Triterpene saponin hemi-biosynthesis of a leaf beetle’s (<Emphasis Type="Italic">Platyphora kollari</Emphasis>) defensive secretion

The adults of the leaf beetle Platyphora kollari (Chrysomelidae) are able to metabolise the oleanane triterpene β-amyrin (1) into the glycoside 3-O-β-d-glucopyranosyl-(1→4)-β-d-glucuronopyranosyl-hederagenin (2) that is stored in their defensive glands. The aim of this study was to test the hypothesis that oleanolic acid (3) is an intermediate in the conversion of 1 into 2 and to check whether the sequestration of pentacyclic triterpenes is selective in favour of β-amyrin (1). To this end, adults of P. kollari were fed with Ipomoea batatas leaf disks painted with a solution of [2,2,3-²H₃]oleanolic acid or [2,2,3-²H₃]α-amyrin and the secretion of their defensive glands analysed by HPLC–ESIMS. The data presented in this work indicated that the first step of the transformation of β-amyrin (1) into the sequestered glycoside 2 is its oxidation into oleanolic acid (3) and that this conversion is selective but not specific in favour of β-amyrin (1).     

Conditions controlling the onset of breeding migration of the Japanese mountain stream frog, <Emphasis Type="Italic">Rana sakuraii</Emphasis>

I studied the conditions controlling the timing of breeding migration of the Japanese mountain stream frog Rana sakuraii, an explosive breeder, over 9 years (1992, 1993 and 1999–2005). I analysed two factors: the daily cumulative temperature (DCT) during hibernation and the triggering temperature on the day of migration onset. Frogs hibernated in shallow running water in streams in December. A total of 53,155 breeding migrating adults were captured by traps. Every year, breeding migration was induced by a rise in daily maximum water temperature to about 5°C. However, its date was limited to 1 February at the earliest, and the onset needed a DCT (from 20 January, using 3°C as the threshold for daily effective temperature) of at least about 15°C. Earlier (e.g. in mid- or late January), even if the maximum temperature rose to 5–8°C, migration did not begin. Moreover, even in early February, if the maximum temperature rose to 5°C, if it had been too cold in January and the DCT was low, migration would not begin until mid- or late February. Thus, the earliest date of readiness for migration varied from 1 February to mid-February, depending on the winter DCT. Logistic regression analyses showed that both factors, the DCT and the daily temperature, were significant. I propose that in temperate-zone amphibian explosive breeders, both the passing of an essential number of days and an essential DCT during hibernation are prerequisites for the onset of breeding migration before the daily temperature rises to the threshold.     

Further evidence of nuclear reactions in the Pd/D lattice: emission of charged particles

Almost two decades ago, Fleischmann and Pons reported excess enthalpy generation in the negatively polarized Pd/D-D₂O system, which they attributed to nuclear reactions. In the months and years that followed, other manifestations of nuclear activities in this system were observed, viz. tritium and helium production and transmutation of elements. In this report, we present additional evidence, namely, the emission of highly energetic charged particles emitted from the Pd/D electrode when this system is placed in either an external electrostatic or magnetostatic field. The density of tracks registered by a CR-39 detector was found to be of a magnitude that provides undisputable evidence of their nuclear origin. The experiments were reproducible. A model based upon electron capture is proposed to explain the reaction products observed in the Pd/D-D₂O system.

Male satin bowerbirds (<Emphasis Type="Italic">Ptilonorhynchus violaceus</Emphasis>) compensate for sexual signal loss by enhancing multiple display features

Numerous studies have focussed on the relationship between female choice and the multiple exaggerated sexual traits of males. However, little is known about the ability of males to actively enhance specific components of their display in response to the loss of one component. We investigated the capacity of male satin bowerbirds (Ptilonorhynchus violaceus) to respond to the loss of one of their sexual signals by performing an experiment in which we removed decorations at their bowers. We found that males compensated for decoration loss by increasing bower construction behaviour and decreasing their latency to bower painting. These results are novel because they suggest that males can assess the quality of their own display and make decisions about how to augment their displays. We discuss these results in the context of previous studies of mate choice in satin bowerbirds, as both of the supplementary behaviours we observed are known correlates of male mating success.     

