Leaching of lead metallurgical slag in citric solutions--implications for disposal and weathering in soil environments

Metallurgical slags from primary lead smelting were submitted to a 30-day batch leaching procedure in 20 and 8 mM citric solutions in order to determine the kinetics of release of Pb, Cu, Zn and As. The experiment was coupled with the PHREEQC-2 speciation-solubility modelling and mineralogical study of newly formed products (SEM/EDS, XRD, TEM/EDS and Raman spectrometry). A strong scavenging of metals and metalloids from the 8 mM citric leachate was observed due to the formation of newly formed products. The secondary precipitate consisted of well-developed calcite (CaCO3) crystals and amorphous organo-mineral matrix composed of hydrous ferric oxides and amorphous SiO2. Metals (Pb, Zn, Cu) and arsenic released into the solution were subsequently bound onto the newly formed product (adsorption on oxides) or trapped within the calcite structure (Zn, Mn). Similar scavenging mechanism can be taken into account in real soil systems with lower concentration of citric acid. Then, the covering of slag dumps with a thick soil layer and subsequent re-vegetation might be a possible scenario for slag management on some metallurgical sites.     

Effects of multiple but low pesticide loads on aquatic fungal communities colonizing leaf litter

In the first tier risk assessment (RA) of pesticides, risk for aquatic communities is estimated by using results from standard laboratory tests with algae, daphnids and fish for single pesticides such as herbicides, fungicides, and insecticides. However, fungi as key organisms for nutrient cycling in ecosystems as well as multiple pesticide applications are not considered in the RA. In this study, the effects of multiple low pesticide pulses using regulatory acceptable concentrations (RACs) on the dynamics of non-target aquatic fungi were investigated in a study using pond mesocosm. For that, fungi colonizing black alder (Alnus glutinosa) leaves were exposed to multiple, low pulses of 11 different pesticides over a period of 60 days using a real farmer''s pesticide application protocol for apple cropping. Four pond mesocosms served as treatments and 4 as controls. The composition of fungal communities colonizing the litter material was analyzed using a molecular fingerprinting approach based on the terminal Restriction Fragment Length Polymorphism (t-RFLP) of the fungal Internal Transcribed Spacer (ITS) region of the ribonucleic acid (RNA) gene(s). Our data indicated a clear fluctuation of fungal communities based on the degree of leaf litter degradation. However significant effects of the applied spraying sequence were not observed. Consequently also degradation rates of the litter material were not affected by the treatments. Our results indicate that the nutrient rich environment of the leaf litter material gave fungal communities the possibility to express genes that induce tolerance against the applied pesticides. Thus our data may not be transferred to other fresh water habitats with lower nutrient availability.     

Commentary: Status of road safety in Asia

Objectives: The objective of this article is to assess the status of road safety in Asia and present accident and injury prevention strategies based on global road safety improvement experiences and discuss the way forward by indicating opportunities and countermeasures that could be implemented to achieve a new level of safety in Asia.

Methods: This study provides a review and analyses of data in the literature, including from the World Health Organization (WHO) and World Bank, and a review of lessons learned from best practices in high-income countries. In addition, an estimation of costs due to road transport injuries in Asia and review of future trends in road transport is provided.

Results: Data on the global and Asian road safety problem and status of prevention strategies in Asia as well as recommendations for future actions are discussed. The total number of deaths due to road accidents in the 24 Asian countries, encompassing 56% of the total world population, is 750,000 per year (statistics 2010). The total number of injuries is more than 50 million, of which 12% are hospital admissions. The loss to the economy in the 24 Asian countries is estimated to around US$800 billion or 3.6% of the gross domestic product (GDP).

Conclusions: This article clearly shows that road safety is causing large problems and high costs in Asia, with an enormous impact on the well-being of people, economy, and productivity. In many Asian low- and middle-income countries, the yearly number of fatalities and injuries is increasing. Vulnerable road users (pedestrians, cyclists, and motorcyclists combined) are particularly at risk. Road safety in Asia should be given rightful attention, including taking powerful, effective actions. This review stresses the need for reliable accident data, because there is considerable underreporting in the official statistics. Reliable accident data are imperative to determine evidence-based intervention strategies and monitor the success of these interventions and analyses. On the other hand, lack of good high-quality accident data should not be an excuse to postpone interventions. There are many opportunities for evidence-based transport safety improvements, including measures concerning the 5 key risk factors: speed, drunk driving, not wearing motorcycle helmets, not wearing seat belts, and not using child restraints in cars, as specified in the Decade of Action for Road Safety 2011–2020. In this commentary, a number of additional measures are proposed that are not covered in the Decade of Action Plan. These new measures include separate roads or lanes for pedestrians and cyclists; helmet wearing for e-bike riders; special attention to elderly persons in public transportation; introduction of emerging collision avoidance technologies, in particular automatic emergency braking (AEB) and alcohol locks; improved truck safety focusing on the other road user (including blind spot detection technology; underride protection at the front, rear, and side; and energy-absorbing fronts); and improvements in motorcycle safety concerning protective clothing, requirements for advanced braking systems, improved visibility of motorcycles by using daytime running lights, and better guardrails.     

