Speciations of trace metals in the Danube alluvial sediments within an oil refinery

A sequential extraction procedure was applied to identify forms of Ni, Zn, Pb and Cu with Fe- and Mn-oxides associated in alluvial sediments of the River Danube within Pancevo Oil Refinery (Serbia). The five steps of the sequential extraction procedure partitioned metals into: CH(3)COONH(4) extractable (S1); NH(2)OH.HCl carbonate extractable and easily reducible (S2); (NH(4))(2)C(2)O(2)/H(2)C(2)O(2) moderately reducible (S3); H(2)O(2)-HNO(3) organic extractable (S4); and HCl acid soluble residue (S5). Extracted concentrations of trace metals, analyzed after all five steps, were found to be (mg kg(-1)) for Mn: 656, Fe: 26734, Ni: 32.3, Zn: 72.8, Pb: 13.4 and Cu: 27.0. Most of the elements were found in acid soluble residue, characterizing stable compounds in sediments. Non-residual fractions of trace metals (sum of the first four fractions) were analyzed because they are more bioavailable than the residual amount. Correlation analysis and two multivariate analysis methods (principal component and cluster analysis) were used to understand and visualize the associations between the non-residual fractions of trace metals and certain forms, more or less crystalline of Fe- and Mn-oxides within the analyzed sediments, since Fe- and Mn-oxides play an important role in trace metal sorption within aquatic systems, especially within the Danube alluvium where the fluctuations of groundwater are very frequent and the level of groundwater could come close to surface.     

Long-term variations of folic acid concentrations in the Northern Adriatic

Folic acid (FA), which belongs to B-group vitamins has recently been recognized as possible nutrient for some phytoplankton species (a diatom Phaeodactylum tricornutum). The beneficial role of FA in human health is widely documented. In some countries food fortification with FA has already become mandatory. FA is also used under commercial name of Dosfolat for the treatment of industrial and domestic wastewaters. This is an exploratory study on seasonal and depth variability of FA concentrations in the Northern Adriatic Sea (station 101-max depth 30 m, under the Po River influence). Seawater samples were analysed in the period from February 1998 to February 2003 based on monthly determinations. Those preliminary findings showed that in the upper layer (10 m) FA concentrations were in the range from 0.1 to 28 nM. In the lower layer (20-30 m) FA concentrations were in the range from 0.1 to 12 nM. It was found that phytoplankton and microorganisms which are responsible for the production of FA in the Northern Adriatic (Station 101) were situated at the depth from 5 to 10 m. The observed water column and seasonal distributions depended on a sensitive balance between the production and the consumption of FA.     

Levels of elements in the surficial estuarine sediments of the Hugli River, northeast India and their environmental implications

The present paper is the first document of a detailed geochemical and mineralogical study of muddy to sandy mud estuarine sediments of the Hugli River collected from five different sites along its course in the coastal areas of West Bengal, northeast India. The present work attempts to establish the status of distribution and environmental implications of 52 elements in the surficial estuarine sediments and their possible sources of derivation. The level of both metallic and non-metallic elements shows a wide range of variation all along the course of the estuary and can be attributed to their differential derivation from the source rocks and differential discharge of untreated effluents originating from industrial, agricultural, aquacultural as well as domestic sewage. The element contents, particularly the heavy metal content in the sediments, are the lowest in the upstream part of the estuary at Diamond Harbor, whereas, these are slightly higher in the intermediate stretch of the estuary at Haldia to highest in the mouth of estuary at Gangasagar. These changes indicate that the metals that are carried from upstream find their ultimate depositional sink at the delta mouth near Gangasagar, where almost all the elements showed elevated values. The majority of the elements have their highest concentrations at Canning, a site within the tidal channel network of the Hugli-Matla drainage basin away from the direct influence of the Hugli River. This site is severely contaminated with huge organic load from domestic sewage, aquaculture, intensive trawling activities and agricultural runoff. Moreover, the site suffers from heavy siltation load causing an almost moribund condition of Matla River at this point. Equi-dimensional quartzo-feldspathic mineral grains are consistent components in the siliciclastic composition of the sediments and their sizes at each station are controlled by respective hydrodynamic conditions. Various flaky minerals (mica, chlorite, hornblende) are also present but show inconsistent patterns of distribution. These flaky minerals remain in suspension for a long time and do not follow the usual law of settling of particles depending on the hydrodynamic regime prevailing in the area. Presented data will serve as a baseline against which future anthropogenic effects may be assessed. A comprehensive account of heavy metal content in sediments from different coastal regions of peninsular India has also been presented for purpose of comparison.     

Direct and indirect trophic interactions of <Emphasis Type="Italic">Aurelia </Emphasis>sp. (Scyphozoa) in a stratified marine environment (Mljet Lakes,Adriatic Sea)

The pattern of diel vertical migration and the trophic interactions of moon jelly (Aurelia sp.) were investigated in the sea lakes of Mljet Island (Adriatic Sea) where this scyphomedusa is present throughout the year. Water column characteristics, plankton and in situ behaviour of Aurelia were followed over several 24-h cycles (6–8 times during each cycle) from the surface to the bottom (44 m). Aurelia exhibited a consistent pattern of diel vertical migration. Most of the time Aurelia were located at the bottom of the thermocline layer at temperatures lower than 19°C. Aurelia migrated towards the surface at dusk when the majority was found within the thermocline or just above it. During the night the medusae sank into the deepest layers below 25 m. The main medusa food items inferred from stomach contents were small adult copepods like Oithona nana and Paracalanus parvus and copepodites of small calanoids and cyclopids. In addition, in situ feeding experiments indicated high clearance rates for nauplii and naked ciliates and clear response of bacterial populations pointing to indirect cascade effects of Aurelia on microbial in addition to classical food web.