Effect of photoperiod and temperature on testicular regression in Channa punctatus

In Channa punctatus testicular regression commonly observed during spawning and postspawing phases of reproductive cycle. In the present study testicular regression was frequently noticed in fish maintained under both long photoperiod-warm temperature (LD 16 : 8-30 degrees C) and short photoperiod-warm temperature (LD 8 : 16-30 degrees C) regimes. Testicular regression was characterized by distortion of cellular boundary of lobules and formation of collagenous capsules containing degenerating germ cells, blood cells and colloidal mass within the lobules. The magnitude of testicular regression was more in fish exposed to short photoperiod regime (R-73.33%, SP-41.67%) than long photoperiod regime (R-50.83%, SP-19.16%) and control group (R-20.83%, SP-16.67%) in both resting (R) and spawning (SP) phases. Further the frequency of testicular regression during resting phase was 73.33% (short photoperiod), 50.83% (long photoperiod) and 20.83% (control) whereas during spawning phase was 41.67% (short photoperiod) 19.16% (long photoperiod) and 16.67% (control). In the present study occurrence of more testicular regression during resting phase than spawning phase may be due to change in the endogenous condition of the fish.     

Abundance and diversity of the phyllosphere bacterial communities of Mediterranean perennial plants that differ in leaf chemistry

Summary.  We studied the epiphytic bacterial communities of the summer leaves of eight perennial species naturally occurring in a Mediterranean ecosystem. The species differ in essential-oil content (from rich in essential oil to non-producers) and composition, and also in life form (from herbaceous species to tall shrubs). We compared the epiphytic bacterial communities on the basis of (i) their abundance, (ii) their metabolic profile (derived by use of the BIOLOG Ecoplate system) and (iii) richness and diversity of substrates that they use, as a measure of functional diversity. Among all species, the aromatic Melissa officinalis was the most abundantly colonized. The bacterial communities on the leaves of the aromatic Myrtus communis, Calamintha nepeta and Melissa officinalis, and also of Cistus incanus catabolized all 31 substrates offered; those on the evergreen-sclerophyllous species, Arbutus unedo and Quercus coccifera, catabolized only 14 and 17 substrates, respectively. Carbohydrates were consistently used abundantly by all communities, whereas carboxylic acids were most variably used. On average, the group of aromatic plants scored higher regarding bacterial abundance, and richness and diversity of substrates used by the bacterial communities on their leaves; the lowest values for both substrate-use indices were recorded in A. unedo. Bacterial abundance or richness or diversity of substrates used did not vary with leaf oil content. Abundance was positively correlated with both substrate-use indices. Results support claims that the antimicrobial effects of essential oils are not exerted so much under natural conditions as reports based on biassays with pathogens usually show. Although essential oils play a part in the microbial colonization of the phyllosphere, it is not likely that inhibition of phyllosphere bacteria is essential oils’ primary role, at least in the Mediterranean environment.     

Population structure and growth of Donax trunculus (Bivalvia: Donacidae) in the western Mediterranean

A comparison of shell growth in Donax trunculus (collected between 1988 and 1990 of Cullera, Spain) has been carried out using an analysis of cohort progression in monthly length frequency distributions, hyaline surface shell growth rings and internal microgrowth bands. In the Mediterranean there are two periods of recruitment of D. trunculus, one in the summer (July to September) and the other in winter (December to February). Clams recruited to the population in winter display a clear cessation in shell growth during the following summer which may possibly be correlated with spawning, whereas individuals of the summer recruited cohort show no growth cessation the following summer and continue to deposit shell during this period. The normally opaque shell of D.trunculus reveals the presence of translucent hyaline growth rings when the shells are backlit by a strong light source, and these have been shown to be laid down in the shell during summer months. Formation of a hyaline ring is accompanied by a narrowing of the microgrowth patterns present in shell sections. Both the hyaline rings and the length frequency distributions have been used to determine the age and growth rate of D. trunculus.     

Modeling seed dispersal distances: implications for transgenic Pinus taeda.

Predicting forest-tree seed dispersal across a landscape is useful for estimating gene flow from genetically engineered (GE) or transgenic trees. The question of biocontainment has yet to be resolved, although field-trial permits for transgenic forest trees are on the rise. Most current field trials in the United States occur in the Southeast where Pinus taeda L., an indigenous species, is the major timber commodity. Seed dispersal distances were simulated using a model where the major determinants were: (1) forest canopy height at seed release, (2) terminal velocity of the seeds, (3) absolute seed release, and (4) turbulent-flow statistics, all of which were measured or determined within a P. taeda plantation established from seeds collected from wild forest-tree stands at the Duke Forest near Durham, North Carolina, USA. In plantations aged 16 and 25 years our model results showed that most of the seeds fell within local-neighborhood dispersal distances, with estimates ranging from 0.05 to 0.14 km from the source. A fraction of seeds was uplifted above the forest canopy and moved via the long-distance dispersal (LDD) process as far as 11.9-33.7 km. Out of 10(5) seeds produced per hectare per year, roughly 440 seeds were predicted to be uplifted by vertical eddies above the forest canopy and transported via LDD. Of these, 70 seeds/ha traveled distances in excess of 1 km from the source, a distance too great to serve as a biocontainment zone. The probability of LDD occurrence of transgenic conifer seeds at distances exceeding 1 km approached 100%.     

