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141.
Renata de Lima Anderson do Espirito Santo Pereira Raquel Martins Porto Leonardo Fernandes Fraceto 《Journal of Polymers and the Environment》2011,19(1):196-202
This work reports on an analysis of the cyto- and genotoxicity of poly(lactide-co-glycolide) polymer nanoparticles, in an
attempt to evaluate their mutagenic effects. Fibroblast (3T3) and human lymphocyte cell cultures were exposed to solutions
containing three different concentrations of nanoparticles (5.4, 54 and 540 μg/mL, polymer mass/volume of solution). The nanoparticles
were characterized in terms of their hydrodynamic diameters, zeta potentials and polydispersity indices. The morphology of
the particles was determined by atomic force microscopy. The PLGA nanospheres presented a size of 95 nm, a zeta potential
of −20 mV and a spherical morphology. Cellular viability assays using fibroblast cells showed no significant alterations compared
with the negative control. A cytogenetic analysis of human lymphocyte cells showed no significant changes in the mitotic index
in relation to the control, indicating that in the concentration range tested, the particles used in the experimental models
did not present cyto- or genotoxicity. For the tests conducted in this work we can conclude that biodegradable and biocompatible
PLGA nanospheres are not toxic in the cell cultures tested (fibroblast and lymphocyte cells) and in the range of concentrations
employed. The results provide new information concerning the toxic effects of particles produced using PLGA. 相似文献
142.
Bandala ER Andres-Octaviano J Pastrana P Torres LG 《Journal of environmental science and health. Part. B》2006,41(5):553-569
Degradation of aldrin (1,2,3,4,10,10-Hexachloro-1,4,4a,5,8,8a-hexahydro-1,4:5-8-dimethanonaphthalene), heptachlor (1H-1,4,5,6,7,8,8-heptachloro-3a,4,7,7a-tetrahydro-4,7-methano indene), dieldrin (1aalpha,2beta,2aalpha,3beta,6beta,6aalpha,7beta,7aalpha)-3,4,5,6,9,9-Hexachloro-1a,2,2a,3,6,6a,7,7a-octahydro-2,7:3,6-d-methanonaphtha[2,3-b]oxirene, and heptachlor epoxide (1aalpha, 1bbeta,2alpha,5alpha,5alphabeta,6beta,6aalpha-2,3,4,5,6,7,7-Heptachloro-1a,1b,5,5a,6,6a-hexahydro-2,5-methano-2H-inden[1,2-b]-oxirene) was tested using free cultures of Pseudomonas fluorescens under controlled conditions. Pesticide concentrations were monitored by gas chromatography during 120 h. Percentages of degradation and biodegradation rates (BDR) were calculated. Data showed a trend suggesting a relation between chemical structure and degradability. Degradation kinetics for each pesticide tested showed that the highest degradation rates were found in the first 24 h. Kinetics data were adjusted to an empirical equation in order to predict their behavior, and the correlation coefficients obtained were satisfactory. Gas chromatography/mass spectrometry (GC/MS) analysis of the final extracts allowed the identification of chlordene and monodechlorodieldrin, which have been reported as final metabolite produced in the biodegradation of this kind of compounds. Regarding adsorption of pesticides on activated vegetal carbon, we concluded that removal efficiencies between 95.45 and 97.18% can be reached, depending on the pesticide and the carbon dose applied. The values for K from the Freundlich equation were quite similar for the four pesticides (between 1.0001 and 1.04), whereas the n values were quite different for each pesticide in the following order of affinity: dieldrin > aldrin > heptachlor epoxide > heptachlor. Equilibrium times, very important for scaling up the process, were between 43 min and 1 h, for the heptachlor epoxide and the heptachlor, respectively. 相似文献
143.
