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Characterization and phylogenetic analysis of a phenanthrene-degrading strain isolated from oil-contaminated soil 总被引:2,自引:0,他引:2
Bacterium strain EVA17 was isolated from an oil-contaminated soil, and identified as Sphingomonas sp.based on analysis of 16S rDNA sequence, cellular fatty acid composition and physiological-chemical tests. The salicylate hydroxylase and catechol 2, 3-dioxygenase (C23O) were detected in cell-free lysates, suggesting a pathway for phenanthrene catabolism via salicylate and catechol. Alignment showed that both of the C23O and GST genes of the strain EVA17 had high similarity with homologues of strains from genus Sphingomonas. The phylogenetic analysis based on 16S rDNA and C23O gene sequence indicated that EVA17 should be classified into genus Sphingomonas, although the two phylogenetic trees were slightly different from each other. The results of co-amplification and sequence determination indicated that GST gene should be located upstream of the C230 gene. 相似文献
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根据“十五”、“十一五”期间“创模”指标体系,分析了各阶段指标体系差异和变化,而最新的指标体系能更全面地反映环境质量。同时,“创模”指标的变化趋势分析表明,“创模”指标的发展必然是反映当时的环保工作重点,“创模”的基本条件门槛会不断提升,“创模”指标的内涵会不断丰富,以确保国家环境保护模范城市的先进性和示范性。 相似文献
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Ann Verdoodt Stephen M. Mureithi Liming Ye Eric Van Ranst 《Agriculture, ecosystems & environment》2009,129(1-3):332-339
The establishment of enclosures has become an important measure to combat land degradation in many of the world's semi-arid rangelands. In view of the increased pressure exerted by this land reclamation strategy on the neighbouring agricultural lands, knowledge of the time required for restoring vegetation cover and soil health, and of the potential positive impact of an adapted management strategy, is highly required. This paper assesses the vegetation and soil rehabilitation in a 23-year chronosequence of two different enclosure management types. In the severely degraded, semi-arid Njemps Flats plain of the Lake Baringo Basin in Kenya communal enclosures characterised by high quality inputs and strict control, and private enclosures managed by individual farmers, were installed since the 1980s. Six communal enclosures (3–17 years since establishment) and six private enclosures (13–23 years since establishment) were selected. Vegetation cover was estimated along three 50 m transects set within each enclosure and in the adjacent open grazing area using the point-to-line transect method. Five 0.5 m2 quadrats systematically placed alongside each transect were sampled for herbaceous standing biomass and topsoil physical, chemical and biological analyses. Grass cover and herbaceous biomass production proved to be the most responsive biotic parameters under both management types, whereas the recovery of the forbs was unsuccessful. Under communal management, the biomass production fully recovered up to its optimal level as recorded in the neighbouring nature reserves. Within private enclosures however, the adopted management strategies seriously restricted biomass production to a significantly lower level. Soil quality generally recovered more slowly with time. Significant improvements compared to the open rangeland were recorded in topsoil bulk density, organic C and total N stocks, and microbial biomass C and N stocks of the communal enclosures. Unlike the communal enclosures, only topsoil bulk density and the microbial biomass C stock showed a significant difference in the private enclosures. With respect to C and total N stocks, and the microbial biomass N a non-significant improving trend was recorded. The level of chemical and biological soil quality obtained under both management types is still low and draws the attention to the importance of careful monitoring of grazing and grass cutting activities under both enclosure management strategies. The chronosequences further highlight the potential of some well-managed private enclosures, whereas intrinsic soil properties such as high alkalinity, as well as changes in management, limit the rehabilitation of some other private as well as communal enclosures. 相似文献
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