Rollover injury: effects of near- and far-seating position, belt use, and number of quarter rolls

PURPOSE: Vehicle and occupant responses in rollovers are complex since many factors influence both. This study analyzes the following factors: 1) belt use, 2) seated position with respect to the lead side in the rollover, 3) another front occupant in the crash, and 4) number of quarter rolls. The aim was to improve our understanding of rollover injury mechanisms. METHOD: Rollover accidents were analyzed using 1992-2004 NASS-CDS data. The sample included adult drivers and right-front passengers. All occupants were evaluated and then a subset of non-ejected occupants was analyzed. Using roll direction and seating position, the sample was divided into near- and far-seated occupants. Injury and fatality risks were determined by seatbelt use, occupancy, rollover direction, and number of quarter rolls. Risk was defined as the number of injured (e.g., MAIS 3+) divided by the number of exposed occupants (MAIS 0-6). Significance in differences was determined. A matched-pair analysis was used to determine the risk of serious injury for near- and far-seated occupants who were either belted or unbelted in the same crash. RESULTS: For all occupants, serious injury risks were highest for far-seated, unbelted occupants at 18.1% +/- 4.8%, followed by near-seated unbelted occupants at 12.0% +/- 3.5%. However, the difference was not statistically significant. Belted near- and far-seated occupants had a similar injury risk of 4.3% +/- 1.2% and 4.0% +/- 1.2%, respectively. For non-ejected occupants, serious injury risk was 9.5% +/- 3.2% for far-seated unbelted occupants and 4.9% +/- 2.1% for near-seated unbelted occupants, not a statistically significant difference. Serious injury risk was similar for belted near- and far-seated non-ejected occupants, at 3.6% +/- 1.1%. Seatbelts were 64.2%-77.9% effective in preventing serious injury for all occupants and 62.1%-26.5% for far- and near-seated, non-ejected occupants, respectively. Based on the matched pairs, seatbelts were less effective for near-seated (5.0%) compared to far-seated (2.8%) occupant MAIS 3+F risks. This was similar for non-ejected occupants. An unbelted near-seated occupant increased the risk for a belted far-seated occupant by 2.2 times, whereas an unbelted far-seated occupant increased the risk for a belted near-seated occupant by 10.2 times. For all occupants, the risk of serious injury increased with the number of quarter rolls, irrespective of seated position. For near-seated occupants, seatbelt effectiveness was higher in < or =1 roll than 1+ roll, at 72.3% compared to 28.3%. For far-seated occupants, seatbelt effectiveness was similar in < or =1 and 1+ roll samples at 78.3% and 76.8%, respectively. Near-seated occupants had the lowest serious injury risk when they were the sole occupant in the vehicle. This was also true for non-ejected occupants. However, far-seated occupants had a lower injury risk when another occupant was involved in the crash. CONCLUSIONS: The effect of carrying another occupant appears to reduce the risk of serious injury to far-seated occupants. However, near-seated occupants are better off being the sole occupant in the vehicle. Seatbelt effectiveness was lowest at 28.3% for non-ejected, near-seated occupants in 1+ rolls. This finding deserves further evaluation in an effort to improve seatbelt effectiveness in rollovers. For belted drivers alone in a rollover, fatality risks are 2.24 times higher for the far- versus near-seated position. Analysis of rollovers by quarter turns indicates that occupants are both far-side and near-side in rollovers. The extent to which this confounds the relationship between roll direction, seating position, and injury risk is unknown.     

Prenatal cannibalism in an insect

Host selection and infection strategies of parasitoids often correlate with high parental investment and low numbers of progeny. In this study, we investigate how additional internal mechanisms might shape brood size and fitness of the offspring. Emblemasoma auditrix is a parasitoid fly in which about 38 larvae hatch simultaneously in utero. After host location, a single larva is deposited into the host, where it rapidly develops and pupates after about 5 days. The search for hosts can take several weeks, and during that time, the larvae arrest their development and remain in the first larval instar. Nevertheless, the larvae increase in weight within the uterus, and this growth correlates to a decrease in the number of larvae, although no larvae are deposited. Thus, our data indicate a first case of prenatal cannibalism in an invertebrate with larvae feeding on each other within the uterus of the adult.     

