Within 12 months after the first CVS followed by pregnancy termination, 70 percent of women again requested CVS in a subsequent pregnancy. 相似文献
37.
Javier Miranda Ihali Crespo Maria Angeles Morales 《Environmental science and pollution research international》2000,7(1):14-18
A receptor model is presented based on absolute principal component analysis (APCA) of elemental concentrations in atmospheric aerosols from Mexico City during the Summer of 1995. Elemental contents on samples collected with a Stacking Filter Unit of the Davis design was carried out using Particle Induced X-ray Emission (PIXE). The sampling device allowed the separation of particles with mean aerodynamic diameters ranging from 2.5 μm to 15 μm (coarse fraction) and smaller than 2.5 μm (fine fraction). Sampling was divided into morning, afternoon and night periods, with higher concentrations being found during the morning. Seasonal variation is observed when comparisons with other studies are carried out. The application of APCA allowed identification of four sources for each fraction, with a soil-derived dust predominance in the coarse one. The influence of meteorological parameters is studied using cluster analysis, showing that during the morning there is a transport of pollutants from the west towards the sampling site, while the night transport corresponds to soil-derived dust from the north. 相似文献
38.
The cyanobacterial toxin Cylindrospermopsin (CYN) is receiving great interest due to its increasing presence in waterbodies, which has lead to recognize it as a potential threat to drinking water safety. CYN is a potent inhibitor of protein and glutathione synthesis. The present work studies for the first time the effects of CYN in endothelial cells. The basal cytotoxicity endpoints studied at 24 and 48 h were total protein content (PC), neutral red (NR) uptake and the tretazolium salt, MTS, reduction. Moreover, the effect of subcytotoxic concentrations of CYN on the generation of intracellular reactive oxygen species (ROS), the activity of γ-glutamylcysteine synthetase (GCS) and glutathione (GSH) content have been investigated. In addition, morphological alterations of HUVEC cells subsequent to CYN exposure were recorded.The cytotoxicity endpoints revealed a decrease in the cellular viability in a time and concentration-dependent way. The most sensitive cytotoxicity endpoint was NR uptake assay, with reductions in cell viability of 95% at 48 h of exposure to 40 μg mL−1 CYN. Intracellular ROS production was increased only at the lowest concentration assayed, while GCS activity and GSH content underwent concentration-dependent enhancements. The most remarkable morphological alterations observed were: nucleolar segregation with altered nuclei, degenerated Golgi apparatus, increases in the presence of granules and apoptosis. 相似文献
39.
Viñas L Franco A Blanco X Bargiela J Soriano JA Perez-Fernandez B Gonzalez JJ 《Environmental science and pollution research international》2012,19(2):529-539
The aim of this study was to establish the temporal trends and spatial distribution of Polycyclic Aromatic Hydrocarbons (PAHs)
in Ria de Vigo by means of studying the PAH burden in wild mussels. The samples were collected in eight sites along the margins
of the Ria de Vigo during November from 1998 to 2008 and Polycyclic Aromatic Hydrocarbon contents were determined using high
performance liquid chromatography–fluorescence derivatization (HPLC–FLD). Quality of chemical analysis was guaranteed by participation
in QUASIMEME intercalibration exercises carried out during the period of analysis. The concentrations for the sum of 13 PAHs
were in the range 24–480 μg/kg dw, typical of an urban and industrialized area (50–500 μg/kg dw) except for a punctual input
in La Guía in 1998 that led to a concentration above 1,000 μg/kg dw. In general, the sites in the outermost section of the
estuary (ría) showed the lowest values, with an increasing gradient in the medium and inner parts of the ría. Temporal patterns
show, in general, a downward trend. The relative proportion of 3 and 4 ring PAHs also varies with the site, the former being
predominant in the outermost area and the latter in the medium and inner parts of the ría. This is consistently related with
the sources and processes taking place in different areas of the estuary. 相似文献
40.
Intercomparison exercise for gases emitted by a cement industry in Spain: a functional data approach
Ordòñez C Martìnez J Saavedra A Mourelle A 《Journal of the Air & Waste Management Association (1995)》2011,61(2):135-141
This paper describes the results of an intercomparison exercise referring to the measurement of atmospheric pollutants emitted by cement plants. The research was conducted in 2008 in Catalonia, Spain. Thirteen Spanish companies accredited to make pollutant measurements and with suitably approved equipment and trained staff participated in the research. The aim of the research was to evaluate the technical competence of the accredited companies in conducting tests in situ. The results obtained from the application of the methodology described in international standards--on the basis of conventional statistical analyses--were compared with those obtained using functional data analysis. On comparing the two methods, it was concluded that the functional method, although more complex, has advantages over the conventional statistical method. This is because the functional approach compares curves rather than mean values. Furthermore, it does not presuppose a normal distribution of the data. 相似文献