The distribution of trace metals (Co, Cu, Fe, Mn, Ni, Pb and Zn) was investigated during a year (1994-95) in surface and core sediment samples and in the oyster (Crassotrea rhizophorae) from Bluefields Bay, Nicaragua. the aim was to assess the arthropogenic impact of potential pollutant sources, mainly Bluefields City, since domestic waste waters are discharged directly or by infiltration to the bay. Lyophilised samples were submitted to different acid digestion methods and analysed by flame atomic absorption spectrophotometry. the results showed highest contents for copper, lead and zinc near Bluefields City, with an increase in the affected area in the rainy season that is generated by greater city run off. Metal contents in oysters do not show the same distribution pattern than in sediments and were similar to those from other areas without reflecting pollution levels. 相似文献
144.
145.
Richard G. Ryall David Callen Robert Cocciolone Amra Duvnjak Renata Esca Niki Frantzis Elizabeth M. Gjerde Eric A. Haan Trudy Hocking Grant Sutherland David W. Thomas Fiona Webb 《黑龙江环境通报》2001,21(7):553-557
Of the 65 328 pregnancies of South Australian mothers screened by the South Australian Maternal Serum Antenatal Screening (SAMSAS) Programme between 1 January 1991 and 31 December 1997, 3431 (5.25%) were declared at increased risk of fetal Down syndrome. Fetal or neonatal karyotype was determined in 2737/3431 (79.8%) of these pregnancies, including 16 with early fetal loss. Interrogation of the database of the South Australian Neonatal Screening Service showed 643 live-born infants whose phenotype was not subsequently questioned among the 694 pregnancies whose karyotype was not determined. Of the remaining 51/3431 pregnancies, 19 ended in early fetal loss without karyotyping and no newborn screening or other records could be found for 32 cases. The 129 instances of abnormal karyotype found were Down syndrome (84), trisomy 18 (four), trisomy 13 (three), triploidy (two), female sex chromosome aneuploidy (six) and male sex chromosome aneuploidy (five), inherited balanced rearrangements (19), mosaic or de novo balanced abnormalities (four) and unbalanced karyotypes (two). In the pregnancies declared at increased risk of fetal Down syndrome, only the karyotype for Down syndrome occurred with a frequency greater than that expected for the general, pregnant population. Copyright © 2001 John Wiley & Sons, Ltd. 相似文献
146.
Prado Tatiana Barbosa Mikaela Renata Funada Araújo Ronalda Silva Garcia Suzi Cristina Melo Adalgisa Jesus Galvani Ana Tereza Brandão Carlos Jesus Silva Renan Lourenço Oliveira Sato Maria Inês Zanoli 《Food and environmental virology》2021,13(4):520-527
Food and Environmental Virology - Hepatitis A virus (HAV) is the major cause of enterically transmitted infectious hepatitis. Between 2016 and 2017, the number of confirmed cases of hepatitis A... 相似文献
147.
Electrona antarctica and Bathylagus antarcticus are dominant fishes in the Southern Ocean pelagic ecosystem with disparate life histories, making them excellent subjects
for comparative studies. Twenty-one indices of nutritional condition were compared in both species by observing changes in
each index as it varied spatially and temporally in association with the marginal ice-zone bloom in the northwestern Weddell
Sea. E. antarctica and B. antarcticus exhibited very different patterns of response to the bloom. Nutritional condition increased in 11 of 21 measures in E. antarctica, suggesting that it was in poorer condition at ice-covered stations and in greatly improved condition in post-bloom, open-water
stations. The data, combined with a few observations of larvae, indicate that E. antarctica increases feeding before the spring bloom, rapidly building stores of lipid, which are probably used for spawning. In contrast,
only three measures of condition were variable in B. antarcticus, and lipid actually decreased from ice-covered to open water. RNA:DNA values increased in open water, coinciding with an
increase in food volume present in their guts. These observations, coupled with observations of larvae in ice-covered stations,
suggest that B. antarcticus possesses sufficient energetic stores to spawn prior to the bloom, so that larvae are able to fully utilize available resources
associated with the spring bloom. Increased somatic growth, as indicated by RNA:DNA, may not begin until later, when the bloom
was beginning to decline. Earlier work, which suggested that the deeper living, non-migrating species, like B. antarcticus, would not be affected by the bloom until after shallower, migratory species, like E. antarctica, is supported. Of the assays tested, RNA:DNA appeared to be the most sensitive. The combined assessment of many measures
including biochemical, compositional, dietary, and age estimates from single specimens is possible if the samples are treated
with sufficient care. The reliance upon a single measure to help interpret the ecology of a species, especially in nekton-sized
species, is not as effective as techniques used in combination.