Experience,but not distance,influences the recruitment precision in the stingless bee <Emphasis Type="Italic">Scaptotrigona mexicana</Emphasis>

Recruitment precision, i.e. the proportion of recruits that reach an advertised food source, is a crucial adaptation of social bees to their environment. Studies with honeybees showed that recruitment precision is not a fixed feature, but it may be enhanced by factors like experience and distance. However, little is known regarding the recruitment precision of stingless bees. Hence, in this study, we examined the effects of experience and spatial distance on the precision of the food communication system of the stingless bee Scaptotrigona mexicana. We conducted the experiments by training bees to a three-dimensional artificial patch at several distances from the colony. We recorded the choices of individual recruited foragers, either being newcomers (foragers without experience with the advertised food source) or experienced (foragers that had previously visited the feeder). We found that the average precision of newcomers (95.6 ± 2.61%) was significantly higher than that of experienced bees (80.2 ± 1.12%). While this might seem counter-intuitive on first sight, this “loss” of precision can be explained by the tendency of experienced recruits to explore nearby areas to find new rewarding food sources after they had initially learned the exact location of the food source. Increasing the distance from the colony had no significant effect on the precision of the foraging bees. Thus, our data show that experience, but not the distance of the food source, affected the patch precision of S. mexicana foragers.     

Land use change and forestry climate project regional baselines: a review

Climate change programs have largely used the project-specific approach for estimating baseline emissions of climate mitigation projects. This approach is subjective, lacks transparency, can generate inconsistent baselines for similar projects, and is likely to have high transaction costs. The use of regional baselines, which partially addresses these issues, has been reported in the literature on forestry and agriculture projects, and in greenhouse gas (GHG) mitigation program guidance for them (e.g., WRI/WBCSD GHG Project Protocol, USDOE’s 1605(b) registry, UNFCCC’s Clean Development Mechanism). This paper provides an assessment of project-specific and regional baselines approaches for key baseline tasks, using project and program examples. The regional experience to date is then synthesized into generic steps that are referred to as Stratified Regional Baselines (SRB). Regional approaches generally, and SRB in particular explicitly acknowledge the heterogeneity of carbon density, land use change, and other key baseline driver variables across a landscape. SRB focuses on providing guidance on how to stratify lands into parcels with relatively homogeneous characteristics to estimate conservative baselines within a GHG assessment boundary, by applying systematic methods to determine the boundary and time period for input data.

Integrated strategies to reduce vulnerability and advance adaptation,mitigation, and sustainable development

Determinants of adaptive and mitigative capacities (e.g., availability of technological options, and access to economic resources, social capital and human capital) largely overlap. Several factors underlying or related to these determinants are themselves indicators of sustainable development (e.g., per capita income; and various public health, education and research indices). Moreover, climate change could exacerbate existing climate-sensitive hurdles to sustainable development (e.g., hunger, malaria, water shortage, coastal flooding and threats to biodiversity) faced specifically by many developing countries. Based on these commonalities, the paper identifies integrated approaches to formulating strategies and measures to concurrently advance adaptation, mitigation and sustainable development. These approaches range from broadly moving sustainable development forward (by developing and/or nurturing institutions, policies and infrastructure to stimulate economic development, technological change, human and social capital, and reducing specific barriers to sustainable development) to reducing vulnerabilities to urgent climate-sensitive risks that hinder sustainable development and would worsen with climate change. The resulting sustainable economic development would also help reduce birth rates, which could mitigate climate change and reduce the population exposed to climate change and climate-sensitive risks, thereby reducing impacts, and the demand for adaptation. The paper also offers a portfolio of pro-active strategies and measures consistent with the above approaches, including example measures that would simultaneously reduce pressures on biodiversity, hunger, and carbon sinks. Finally it addresses some common misconceptions that could hamper fuller integration of adaptation and mitigation, including the notions that adaptation may be unsuitable for natural systems, and mitigation should necessarily have primacy over adaptation.

The double trade-off between adaptation and mitigation for sea level rise: an application of FUND

This paper studies the effects of adaptation and mitigation on the impacts of sea level rise. Without adaptation, the impact of sea level rise would be substantial, almost wiping out entire countries by 2100, although the globally aggregate effect is much smaller. Adaptation would reduce potential impacts by a factor 10–100. Adaptation would come at a minor cost compared to the damage avoided. As adaptation depends on socio-economic status, the rank order of most vulnerable countries is different than the rank order of most exposed countries. Because the momentum of sea level rise is so large, mitigation can reduce impacts only to a limited extent. Stabilising carbon dioxide concentrations at 550 ppm would cut impacts in 2100 by about 10%. However, the costs of emission reduction lower the avoided impacts by up to 25% (average 10%). This is partly due to the reduced availability of resources for adaptation, and partly due to the increased sensitivity to wetland loss by adaptation.