The capacity of duckweed to treat wastewater: ecological considerations for a sound design

Duckweed species are promising macrophytes for use in sustainable wastewater treatment due to their rapid growth, ease of harvest, and feed potential as a protein source. This paper reviews growth rates of different duckweed species on wastewater and ammonia toxicity to duckweed and summarizes insights into the mechanism of organic matter and nutrient removal. Results were gained from laboratory experiments in small, shallow, duckweed-covered semicontinuous batch systems. Growth rates on different types of wastewater vary considerably among different species. Ammonia is toxic for duckweed in both the ionized and un-ionized forms. Duckweed, however, can be used to treat wastewater containing very high total ammonia concentrations as long as certain pH levels are not exceeded. The degradation of organic material is enhanced by duckweed through both additional oxygen supply and additional surface for bacterial growth. The duckweed mat with attached bacteria and algae is, independent of the loading rates, responsible for three-quarters of the total nitrogen (N) and phosphorus (P) loss in very shallow systems. Based on our results we suggest that full-scale pilot plants with duckweed should be shallower than the range encountered in the literature. A harvesting schedule that allows doubling times of 2 to 3.5 d, maintenance of a full coverage, and plug flow conditions are recommended.     

HYDROLOGIC STAGE PERIODICITY OF THE MISSISSIPPI RIVER BEFORE AND AFTER SYSTEMATIC CHANNEL MODIFICATIONS1

ABSTRACT: Periodic flood disturbance is a well known controlling factor of in channel and floodplain ecosystem function. However, channel manipulations during the last century have potentially altered hydrologic fluctuations, and thus ecosystem function. We examined temporal river stage hydrology, through autocorrelation analysis, at seven gauges along the Mississippi River to quantify flow periodicity and effects of systematic channel modifications on flow periodicity. Intraannual variation follows a strong one‐year cycle of six months higher flow and six months lower flow for the entire Mississippi River drainage, with precipitation as a driving force. Interannual hydrologic variation differs between the upper and lower river segments. A clear quasi‐biennial oscillation pattern was evident throughout the lower river section. The effect of channel alterations was a decreased magnitude of differences between lower and higher flows. The upper section, however, suggests a 12‐to 14‐year periodicity prior to alterations and a decreased duration of lower flow years following systematic modifications. Interannual variograms clearly depict very different temporal hydrology between the upper Mississippi River and the lower Mississippi River, suggesting the simple transfer of knowledge from one segment to the other oversimplifies the complexity of a large river system.     

Thermal manikin measurements--exact or not?

According to the European prestandard ENV 342:1998, the thermal insulation of cold-protective clothing is measured with a thermal manikin. Systematic studies on the reproducibility of the values, measured with different types of clothing on the commonly used standing and walking manikins, have not been reported in the literature. Over 300 measurements were done in 8 different European laboratories. The reproducibility of the thermal insulation test results was good. The coefficient of variation was lower than 8%. The measured clothing should fit the manikin precisely, because poorly fitting clothing gave an error in the results. The correlation between parallel and serial insulation values was excellent and parallel values were about 20% lower than serial ones. The influence of ambient conditions was critical only in the case of air velocity. The reproducibility of thermal insulation test results in a single laboratory was good, and the variation was lower than 3%.     

Lack of social entrainment of free-running circadian activity rhythms in the Australian sugar glider (Petaurus breviceps: Marsupialia)

Summary Social influence on circadian activity rhythms was investigated in the nocturnal Australian marsupial Petaurus breviceps. The activity of two and two was recorded electroacoustically and observed by an IR television camera in LD 12:12 (10¹:10^-1lx) and in LL (10^-1lx) when housed isolated and in pairs (+). In LD-entrained animals the average duration of locomotion, orientation movements, grooming and total activity is influenced by social housing, and individually different activity patterns are harmonized. In constant illumination conditions, however, the members of a pair are not mutually synchronized and free-run with different circadian periodlengths. Therefore social influence on the LD-entrained activity rhythm in Petaurus should be interpreted as social masking rather than direct influence on the circadian system.     

On the significance of adult play: what does social play tell us about adult horse welfare?

Play remains a mystery and adult play even more so. More typical of young stages in healthy individuals, it occurs rarely at adult stages but then more often in captive/domestic animals, which can imply spatial, social and/or feeding deprivations or restrictions that are challenging to welfare, than in animals living in natural conditions. Here, we tested the hypothesis that adult play may reflect altered welfare states and chronic stress in horses, in which, as in several species, play rarely occurs at adult stages in natural conditions. We observed the behaviour (in particular, social play) of riding school horses during occasional outings in a paddock and measured several stress indicators when these horses were in their individual home boxes. Our results revealed that (1) the number of horses and rates of adult play appeared very high compared to field report data and (2) most stress indicators measured differed between ‘players’ and ‘non-players’, revealing that most ‘playful’ animals were suffering from more chronic stress than ‘non-playful’ horses. Frequency of play behaviour correlated with a score of chronic stress. This first discovery of a relationship between adult play and altered welfare opens new lines of research that certainly deserves comparative studies in a variety of species.     