Received: 12 January 2000 / Accepted: 16 August 2000 相似文献
148.
Andreas Wade Gintaras Denafas Viktoras Racys Ingrida Rimaityte Renata Povilaityte 《Waste management & research》2006,24(1):27-36
The main purpose of this study was to carry out a retrospective analysis of solid waste generation in Kaunas city from 1994 to 2003 and to calculate the theoretical waste generation in the future. This paper also presents measurements of the annual variations of waste amounts and calculations on a theoretical waste incineration facility. Two waste treatment scenarios were considered. Scenario A: waste deposit at the landfill with 'implementation of domestic waste separation and recycling'. Scenario B: waste removal to a landfill with 'multi-treatment: household separation, recycling and energetic recovery'. Three levels of waste treatment were proposed. The first level was implementation of the recycling system, which included household waste separation. The next step involved mechanical-biological treatment. The third level was the construction and operation of a new waste incineration plant in Kaunas. Two sites in Kaunas city were proposed; however, more detailed analysis, including the economic factors, will need to be done. 相似文献
149.
g -glucosidase and alkaline phosphatase, two ectoenzymes involved in the microbial decomposition of polysaccharides and organic phosphates, were evaluated in water samples collected from two areas of the Northern Adriatic Sea during the multidisciplinary Prisma II research project. The distribution of g -glucosidase and alkaline phosphatase is reported together with that of leucine aminopeptidase, an enzyme involved in the degradation of protein compounds. The data obtained showed the prevalence of g -glucosidase in summer months in the northern area, located in front of the Po delta, while in winter it is higher in the southern area (in front of Ancona). Phosphatase activity during February, 1998 had a maximum of 107 r nml m 1 h m 1 at a coastal station in the northern area; this enzyme appeared to be mainly associated with phytoplankton because the increase in alkaline phosphatase was associated with the increase of phytoplankton biomass, measured by chlorophyll a content. The relations between the microbial activities and environmental parameters are discussed. 相似文献
150.
Rosabruna La Ferla Renata Zaccone Maurizio Azzaro Gabriella Caruso 《Chemistry and Ecology》2013,29(1-2):75-84
The carbon transfer through the microbial community in two areas of the Northern Adriatic Sea was estimated by proteolytic and respiratory activities during four oceanographic surveys carried out in June, 1996, 1997 and February, 1997, 1998. In front of the Po Delta (area A), the mean rates of proteolytic activity range from 4.9 to 9.9 r µg r C r h r l; near Ancona (area B), they range from 3.1 to 7.6 r µg r C r h r l. Respiratory rates vary between 0.19 and 2.29 and between 0.24 and 1.40 r µg r C r h r l in areas A and B, respectively. In general, high rates occur in the surface layers, within the first 10 r m of depth. In area A, proteolytic and respiratory rates undergo seasonal course, with high activity in warm periods. In area B, respiration and bacterioplankton abundance increase from the first to the second year, whilst proteolytic activity decreases. The sequence of metabolic steps in the carbon transfer within the bacteria, from the biotic vs . the abiotic compartment, was drawn in order to define the actual role of bacterial biomass in the biogeochemical fluxes in an ecosystem which often suffers distrophic crises. Respiratory turnover rates, in the upper 10 r m depth, reach low values in cold periods and high values in June, 1997. The carbon transfer versus mineralization flows better in the summer period, in particular in June, 1997. However, the bacterial growth efficiency ranges from 17 to 38% in area A and from 13 to 44% in area B with highest values in February, 1997, when bacteria contribute in a relevant way to the overall respiration. 相似文献