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91.
This study characterizes the persistence of human norovirus in Eastern oysters (Crassostrea virginica) held at different seawater temperatures. Oysters were contaminated with human norovirus GI.1 (Norwalk strain 8FIIa) by exposing them to virus-contaminated water at 15 °C, and subsequently holding them at 7, 15, and 25 °C for up to 6 weeks. Viral RNA was extracted from oyster tissue and hemocytes and quantitated by RT-qPCR. Norovirus was detected in hemocytes and oysters held at 7 and 15 °C for 6 weeks and in hemocytes and oysters held at 25 °C for up to 2 and 4 weeks, respectively. Results confirm that NoV is quite persistent within oysters and demonstrate that cooler water temperatures extend norovirus clearance times. This study suggests a need for substantial relay times to remove norovirus from contaminated shellfish and suggests that regulatory authorities should consider the effects of water temperature after a suspected episodic norovirus-contamination event. 相似文献
92.
Nicolas Nantel-Fortier Ann Letellier Virginie Lachapelle Philippe Fravalo Yvan L’Homme Julie Brassard 《Food and environmental virology》2016,8(4):296-304
Viral contamination along the production chain is a significant concern in both food safety and livestock health. Pigs have been reported to act as a reservoir for zoonotic viruses, sometimes emerging ones, and epidemiological studies have shown direct links between the consumption of uncooked pork offal and cases of hepatitis caused by the hepatitis E virus (HEV) genotype 3 in humans. The presence of HEV in swine herds has been reported, but its dissemination in pork production environments is still unknown. To investigate viral contamination sources in the swine industry, 452 environment and fecal samples, including samples from livestock transportation vehicles, were collected over a period of 11 months from ten farms and one slaughterhouse that together represent a single production network. Hepatitis E virus RNA was detected by nested RT-PCR in 32 samples from both inside and outside farm buildings, on trucks, and, mostly, from fomites collected in the slaughterhouse yard, such as on a utility vehicle. Phylogenetic analysis showed a wide diversity of HEV genotype 3 strains, similar to human and swine strains previously found. According to the results of this study, the movements of trucks and utility vehicles might play an important role in HEV dissemination on a slaughterhouse site and throughout an entire network. 相似文献
93.
Enrico Cappellini M. Thomas P. Gilbert Filippo Geuna Girolamo Fiorentino Allan Hall Jane Thomas-Oates Peter D. Ashton David A. Ashford Paul Arthur Paula F. Campos Johan Kool Eske Willerslev Matthew J. Collins 《Die Naturwissenschaften》2010,97(2):205-217
We report here the first integrated investigation of both ancient DNA and proteins in archaeobotanical samples: medieval grape (Vitis vinifera L.) seeds, preserved by anoxic waterlogging, from an early medieval (seventh–eighth century A.D.) Byzantine rural settlement in the Salento area (Lecce, Italy) and a late (fourteenth–fifteenth century A.D.) medieval site in York (England). Pyrolysis gas chromatography mass spectrometry documented good carbohydrate preservation, whilst amino acid analysis revealed approximately 90% loss of the original protein content. In the York sample, mass spectrometry-based sequencing identified several degraded ancient peptides. Nuclear microsatellite locus (VVS2, VVMD5, VVMD7, ZAG62 and ZAG79) analysis permitted a tentative comparison of the genetic profiles of both the ancient samples with the modern varieties. The ability to recover microsatellite DNA has potential to improve biomolecular analysis on ancient grape seeds from archaeological contexts. Although the investigation of five microsatellite loci cannot assign the ancient samples to any geographic region or modern cultivar, the results allow speculation that the material from York was not grown locally, whilst the remains from Supersano could represent a trace of contacts with the eastern Mediterranean. 相似文献
94.
Flower colour is an important signal used by flowering plants to attract pollinators. Many anthophilous insects have an innate colour preference that is displayed during their first foraging bouts and which could help them locate their first nectar reward. Nevertheless, learning capabilities allow insects to switch their colour preferences with experience and thus, to track variation in floral nectar availability. Manduca sexta, a crepuscular hawkmoth widely studied as a model system for sensory physiology and behaviour, visits mostly white, night-blooming flowers lacking UV reflectance throughout its range in the Americas. Nevertheless, the spectral sensitivity of the feeding behaviour of naïve moths shows a narrow peak around 450 nm wavelengths, suggesting an innate preference for the colour blue. Under more natural conditions (i.e. broader wavelength reflectance) than in previous studies, we used dual choice experiments with blue- and white-coloured feeders to investigate the innate preference of naïve moths and trained different groups to each colour to evaluate their learning capabilities. We confirmed the innate preference of M. sexta for blue and found that these moths were able to switch colour preferences after training experience. These results unequivocally demonstrate that M. sexta moths innately prefer blue when presented against white flower models and offer novel experimental evidence supporting the hypothesis that learning capabilities could be involved in their foraging preferences, including their widely observed attraction to white flowers in nature. 相似文献
95.
