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211.
Cadmium stress alters gene expression of DNA mismatch repair related genes in Arabidopsis seedlings 总被引:1,自引:0,他引:1
Cadmium (Cd) is a non essential element, and is a widespread environmental pollutant. Exposure to Cd can result in a variety of adverse health effects in plant and humans. In the current study, Arabidopsis seedlings were used as a bio-indicator of Cd pollution. Seedlings were grown on MS media containing 0-6.0 mg L(-1) Cd for 18 days, and the gene expression patterns were used to link increased Cd exposure with progressive biological effects. Reduction of total soluble protein content in shoots of the Arabidopsis seedlings occurred with increase in Cd concentrations. For the gene expression patterns, seven genes known to be involved in cell division and DNA mismatch repair (MMR) system were investigated by semi-quantitative RT-PCR, and normalized using 18S rRNA gene expression. Expression of the proliferating cell nuclear antigen 2 (atPCNA 2), MutS 3 homolog (atMSH 3) and MutL1 homolog (atMLH1) genes in shoots of Arabidopsis was strongly induced by exposure to 0.75 mg L(-1) Cd, but were repressed by other Cd concentrations whereas exposure to 0.75-6 mg L(-1) of Cd resulted in a decreased expression of atPCNA1, atMSH 2, 6 and 7 genes independently of any observable biological effects, including survival, fresh weight and chlorophyll level of shoots. This work demonstrated that specific gene expression changes could serve as useful molecular biomarkers indicative of Cd exposure and related biological effects. 相似文献
212.
Various developmental stages (early larvae to adults) of Euphausia superba have been collected in different seasons in the Weddell Sea, the Lazarev Sea and off the Antarctic Peninsula to investigate
the role of lipids and fatty acids in the life cycle of the Antarctic krill. The total-lipid data for E. superba exhibited seasonal variations, with low lipid levels in late winter/early spring and the highest levels in autumn. Seasonal
changes were most pronounced in the immature and adult specimens, increasing from about 10% lipid of dry mass to more than
40%. The fatty-acid compositions of the younger stages were dominated by 20:5(n-3), 22:6(n-3) and 16:0. These are typical
phospholipid fatty acids, which are major biomembrane constituents. The phospholipid composition was similar in the older
stages. With increasing storage of triacylglycerols in the lipid-rich immature and adult stages, the fatty acids 14:0, 16:0
and 18:1(n-9) prevailed, comprising about 70% of total triacylglycerol fatty acids. The trophic-marker fatty acids 16:1(n-7)
and 18:4(n-3), indicating phytoplankton ingestion, were less abundant. They reflected, however, the dependence of the larvae
on phytoplankton as well as the seasonal changes in algal composition. The generally close linear relationships between fatty
acids and lipid suggest that the fatty-acid compositions of the collected specimens were largely independent of the respective
developmental stage, season and region. The linear fit indicates that triacylglycerol accumulation started at a level of about
5% of total lipid. Considering the various overwintering scenarios under discussion, the life cycle and reproductive strategies
of krill are discussed in the context of the lipid metabolism and fatty-acid composition of E. superba. Lipid production is effective enough to accumulate large energy reserves for the dark season, but E. superba does not exhibit the sophisticated biosynthetic pathways known from other Antarctic euphausiids and copepods. Although important,
lipid utilisation appears to be just one of several strategies of E. superba to thrive under the extreme Antarctic conditions, and this pronounced versatility may explain the success of this species
in the Southern Ocean.
Received: 16 June 2000 / Accepted: 18 December 2000 相似文献
213.
Juvenile and adult marine organisms differ in their morphology, chemistry, physiology, behavior, and ecology. Because juvenile
algae are thinner, smaller, and have more delicate tissues than adults, they are often assumed to be more susceptible to grazers.
