波罗的海鱼类和甲壳类的毒性物质及繁殖失调

在波罗的海生态系统中,由于毒性物质(例如DDT,PCB)的生物积累作用,在食物链上层捕食者(如海豹和食鱼鸟类)中已出现了繁殖失调.在过去25年间繁殖失调还影响到鱼类.然而,还没有强有力的证据说明这些问题是有毒物质引起的.更确切地说,这种失调似乎是由两种以上生物或生物的因素共同引起的.杀死鲑鱼和鳟鱼幼体的M74综合症,是由于缺乏硫胺素(维生素B1).许多因素可以导致维生素B1缺乏,包括鲑鱼的食物和卤代有机化合物.鳕鱼不生M74病,其资源得不到恢复是由于繁殖区域溶解氧缺乏和过度捕捞.肉类加工厂排放物中的毒性物质,而不是机械作用和活性物质,抑制了生殖腺的发育.肉类加工厂所在海域的外侧鱼类资源缺乏可以解释为幼鱼缺乏食物,而不是繁殖失败.有关波罗的海甲壳类繁殖失调的资料很少.大部分资料都是关于端足类Monoporeoaaffnis的研究,该种生物已被用于监测计划.该种生物的繁殖失调现象例如胚胎畸形、死亡,以及雌雄性不同时成熟等已有报道.     

Sources of variation in herbivore preference: among-individual and past diet effects on amphipod host choice

Understanding which factors affect the feeding preferences of herbivores is essential for predicting the effects of herbivores on plant assemblages and the evolution of plant–herbivore interactions. Most studies of marine herbivory have focussed on the plant traits that determine preferences (especially secondary metabolites), while few studies have considered how preferences may vary among individual herbivores due to genetic or environmental sources of variation. Such intraspecific variation is essential for evolutionary change in preference behaviour and may alter the outcome of plant–herbivore interactions. In an abundant marine herbivore, we determined the relative importance of among-individual and environmental effects on preferences for three host algae of varying quality. Repeated preference assays were conducted with the amphipod Peramphithoe parmerong and three of its brown algal hosts: Sargassum linearifolium, S. vestitum and Padina crassa. We found no evidence that preference varied among individuals, thus constraining the ability of natural selection to promote increased specialisation on high-quality S. linearifolium. Most of the variation in preference occurred within individuals, with amphipod preferences strongly influenced by past diet. The increased tendency for amphipods to select alternate hosts to that on which they had been recently feeding indicates that amphipods are actively seeking mixed diets. Such a feeding strategy provides an explanation for the persistence of this herbivore on hosts in the field that support poor growth and survival if consumed alone. The effects of past diet indicate that herbivore preferences are a function of herbivore history in addition to plant traits and are likely to vary with the availability of algae in space and time.     

Sponge-specific bacterial symbionts in the Caribbean sponge, Chondrilla nucula (Demospongiae, Chondrosida)

Marine sponges harbor dense and highly diverse bacterial communities, and some percentage of the microflora appears to be specialized for the sponge habitat. Bacterial diversity was examined in Chondrilla nucula Schmidt to test the hypothesis that some subset of sponge symbiont communities is highly similar regardless of the species of host or habitat requirements of the host. C. nucula was collected from a mangrove channel on Lower Matcumbe Key in the Florida Keys (25°53′N; 80°42′W) in August 1999. Domain-specific universal bacterial primers were used to amplify the 16S rDNA gene from genomic DNA that had been extracted from sponges and the surrounding water. An RFLP technique was used to assess diversity of sponge-associated and environmental bacterial communities. The clone library from C. nucula contained 21 operational taxonomic units (OTUs). None of the 53 OTUs from adjacent water samples were found in the C. nucula library indicating that a distinct community was present in the sponge. Sequence analysis indicated that C. nucula harbors a microbial community as diverse as the microbes from other sponges in different habitats around the world. Phylogenetic analysis placed several C. nucula clones in clades dominated by bacteria that appear to be sponge specialists (e.g., Acidobacteria, Bacteroidetes, and Cyanobacteria). Proportional representation of major bacterial taxonomic groups represented in symbiont communities was compared as a function of geographic location of sponge hosts. This study supports the hypothesis that sponges from different oceans existing in dissimilar habitats harbor closely related bacteria that are distinct from other bacterial lineages and appear specialized for residing within sponges.     

