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951.
Early development of the Antarctic sea urchin Sterechinus neumayeri was examined under two differ-ent culture regimes: one to simulate development near-bottom (“demersal development”) and the
other to simulate the development of embryos in the water column (“pelagic development”). When embryos of both treatments
reached the hatching blastula stage at 5 d post-fertilization (−1.5 °C), the blastulae that had undergone demersal development
evidenced significant differences (by ANOVA or suitable non-parametric comparison) in the following: a thicker blastoderm
layer (12%, P < 0.001), higher ash-free dry weights (19%, P < 0.01), lower mass-specific respiration rates (50%, P < 0.001), higher incorporation rates of 35S-methionine into protein (23%, P < 0.003), and a differential pattern of protein synthesis. When embryos developed demersally, they remained in the jelly-coat
material released with the eggs at spawning. Quantitative isolation of this jelly-coat material in S. neumayeri demonstrated that it contained a significant amount of organic matter, 115 ng ash-free dry mass per egg, equivalent to 17%
of the egg's initial organic mass. Uptake of external nutrients during embryogenesis may be a significant component of the
physiological energetics of this polar invertebrate by allowing the utilization of jelly-coat material released by a female
during spawning.
Received: 21 April 1999 / Accepted: 5 June 2000 相似文献
952.
The predation impact of the two chaetognaths Eukrohnia hamata and Sagitta gazellae on mesozooplankton standing stock were investigated in three depth layers during two 24 h stations occupied in the vicinity
of Marion Island in late austral summer (April/May) 1986. The zooplankton community at both stations was dominated by small
copepods (Oithona spp., Microcalanus spp.), which accounted for >95% of total zooplankton abundance. Chaetognaths comprised <2% of total zooplankton abundance.
E. hamata constituted >95% of the total chaetognath stock. The general trend in both species was decreasing abundance with increasing
depth, which appeared to be correlated to the distribution of copepods (r
2 = 0.45; P <0.05). Gut-content analysis showed that copepods (mainly Oithona spp., Calanus spp. and Rhincalanus gigas) and ostracods were the main prey of both species, accounting for 87 and 61% of the total number of prey in E. hamata and S.␣gazellae stomachs, respectively. In the guts of S.␣gazellae, pteropods (Limacina spp.) and chaetognaths were also well represented. The mean number of prey items (NPC) for E. hamata ranged from 0.02 to 0.06 prey individual−1 which corresponds to an individual feeding rate (Fr) of between 0.05 and 0.12 prey d−1. For S.␣gazellae, the NPC values were higher, varying between 0.04␣and 0.20 prey individual−1, or between 0.15 and 0.76 prey d−1. The daily predation impact of the two chaetognaths was estimated at between 0.3 and 1.2% of the copepod standing stock or
between 7 and 16% of the daily copepod production. Predation by S. gazellae on chaetognaths accounted for up to 1.6% of the chaetognath standing stock per day.
Received: 26 November 1996 / Accepted: 31 October 1997 相似文献
953.
The relationship between sperm characteristics and reproductive success was examined in male herring, Clupea harengus L. Males were categorised as being first-time or repeat spawners on the basis of their age; they were also grouped according
to whether their sperm were immediately active and exhibited forward motion on contact with seawater (FM) or had little or
only vibratory motion (VM). Unlike the Pacific herring C. pallasii Valencienes, Atlantic herring sperm is usually motile on contact with seawater. The age, weight and gonadosomatic index (testes
mass as a percentage of somatic mass = GSI) were measured and used as characteristics for individual fish. Sperm traits measured
were (1) adenosine triphosphate (ATP) concentration, (2) sperm count, (3) duration of sperm motility. Reproductive success
for each male was estimated from the fertilisation rate and from the length of larvae at hatching. Fertilisation rates for
all fish were generally >80%. The ATP concentration of non-activated spermatozoa was negatively correlated with fertilisation
rate. Among repeat spawners, fish with higher GSIs produced larvae that were larger at hatching. Although VM sperm fertilised
eggs at rates equivalent to fertilisation by FM sperm, the larvae produced by VM sperm were significantly smaller at hatching.
Larval length tended to increase in parallel with the duration of sperm motility, but the relationship was not significant
in these tests. The results did not indicate any age or size pattern to spawning readiness in male herring. Sperm that are
not yet ready to be shed are not fully motile on contact with seawater, but are still capable of fertilising eggs that hatch
successfully. There is likely to be a progression of males which come into spawning readiness within a spawning shoal; therefore
it is possible that paternal influences would result in a progressive decrease in larval size over the spawning period in
winter-spawning Celtic Sea herring.