We examined within-species food preferences of four common generalist herbivores for juvenile and adult tissues of eight common
brown algae in two-choice laboratory food-preference experiments. Our results showed that juvenile algae did not tend to be
a preferred food of herbivores. Juvenile tissues were significantly preferred over adult tissues in only four of the 32 combinations
of algae and herbivores tested. In 12 experiments, adult tissues were preferred over juvenile tissues, and no choice occurred
in the remaining 16 experiments. When sea urchins exhibited a preference, it was always for adult tissues. The other three
herbivores, an isopod and two snails, were more variable in their choices, sometimes preferring juveniles, sometimes adults,
and sometimes having no preference. We measured nitrogen and phlorotannin concentrations in adult and juvenile seaweeds to
see whether these parameters were correlated with herbivore food preferences. Nitrogen levels were similar in juveniles and
adults of three algal species and were higher in juveniles of two. Phlorotannin levels were higher in juveniles of four species
and lower in juveniles of one. The other three species showed no differences in phlorotannin levels. Phlorotannin concentrations
decreased with increasing juvenile size in three species and increased with increasing size in one species. Neither nitrogen
nor phlorotannin concentrations explained overall herbivore food preferences for algae of different stages. Our results suggest
that preferences of certain grazers for juvenile algae are not as strong as previously assumed and are dependent on herbivore
species. Preferences between juveniles and adults are likely to be determined by a combination of morphological and chemical
features of the tissues and the unique responses of herbivore species to those features.
Received: 10 April 2000 / Accepted: 19 November 2000 相似文献
214.
Potential dietary effects on the fatty acid composition of the common jellyfish Aurelia aurita 总被引:2,自引:0,他引:2
Fatty acid composition of the natural and aquarium-reared common jellyfish Aurelia aurita was investigated. Fatty acid composition of the aquarium-reared A. aurita clearly reflected that of the diet, brine shrimp (Artemia). In the same way, fatty acid composition of the natural A. aurita was assumed to reflect those of natural diets. Samples of natural A. aurita were collected from April 1995 to September 1995 in the Seto Inland Sea, Japan, and their fatty acids were analyzed by gas
chromatography and mass spectrometry. Variation of fatty acid compositions was seasonal rather than dependent on body size.
Two major seasonal groups were divided by the cluster analysis of the A. aurita fatty acid composition: the April–June and the August–September clusters. The April–June cluster was characterized by high
contents of the (n − 3)-fatty acids of diatom origin, accumulated via the grazing food chain. By contrast, the August–September cluster was
characterized by an increase in (n − 6)-fatty acids of macroalgal origin, probably transferred via the detritus food chain. These results suggest that the diet
of natural A. aurita may shift between the diatom-based food chain and the detritus-based food chain.
Received: 12 April 2000 / Accepted: 1 December 2000 相似文献
215.
D. Kim T. Okamoto T. Oda K. Tachibana K.S. Lee A. Ishimatsu Y. Matsuyama T. Honjo T. Muramatsu 《Marine Biology》2001,139(4):625-632
Although the ichthyotoxic mechanism of Chattonella marina is still unknown, several lines of evidence suggest that the reactive oxygen species (ROS), such as superoxide anion (O2-), hydrogen peroxide (H2O2), and hydroxyl radical (·OH), produced by C. marina are involved in the mortality of fish exposed to this flagellate. Recently, we found that the cell-free supernatant prepared from C. marina, which is considered to contain the glycocalyx, showed NADPH-dependent O2- generation. In this study, we prepared antiserum against the crude glycocalyx of C. marina. Using indirect immunofluorescence, it was confirmed that the antiserum specifically reacted with C. marina cells. In addition to C. marina, the antiserum also reacted with other raphidophycean flagellates such as Heterosigma akashiwo, Olisthodiscus luteus, and Fibrocapsa japonica, whereas no reactivity was observed against six other flagellate species tested. These results suggest that raphidophycean flagellates have common epitopes recognized by the antiserum. Interestingly, immunohistochemical analysis of paraformaldehyde-fixed gill lamellae from yellowtail exposed to C. marina revealed that the antiserum stained the surface of gill lamellae, while no such staining pattern was observed in control gill lamellae. These results suggest that the glycocalyx may be discharged when C. marina cells are inhaled into the fishes' mouths and then come into contact with the gill surface. Based on the present results, together with our previous findings, we propose that continuous accumulation of the discharged glycocalyx on the gill surface occurs during C. marina exposure, which may be responsible for the ROS-mediated severe gill tissue damage leading to fish death. 相似文献
216.
