The Effect of Military Training Activity on Eastern Lupine and the Karner Blue Butterfly at Fort McCoy,Wisconsin, USA

The US Department of Defense (DOD) manages over 10.1 million ha of land, much of which is used for training military personnel. However, vast sections receive little or no use, and military lands have become refuges for many species. At Fort McCoy, Wisconsin, USA, populations of the endangered Karner blue butterfly (Lycaeides melissa samuelis) are found in oak and pine barren communities where wild lupine (Lupinus perennis), a perennial forb required by Karner blue butterfly larvae, still occurs. Oak and pine barren communities are disturbance-dependent, and the barrens ecosystems in the Midwest have declined in extent by 98% because of fire suppression, succession, and habitat fragmentation. We studied the effects of disturbance by military manuever training on the density of lupine and Karner blue butterfly at Fort McCoy. We also wanted to determine whether military training activity could enhance Karner blue butterfly habitat. At locations where tracked vehicles had driven through lupine patches, the abundance of lupine and nectar-producing plants was greater in the median strip between vehicle ruts than in vehicle ruts or 5 m outside the vehicle ruts. The proportion of lupine stems with Karner blue butterfly larvae feeding sign (the ratio of stems fed upon to stems examined) was greater in areas where military vehicles had traveled than where they had not. The proportion of lupine stems with feeding sign and lupine stem density was also positively related to the occurrence of prior bivouacs and fires caused by military munitions. Shrub and forest canopy abundance were lower in areas traveled by tracked vehicles. At the scale of the lupine patch, lupine abundance and the proportion of lupine stems with feeding sign were positively correlated with military training activities, suggesting that maintenance of lupine habitat can be achieved in concert with military training.     

Main differences on European regulations in the frame of the Seveso Directive

Since the first Seveso Directive was issued 25 years ago, each Member State of the European Union developed own regulations to enforce its application. The lack of a common approach generated differences between Member States, or even between regions within a Member State, which in practical terms caused that establishments falling under the field of application of the Seveso Directive face a different level of requirements. This situation causes significantly different impact on social, technical and financial aspects.Factors like industrial background, accidental history, actual land use, social attitude towards risk, economical and political structures play an important role in how the decisions about hazardous activities are perceived and handled.Answering the question: “Is there a right methodology for the assessment of the risk of major accidents?” may be sterile or impossible. Many tools are appropriate and the main concern is to use them properly. Technically, the causes and consequences of, e.g., a fire in a port in Spain on the Mediterranean Sea or in the port of Basel (Switzerland) in the Rhine River could be assessed using the same methods and criteria, but this is not usually the case.This paper shows, with practical examples, the differences existing while applying different regulations in the field of prevention of major accidents and the potential effects that these differences may generate regarding the definition and management of emergency plans outside establishments.If the existing unequal situation is not faced, the comparative disagreements between Member States will prevail. Therefore, the application of the Seveso Directive will generate a different impact on the society, operators and regulators, and may become a factor which may cause a risk transfer between Member States. Efforts should be addressed to harmonize criteria in this subject.     

Leaf morphoanatomy of species tolerant to excess iron and evaluation of their phytoextraction potential

Setaria parviflora (Poir.) Kerguélen and Paspalum urvillei Steudel are grasses that grow naturally in a soil with high iron contents. This study aimed to characterize morphoanatomically and histochemically the iron phytotoxicity on leaves and evaluate the phytoextraction potential of these grasses. Saplings were cultivated in hydroponic solution with and without excess Fe-EDTA. Regarding measurements taken on leaves, reduction was observed among treatments of Fe-EDTA on height values of abaxial epidermis and bundle sheath in both species. As for iron histolocalization, stronger reaction was observed in leaves of S. parviflora, in comparison with P. urvillei. Anatomical damage, such as protoplast retraction, irregular xylem, changes in cell volume, and cell collapse, and visual symptoms, like leaf bronzing, chlorosis, and necrosis, were similar in both species when exposed to excess iron; however, P. urvillei showed more severe damage. This species accumulated more iron in shoots than S. parviflora and therefore is more favorable for use in phytoextraction. The root system of both species accumulated higher iron concentrations in relation to shoots.     

