Programmed cell death induced by modified clay in controlling Prorocentrum donghaiense bloom

Modified clay (MC), an effective material used for the emergency elimination of algal blooms, can rapidly reduce the biomass of harmful algal blooms (HABs) via flocculation. After that, MC can still control bloom population through indirect effects such as oxidative stress, which was initially proposed to be related to programmed cell death (PCD) at molecular level. To further study the MC induced cell death in residual bloom organisms, especially identifying PCD process, we studied the physiological state of the residual Prorocentrum donghaiense. The experimental results showed that flocculation changed the physiological state of the residual cells, as evidenced by growth inhibition and increased reactive oxygen species production. Moreover, this research provides biochemical and ultrastructural evidence showing that MC induces PCD in P. donghaiense. Nuclear changes were observed, and increased caspase-like activity, externalization of phosphatidylserine and DNA fragmentation were detected in MC-treated groups and quantified. And the mitochondrial apoptosis pathway was activated in both MC-treated groups. Besides, the features of MC-induced PCD in a unicellular organism were summarized and its concentration dependent manner was proved. All our preliminary results elucidate the mechanism through which MC can further control HABs by inducing PCD and suggest a promising application of PCD in bloom control.     

Enhanced the photoelectrochemical performance of Bi2XO6 (X = W, Mo) for detecting hexavalent chromium by modification of CuS

In this work, Bi₂XO₆ (X = W, Mo) are synthesized at different temperatures. The results of tests find the optimal temperatures of Bi₂WO₆ and Bi₂MoO₆ are 180 and 160°C (BW-180, BM-160). Then, BW-180 and BM-160 are further compounded with different contents of CuS. The results of photoelectrochemical (PEC) tests show that CuS can improve the PEC performance of semiconductor materials, and it has better performance when CuS mass fraction is 5%. These maybe the photoelectron potentials generated by CuS/Bi₂XO₆ (X = Mo, W) heterojunction reduce the combination of photogenerated electrons and holes. When the PEC sensor based on 5%-CuS/BW-180 detects Cr(VI), it has a linear range of 1–80 μmol/L with detection limit of 0.95 μmol/L, while the PEC sensor based on 5%-CuS/BM-160 detects Cr(VI) has a linear range of 0.5–230 μmol/L and a detection limit of 0.12 μmol/L. Thus, 5%-CuS/Bi₂XO₆ has potential application in hexavalent chromium detection.     

Contents and sources of polycyclic aromatic hydrocarbons and organochlorine pesticides in vegetable soils of Guangzhou, China

We investigated contents, distribution and possible sources of PAHs and organochlorine pesticides (Ops) in 43 surface and subsurface soils around the urban Guangzhou where variable kinds of vegetables are grown. The results indicate that the contents of PAHs (16 US EPA priority PAHs) range from 42 to 3077 microg/kg and the pollution extent is classified as a moderate level in comparison with other investigations and soil quality standards. The ratios of methylphenanthrenes to phenanthrene(MP/P), anthracene to anthracene plus phenanthrene (An/178), benz[a]anthracene to benz[a]anthracene plus chrysene (BaA/228), indeno[1,2,3-cd]pyrene to indeno[1,2,3-cd]pyrene plus benzo[ghi]perylene (In/In+BP) suggest that the sources of PAHs in the soil samples are mixed with a dominant contribution from petroleum and combustion of fossil fuel. The correlation analysis shows that the PAHs contents are significantly related to total organic carbon contents (TOC) (R2=0.75) and black carbon contents (BC) (R2=0.62) in the soil samples. Dichlorodiphenyltrichloroethane and metabolites (DDTs) and hexachlorocyclohexanes and metabolites (HCHs) account largely for the contaminants of OPs. The concentrations of DDTs range from 3.58 to 831 microg/kg and the ratios for DDT/(DDD+DDE) are higher than 2 in some soil samples, suggesting that DDT contamination still exists and may be caused by its persistence in soils and/or impurity in the pesticide dicofol. The concentrations of HCHs are 0.19-42.3 microg/kg.     

Fabrication of PVA/GO Nanofiber Films by Electrospinning: Application for the Adsorption of Cu2+ and Organic Dyes

Journal of Polymers and the Environment - In recent years, the treatment of water pollution has been a challenging and far-reaching topic. In order to improve the adsorption performance of...     

珠三角城市群人居环境高质量发展时空演化特征及影响因素

综合运用熵权法、协调度研究了2010—2019年珠三角城市群人居环境高质量发展时空演化特征,并采用障碍度分析了该城市群影响人居环境高质量发展的障碍因子。结果表明:①珠三角城市群人居环境高质量水平呈上升的态势,空间分布呈“屋脊”式格局,区域差异明显。②在人居环境高质量五大准则层中,除生态环境外,其他四大准则层均表现为波动上升态势,协调度呈现高质量低协调和低质量高协调两种差异。③公共基础服务、社会经济和居住环境对珠三角城市群人居环境高质量发展障碍度较大。     

Comparison of macroinvertebrate community structure in urban (Shenzhen City) streams with typical habitat characteristics北大核心CSCD