Huy Thanh Vo Tsuyoshi Imai Truc Thanh Ho Thanh-Loc Thi Dang Son Anh Hoang 《环境科学学报(英文版)》2015,36(10):38-47
Recently emerging disadvantages in conventional disinfection have heightened the need for finding a new solution. Developments in the use of high pressure carbon dioxide for food preservation and sterilization have led to a renewed interest in its applicability in wastewater treatment and water disinfection. Pressurized CO2 is one of the most investigated methods of antibacterial treatment and has been used extensively for decades to inhibit pathogens in dried food and liquid products. This study reviews the literature concerning the utility of CO2 as a disinfecting agent, and the pathogen inactivation mechanism of CO2 treatment is evaluated based on all available research. In this paper, it will be argued that the successful application and high effectiveness of CO2 treatment in liquid foods open a potential opportunity for its use in wastewater treatment and water disinfection. The findings from models with different operating conditions (pressure, temperature, microorganism, water content, media …) suggest that most microorganisms are successfully inhibited under CO2 treatment. It will also be shown that the bacterial deaths under CO2 treatment can be explained by many different mechanisms. Moreover, the findings in this study can help to address the recently emerging problems in water disinfection, such as disinfection by-products (resulting from chlorination or ozone treatment). 相似文献
96.
Waled Morsy El-Senousy Ahmed Mohammed El-Sayed Ragab Eman Mohammed Abd El Hamed Handak 《Food and environmental virology》2015,7(2):132-141
The objective of this study is to compare the prevalence of rotaviruses groups A and C in Egyptian children and aquatic environment. From 110 stool specimens of children with acute diarrhea and using RT-PCR, 35 samples (31.8 %) were positive for human rotavirus group A and 15 samples (13.6 %) were positive for human rotavirus group C. From 96 samples collected from Zenin wastewater treatment plant over a 2-year period (November 2009–October 2011) and using RT-PCR, rotavirus group A was detected in (4/24) 16.7 %, (5/24) 20.8 %, (4/24) 16.7 %, and (4/24) 16.7 %, while rotavirus group C was detected in (2/24) 8.3 %, (3/24) 12.5 %, (3/24) 12.5 %, and (0/24) 0 % in raw sewage, after primary sedimentation, after secondary sedimentation, and after final chlorination, respectively. Moreover, from 96 samples collected from El-Giza water treatment plant over a 2-year period (November 2009–October 2011), rotavirus group A was detected in (7/24) 29.2 %, (6/24) 25 %, (5/24) 20.8 %, and (3/24) 12.5 %, while rotavirus group C was detected in (3/24) 12.5 %, (1/24) 4.2 %, (1/24) 4.2 %, and (0/24) 0 % in raw Nile water, after sedimentation, after sand filtration, and after final chlorination, respectively. Using SYBR Green real-time RT-PCR, the number of human rotavirus group A genome or infectious units was higher than rotavirus group C. VP6 sequence analysis of the RT-PCR positive rotavirus group C samples revealed that four clinical specimens and three environmental samples showed similar sequences clustered with Moduganari/Human Nigerian strain AF 325806 with 98 % homology, and two clinical specimens and one environmental sample showed similar sequences clustered with Dhaka CB/Human Bangladesh strain AY 754826 with 97 % homology. 相似文献
97.
Recycling wastewater is becoming more common as communities around the world try to better control their water resources against an increased frequency of either prolonged droughts or intense flooding. For communities in coastal areas, wastewaters may contain elevated levels of bromide(Br~-) and iodide(I~-) from seawater intrusion or high mineral content of source waters. Disinfection of such wastewater is mandatory to prevent the spread of pathogens, however little is known about the toxicity of wastewater after disinfection in the presence of Br~-and I~-. In this study we compared the induction of chronic cytotoxicity in mammalian cells in samples of municipal secondary wastewater effluent amended with elevated levels of Br~-/I~-after disinfection by chlorine, chloramines or ozone to identify which disinfection process generated wastewater with the lowest level of adverse biological response. Chlorination increased mammalian cell cytotoxicity by 5times as compared to non-disinfected controls. Chloramination produced disinfected wastewater that expressed 6.3 times more cytotoxicity than the non-disinfected controls and was 1.3 times more cytotoxic than the chlorinated samples. Ozonation produced wastewater with cytotoxicity comparable to the non-disinfected controls and was at least 4times less cytotoxic than the chlorine disinfected wastewaters. These results indicate that compared to chlorination and chloramination, ozonation of wastewater with high Br~-/Ilevels yielded the lowest mammalian cell cytotoxicity, suggesting its potential as a more favorable method to disinfect wastewater with minimizing the biological toxicity in mind. 相似文献
98.