Integrated shoreline management and tourism development on the cross-border World Heritage Site: A case study from the Curonian spit (Lithuania/Russia)

The varied and very dynamic landscape with a high biological diversity is a distinctive feature of the Curonian spit at the regional scale. Throughout the 20th century the main morphodynamic trend in the littoral of the Curonian spit was shoreline grading on the lagoon side, whereas on the marine side the increasing erosion at the foot of the spit, and increasing accretion at the head of the spit prevailed. The results of a discriminant analysis (Wilkes’ λ = 0.001626 and F = 29.267 when p < 0.001) show that sites with prevailing erosion, accretion and sediment input from the drifting dunes form regular inter-related spatial structures in the littoral with distinctive resulting discontinuities of the sediment drift along the lagoon coast. Dune littoral cells are characterized by Aeolian sediment input and distribution ‘down-drift’, (usually northwards) from the source. The most likely changes in the current development trends of the lagoon shore zone of the Curonian spit are related to expected climate changes and further slowing down of the dune drift. The probability of storms and ice-drift events, and their impact on coasts is expected to increase as a result of climate change. The dune advance will gradually slow down, and with it, the sand input to the coastal zone will decline. In this paper, we define integrated shoreline management as a system of long-term shoreline management measures, which is based on a littoral cell approach and aimed at harmonizing human activity in the coastal zone with the natural development of the shoreline. We propose an integrated management program for the lagoon shoreline of the Curonian spit, which is site-specific for each littoral cell as a coastal management unit. Drifting dunes and seaside beaches are the natural amenities, which are best known and best appreciated on the Curonian spit by 49% of the respondents representing the total Lithuanian population. A responsible tourism development should be considered as the key means for proper appreciation of the drifting dunes and natural coasts by society, which means to acknowledge and cherish the aesthetic and conservation values of dune and coastal landscapes of the Curonian spit as a World Heritage Site. There are at least two pre-conditions for this: (1) to provide visitors with sufficient information about diverse values and functions of the Curonian dunes and coasts within a broader regional and global heritage conservation context; (2) to enable tourists to enjoy the most impressive dune and coastal landscapes at close range. In this paper we propose to encourage active dune tourism, to reintroduce grazing into the Curonian dune areas, to restore and to preserve the most impressive landscapes of the highest white drifting dunes by bringing the blown out sand from the leeward foot of the dunes back to the crest artificially.     

Seasonal ovarian cycle in freshwater teleost, Labeo rohita (Ham.) in Tarai region of Uttaranchal

Seasonal ovarian cycle, based on histophysiological changes, and correlative variations in liver were studied in an Indian major carp, Labeo rohita reared in captivity under ambient environmental conditions in Tarai region of Uttaranchal. The oocyte features used for marking ovarian cycle included nuclear-ooplasmic ratio, yolk vesicle incorporation into ooplasm, number and size of nucleolus and differentiation of follicular layer. Based on these features the ovarian cycle in L. rohita was divided into five phases i.e. resting, preparatory, pre-spawning, spawning and post-spawning/regression. The resting and preparatory phases were characterized by the dominating presence of chromatin-nucleolar and yolk-vesicle stage oocytes. The pre-spawning phase ovary was marked by the prevalence of yolk globule stage oocytes having cortical alveoli and differentiating follicular wall. The oocytes containing densely packed yolk-granules and large masses of yolk vesicles and also exhibiting germinal vesicle movement (GVM) signified the ovary of spawning phase. The ovary in regression phase contained pre-ovulatory atretic oocytes and significant number of oogonia. The histophysiological changes in liver showing augmented biosynthetic activity during preparatory and pre-spawning phases seemed well correlated with the gonadal development. The increasing water temperature and day-length apparently was favourable for the gonadal development in L. rohita.     

Cleanup Options for Navajo Abandoned Uranium Mines

This article summarizes the current status of known contamination arising from abandoned uranium mines (AUMs) within the Navajo Nation and examines the options for addressing the elevated risks to health that AUM waste poses to the people of the Navajo Nation. More specifically, this article provides the following:

1. An overview of past uranium mining conducted on the Navajo Nation, the current status of AUM waste within the Navajo Nation, and the human health risks associated with uranium exposure.
2. A discussion of Navajo Fundamental Law, the Diné (the Navajo People) Uranium Remediation Advisory Commission, and the Navajo Nation Department of Justice's position regarding institutional controls and cleanup of AUMs.
3. A summary of cleanup actions taken to date to address AUM waste and the lessons learned from such actions.
4. Options for cleanup of AUM waste consistent with Diné Fundamental Law, the Diné Natural Resources Protection Act of 2005, and the Comprehensive Environmental Response, Compensation, and Liability Act of 1980 (CERCLA).

The information summarized in this article was previously presented as an “Initial White Paper on Cleanup Options for Navajo Abandoned Uranium Mines,” prepared with participation from multiple stakeholders including the Navajo Nation Environmental Protection Agency (NNEPA), the Navajo Nation Department of Justice (NNDOJ), and the United States Environmental Protection Agency (US EPA). ©2016 Wiley Periodicals, Inc.