Micronucleus and different nuclear abnormalities in wild birds in the Cerrado,Brazil

Environmental Science and Pollution Research - Free-living birds play an important role as bioindicators in natural environments. In this study, we used the micronucleus test and nuclear...     

Evaluation of combustion by-products of MTBE as a component of reformulated gasoline

Methyl tertiary-butyl ether (MTBE) is a gasoline oxygenate that is widely used throughout the US and Europe as an octane-booster and as a means of reducing automotive carbon monoxide (CO) emissions. The combustion by-products of pure MTBE have been evaluated in previous laboratory studies, but little attention has been paid to the combustion by-products of MTBE as a component of gasoline. MTBE is often used in reformulated gasoline (RFG), which has chemical and physical characteristics distinct from conventional gasoline. The formation of MTBE by-products in RFG is not well-understood, especially under "worst-case" vehicle emission scenarios such as fuel-rich operations, cold-starts or malfunctioning emission control systems, conditions which have not been studied extensively. Engine-out automotive dynamometer studies have compared RFG with MTBE to non-oxygenated RFG. Their findings suggest that adding MTBE to reformulated gasoline does not impact the high temperature flame chemistry in cylinder combustion processes. Comparison of tailpipe and exhaust emission studies indicate that reactions in the catalytic converter are quite effective in destroying most hydrocarbon MTBE by-product species. Since important reaction by-products are formed in the post-flame region, understanding changes in this region will contribute to the understanding of fuel-related changes in emissions.     

Report: Combustion Byproducts and Their Health Effects: Summary of the 10th International Congress

The 10th International Congress on Combustion Byproducts and their Health Effects was held in Ischia, Italy, from June 17-20, 2007. It is sponsored by the US NIEHS, NSF, Coalition for Responsible Waste Incineration (CRWI), and Electric Power Research Institute (EPRI). The congress focused on: the origin, characterization, and health impacts of combustion-generated fine and ultrafine particles; emissions of mercury and dioxins, and the development/application of novel analytical/diagnostic tools. The consensus of the discussion was that particle-associated organics, metals, and persistent free radicals (PFRs) produced by combustion sources are the likely source of the observed health impacts of airborne PM rather than simple physical irritation of the particles. Ultrafine particle-induced oxidative stress is a likely progenitor of the observed health impacts, but important biological and chemical details and possible catalytic cycles remain unresolved. Other key conclusions were: (1) In urban settings, 70% of airborne fine particles are a result of combustion emissions and 50% are due to primary emissions from combustion sources, (2) In addition to soot, combustion produces one, possibly two, classes of nanoparticles with mean diameters of?~10?nm and?~1?nm. (3) The most common metrics used to describe particle toxicity, viz. surface area, sulfate concentration, total carbon, and organic carbon, cannot fully explain observed health impacts, (4) Metals contained in combustion-generated ultrafine and fine particles mediate formation of toxic air pollutants such as PCDD/F and PFRs. (5) The combination of metal-containing nanoparticles, organic carbon compounds, and PFRs can lead to a cycle generating oxidative stress in exposed organisms.     

Active and effective nest defence by males in a social apoid wasp

Altruism in the social Hymenoptera is generally considered to be a feature of females rather than males. A popular explanation for this is that in the solitary ancestors of today's social species, males provided little brood care. Males might therefore lack the preadaptations necessary to evolve altruism in social contexts. While anecdotal observations of male contributions to colony life have been reported, there are few reports of male participation in nest defence. In apoid wasps, there have been several reports of male nest-guarding behaviour in solitary species, potentially setting the evolutionary stage for similar behaviours in social lineages. Here, we present evidence of active and effective nest defence in males of the social apoid wasp Microstigmus nigrophthalmus. Males were observed chasing intruders away from the nest, and the presence of males had a significant effect on nest survival when females were removed. Males potentially obtained direct benefits through defence, so that defence may not represent male altruism. However, our results do show that males can perform acts that benefit their colony.     

