Behavioral and electrophysiological responses of the emerald ash borer, Agrilus planipennis, to induced volatiles of Manchurian ash, Fraxinus mandshurica

Summary. We investigated the volatile emissions of Manchurian ash seedlings, Fraxinus mandshurica, in response to feeding by the emerald ash borer, Agrilus planipennis, and to exogenous application of methyl jasmonate (MeJA). Feeding damage by adult A. planipennis and MeJA treatment increased volatile emissions compared to unexposed controls. Although the same compounds were emitted from plants damaged by beetles and treated with MeJA, quantitative differences were found in the amounts of emissions for individual compounds. Adult virgin female A. planipennis were similarly attracted to volatiles from plants damaged by beetles and those treated with MeJA in olfactometer bioassays; males did not respond significantly to the same volatiles. Coupled gas chromatographic-electroantennogram detection (GC-EAD) revealed at least 16 antennally-active compounds from F. mandshurica, including: hexanal, (E)-2-hexenal, (Z)-3-hexen-1-ol, 3-methyl-butylaldoxime, 2-methyl-butylaldoxime, (Z)-3-hexen-1-yl acetate, hexyl acetate, (E)-β-ocimene, linalool, 4,8-dimethyl-1,3,7-nonatriene, and E,E-α-farnesene. Electroantennogram (EAG) dose–response curves using synthetic compounds revealed that females had a stronger EAG response to linalool than males; and male responses were greater to: hexanal, (E)-2-hexenal, (Z)-3-hexen-1-ol, 3-methyl-butylaldoxime, 2-methyl-butylaldoxime, and hexyl acetate. These results suggest that females may use induced volatiles in long-range host finding, while their role for males is unclear. If attraction of females to these volatiles in an olfactometer is upheld by field experiments, host plant volatiles may find practical application in detection and monitoring of A. planipennis populations.     

Ecological risk assessment of agricultural pesticides in the highly productive Ndop flood plain in Cameroon using the PRIMET model

Flood plains can make a significant contribution to food security due to their rich agricultural and aquaculture potentials but the misuse of pesticides in these ecosystems may seriously threaten their sustainability. We have assessed the risk of commonly used pesticides by rice and vegetable farmers on two major streams in the Ndop flood plain in Cameroon using the PRIMET model. Thirty pesticide formulations were identified containing 17 active ingredients belonging to fungicides (7), herbicides (3), and insecticides (7). Out of these, five posed acute and/or chronic risks to the streams. Chlorpyriphos-ethyl (ETR = 655), chlorothalonil (ETR = 250), and cypermethrin (ETR = 190) posed a definite acute risk to both streams. These pesticides also posed possible chronic risks with cypermethrin also posing a definite chronic risk (ETR = 1658) to fish in one of the streams. Mancozeb (ETR = 2.2) and λ-cyhalothrin (ETR = 2.8) posed a possible acute risk to both streams. It was interesting to note that most pesticides, including some of those that posed a risk, were applied at lower doses than their respective recommended doses. Differences in ETRs were also observed in the two streams with higher values occurring in the stream having a lower concentration of suspended solids. The higher velocity of the Ngwala-Mesaw stream did not affect the acute risk posed by pesticides. Pesticide toxicity was an important determinant in the risk posed and we recommend that appropriate mitigation measures be put in place to reduce the risk of these pesticides such as the implementation of an appropriate buffer zone between the edge of field and water body.

     

A Comparison of Three Methods to Collect Submerged Aquatic Vegetation in a Shallow Lake

Two boat-based and one in-water sampling method have been used to collect submerged aquatic vegetation (SAV) as part of a long-term monitoring program in Lake Okeechobee, Florida, USA. The boat-based methods consisted of collecting SAV with a ponar dredge, used only to collect Chara, and an oyster tongs-like rake apparatus, used to collect all SAV. The in-water method involved use of a 0.5 m² PVC quadrat frame deployed by a diver. During summer 2002, SAV biomass samples were collected using all three methods at various sites in the lake to compare between-methods sampling precision. Sites used for these comparisons were selected based on plant type, plant density and sediment type. Statistical comparisons indicated that there were significant (p ≤ 0.05) biomass differences in 8 of 15 possible pairwise comparisons between sampling method biomass means. In four of the eight comparisons, significantly higher biomass mean values were obtained using the quadrat frame. In three of the remaining four comparisons, significantly higher biomass mean values were obtained with the rake apparatus. For the fourth comparison, a significantly higher biomass mean value was obtained with the ponar dredge. Three of the four relationships between SAV biomass collected by the rake and the quadrat and the rake and the quadrat/ponar dredge were statistically significant, linear and explained between 67 and 78% of the biomass variability. There were no significant differences between regression coefficients or elevations for these relationships, therefore these regressions estimated the same population regression. The population regression coefficient was 0.95, suggesting that the quadrat and ponar over-sampled relative to the rake, but the amount of this over-sampling was very small. Since there was no consistent pattern in the sampling method which yielded the significantly different biomass values and there were no significant differences in sampling precision across a range of plant species, plant densities and two sediment types, the boat-based rake method appears to be a suitable replacement for the previously used ponar dredge and quadrat methods, when in-lake measurements are not practical.     

A diffusive sampling device for the determination of formaldehyde in air using N-methyl-4-hydrazino-7-nitrobenzofurazan (MNBDH) as reagent

A new method utilizing the diffusive sampling of formaldehyde in air has been developed. Formaldehyde is sampled with the use of a glass fiber filter impregnated with N-methyl-4-hydrazino-7-nitrobenzofurazan (MNBDH) and phosphoric acid. The formaldehyde hydrazone formed is desorbed from the filter with acetonitrile and determined by high-performance liquid chromatography (HPLC) with UV/visible detection at 474 nm. The sampling rate was determined to be 24.7 mL min-1 with a relative standard deviation of 7% for 48 experiments. The measured sampling rates were not dependent on the formaldehyde concentration (0.1-1.0 mg m-3), sampling time (15-482 min) or relative humidity (20-85%). The detection limit was 70 micrograms m-3 for a 15 min sampling period and 2 micrograms m-3 for an 8 h sampling period.     

Environmental mainstreaming and policy coherence: essential policy tools to link international agreements with national development—a case study of the Caribbean region

The 2030 agenda for sustainable development adopted in September 2015 advocates for a balanced integration of the economic, social and environmental dimensions of sustainable development. Existing multilateral environmental agreements (MEAs) can provide guidance for policy-makers to promote inclusive and sustainable economic growth, within safe ecological limits. This paper presents a practical approach to mainstream international obligations of MEAs with national development priorities and strategies. It identifies pathways of policy coherence from national strategies to specific instruments and indicators to advance implementation of MEAs and help counties to fulfill agreed obligations. The approach is grounded in the driving forces–pressure–state–impact–response (DPSIR) framework, and it was applied in two national and two regional case studies in the Caribbean. The outcomes from the case studies show that mainstreaming rate of MEAs is low, and linkages across multiple MEAs are rarely considered. The DPSIR framework was useful for addressing national development priorities and those of MEAs in tandem, through adopting a cross-sectoral, multistakeholder perspective. The research shows that addressing environmental degradation and improving MEAs’ implementation requires that international and regional agencies identify linkages among MEAs to assist in creating policy coherence to ensure their integration into national strategies by connecting with policies and strategies in tandem implementation of MEAs for national policy-makers to work with.