Genetic biomonitoring of an urban population exposed to mutagenic airborne pollutants

Biomonitoring studies have increased as a consequence of risks and effects to human health on exposure to environmental contaminants, mainly air pollutants. Genetic biomarkers are useful tools for the early assessment of exposure to occupational and environmental pollution. The objective of the present study was to investigate genotoxic effects on people residing and/or working downwind from an oil refinery in southern Brazil and the mutagenic activity of airborne particulate matter (PM10). Samples of peripheral blood and buccal mucosa cells were evaluated using the single-cell gel electrophoresis assay (comet assay) and the micronucleus (MN) assay, respectively. PM10 samples were collected in the target site and the organic matter extraced with dichloromethane was assessed for mutagenic activity in the Salmonella/microsome assay. The exposed group (n = 37) was compared to a reference group (n = 37) of subjects living in an urban area with limited traffic and industrial influence, located far from the main industrial areas. All PM10 organic extracts showed mutagenic positive responses and the effect decreased in the presence of S9 mix indicating that the predominant compounds present were direct-acting mutagens. The responses of YGs strains are consistent with aromatic amines and nitroarenes being present in the PM10 extracts. The group in the area under the influence of the oil refinery (exposed group) showed significantly higher DNA damage in lymphocytes than the reference group. The MN frequencies in buccal mucosa were very low for both groups and no difference between groups was observed. No association was found between age and tobacco smoking habit and level of DNA damages measured by the comet assay. The results indicate that the comet assay was a sensitive tool to detect DNA damage in subjects under the influence of an oil refinery, with marked genotoxic activity in the atmospheric environment.     

Estimating trace gas and aerosol emissions over South America: Relationship between fire radiative energy released and aerosol optical depth observations

Contemporary human activities such as tropical deforestation, land clearing for agriculture, pest control and grassland management lead to biomass burning, which in turn leads to land-cover changes. However, biomass burning emissions are not correctly measured and the methods to assess these emissions form a part of current research area. The traditional methods for estimating aerosols and trace gases released into the atmosphere generally use emission factors associated with fuel loading and moisture characteristics and other parameters that are hard to estimate in near real-time applications. In this paper, fire radiative power (FRP) products were extracted from Moderate Resolution Imaging Spectroradiometer (MODIS) and from the Geostationary Operational Environmental Satellites (GOES) fire products and new South America generic biomes FRE-based smoke aerosol emission coefficients were derived and applied in 2002 South America fire season. The inventory estimated by MODIS and GOES FRP measurements were included in Coupled Aerosol-Tracer Transport model coupled to the Brazilian developments on the Regional Atmospheric Modeling System (CATT-BRAMS) and evaluated with ground truth collected in Large Scale Biosphere–Atmosphere Smoke, Aerosols, Clouds, rainfall, and Climate (SMOCC) and Radiation, Cloud, and Climate Interactions (RaCCI). Although the linear regression showed that GOES FRP overestimates MODIS FRP observations, the use of a common external parameter such as MODIS aerosol optical depth product could minimize the difference between sensors. The relationship between the PM_2.5μm (Particulate Matter with diameter less than 2.5 μm) and CO (Carbon Monoxide) model shows a good agreement with SMOCC/RaCCI data in the general pattern of temporal evolution. The results showed high correlations, with values between 0.80 and 0.95 (significant at 0.5 level by student t test), for the CATT-BRAMS simulations with PM_2.5μm and CO.     