Rapid enzyme immunoassays for the detection of carbaryl and methoprene in grains

In order for grain handlers and traders to reliably estimate residues of grain protectants in the field, antibody-based rapid tests were developed for carbaryl (1-naphthyl methylcarbamate) and methoprene [isopropyl (E,E)-(RS)-11-methoxy-3,7,11-trimethyldodeca-2,4-dienoate]. To complement the rapid analysis, a simple and rapid extraction technique was developed. In these tests, a pesticide-containing methanol extract of the grain sample and an enzyme-labeled component are added to precoated strips. After a brief incubation, the strips are washed and a substrate/chromogen for the enzyme is added. The color developed is stopped by acidification and the results are read either by eye or in a portable field photometer. The overall test time is under 20 minutes. For carbaryl, the test had a limit of detection of 4.5 ppb (1.1 ppm in grain), while the methoprene test had a limit of detection of 4 ppb (1 ppm in grain) based on the lower datum point, which is 15% inhibition, in the standard curves. Both assays can be used as a screening test for carbaryl and methoprene in animal feed grains.     

Yolk androgen deposition as a compensatory strategy

The deposition of androgens into the yolks of eggs can have long-lasting effects on the growth and development of young birds. It has been proposed that female birds and reptiles deposit yolk androgens according to the differential allocation hypothesis (DAH), which posits the allocation of more resources to offspring sired by more attractive, higher-quality males. We examined deposition patterns of yolk androgens in relation to mate attractiveness in the house finch Carpodacus mexicanus. Contrary to the predictions of the DAH, female house finches deposited significantly more androgens into eggs sired by less attractive males. We propose that, rather than serving as resources, androgens are used as mediators in a compensatory distribution strategy, enabling females to improve the quality of young produced with less attractive males.     

Critical aspects of fluorescent age-pigment methodologies: Modification for accurate analysis and age assessments in aquatic organisms

Fluorescent age-pigment (FAP) quantification has recently been proposed as a means of estimating age in aquatic vertebrates and invertebrates. However, various aspects related to currently adopted procedures remain untested and a distinct relationship between FAP accumulation and chronological age has yet to be established. The present study was undertaken to critically evaluate the effects of specimen and extract handling procedures (including temperature, time, ultrasonication, and solvent systems) on the expression of native FAP fluorescence using the post-mitotic tissues of laboratoryreared specimens of the teleostOreochromis mossambicus as model systems. FAP-like fluorophores increased in vitro in brain, heart, and muscle tissues and their extracts with increased storage temperature (–20°C and above) and time. Sonification of homogenates greatly enhanced this effect and generated other non-native fluorophores in sample solution. Fluorescence assay temperature also affected expression of results, and aqueous-phase extracts, used in previous studies, were found to contain large amounts of fluorescent flavin contaminants. Using modifications of the above procedures, age-related patterns of accumulation were subsequently examined inO. mossambicus brain and, for the first time, positive correlations between chronological age and whole organ as well as weight-specific FAP concentration were validated. The significance of these findings are discussed in relation to previous FAP studies.     

Activity,track and speed of movement of the crab Scylla serrata in an estuary

Ultrasonic transmitters were used to track the movements of the crab Scylla serrata (Forskal) over 24 h periods in the Kowie estuary, South Africa. Laboratory experiments using infra-red time-lapse photography to record activity indicated that the transmitters did not affect duration of emergence, amount of movement or feeding. In the estuary, S. serrata was active on average for 13 h. out of 24 h, most activity was at night. The distance moved per night by continuously tracked crabs averaged 461 m, but ranged between 219 and 910 m. Most movement was slow, modal speed was 10 to 19 m h^-1. Slow movements were independent of direction of current and are assumed to be related to use of contact chemoreception for location of prey. About one-seventh of movements were faster than 70 m h^-1; these were most frequently against the current and may be related to olfactory location of food. The crabs did not occupy a distinct territory, but tended to remain in the same general area although they were capable of moving at least 800 m along the length of the estuary at night.     