Application of high silica zeolite ZSM-5 in a hybrid treatment process based on sequential adsorption and ozonation for VOCs elimination

In this study, a hydrophobic synthetic zeolite, namely ZSM-5 is chosen as an adsorbent/catalyst for toluene removal. Experimental results showed that toluene adsorption onto ZSM-5 was favourable, following a Langmuir adsorption isotherm model. ZSM-5 zeolite was regenerated using gaseous ozone at low temperature. Adsorbed toluene was oxidised, releasing mainly CO₂ and H₂O. Traces of oxidation by-products such as acetic acid and acetaldehyde were formed and remained adsorbed after the oxidativate regeneration with ozone. After four successive cycles of adsorption/ozonation, the adsorption efficiency was not affected (92%–99%). These results showed that volatile organic compound (VOC) removal by adsorption onto ZSM-5 zeolite followed by ozone regeneration could be used as a promising hybrid process for the control of VOC emissions in terms of efficiency.     

The Effect of Heat on the Physicochemical Properties of Bacteriophage MS2

The differences in physicochemical characteristics between infectious and non-infectious viral particles are poorly known. Even for heat, which is known as one of the most efficient treatments to inactivate enteric viruses, the global inactivation mechanisms have not been described yet. Such knowledge would help distinguish between both types of particles and therefore clarify the interpretation of the presence of viral genomes in food after heat treatment. In this study, we examined in particular the differences in electrostatic charge and hydrophobicity between the two particle types. MS2 phage, a common surrogate for enteric viruses, was used as a model virus. The heat-induced inactivation process of the infectious phages caused hydrophobic domains to be transiently exposed and their charge to become less negative. The particles also became progressively permeable to small molecules such as SYPRO Orange dye. The presence of non-infectious phage particles in which the genome was not accessible to RNases has been clearly demonstrated. These observations were done for MS2 phages exposed to a temperature of 60 °C. When exposed to a temperature higher than their critical temperature (72 °C), the particles were disrupted and the genome became available for RNases. At lower temperatures, 60 °C in this study, the transient expression of hydrophobic domains of remaining infectious phages appeared as an interesting parameter for improving their specific detection.     

Environment-specific elasticity and sensitivity analysis of the stochastic growth rate

For matrix population models, analyses of how sensitive the population growth rate is to changes in vital rates (i.e. perturbations) are important for studies of life history evolution as well as for management and conservation of threatened species. There are two types of sensitivity analyses corresponding to absolute (sensitivity) or relative (elasticity) changes in the vital rates and both types can be applied to both deterministic and stochastic matrix population models. To date, most empirical studies of elasticity and sensitivity of the stochastic growth rate have examined the response to perturbations in the vital rates in a complete set of possible environments. However, it is often of interest to examine the response to perturbations occurring in only a subset of the possible environments. This has been done for periodic time-varying models elsewhere, but here we describe a recently published method for calculating the environment-specific sensitivity and elasticity of the stochastic growth rate and apply this method to data. These environment-specific perturbation analyses provide a logical way of dividing the sensitivity and elasticity among the environments. They give important insight into the selection regime in different environments and can provide valuable information for making management decisions and management evaluations in stochastic environments.     

Persistent near-bottom aggregations of mesopelagic animals along the North Carolina and Virginia continental slopes

Submersible observations during four missions over the North Carolina and Virginia continental slopes (184–900 m) documented the occurrence of large aggregations of mesopelagic fishes and macronektonic invertebrates near or on the bottom. Aggregated mesopelagics formed a layer up to tens of meters deep positioned from a few centimeters to 20 m, usually <10 m, above the substrate. Aggregations were numerically dominated by microvores, notably the myctophid fish Ceratoscopelus maderensis and the penaeid shrimp Sergestes arcticus. Consistently present but in relatively lower numbers, were mesopelagic predators, including the paralepidids Notolepis rissoi and Lestidium atlanticum, the eel Nemichthys scolopaceus, the stomiid fishes Chauliodus sloani and Stomias boa ferox, and squids Illex spp. Near-bottom aggregations do not appear to be an artifact due to attraction to the submersible. Based on submersible observations in three areas in 4 years spanning a decade, near-bottom aggregations of midwater organisms appear to be a geographically widespread and persistent phenomenon along the continental slope of the southeastern US Aggregations may exploit areas of enhanced food resources at the bottom.