Fuzzy adaptive management of social and ecological carrying capacities for protected areas

Commonly used methods of evaluating the degree of consistency of protected area ecosystems with social and ecological carrying capacities are likely to result in decision errors. This occurs because such methods do not account for imprecision and uncertainty in inferring the degree of ecosystem consistency from an observed ecosystem indicator. This paper proposes a fuzzy adaptive management approach to determine whether a protected area ecosystem is consistent with ecological and social carrying capacities and, if not, to identify management actions that are most likely to achieve consistency when there is uncertainty about the current degree of consistency and how alternative management actions are likely to influence that consistency. The proposed approach is illustrated using a hypothetical example that uses an ecosystem indicator that reflects combinations of different levels of user satisfaction and conservation of threatened and endangered species. Application of the proposed fuzzy adaptive management approach requires a protected area manager to: (1) identify alternative management actions for achieving ecosystem consistency with social and ecological carrying capacities in each of several management zones in a protected area; (2) randomly assign alternative management actions to management zones; (3) define fuzzy sets for the ecosystem indicator and degree of ecosystem consistency, and fuzzy relations between the ecosystem indicator and the degree of ecosystem consistency; (4) monitor the indicator in each management zone; (5) define fuzzy sets based on the observed indicator in each management zone; and (6) combine the fuzzy sets defined on the observed indicator and the fuzzy relations between the indicator and the degree of ecosystem consistency to reach conclusions about the most likely degree of consistency for alternative management actions in each management zone. The fuzzy adaptive management approach proposed here is advantageous when the benefits of avoiding the decision errors inherent with crisp and stochastic decision rules outweigh the added cost of implementing the approach.     

Supporting waste and water management with proactive legal instruments

This study addresses the means local administrators and legislators in emerging economies dispose of to implement directives passed down to them by international environmental agreements. Specific reference is made to agreements and policies dealing with fresh water supply and waste management. Although the Johannesburg Summit asked that the proportion of people without access to drinking water and basic sanitation be halved by 2015, actions need to be developed on a local scale to reach the target. They need to consider the existence of supply limits for water and the subtle difference between sanitation and waste collection. The concepts of reactive and proactive legislation are confronted. Proactive legislation, already used in the industrialized World, is proposed to introduce new patterns of behavior into emerging economy communities. The proposed legal texts have the capacity to be self-fulfilling in the quest for local compliance with international targets.     

Sustaining Ecological Integrity with Respect to Climate Change: A Fuzzy Adaptive Management Approach

A fuzzy adaptive management framework is proposed for evaluating the vulnerability of an ecosystem to losing ecological integrity as a result of climate change in an historical period (ex post evaluation) and selecting the best compensatory management action for reducing potential adverse impacts of future climate change on ecological integrity in a future period (ex ante evaluation). The ex post evaluation uses fuzzy logic to test hypotheses about the extent of past ecosystem vulnerability to losing ecological integrity and the ex ante evaluation uses the fuzzy minimax regret criterion to determine the best compensatory management action for alleviating potential adverse impacts of climate change on ecosystem vulnerability to losing ecological integrity in a future period. The framework accounts for uncertainty regarding: (1) the relationship between ecosystem vulnerability to losing ecological integrity and ecosystem resilience; (2) the relationship between ecosystem resilience and the extent to which observed indicators of ecological integrity depart from their thresholds; (3) the extent of future climate change; and (4) the potential impacts of future climate change on ecological integrity and ecosystem resilience. The adaptive management element of the framework involves using the ex post and ex ante evaluations iteratively in consecutive time segments of the future time period to determine if and when it is beneficial to adjust compensatory management actions to climate change. A constructed example is used to demonstrate the framework.     

Evaluation of an approach for the characterization of reactive and available pools of 20 potentially toxic elements in soils: part II--solid-solution partition relationships and ion activity in soil solutions

To assess environmental risks related to contaminants in soil it is essential to predict the available pool of inorganic contaminants at regional scales, accounting for differences between soils from variable geologic and climatic origins. An approach composed of a well-accepted soil extraction procedure (0.01 M CaCl(2)) and empirical Freundlich-type models in combination with mechanistically based models which to date have been used only in temperate regions was applied to 136 soils from a South European area and evaluated for its possible general use in risk assessment. Empirical models based on reactive element pools and soil properties (pH, organic carbon, clay, total Al, Fe and Mn) provided good estimations of available concentrations for a broad range of contaminants including As, Ba, Cd, Co, Cu, Hg, Mo, Ni, Pb, Sb, Se and Zn (r(2): 0.46-0.89). The variation of the pools of total Al in soils expressed the sorptive capacity of aluminosilicates and Al oxides at the surfaces and edges of clay minerals better than the actual variability of clay contents. The approach has led to recommendations for further research with particular emphasis on the impact of clay on the solubility of As and Sb, on the mechanisms controlling Cr and U availability and on differences in binding properties of soil organic matter from different climatic regions. This study showed that such approach may be included with a good degree of certainty for first step risk assessment procedures to identify potential risk areas for leaching and uptake of inorganic contaminants in different environmental settings.     

