Local adaptation of immunity against a trematode parasite in marine amphipod populations

Resources allocated to defence against parasites are not available for investment in other functions such as growth or reproduction, resulting in trade-offs between different components of an organism’s fitness. In balancing the cost of infection and the cost of immunity, selection should only favour individuals that allocate more energy to resistance and immune responses in populations regularly exposed to debilitating parasites. Here, we compare the ability of amphipods, Paracalliope novizealandiae, to (1) avoid becoming infected and (2) to respond to infection by encapsulating and melanizing parasites, between two natural populations exposed to different risk of parasitism. One population faces high levels of infection by the debilitating trematode parasite Maritrema novaezealandensis, whereas the other population is not parasitised by this trematode nor by any other parasite. Under controlled experimental conditions, with exposure to a standardized dose of parasites, amphipods from the parasite-free population acquired significantly more parasites than those from the population regularly experiencing infection. Furthermore, a lower frequency of amphipods from the parasite-free population succeeded at melanizing (and thus killing) parasites, and they melanized a lower percentage of parasites on average, than amphipods from the parasitised population. These differences persist when individual factors, such as amphipod sex or body length, are taken into account as potential confounding variables. These results support the existence of local adaptation against parasites: an amphipod population that never experiences trematode infections is less capable of resisting infection, both in terms of its first line of defence (avoiding infection) and a later line of defence (fighting parasites following infection), than a population regularly exposed to infection.     

Isotopic evidence for in-lake production of accumulating nitrate in Lake Superior.

A century-long increase in nitrate (NO3-) in the water column of Lake Superior is a classic example of recent nitrogen accumulation in ecosystems, but its cause and relationship to historical NO3- deposition is unknown. We used stable isotope ratios of oxygen and nitrogen in nitrate (delta18O-NO3 and delta15N-NO3) to examine its sources in this large lake, which represents 10% of the world's surficial liquid freshwater. The most parsimonious hypothesis to explain the rise in NO3- is that the lake is accruing NO3- deposited directly on the lake surface because it is too unproductive to completely assimilate all of it. Data for delta18O-NO3 in external sources and the water column, however, are inconsistent with this hypothesis. Instead, the isotopic evidence indicates strongly that the accumulating NO3- is almost entirely derived from nitrification occurring within the lake. While increases in atmospheric deposition of NO3- may have played a role in its buildup in the lake, other factors such as increases in NH4+ and dissolved organic nitrogen inputs from precipitation or rivers, increases in nitrogen fluxes from the sediments, and decreases in burial rates must also be considered as potential drivers of rising NO3-. The sustained accumulation of nitrogen in Lake Superior is thus more complex and incompletely understood than previously assumed.     

Ovariole number—a predictor of differential reproductive success among worker subfamilies in queenless honeybee (Apis mellifera L.) colonies

A honeybee queen normally mates with 10–20 drones, and reproductive conflicts may arise among a colony’s different worker patrilines, especially after a colony has lost its single queen and the workers commence egg laying. In this study, we employed microsatellite markers to study aspects of worker reproductive competition in two queenless Africanized honeybee colonies. First, we determined whether there was a bias among worker patrilines in their maternity of drones and, second, we asked whether this bias could be attributed to differences in the degree of ovary activation of workers. Third, we relate these behavioral and physiological factors to ontogenetic differences between workers with respect to ovariole number. Workers from each of three (colony A) and one (colony B) patrilineal genotypes represented less than 6% of the worker population, yet each produced at least 13% of the drones in a colony, and collectively they produced 73% of the drones. Workers representing these genotypes also had more developed follicles and a greater number of ovarioles per ovary. Across all workers, ovariole development and number were closely correlated. This suggests a strong effect of worker genotype on the development of the ovary already in the postembryonic stages and sets a precedent to adult fertility, so that “workers are not born equal”. We hypothesize a frequency-dependent or “rare patriline” advantage to queenless workers over the parentage of males and discuss the maintenance of genetic variance in the reproductive capacity of workers.Electronic supplementary material Supplementary material is available for this article at and is accessible for authorized users.     

Analysis of lead in soils adjacent to an interstate highway in Tampa,Florida

In order to assess the amount and distribution of lead pollution in soils adjacent to a major interstate highway serving the city of Tampa, Florida, a total of 224 samples were collected from 32 transects perpendicular to the roadway. The lead content was measured using atomic absorption spectrophotometry. The highest levels of lead were found at distances of 81, 243, and 729 cm from the road. The results show that there is a weak negative correlation between soil lead and the distance from the roadside, as well as with traffic density. The weakness of the relationship is a result of confounding variables such as turbulence and other microclimatic factors, downslope movement of soils overtime, and human action such as construction and highway landscaping. Nevertheless, over one-third of the samples collected in the study area contain more than 500 g g^–1 lead, levels considered to be hazardous by the United States Centers for Disease Control and the Environmental Protection Agency.     

Effects of worker genotypic diversity on honey bee colony development and behavior (Apis mellifera L.)

There have been numerous reports of genetic influences on division of labor in honey bee colonies, but the effects of worker genotypic diversity on colony behavior are unclear. We analyzed the effects of worker genotypic diversity on the phenotypes of honey bee colonies during a critical phase of colony development, the nest initiation phase. Five groups of colonies were studied (n = 5–11 per group); four groups had relatively low genotypic diversity compared to the fifth group. Colonies were derived from queens that were instrumentally inseminated with the semen of four different drones according to one of the following mating schemes: group A, 4 A-source drones; group B, 4 B-source drones; group C, 4 C-source drones; group D, 4 D-source drones; and group E, 1 drone of each of the A-D drone sources. There were significant differences between colonies in groups A-D for 8 out of 19 colony traits. Because the queens in all of these colonies were super sisters, the observed differences between groups were primarily a consequence of differences in worker genotypes. There were very few differences (2 out of 19 traits) between colonies with high worker genotypic diversity (group E) and those with low diversity (groups A-D combined). This is because colonies with greater diversity tended to have phenotypes that were average relative to colonies with low genotypic diversity. We hypothesize that the averaging effect of genotypic variability on colony phenotypes may have selective advantages, making colonies less likely to fail because of inappropriate colony responses to changing environmental conditions.     