Photocatalytic degradation of microcystin-LR in aqueous solutions

In this work, the photocatalytic degradation of aqueous microcystin-LR was studied using TiO₂ and ZnO as photocatalysts. The process was optimised and characterised at the bench scale (200 mL); both semiconductors exhibited a high degradation capacity at reaction times of 1 min (degradation greater than 95%). The transient species that were observed indicate that the degradation occurs via the multiple hydroxylation and elimination of the labile peptide residues of the molecule. When photocatalysis was applied in a continuous treatment system (20–50 L), the photocatalytic process exhibited a high degradation efficiency, which resulted in residual microcystin-LR concentrations that were less than 1 μg L^?1 (C₀ = 5 μg L^?1).     

Health risk estimation due to carbon monoxide pollution at different spatial levels in Santiago, Chile

The Metropolitan Region of Santiago has been declared as nonattainment area for carbon monoxide (CO). The data was based on seven air monitoring stations that do not necessarily represent the real population exposure. Usually, a series of coefficients that relates the concentration of pollutants with health effects (betas of the concentration–response equations) are used. For the calculation of these betas, normally, a city average air pollution concentration is used; nevertheless, these betas do not necessarily represent the real risk of exposure that the inhabitants are experimented. For this reason, this study estimates two aspects that are unknown and that are necessary to policymakers in public health decisions. First, estimate the exposure at a lower spatial resolution level and, second, estimate a concentration–response curve that differs spatially and depends on the proportion of susceptible groups. We followed the Air Pollution and Health, European Approach/Spanish Multicenter Study on the Relationship Between Air Pollution and the Mortality methodology for the determination of the short-term health effect due to CO population exposure at the three spatial levels (city, zone, and county). We found that there are different risks in cardiorespiratory mortality in the group over 64 years old associated to an unequal exposure to CO in Santiago city. The higher risks are in counties located to the south–east of the city. These finding confirm the necessity to face, in a different way, the approach when evaluating control strategies and policy decision to control air pollution in Santiago.     

Three-dimensional data interpolation for environmental purpose: lead in contaminated soils in southern Brazil

Monitoring of heavy metal contamination plume in soils can be helpful in establishing strategies to minimize its hazardous impacts to the environment. The objective of this study was to apply a new approach of visualization, based on tridimensional (3D) images, of pseudo-total (extracted with concentrated acids) and exchangeable (extracted with 0.5 mol L^?1 Ca(NO₃)₂) lead (Pb) concentrations in soils of a mining and metallurgy area to determine the spatial distribution of this pollutant and to estimate the most contaminated soil volumes. Tridimensional images were obtained after interpolation of Pb concentrations of 171 soil samples (57 points × 3 depths) with regularized spline with tension in a 3D function version. The tridimensional visualization showed great potential of use in environmental studies and allowed to determine the spatial 3D distribution of Pb contamination plume in the area and to establish relationships with soil characteristics, landscape, and pollution sources. The most contaminated soil volumes (10,001 to 52,000 mg Pb kg^?1) occurred near the metallurgy factory. The main contamination sources were attributed to atmospheric emissions of particulate Pb through chimneys. The large soil volume estimated to be removed to industrial landfills or co-processing evidenced the difficulties related to this practice as a remediation strategy.     

Gender and community-oriented wildlife conservation: views from project supervisors in India

Many international agreements, such as the 1992 UN Convention on Biological Diversity, posit that successful community-oriented (community-based) wildlife conservation depends on partnerships with stakeholders of different class, ethnicity, and gender. Gender is of particular interest because it often relates to environmental use, attitudes, and knowledge and operates across other key categories. This study uses fieldwork, interviews, and a survey of 52 project heads in India to address two research questions: (1) How are gender issues viewed by supervisors of community-based wildlife conservation projects, in relation to their work? (2) What types of resources would be most useful to project heads seeking to promote gender equity through their conservation work? The results suggest that while there is widespread support for integrating gender equity issues into community-oriented wildlife conservation, many believe that gender may be a potentially distracting and secondary issue. Several reasons for the variation in views were identified including the following: the dearth of relevant empirical research about gender issues in wildlife conservation; ambiguities about the concept of gender itself; and a lack of opportunities to critically discuss the role of gender equity issues for conservation. These factors may contribute to a disconnection between international rhetoric and on-the-ground practice as it relates to gender and community-oriented wildlife conservation. Increased opportunities for professional capacity building among project supervisors and staff members, coupled with increased collaboration between social and natural scientists, will be important for strengthening the links between international conservation policy and on-the-ground practice.     

