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21.
A method was developed for the determination of benzene in urine. The sample was pumped through the donor channel of a membrane extraction unit with a micro-porous membrane, separating the donor channel from an identical acceptor channel purged with nitrogen. The analyte reached the acceptor channel by diffusion through the membrane and was then swept by the carrier to a solid sorbent tube, where it was trapped. The analyte was subsequently thermally desorbed and analyzed by gas chromatography (GC) with mass selective detection (MS). After optimization, the recovery was close to quantitative, or 95%. Purging the membrane unit with pure water in between the samples eliminated any memory effects. The linearity was good in the concentration range examined (20-4000 ng l(-1)), with a correlation coefficient of 0.9996. The repeatability at 50 ng l(-1) and 400 ng l(-1) was 1.4% and 1.2%, respectively. The limit of detection was 12 ng l(-1) and the limit of quantification 35 ng l(-1). This enables assessment of benzene exposures of occupationally exposed subjects, of smokers and the majority of the general population. The developed method can be easily automated. 相似文献
22.
B. Beck-Friis M. Pell U. Sonesson H. Jönsson H. Kirchmann 《Environmental monitoring and assessment》2000,62(3):317-331
Composting can be a source of N2O andCH4 production. In this investigation, differentcompost heaps of organic household waste weremonitored with the focus on potential formation ofCH4 and N2O in the heaps and emission ofthese gases from the heaps. The studied compost heapshad different compost ages, turning intervals andcompost sizes. The analysed compost gases containedbetween 1–3421 L of N2O-N L-1 and 0–470 mL of CH4 L-1. The emission rates ofN2O and CH4 from the compost heaps werebetween 1–1464 mg N2O m-2 day-1 and0–119 000 mg CH4 m-2 day-1. These verylarge differences in compost gas composition andemission indicate the importance of compostmanagement. The results also give an understanding ofwhere in the composting process an increasing emissionof N2O and CH4 can occur. 相似文献
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Specific PCR amplification and probe hybridization techniques were applied to examine the compositions of airborne fungi in samples from three different environments. The results from microscopic and CFU counting were compared to those of the molecular-based detections. The detection sensitivity for PCR amplifications was 9 to 73 spores and 1.3 to 19.3 CFUs per PCR reaction. The hybridization detection limit was 2 to 4 spores and 0.2 to 1.2 CFU. The hybridization method was more sensitive than PCR amplification and showed less variation among samples. Using specific PCR primers and probes we identified the presence of several fungal groups and species in the air samples. Specific detections through probe hybridization to PCR products amplified with universal or group-specific fungal primers have promising applications in the examination of air samples for environmental monitoring. 相似文献
25.
The importance of selenium as an essential trace element has progressively emerged during the last years due to the analysis of selenium deficiency diseases and to the identification and characterization of a number of selenoenzymes. Selenium is incorporated in the catalytic site of the enzymes as an integral selenocysteine residue. The pathway of selenocysteine biosynthesis and incorporation has been elucidated recently for Escherichia coli. This article presents an overview on these subjects and describes the mechanisms which confer selenocysteine specificity in the framework of protein biosynthesis. In addition, some considerations concerning the phylogeny of selenocysteine incorporation are presented and a model for the evolution of the selenocysteine pathway is proposed. 相似文献
26.
E. Lamla F. Boschke G. Traving G. Pfotzer H. Wolter W. Walcher H. D. Dahmen H. J. Queisser W. W. Klingbeil K. Beyermann O. Müller R. R. Ernst D. Reinen H. Muth W. Becker G. Wunsch G. I. Distler L. Jaenicke H. G. v. Schnering H. Werner F. Reiff H. Schimassek W. Höll H. K. Vasil W. F. Angermeier W. Ruppel J. C. Eccles G. Spiteller H. Oettel W. Vogell P. Brosche K. J. Hsü H. -U. Schmincke 《Die Naturwissenschaften》1975,62(3):143-143
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