Degradation of phenols in olive oil mill wastewater by biological,enzymatic, and photo-Fenton oxidation

Background, aim, and scope  

Olive oil mill wastewater (OOMW) environmental impacts minimization have been attempted by developing more effective processes, but no chemical or biological treatments were found to be totally effective to mitigate their impact on receiving systems. This work is the first that reports simultaneously the efficiency of three different approaches: biological treatment by two fungal species (Trametes versicolor or Pleurotus sajor caju), enzymatic treatment by laccase, and chemical treatment by photo-Fenton oxidation on phenols removal.     

Monitoring of ambient air PCDD/F levels in Portugal

As part of a monitoring program conducted by IDAD--Institute for Environment and Development and supported by regional municipal solid waste (MSW) management authorities, dioxin concentrations in ambient air were measured in three regions of Portugal: Porto, Lisbon and Madeira. These independent studies were performed with the intention of providing data as a basis for the evaluation of potential impacts of the operation of recently built MSW incinerators. Thus, 170 samples were collected in nine different sites from January 1999 till present. The measured levels revealed an extremely variable content of PCDDs/PCDFs depending both on the area and the season of the year. Samples taken in Porto and Lisbon reveal a similar homologue structure even if concentrations measured in the Porto region are significantly greater. Data from Madeira is characteristic of a remote site with some of the congeners concentrations below the detection limit.     

Fluorescence and DOC contents of estuarine pore waters from colonized and non-colonized sediments: effects of sampling preservation

The influence of the colonization of salt marsh sediments with Halimione portulacoides was evaluated by analysing the fluorescent dissolved organic matter (FDOM) in sediment pore waters from a salt marsh at different depths. Cores of sediments at colonized and non-colonized sites were collected from a coastal lagoon (Ria de Aveiro, Portugal). The DOC content of extracted pore waters was determined and characterized by synchronous molecular fluorescence (Deltalambda=60nm) and UV-visible spectroscopies. The common practice of freezing sediment cores for further and later chemical investigation was shown not to be an appropriate methodology of sample preservation. On the contrary, freezing of extracted and filtered pore water seemed not to affect either the DOC content or the fluorescence properties of pore waters. Two types of fluorescent substances were found in the pore waters spectra; one corresponding to humic-like substances and another one resembling proteins. However, major differences were found in the spectra of pore waters depending on both depth and the presence/absence of vegetation colonization.     

Offshore drilling effects in Brazilian SE marine sediments: a meta-analytical approach

The exploration and production of oil and gas reserves often result to drill cutting accumulations on the seafloor adjacent to drill locations. In this study, the detection of drilling influence on marine sediments was performed by meta-analytical comparison between data from pre- and post-drilling surveys undertaken in offshore Campos Basin, southeast of Brazil. Besides this overall appraisal on the geochemical variables, a multivariate assessment, considering only the post-drilling data, was performed. Among the variables, fines content, carbonates, total organic carbon, barium, chromium, copper, iron, manganese, nickel, lead, vanadium, zinc, and total petroleum hydrocarbons, only barium, copper, and hydrocarbons were related to drilling impacts. In relation to the point of discharge, relative elevated levels in the post-drilling campaigns were observed preferentially up to 500 m in the northeast and southwest directions, associated to the Brazil Current-predominant direction. Other distributed concentrations in the surroundings seem to indicate the dilution and dispersion of drilling waste promoted by meteoceanographic factors.