Pesticide distribution in an agricultural environment in Argentina

An assessment of the off-site migration of pesticides from agricultural activity into the environment in the Neuquen River Valley was performed. The aim of this study was to evaluate the distribution of pesticides in several compartments of a small agricultural sub-catchment. Soil, surface water, shallow groundwater and drift deposition were analyzed for pesticide residues. Results showed the presence of some pesticide residues in soil, surface water and shallow groundwater compartments. The highest detection frequencies in water (surface and subsurface) were found for azinphos-methyl and chlorpyrifos (>70%). In terms of concentration, the highest levels were observed in shallow groundwater for azinphos methyl (22.5 μg/L) and carbaryl (45.7 μg/L). In the soil, even before the application period had started, accumulation of residues was present. These residues increased during the period studied. Spray drift during pesticide application was found to be a significant pathway for the migration of pesticide residues in surface water, while leaching and preferential flows were the main transport routes contributing to subsurface contamination.     

Cr(VI) sorption by free and immobilised chromate-reducing bacterial cells in PVA–alginate matrix: equilibrium isotherms and kinetic studies

Chromate-resistant bacterial strain isolated from the soil of tannery was studied for Cr(VI) bioaccumulation in free and immobilised cells to evaluate its applicability in chromium removal from aqueous solution. Based on the comparative analysis of the 16S rRNA gene, and phenotypic and biochemical characterization, this strain was identified as Paenibacillus xylanilyticus MR12. Mechanism of Cr adsorption was also ascertained by chemical modifications of the bacterial biomass followed by Fourier transform infrared spectroscopy analysis of the cell wall constituents. The equilibrium biosorption analysed using isotherms (Langmuir, Freundlich and Dubinin–Redushkevich) and kinetics models (pseudo-first-order, second-order and Weber–Morris) revealed that the Langmuir model best correlated to experimental data, and Weber–Morris equation well described Cr(VI) biosorption kinetics. Polyvinyl alcohol alginate immobilised cells had the highest Cr(VI) removal efficiency than that of free cells and could also be reused four times for Cr(VI) removal. Complete reduction of chromate in simulated effluent containing Cu²⁺, Mg²⁺, Mn²⁺ and Zn²⁺ by immobilised cells, demonstrated potential applications of a novel immobilised bacterial strain MR12, as a vital bioresource in Cr(VI) bioremediation technology.     

Growth and Histopathological Effects of Chronic Exposition of Marine Pejerrey Odontesthes argentinensis Larvae to Petroleum Water-Soluble Fraction (WSF)

The water-soluble fraction (WSF) of petroleum contains a mixture of polycyclic aromatic hydrocarbons, volatile hydrocarbons, phenols, and heterocyclic compounds, considered deleterious to aquatic biota. Marine “pejerrey” Odontesthes argentinensis (Teleostei: Atherinopsidae) has a great commercial importance in local fisheries and a high potential for aquaculture. The aim of this study was to evaluate the histopathological effects in “pejerrey” larvae exposed to different concentrations of petroleum WSF. The chronic toxicity test was conducted with newly hatched larvae exposed for 21 days to sublethal concentrations of WSF (2.5, 5, 10, and 20 % of WSF), plus one control. Survival and growth were significantly lower in the highest concentration. Several histopathological changes were found in the gills (e.g., hyperplasia, aneurisms, edema, and necrosis), kidney (e.g., nuclear alterations, decrease in the hematopoietic cells), and liver (e.g., hypertrophy, karyorrhexis, and karyopyknosis). An index of branchial lesion was proposed to standardize gill lesions to different pollutants.