A distinct smell of dimethylsulfide (DMS) was noted at the edge of the intertidal mudflat of Marennes-Oléron Bay, at the
French Atlantic coast, where dense populations of the marine flatworm Convoluta roscoffensis Graff (Platyhelminthes: Turbellaria) were present. DMS is the cleavage product of dimethylsulfoniopropionate (DMSP). DMSP
was shown to be present in high amounts in sediment containing the flatworm as well as in axenic cultures of the symbiotic
alga Tetraselmis sp. that was isolated from the flatworm. In untreated sediment samples containing C. roscoffensis the concentration of DMS was as high as ∼55 μmol l−1 sediment, and in samples that were fixed with glutaraldehyde the concentration of DMS was even three orders of magnitude
higher (∼66 mmol l−1 sediment). This rapid cleavage of DMSP to DMS in fixed samples was unexpected. Pure DMSP was stable in glutaraldehyde, and
it was therefore concluded that a DMSP-lyase was responsible for cleavage in the field samples. The isolated symbiotic alga,
Tetraselmis sp., did not show DMSP-lyase activity, indicating that DMSP-lyase may have been present in the flatworm, although the role
of bacteria could not be excluded. The Chl a-specific DMSP content of C. roscoffensis (∼200 mmol g−1) was much higher than that of Tetraselmis sp. (∼30 mmol g−1). Possibly, DMSP was not only present in the symbiotic alga, but was also incorporated in the body tissue of the flatworm.
It remains unclear what the function of DMSP is in C. roscoffensis. In Tetraselmis sp., but not in C. roscoffensis, DMSP increased with increasing salinity. It was concluded that salinity probably does not play an important role in the
dynamics of DMSP and DMS in sediment containing C. roscoffensis.
Received: 21 January 2000 / Accepted: 29 August 2000 相似文献
217.
218.
Human population growth in coastal areas continues to threaten estuarine ecosystems and resources. Populations of Crassostrea virginica have declined across the USA due to water quality degradation, disease pressure, alteration of habitat, and other changes related to anthropogenic impacts. Metals that may be present in estuarine habitats can bioaccumulate in oysters, with potential consequences to the health of oysters and humans consumers. This study (1) evaluated the occurrence and relationships of metal concentrations in oyster tissue versus estuarine sediments, (2) examined oyster tissue concentrations in relation to state water quality designations, and (3) evaluated the potential risk for humans from oyster consumption related to metal concentrations from harvestable waters. Results indicated metal concentrations in sediments and oysters along coastal South Carolina remain low compared to other areas and that concentrations in oyster tissue and adjacent sediments were not highly correlated with each other. However, high concentrations of some metals occurred in oysters sampled from areas designated as Approved for Harvesting. This is important because most harvest area designation systems rely on regular bacterial monitoring when evaluating the safety of consumption. Others safety measurements may be necessary as part of routine monitoring. 相似文献
219.
H. W. Zhang Y. Q. Sun Y. Li X. D. Zhou X. Z. Tang P. Yi A. Murad S. Hussein D. Alshamsi A. Aldahan Z. B. Yu X. G. Chen V. d. P. MUGWANEZA 《Environmental monitoring and assessment》2017,189(8):411
Assessment of groundwater quality plays a significant role in the utilization of the scarce water resources globally and especially in arid regions. The increasing abstraction together with man-made contamination and seawater intrusion have strongly affected groundwater quality in the Arabia Peninsula, exemplified by the investigation given here from the United Arab Emirates, where the groundwater is seldom reviewed and assessed. In the aim of assessing current groundwater quality, we here present a comparison of chemical data linked to aquifers types. The results reveal that most of the investigated groundwater is not suitable for drinking, household, and agricultural purposes following the WHO permissible limits. Aquifer composition and climate have vital control on the water quality, with the carbonate aquifers contain the least potable water compared to the ophiolites and Quaternary clastics. Seawater intrusion along coastal regions has deteriorated the water quality and the phenomenon may become more intensive with future warming climate and rising sea level. 相似文献