Probabilistic prediction of exposures to arsenic contaminated residential soil

Probabilistic modelling using Monte Carlo simulation has been proposed as a more scientifically valid method of estimating soil contaminant exposures than conservative deterministic methods currently used by regulatory agencies. A retrospective application of probabilistic modelling to an exposure scenario involving arsenic-contaminated residential soil near the former ASARCO smelter near Tacoma, Washington is presented. The population of interest is children, aged 2–6 years, living within one-half mile (0.3 km) of the smelter site. Models that predict urinary arsenic levels based on unintentional soil ingestion and inhalation exposure pathways are used. Distributions of exposure variables are based on site-specific data and previous exposure studies. Simulated urinary arsenic levels are compared with data from two biomonitoring studies performed during the late 1980s. Arsenic distributions produced by simulation and biomonitoring are significantly different, and likely contributors to this difference are discussed. However the probabilistic model provides closer estimations of urinary arsenic levels than conservative deterministic models similar to those used by regulatory agencies, and provides useful information regarding parameter uncertainty. Soil ingestion rate was a driving variable in the probabilistic models. Further quantification of soil ingestion rates is warranted.     

Risk-taking by female ducks: intra- and interspecific tests of nest defense theory

We tested several predictions of nest defense theory by observing variation in flushing distance and probability of nest abandonment within and between six species of waterfowl. In these species, only the females incubate eggs and attend offspring. First, we examined whether flushing distance by females varied in relation to clutch size, stage of incubation, and time of season, after controlling for the number of visits made to nests by observers. Revisits by observers appeared to affect flushing distance by females for reasons unrelated to the relative value of the current clutch. We found that as incubation progressed, females allowed observers to approach more closely before flushing from the nest. In some species, females with larger clutches allowed closer approaches to nests before flushing which was also consistent with nest defense theory. In contrast, time of season (Julian date) did not relate to flushing distance for any species. When species were compared, we found that species with moderate to high yearly mortality and high reproductive output per breeding attempt (e.g., northern shoveler and blue-winged teal) were less likely to abandon nesting attempts and exhibited riskier behavior (remained at nests when approached closely by observers) than species that had lower yearly mortality (e.g., mallard). Our results show that flushing distance and patterns of nest abandonment by female ducks conform to several predictions of nest defense theory.Correspondence to: M.R.L. Forbes     

Effects of interactions among genotypically diverse nestmates on task specialization by foraging honey bees (Apis mellifera)

Summary Recent studies have shown that differences in patterns of task specialization among nestmate honeybee workers (Apis mellifera) can be explained, in part, as a consequence of genotypic variability. Here, we present evidence supporting the hypothesis that an individual's pattern of task specialization is affected not only by her own genotype, but, indirectly, by the genotypes of her nestmates. Workers from two strains of honey bees, one selected for high pollen hoarding, the other for low pollen hoarding, were observed in colonies of their respective parent strains and in colonies of the other strain. Worker genotype and host-colony type affected foraging activity. Workers from the high strain fostered in low-strain colonies returned with pollen on 75.6% of total foraging trips, while workers from the high strain fostered in high-strain colonies returned with pollen on 53.5% of total trips. Workers from the low strain fostered in low-strain colonies returned with pollen on 34.8% of total foraging trips while workers from the low strain fostered in high-strain colonies returned with pollen on 2.6% of total trips. Similar results were obtained in a second experiment. We suggest that workers influence the behavior of their nestmates indirectly through their effects on the shared colony environment. The asymmetry seen in the response of workers from these strains to the two types of colony environments also suggests that these genotypes exhibit different norms of reaction. Offprint requests to: N.W. Calderone     

Phylloplane algae of standing dead Spartina alterniflora

Phylloplane (leaf surface) algae on leaves from standing dead Spartina alterniflora Loisel in Sapelo Island marshes were enumerated by epifluorescence microscopy. The green alga Pseudendoclonium submarinum Wille dominated algal biovolume on both short-and tall-form plants during summer and winter. Intra-leaf and intra-plant patterns of algal biovolume and diversity indicated that desiccation stress may be an important selective factor. Observed epiphyte densities are 10- to 200-fold lower than values reported for communities on continuously submerged aquatic vegetation. Algal biovolume was less than 10% of that contained in the saprophytic (fungal and bacterial) community.     

An empirical analysis of economic strategies for controlling air pollution

In evaluating current environmental protection policy, economists often note that current regulations are more costly than necessary to meet environmental quality standards. While the a priori case is strong that current regulatory approaches are resulting in higher-than-necessary costs to attain environmental standards, there is relatively little empirical evidence to support this claim. The purpose of this paper is to supply some of the missing evidence by presenting the results of one study that assesses some of the potential savings associated with implementing economic, rather than command-and-control, regulatory approaches to abate one type of air pollution in one region of the country. Specifically, the paper examines the costs of meeting a prospective short-term standard for nitrogen dioxide under a range of alternative emissions control strategies for stationary sources of nitrogen oxide emissions in the Chicago Air Quality Control Region. The alternative strategies that are considered range from those that might result under current regulatory policy to those that economic policy approaches (such as emissions charges or marketable permits) are designed to implement. The analysis shows that the most efficient program of emissions controls may be more than an order of magnitude less costly than current regulatory strategies, and that economic approaches have additional advantages over more conventional regulatory approaches.