Coelomocyte biomarkers in the earthworm Eisenia fetida exposed to 2,4,6-trinitrotoluene (TNT)

Contamination by 2,4,6-trinitrotoluene (TNT) is a global environmental problem at sites of former explosive production, handling, or storage, and could have deleterious consequences for human and ecological health. We investigated its sublethal effects to Eisenia fetida, using two nonspecific biomarkers. In coelomocytes of earthworms exposed 24, 48, or 72 h, we evaluated DNA damage (comet assay) and neutral red retention time (NRRT), using the filter paper contact test. Both percentage of damage (D%) and calculated damage index showed significant DNA damage at almost all concentrations, at all time points assayed. Along exposure time, two different patterns were observed. At the lower TNT concentrations (0.25?C0.5 ??g/cm²) an increased DNA migration at 48 h, with a decrease close to initial levels after 72 h exposure, was observed. This decrease could be attributed to activation of the DNA repair system. At higher concentrations (1.0?C2.0 ??g/cm²), the high DNA damage observed remained constant during the 72 h exposure, suggesting that the rate of DNA repair was not enough to compensate such damage. Analysis of NRRT results showed a significant interaction between time and treatment. After 48 h, a significant decrease was observed at 4.0 ??g/cm². After 72 h, NRRT presented a concentration-dependent decrease, significantly different with respect to control at 0.5, 1.0, 2.0, and 4.0 ??g/cm². The two assayed methods, performed on the same sample, showed clear responses to sublethal TNT exposure in E. fetida, providing sensitive unspecific biomarkers of cell injury and DNA damage.     

Hydrophobic Organic Matter Promotes Coxsackievirus B5 Stabilization and Protection from Heat

Food and Environmental Virology - In urban rivers, many of which are used for drinking water production, viruses encounter a range of particulate, colloidal, and dissolved organic and inorganic...     

Recycling of extruded polystyrene wastes by dissolution and supercritical CO2 technology

Polystyrene (PS) is currently used as packaging, insulating and storing material in various industrial or domestic fields. As a result, a large quantity of PS wastes is produced. Plastic wastes are not usually biodegradable, so it is necessary to suggest a technology to recycle them. Landfills and incineration are reasonably cheap methods but are not environmentally acceptable, therefore, alternative methods for polymer recycling are required. The general purpose of PS foam recycling is to recover a more compact polymeric material without degradation. Dissolution with terpenic solvents is presented here as an efficient and cheap alternative that is developed at room temperature; among the oils studied, limonene was selected because of its intermediate solubility and its abundance. The solvent removal is possible thanks to supercritical technology that provides a high solubility in limonene and almost a complete PS insolubility at moderated pressures (77?bar) and low temperatures (30?°C). Thus, based on the results of thermogravimetric and chromatographic analysis, we propose that.supercritical antisolvent precipitation is an ideal technique for carrying out the separation of PS and limonene, providing a recycled polymer with a reduced volume, almost completely free of solvent and without degradation of the polymeric chains.     

Estimating digestion time in gelatinous predators: a methodological comparison with the scyphomedusa Aurelia aurita

In order to quantify the trophic impact of gelatinous predators, digestion time estimates are commonly applied to counts of prey in the guts. Three primary approaches are used, the Manual-feeding, Natural-feeding and Steady-state methods; these differ in methodology and their underlying assumptions. The criteria used to define the end-point of digestion, and the resolution at which digestion progress is observed, also vary across studies. To understand the impact of such differences, we estimate digestion times of the scyphomedusa Aurelia aurita fed adult females of the copepod Acartia tonsa using these various approaches. We find ~fourfold differences which can be attributed to bias towards the slowest rates of digestion by some end-point criteria, and overestimation from low observation resolution. Artificial manipulation and the degree to which swimming and feeding behaviour are natural may also influence estimates. We provide recommendations for those quantifying digestion times of Aurelia aurita medusae and gelatinous predators.     

Challenges for a sustainable resource use: Uncertainty,trade, and climate policies

We integrate new challenges to thinking about resource markets and sustainable resource use policies in a general framework. The challenges, emerging from six papers that JEEM publishes in a special issue, are (i) demand uncertainty and stockpiling, (ii) international trade and resource dependence, (iii) deforestation, and (iv) intertemporal effects of climate change policies (“Green Paradox”). We discuss new insights and results on these issues by fitting them into the Hotelling model of non-renewable resource depletion.