Kevin Shirley Eric Marland Jenna Cantrell Gregg Marland 《Mitigation and Adaptation Strategies for Global Change》2011,16(3):325-346
We recognize that carbon-containing products do not decay and release CO2 to the atmosphere instantaneously, but release that carbon over extended periods of time. For an initial production of a
stock of carbon-containing product, we can treat the release as a probability distribution covering the time over which that
release occurs. The probability distribution that models the carbon release predicts the amount of carbon that is released
as a function of time. The use of a probability distribution in accounting for the release of carbon to the atmosphere realizes
a fundamental shift from the idea that all carbon-containing products contribute to a single pool that decays in proportion
to the size of the stock. Viewing the release of carbon as a continuous probabilistic process introduces some theoretical
opportunities not available in the former paradigm by taking advantage of other fields where the use of probability distributions
has been prevalent for many decades. In particular, theories developed in the life insurance industry can guide the development
of pricing and payment structures for dealing with the costs associated with the oxidation and release of carbon. These costs
can arise from a number of proposed policies (cap and trade, carbon tax, social cost of carbon, etc), but in the end they
all result in there being a cost to releasing carbon to the atmosphere. If there is a cost to the emitter for CO2 emissions, payment for that cost will depend on both when the emissions actually occur and how payment is made. Here we outline
some of the pricing and payment structures that are possible which result from analogous theories in the life insurance industry.
This development not only provides useful constructs for valuing sequestered carbon, but highlights additional motivations
for employing a probability distribution approach to unify accounting methodologies for stocks of carbon containing products. 相似文献
99.
Shamama Afreen Nitasha Sharma Rajiv K. Chaturvedi Ranjith Gopalakrishnan N. H. Ravindranath 《Mitigation and Adaptation Strategies for Global Change》2011,16(2):177-197
Due to large scale afforestation programs and forest conservation legislations, India’s total forest area seems to have stabilized
or even increased. In spite of such efforts, forest fragmentation and degradation continues, with forests being subject to
increased pressure due to anthropogenic factors. Such fragmentation and degradation is leading to the forest cover to change
from very dense to moderately dense and open forest and 253 km2 of very dense forest has been converted to moderately dense forest, open forest, scrub and non-forest (during 2005–2007).
Similarly, there has been a degradation of 4,120 km2 of moderately dense forest to open forest, scrub and non-forest resulting in a net loss of 936 km2 of moderately dense forest. Additionally, 4,335 km2 of open forest have degraded to scrub and non-forest. Coupled with pressure due to anthropogenic factors, climate change
is likely to be an added stress on forests. Forest sector programs and policies are major factors that determine the status
of forests and potentially resilience to projected impacts of climate change. An attempt is made to review the forest policies
and programs and their implications for the status of forests and for vulnerability of forests to projected climate change.
The study concludes that forest conservation and development policies and programs need to be oriented to incorporate climate
change impacts, vulnerability and adaptation. 相似文献
100.
Charlotte Anne Nakakaawa Paul O. Vedeld Jens B. Aune 《Mitigation and Adaptation Strategies for Global Change》2011,16(1):25-62
Using a map overlay procedure in a Geographical Information System environment, we quantify and map major land use and land
cover (LULC) change patterns in Uganda period 1990–2005 and determine whether the transitions were random or systematic. The
analysis reveals that the most dominant systematic land use change processes were deforestation (woodland to subsistence farmland—3.32%);
forest degradation (woodland to bushland (4.01%) and grassland (4.08%) and bush/grassland conversion to cropland (5.5%) all
resulting in a net reduction in forests (6.1%). Applying an inductive approach based on logistic regression and trend analyses
of observed changes we analyzed key drivers of LULC change. Significant predictors of forest land use change included protection
status, market access, poverty, slope, soil quality and presence/absence of a stream network. Market access, poverty and population
all decreased the log odds of retaining forests. In addition, poverty also increased the likelihood of degradation. An increase
in slope decreased the likelihood of deforestation. Using the stock change and gain/loss approaches we estimated the change
in forest carbon stocks and emissions from deforestation and forest degradation. Results indicate a negligible increase in
forest carbon stocks (3,260 t C yr-1) in the period 1990–2005 when compared to the emissions due to deforestation and forest
degradation (2.67 million t C yr-1). In light of the dominant forest land use change patterns, the drivers and change in carbon
stocks, we discuss options which could be pursued to implement a future national REDD plus strategy which considers livelihood,
biodiversity and climate change mitigation objectives. 相似文献