2020年12月在深圳市城市区域典型生境特征溪流——城市山区源头溪流金龟河、国家地质公园保护区入海溪流杨梅坑河与黑臭水体治理后溪流石溪河,开展底栖动物多样性与生境质量状况取样,通过多元统计分析探明各溪流底栖动物群落结构差异及其主要环境影响因子.结果表明:(1)金龟河底质异质性(SI)最高;杨梅坑河水质最好且流态异质性(Fr)最优,石溪河水质最差且底质异质性(Fr)最低.(2)共采集鉴定大型底栖动物118个分类单元,隶属于3门6纲14目87科.水生昆虫均占绝对优势,其中金龟河67属(81.7%),杨梅坑河62属(95.4%),石溪河12属(52.2%).金龟河中底栖动物Shannon-Wiener多样性指数、改进的Shannon-Wiener多样性指数、Pielou均匀度均高于杨梅坑河与石溪河.(3)金龟河的主要功能摄食类群是滤食者(45.5%),杨梅坑河(38.3%)与石溪河(58.7%)的主要功能摄食类群均为收集者.(4)冗余分析(RDA)结果显示,氨氮、海拔、湿宽、水温、溶氧、底质异质性(SI)、电导率和流态异质性(Fr)为影响深圳市溪流的大型底栖动物群落结构的主要环境因子.研究表明:(1)深圳市的山区溪流可作为深圳市生物多样性保护的重点区域.(2)在城市溪流生态系统中,水环境化学因子并非为主要影响因子,生境多样性发挥了重要作用.(图6表3参43)     

Stoichiometric characteristics of carbon,nitrogen, and phosphorus in leaf litter soil of Pinus yunnanensis forest during different restoration stages in the karst plateau of eastern Yunnan北大核心CSCD

以滇东岩溶坡地不同恢复阶段云南松林(纯林、人工混交林、天然次生林)为研究对象,以元江栲原生林和小铁仔灌丛作为参照样地,对5种植被类型中的叶片-枯落物-土壤中的C、N、P含量和化学计量比特征进行研究.结果表明:(1)3种云南松林都呈现为高C(432.27 g/kg)、低N(10.28 g/kg)、P(0.96 g/kg)的格局,5种植被类型的叶片-枯落物-土壤C、N、P含量基本都表现为叶片>枯落物>土壤,C/N、C/P、N/P值则都表现为枯落物>叶片>土壤,叶片和枯落物的养分含量和化学计量比值与土壤间差异显著.(2)3种云南松林对于养分的吸收同化能力差异不大,但天然次生林的枯落物质量最好,人工混交林的土壤N、P有效性最高,云南松林内受N的胁迫作用强于原生林和灌丛.(3)植物叶片-枯落物-土壤中C、N、P及其化学计量比间相关性显著,互馈机制明显.研究区内土壤C、N、P化学计量特征受土壤pH、团聚体颗粒、含水率、容重和硝态氮影响显著.因此,滇东岩溶高原云南松植被恢复过程中主要受N胁迫作用,提高枯落物养分回流是云南松植被恢复与经营的关键要素.(图4表3参41)     

Effects of bedform migration on nutrient fluxes at the sediment–water interface: a theoretical analysis

Environmental Fluid Mechanics - Nutrient fluxes at the sediment–water interface are essential for water quality and aquatic ecosystems. In this study, a unified expression for the sediment...     

Influence of extracellular polymeric substances from activated sludge on the aggregation kinetics of silver and silver sulfide nanoparticles

• The NPs aggregation in the electrolyte solution is consistent with the DLVO theory. • In NaNO₃ and low Ca(NO₃)₂, EPS alleviates the NPs aggregation by steric repulsion. • In high Ca(NO₃)₂, EPS accelerates the NPs aggregation by exopolysaccharide bridging. • Ag₂S NPs have stronger stability compared with Cit-Ag NPs in aqueous systems. Extracellular polymeric substances (EPS) in activated sludge from wastewater treatment plants (WWTPs) could affect interactions between nanoparticles and alter their migration behavior. The influence mechanisms of silver nanoparticles (Ag NPs) and silver sulfide nanoparticles (Ag₂S NPs) aggregated by active EPS sludge were studied in monovalent or divalent cation solutions. The aggregation behaviors of the NPs without EPS followed the Derjaguin-Landau-Verwey-Overbeek (DLVO) theory. The counterions aggravated the aggregation of both NPs, and the divalent cation had a strong neutralizing effect due to the decrease in electrostatic repulsive force. Through extended DLVO (EDLVO) model analysis, in NaNO₃ and low-concentration Ca(NO₃)₂ (<10 mmol/L) solutions, EPS could alleviate the aggregation behaviors of Cit-Ag NPs and Ag₂S NPs due to the enhancement of steric repulsive forces. At high concentrations of Ca(NO₃)₂ (10‒100 mmol/L), exopolysaccharide macromolecules could promote the aggregation of Cit-Ag NPs and Ag₂S NPs by interparticle bridging. As the final transformation form of Ag NPs in water environments, Ag₂S NPs had better stability, possibly due to their small van der Waals forces and their strong steric repulsive forces. It is essential to elucidate the surface mechanisms between EPS and NPs to understand the different fates of metal-based and metal-sulfide NPs in WWTP systems.