The effect of rearing environment on blue structural coloration of eastern bluebirds (<Emphasis Type="Italic">Sialia sialis</Emphasis>)

We used a brood-size manipulation to test the effect of rearing environment on structural coloration of feathers grown by eastern bluebird (Sialia sialis) nestlings. Ultraviolet (UV)-blue structural coloration has been shown to be sexually selected in this species. Our experimental design took advantage of the growth of UV-blue wing feathers in nestlings that are retained as part of the first nuptial plumage. We cross-fostered nestlings to create enlarged and reduced broods with the purpose of manipulating parental feeding rates and measured the effect on nestling growth and plumage coloration. Brood size influenced feeding rates to offspring, but the effect varied with season. In general, male nestlings reared in reduced broods were fed more often, weighed more, and displayed brighter structural plumage compared to nestlings reared in enlarged broods. Female nestlings appeared to experience less adverse affects of brood enlargement, and we did not detect an effect of brood-size manipulation on the plumage coloration of female nestlings. Measures of plumage coloration in both males and females, however, were correlated to hatching date and nestling mass during feather development. These data provide empirical evidence that environmental quality can influence the development of the blue structural coloration of feathers and that males may be more sensitive to environmental fluctuations than females.     

Denitrification: its importance in a river draining an intensively cropped watershed

Nitrogen transport was studied during summer low flows in a 20-km reach of the Nottawasaga River which drains an intensively cropped sand plain which has an underlying shallow water-table aquifer. Nitrogen inputs to the river were measured on days in May to October of 1977-81. These data indicated that about 38% of the daily nitrate input entered the river through ground water. The magnitude of this input is a consequence of widespread contamination of the shallow aquifer by nitrogen fertilizer. Ground water entering the river from springs and seeps near fertilized fields frequently contained more than 10 mg 1^?1 of NO₃-N. Mass balance studies of nitrogen transport in the river revealed an average daily nitraof 46 ± 23 kg N. This rate of nitrate removal represented about 40% of the ground water input to the river from the sand plain. Analysis of a mass balance for total Kjeldahl nitrogen revealed an essentially balanced budget, whereas chloride showed a small daily gain of about 5%. Laboratory experiments involving the incubation of stream sediment cores and the use of the acetylene block technique suggested that the bulk of the nitrate loss during river transport was caused by denitrification in bottom sediments.     

Aggregation of Adenovirus 2 in Source Water and Impacts on Disinfection by Chlorine

It is generally accepted that viral particles in source water are likely to be found as aggregates attached to other particles. For this reason, it is important to investigate the disinfection efficacy of chlorine on aggregated viruses. A method to produce adenovirus particle aggregation was developed for this study. Negative stain electron microscopy was used to measure aggregation before and after addition of virus particles to surface water at different pH and specific conductance levels. The impact of aggregation on the efficacy of chlorine disinfection was also examined. Disinfection experiments with human adenovirus 2 (HAdV2) in source water were conducted using 0.2 mg/L free chlorine at 5 °C. Aggregation of HAdV2 in source water (≥3 aggregated particles) remained higher at higher specific conductance and pH levels. However, aggregation was highly variable, with the percentage of particles present in aggregates ranging from 43 to 71 %. Upon addition into source water, the aggregation percentage dropped dramatically. On average, chlorination CT values (chlorine concentration in mg/L × time in min) for 3-log₁₀ inactivation of aggregated HAdV2 were up to three times higher than those for dispersed HAdV2, indicating that aggregation reduced the disinfection rate. This information can be used by water utilities and regulators to guide decision making regarding disinfection of viruses in water.