Comparative evaluation of four suspended particulate matter (SPM) sampling devices and their use for monitoring SPM quality

Environmental Science and Pollution Research - Representative sampling of suspended particulate matter is fundamental for assessing river sediment quality, including the distribution and...     

Considerations of temperature in the context of the persistence classification in the EU

Simulation degradation studies for industrial chemicals, biocidal products and plant protection products are required in the EU to estimate half-lives in soil, water and sediment for the comparison to persistence criteria for hazard (P/vP) assessment, and for use in exposure assessments. There is a discrepancy between European regulatory approaches regarding the temperature at which degradation half-lives should be (1) measured in simulation degradation testing of environmental compartments, and (2) compared to the P/vP criteria. In this paper, an opinion is provided on the options for the experimental temperature and extrapolation to other conditions. A review of the historical development of persistence criteria did not give conclusive evidence of the temperature at which the half-lives that underpin the P-criteria were measured, but room temperature is likely. Half-lives measured at 20 °C are in line with the intentions of some international agreements, but in the EU there is a continued political debate regarding the relevant temperature for comparison with persistence criteria. Measuring degradation at 20 °C has the advantage that metabolites/transformation products can be identified with greater accuracy, and that kinetic fits to determine half-lives for parent compounds and metabolites carry less uncertainty. Extrapolation of half-lives to lower temperatures is possible for assessing environmental exposure, but the uncertainty of the persistence classification is smaller when measured half-lives are used for direct comparison with P/vP criteria, without extrapolation. Model simulations demonstrate the pattern of concentrations that can be expected for realistic worst case climate scenarios in the EU based on the half-life of 120 days in soil at 20 °C and of 40 days in water at 20 °C, and their temporal and spatial variability.     

Influence of the nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) on ammonia-oxidizing bacteria and archaea in rhizosphere and bulk soil

In agricultural plant production nitrification inhibitors like 3,4-dimethylpyrazole phosphate (DMPP) are used to retard the microbial nitrification process of fertilized ammonium to enhance the nitrogen supply for cultivated crops and to reduce nitrogen losses from the production system. Besides the well-known ammonia-oxidizing bacteria (AOB) it is known for a few years that also ammonia-oxidizing archaea (AOA) are able to perform the first step in nitrification, hence being also a target for a nitrification inhibitor. However, so far no information are available concerning the effectiveness of DMPP and its extent towards AOB and AOA, neither in bulk soil nor in the root-rhizosphere complex. We investigated in a field experiment performed according to agricultural practice the effect of DMPP on the abundance of AOB and AOA two, four and eight weeks after fertilization. We observed impaired abundances of AOB but not of AOA in both soil compartments that were still visible eight weeks after application, possibly indicating a reduced effectiveness of the nitrification inhibitor in our study.     

High levels of natural radioactivity in biota from deep-sea hydrothermal vents: a preliminary communication

Hydrothermal deep-sea vent fauna is naturally exposed to a peculiar environment enriched in potentially toxic species such as sulphides, heavy metals and natural radionuclides. It is now well established that some of the organisms present in such an environment accumulate metals during their lifespan. Though only few radionuclide measurements are available, it seems likely that hydrothermal vent communities are exposed to high natural radiation doses. Various archived biological samples collected on the East Pacific Rise and the Mid-Atlantic Ridge in 1996, 2001 and 2002 were analysed by ICP-MS in order to determine their uranium contents (²³⁸U, ²³⁵U and ²³⁴U). In addition ²¹⁰Po–Pb were determined in 2 samples collected in 2002. Vent organisms are characterized by high U, and Po–Pb levels compared to what is generally encountered in organisms from outside hydrothermal vent ecosystems. Though the number of data is low, the results reveal various trends in relation to the site, the location within the mixing zone and/or the organisms' trophic regime.     

Air parcel model simulations of a convective cloud: Bacteria acting as immersion ice nuclei

The effects of bacteria acting as immersion ice nuclei were investigated in numerical sensitivity studies and compared to the efforts of other ice nuclei such as mineral dust and soot particles. An adiabatic air parcel model was employed simulating convective situations with different initial aerosol particle distributions. The maximum fractions of active ice nuclei were based on field measurements of the proportioning of atmospheric aerosol particle types in continental and marine air masses. Recent field measurements of bacteria concentrations in cloud water and in snow samples were used. From the concentrations in bulk samples the concentration in mean sized cloud droplets was estimated. Immersion freezing was described based on laboratory measurements to constrain the freezing fraction versus temperature. The results indicated that the effects of diminutive amounts of bacteria on ice formation in convective clouds, while being significantly less than the effects of mineral dust particles, might be comparable to the expected effects of soot particles acting as ice nuclei. It can be predicted that bacterial ice nuclei would have to be enriched by at least 10⁴ times reported concentrations in cloud water in order to equate to the impact of mineral dust ice nuclei present in 20–25% of all cloud droplets.