TNT in Komposten und Flüssigkultur

The formation of aromatic amines was investigated using a summarized test (NEDA-test) during the composting of 2,4,6-trinitrotoluene (TNT) contaminated soil. In this test, the aromatic amines were diazolated and then coupled to N-1-Naphthyl-ethylenediamine-dihydrochloride (NEDA) to yield an azo dye which can be monitored photometrically. The test was calibrated for known TNT-metabolites with an active amine-group. Liquid samples from composting- and liquid-culture-experiments were analyzed by HPLC for these known metabolites. Moreover, the samples were monitored by the NEDA-test and the expected extinction of the TNT-metabolites found with amine function were extrapolated with the help of calibration curves. It was shown that substantial differences are obvious between the monitored and extrapolated values. After separation into polar and non-polar aromatic amines, it became clear that these differences are made by the polar aromatic amines. Polar aromatic amines, which are not detectable by presently available analytical tests, were generated during the composting of TNT-contaminated soils. Contaminated stagnant water, which was generated during anaerobization of a compost prephase, was treated aerobically for 70 days in a biofermenter. During this treatment TNT and its known metabolites were eliminated almost entirely. Simultaneously, the toxicity in the Lumis Tox-test decreased drastically. In striking contrast, the sum of aromatic amines decreased only to a minor extent. Moreover, the percentage of polar compounds from total amount of aromatic amines increased drastically from 48% to more than 95%. At present, the chemical identification of these polar compounds is still missing and is the object for further research.     

International trade in biofuels: an introduction to the special issue

Currently, many countries are establishing goals for substituting biofuels for fossil fuels. These goals usually foresee 5–10% substitution while today's production, in most countries, is far below 2%. Evidently, many countries will seek to meet their ambitious biofuel targets through imports. This global trade in biofuels, which is to some extent already taking place, will have a major impact not only on other commodity markets like vegetable oils or animal fodder but also on the global land use change and on environmental impacts. This special issue focuses on the relation between trading, policy making and sustainability impacts of biofuels. It demonstrates the strong but complex link between biofuels production and the global food market, it unveils policy measures as the main drivers for production and use of biofuels and it analyzes various sustainability indicators and certification schemes for biofuels with respect to minimizing the adverse effects of biofuels while maximizing the benefits of the future use of biofuels.     

Non-specific association between filamentous bacteria and fungus-growing ants

Fungus-growing ants and their fungal cultivar form a highly evolved mutualism that is negatively affected by the specialized parasitic fungus Escovopsis. Filamentous Pseudonocardia bacteria occurring on the cuticle of attine ants have been proposed to form a mutualistic interaction with these ants in which they are vertically transmitted (i.e. from parent to offspring colonies). Given a strictly vertical transmission of Pseudonocardia, the evolutionary theory predicts a reduced genetic variability of symbionts among ant lineages. The aim of this study was to verify whether actinomycetes, which occur on Acromyrmex octospinosus leaf-cutting ants, meet this expectation by comparing their genotypic variability with restriction fragment length polymorphisms. Multiple actinomycete strains could be isolated from both individual ant workers and colonies (one to seven strains per colony). The colony specificity of actinomycete communities was high: Only 15% of all strains were isolated from more than one colony, and just 5% were present in both populations investigated. Partial sequencing of 16S ribosomal deoxyribonucleic acid of two of the isolated strains assigned both of them to the genus Streptomyces. Actinomycetes could also be isolated from workers of the two non-attine ant species Myrmica rugulosa and Lasius flavus. Sixty-two percent of the strains derived from attine ants and 80% of the strains isolated from non-attine ants inhibited the growth of Escovopsis. Our data suggest that the association between attine ants and their actinomycete symbionts is less specific then previously thought. Soil-dwelling actinomycetes may have been dynamically recruited from the environment (horizontal transmission), probably reflecting an adaptation to a diverse community of microbial pathogens.     

Multicriteria prioritization and partial order in environmental sciences

The complexity of the present data-centric world finds its expression in the increasing number of multi-indicator systems. This has led to the development of multicriteria ranking systems based on partial orders. Order theory is a main pillar of structural mathematics. Partial orders help to reveal why an object of interest holds a certain ranking position and how much it is subject to change if a composite indicator is upgraded. Order theory helps to derive linear or weak orders without indicator weighting schemes. Hence, rankings obtained from decision support systems (DSS) which depend on many parameters beyond the data matrix can be checked and discrepancies can lead to examine the parameters of the DSS. Order theory helps discover association and implication structures derived from formal concept lattices. Association and implication networks among the attributes of the data matrix allow more insights into multi-indicator systems and lead to new hypotheses and motivate further research. Some new and innovative concepts, like separated subsets, antagonistic indicators, ranking stability fields are rendered. Separated subsets are the typical outcome of a partial order analysis; their identification leads to antagonistic indicators, which are responsible for the separatedness of object’s subsets. Numerical aggregation can be performed step-by-step and the question which values of a weight lead to an order inversion is of high interest. The concept of stability fields is one possible answer, discussed in this paper. After an outline of partial order theory some more specific theoretical results are shown, then we discuss the role of composite indicators in the light of partial order and give some examples of interesting applications of partial order. Finally examples are selected from real life case studies of watersheds, environmental performance evaluations, child well being, geographic and administrative regions and more.