Inhibition of pancreatic carboxypeptidase A: A possible mechanism of interaction between penicillic acid and ochratoxin A

Abstract

Penicillic acid and ochratoxin A are environmentally important toxic fungal metabolites (mycotoxins) that are synergistic in combination. The effects of penicillic acid on the pancreatic enzyme, :arboxypeptidase A were investigated in vitro and in vivo. A broad range of inhibition in vitro of the enzyme by PA was demonstrated with a half‐maximal inhibitory concentration equal to 1.1 x 10^‐4M PA. Inhibition of carboxypeptidase A was time and temperature dependent, and resulted in decreased conversion of parent ochratoxin A to the non‐toxic metabolite, ochratoxin alpha. Studies in vivo demonstrated a penicillic acid‐dependent inhibition of pancreatic carboxypeptidase A activity in the mouse and the chicken following multiple oral exposure. It is postulated that the mode of toxic interaction of the two mycotoxins may be due, in part, to impaired detoxification of ochratoxin A through peni‐cillic acid depletion of carboxypeptidase A activity.     

Teleworker knowledge sharing and the role of altered relational and technological interactions

Given the growing importance and complexities of telework and the challenges associated with knowledge sharing, in this study we investigate teleworkers and their propensity to share knowledge. We do so by investigating if the relational qualities of teleworkers in the form of trust, interpersonal bond, and commitment, act to impact teleworker knowledge sharing. We also investigate how telework's altered spatial and technical interactions shape knowledge sharing, by testing the contingent role of technology support, face-to-face interactions, and electronic tool use. Results using matched data from 226 teleworkers support the role of teleworker trust, interpersonal bond, and commitment in predicting knowledge sharing. Moreover, the impact of trust on knowledge sharing is found to be moderated by technology support, face-to-face interactions, and use of electronic tools, whereas the impact of commitment is contingent upon the use of electronic tools. Copyright © 2009 John Wiley & Sons, Ltd.     

Characterization of pollutants in Florida street sweepings for management and reuse

Disposal and beneficial-use options for street sweeping residuals collected as part of routine roadway maintenance activities in Florida, USA, were assessed by characterizing approximately 200 samples collected from 20 municipalities. Total concentrations (mg/kg or μg/kg) and leachable concentrations (mg/L or μg/L) of 11 metals and a number of organic pollutant groups (volatile organics, semi-volatile organics, pesticides, herbicides, carbamates) in the samples were measured. The synthetic precipitation leaching procedure (SPLP) was performed to evaluate the leachability of the pollutants. From the total metal analysis, several metals (e.g., arsenic, barium, chromium, copper, nickel, lead, and zinc) were commonly found above their detection limits. Zinc was found to have the highest mean concentration of all metals measured (46.7 mg/kg), followed by copper (10.7 mg/kg) and barium (10.5 mg/kg). The metal with the smallest mean concentration was arsenic (0.48 mg/kg). A small fraction of the total arsenic, barium, lead, and zinc leached in some samples using the SPLP; leached concentrations were relatively low. A few organic compounds (e.g., 4,4′-DDT, endrin, and endosulfan II) were detected in a limited number of samples. When the total and leaching results were compared to risk-based Florida soil cleanup target levels and groundwater cleanup target levels, the street sweepings were not found to pose a significant human-health risk via direct exposure or groundwater contamination.     

Socio-cognitive constraints and opportunities for sustainable intensification in South Asia: insights from fuzzy cognitive mapping in coastal Bangladesh

Environment, Development and Sustainability - Appreciating and dealing with the plurality of farmers’ perceptions and their contextual knowledge and perspectives of the functioning and...     

Idealized tracer transport models with time-varying transport: applications to ocean boundary currents

One-dimensional advection–diffusion and advection–diffusion–dilution (or “leaky-pipe”) models have been widely used to interpret a variety of geophysical phenomena. For example, in the ocean these tools have been used to interpret the penetration and spreading of tracers such as Chlorofluorocarbons(CFCs) along the Deep Western boundary current (DWBC). Usually, the transport coefficients of such models are taken to be constant in time, thus assuming the transport to be in steady state. Here, we relax this assumption and calculate tracer-signal variability in two simple 1D models for the boundary current having low-amplitude time-varying coefficients. Given a background tracer gradient due, for example, to a steady-state source in a boundary region, the resulting tracer field exhibits fluctuations due to the transport acting on the gradients. We compare the transport-induced tracer fluctuations to propagated fluctuations occurring in steady-state models with a periodic source in the boundary region. Using coefficients fitted to DWBC tracer observations, we find that in the North Atlantic propagated tracer fluctuations are larger, while in the sub-tropics transport-induced fluctuations dominate. This contrasts a common view that subtropical and tropical DWBC fluctuations in tracers such as CFCs, temperature and salinity anomalies are propagated signals from the northern formation region. However, the predicted transport-induced fluctuations in these models are still smaller than the observed fluctuations.     

Attenuation of hydrogen sulfide at construction and demolition debris landfills using alternative cover materials

The attenuation of H₂S emissions by various landfill cover materials was evaluated using both laboratory and field experiments. The results demonstrated that cover materials consisting of selected waste products (compost and yard trash) and soils amended with quicklime and calcium carbonate effectively attenuated H₂S emissions and detectable H₂S emissions were only encountered in a testing plot using a sandy soil cover (average emission rate was 4.67 × 10^?6 mg m^?2 s^?1). H₂S concentration profiles in the cover materials indicated that H₂S was removed as it migrated through the cover materials. At the same depth in the testing area, the H₂S concentration in the sandy soil field plot was always higher than that of other testing plots because the sand (a) demonstrated less ability to remove H₂S and (b) exhibited a higher H₂S concentration at the base of the cover. Laboratory experiments confirmed these observations, with a combination of physical adsorption, chemical reactions, and biological oxidation, accounting for the enhanced removal. In addition to removal, the results suggest that some of the cover materials reduced H₂S generation by creating less favorable conditions for sulfate-reducing bacteria (e.g., high pH and temperature).