Intracellular biosynthesis of Au and Ag nanoparticles using ethanolic extract of Brassica oleracea L. and studies on their physicochemical and biological properties

In this present study, we reported broccoli(Brassica oleracea L.) as a potential candidate for the synthesis of gold and silver nanoparticles(NPs) in green chemistry method. The synthesized metal nanoparticles are evaluated their antimicrobial efficacy against different human pathogenic organisms. The physico-chemical properties of gold nanoparticles were analyzed using different analytical techniques such as a UV–Vis spectrophotometer, Field Emission Scanning Electron Microscopy, energy dispersive X-ray spectroscopy, X-ray diffraction and a Fourier Transform Infrared spectrophotometer. In addition, gold and silver NP antimicrobial efficacy was checked by disc diffusion assay. UV–Vis color intensity of the nanoparticles was shown at 540 and 450 nm for gold and silver nanoparticles respectively. Higher magnification of the Field Emission Scanning Electron Microscopy image shows the variable morphology of the gold nanoparticles such as spherical, rod and triangular shapes and silver nanoparticles were seen in spherical shapes. The average spherical size of the particles was observed in 24–38 nm for gold and 30–45 nm for silver NPs. X-ray diffraction pattern confirmed the presence of gold nanoparticles and silver nanoparticles which were crystalline in nature. Additionally,the functional metabolites were identified by the Fourier Transform Infrared spectroscopy. IR spectra revealed phenols, alcohols, aldehydes(sugar moieties), vitamins and proteins are present in the broccoli extract which are accountable to synthesize the nanoparticles. The synthesized gold and silver NPs inhibited the growth of the tested bacterial and fungal pathogens at the concentration of 50 μg/m L respectively. In addition, broccoli mediated gold and silver nanoparticles have shown potent antimicrobial activity against human pathogens.     

环境与发展

本文论述了环境与发展的关系,并简要介绍了可持续发展的基本理论,在此基础上阐述了在中国如何实施可持续发展战略。     

《斯德哥尔摩公约》的实施进展及相关国际活动研究

本文总结了《斯德哥尔摩公约》签署2年多来,国际社会和世界主要国家为公约的最后生效实施所做努力及公约的执行进展。分析了各国针对履行POPs公约、控制POPs污染影响所采取的国家行动计划及其主要特点。这些相关信息可为我国制定履行POPs公约的国家实施计划(NIP)及相关的策略选择提供依据和参考。     

室内环境中化学污染物质测定法研究

室内环境中化学污染物质的测定是研究和解决室内环境问题的基础。本文以甲醛和挥发性有机化合物两种室内环境中的主要化学污染物质为例,介绍了目前世界各国采用的各种测定法及其动向,并探讨了测定时期、测定时间、采样点布置等有关问题,提出了一些看法供参考。     

城市生活垃圾处理现状及发展趋势

针对我国目前城市生活垃圾的现状和特点,探讨了城市垃圾产生量随年度变化的规律,垃圾产生量与人口和GDP的关系,分析了我国主要城市的垃圾组分。简要介绍了处理城市生活垃圾的几种方法和处理技术的发展趋势。     

地震火灾事例调查

本文介绍了世界上典型的几个地震火灾的例子,汇总了中外地震史上曾发生过的地震火灾,并且较详细地介绍了产生地震火灾的原因。     

中国环境监测总站未来十年发展构想

阐述了总站未来十年发展的指导思想、目标和原则,明确了总站未来十年发展的重点业务领域,介绍了实现构想的保证措施和重点建设项目。     

参数化绘图系统的开发与应用

探讨了参数化绘图系统开发的基本算法、功能和要求及开发步骤,简单介绍了国内外参数化CAD的应用情况。     

Synthesis, separation and identification of dinitropyrene isomers

Dinitropyrenes (DNP) were prepared by nitration of pyrene, and the product was characterized by MS and elemental analysis. Three isomers of DNP were separated by HPLC and identified by 1H-NMR. The eating sequence on the normal phase column is 1, 3-DNP, 1, 6-DNP and 1, 8-DNP, whereas is 1, 6-DNP, 1. 8-DNP and 1. 3-DNP on the reversed phase column. The separation of three DNP isomers under different chromatographic conditions was also discussed.