Graphene quantum dots(GQDs) possess great potential in various applications due to their superior physicochemical properties and wide array of available surface modifications.However, the toxicity of GQDs has not been systematically assessed, thus hindered their further development; especially, the risk of surface modifications of GQDs is largely unknown. In this study, we employed a lung carcinoma A549 cells as the model to investigate the cytotoxicity and autophagy induction of three types GQDs, including cGQDs(COOH-GQDs), hGQDs(OH-GQDs), and aGQDs(NH_2-GQDs). The results showed hGQDs was the most toxic, as significant cell death was induced at the concentration of 100 μg/mL,determining by WST-1 assay as well as Annexin-V-FITC/PI apoptosis analysis, whereas cGQDs and aGQDs were non-cytotoxic within the measured concentration. Autophagy detection was performed by TEM examination, LC3 fluorescence tracking, and Westernblot. Both aGQDs and hGQDs induced cellular autophagy to various degrees except for cGQDs. Further analysis on autophagy pathways indicated all GQDs significantly activated p-p38 MAPK; p-ERK1/2 was inhibited by aGQDs and hGQDs but activated by c GQDs. p-JNK was inhibited by aGQDs and c GQDs, while activated by hGQDs. Simultaneously, Akt was activated by hGQDs but inhibited by aGQDs. Inhibition of autophagy by 3-MA significantly increased the cytotoxicity of GQDs, suggesting that autophagy played a protective role against the toxicity of GQDs. In conclusion, c GQDs showed excellent biocompatibility and may be considered for biological applications. Autophagy induction may be included in the health risk assessment of GQDs as it reflects the stress status which may eventually lead to diseases. 相似文献
Environmental Chemistry Letters - Microalgae are photosynthetic cell factories of global interest for fuels, food, feed, bioproducts, carbon sequestration, waste mitigation, and environmental... 相似文献
As China’s economy began transitioning from one focused on high-speed growth to one focusing on high-quality development, sustainable green development has become the main goal pursued by the government. This study empirically measures the marginal impact of per capita GDP, technological innovation level, industrial structure, openness, fiscal decentralization, and urbanization level on per capita wastewater discharge in 11 provinces (cities) along the Yangtze River Economic Belt (YREB) from 2008 to 2018 using a quantile model. The key findings were as follows: (1) factors such as the per capita GDP, industrial structure, foreign direct investment, and urbanization in the YREB significantly increased water resource pollution; (2) the quantile model regression results showed that the relationship between economic growth and ecological pollution followed the so-called environmental Kuznets inverted U-curve. Wastewater discharge per capita was low in areas with low per capita GDP, meaning that the ecological environment in these areas was more fragile and that the environmental pollution costs due to economic growth were therefore relatively much higher in these areas; (3) fiscal decentralization significantly reduced water resource pollution in relatively developed areas although the effects in the relatively developing areas were not significant; and (4) the effects of technological innovation on reducing water resource pollution in the YREB were positive but not very significant. The results also confirmed that traditional patterns of economic growth increased water pollution in the YREB. For this reason, the government needs to urgently improve policies—for example, upgrading economic structures, preventing over-urbanization, speeding up technological innovation, introducing environmentally friendly foreign investment, and providing more rewards to best practitioners of environmental governance—that is conducive to the achievement of green ecological development.
Environmental Science and Pollution Research - In this study, we investigated the impact of the mixed-application with pymetrozine on the behavior (i.e., uptake, translocation, and degradation) of... 相似文献
With the rapid economic development and urbanization, there would be severe air pollution. The path analysis method was employed to investigate the specific effects of Industrial Gross Domestic Product (IGDP), Urbanization Rate (UR), Environmental Investment (EI), and Coal Consumption (CC) on Industrial SO2 Emissions (ISE) and Industrial Dust Emissions (IDE). The results shown that (1) IGDP is the main reason for the increase in ISE and IDE, with the direct positive influence more than 20% and a total contribution of more than 35%, and the combined effects of UR can reduce air pollutants emission considerably, especially in ISE. (2) The effects of EI and CC on ISE could be ignored, given that their contributions comprised <?10%. Meanwhile, the contribution of EI to IDE was only 8.92%. For one reason, EI is underfunded which the percentage of Gross Domestic Product was <?2%. 相似文献