Received: 22 November 1997 / Accepted: 8 June 1998 相似文献
954.
The sibling species, Pseudocalanus moultoni (Frost, 1989) and P. newmani (Frost, 1989), occur sympatrically on Georges Bank. Taxonomic discrimination of the species relies on subtle morphological
characteristics, making routine identification of the species very difficult. DNA sequence variation of two mitochondrial
genes, 16S rRNA and cytochrome oxidase I (COI), reliably discriminated P. moultoni and P.␣newmani. Levels of DNA sequence variation for both genes were consistent with those between species of calanoid copepods. A molecular
systematic protocol (based on allele-specific PCR amplification) was designed from the COI sequences and used to discriminate
females of the two species. The distributions and relative abundances of the two species were mapped for April 1996 based
on samples of 15 to 30 females from 12 collections across Georges Bank. The results of this study indicated that P. moultoni females predominated along the northern flank of Georges Bank, while P. newmani females were common on the southern flank, deeper than the 60-m isobath.
Received: 16 May 1997 / Accepted: 26 April 1998 相似文献
955.
Common shrimp, Crangon crangon (L.), were exposed to inorganic arsenic (arsenate), trimethylarsine oxide, or arsenobetaine in sea water (100 μg As l−1) or in food (1 mg As g−1 wet wt) for up to 24 d, followed by 16 d depuration in clean sea water with undosed food, in order to determine the efficiency
of uptake and retention of the compounds. Accumulation of arsenic in the tail muscle, gills, midgut gland, exoskeleton, and
remaining tissues was found to depend on the chemical form of the arsenic and the route of exposure. No arsenic was accumulated
by C. crangon exposed to arsenate or trimethylarsine oxide in sea water. Shrimps exposed to waterborne arsenobetaine initially accumulated
a small amount of arsenic in their tail muscle and gills. After 16 d, C. crangon fed arsenate, trimethylarsine oxide, or arsenobetaine had accumulated arsenic in their tail muscle to levels ∼2-, 2-, or
40-times, respectively, that of the control group. A roughly linear rate of accumulation was shown by shrimps fed trimethylarsine
oxide or arsenobetaine, but C. crangon fed arsenate accumulated arsenic for 16 d, then lost arsenic such that their concentration on Day 24 was not significantly
different from that of the control group. Patterns of arsenic accumulation in the gills of shrimps fed the compounds were
similar to those seen in the tail muscle. On a whole animal basis, C. crangon retained ∼1.2% of the arsenate, 1.6% of the trimethylarsine oxide, and 42% of the arsenobetaine consumed over the first 16 d
of exposure, with roughly half present in the tail muscle in each case. Data obtained support the view that the direct uptake
of arsenobetaine from sea water does not make a significant contribution to the relatively high concentrations of this compound
in marine crustaceans, and that food is the primary source. Naturally occurring arsenic compounds in C. crangon and possible transformations of the administered arsenic compounds were examined by high performance liquid chromatography
using an inductively coupled plasma mass spectrometer as the arsenic-specific detector. Control C. crangon contained arsenobetaine as the major arsenic compound (>95% of total arsenic); tetramethylarsonium ion (0.7%) and an unknown
arsenic compound (1.7%) were also present as minor constituents. Shrimp ingesting arsenobetaine accumulated it unchanged.
Shrimp ingesting arsenate did not form methylated arsenic compounds; they appeared to contain their accumulated arsenic as
unchanged arsenate only, although the possibility that some of the arsenic was reduced to arsenite could not be excluded.
C. crangon ingesting trimethylarsine oxide biotransformed the compound predominantly to dimethylarsinate.
Received: 9 October 1997 / Accepted: 11 February 1998 相似文献
956.
Recovery of benthic communities at Macquarie Island (sub-Antarctic) following a small oil spill 总被引:5,自引:0,他引:5
Intertidal communities at Macquarie Island (sub-Antarctic) were re-evaluated seven years after a small oil spill resulting
from the grounding of the “Nella Dan”, and six years after the first assessment of biological impact. Sampling was conducted
to evaluate community structure in three zones of the exposed rocky shore (upper red, kelp, and lower red) and in samples
of Durvillaea antarctica holdfasts. There were no significant differences between the community structure in oiled and control locations in any of
the three shore zones, but holdfast macrofaunal communities at oiled sites still showed evidence of impact. Holdfast community-structure
in samples from heavily oiled sites showed moderate levels of recovery, with increased abundances of species which were considered
sensitive to the oiling in the first post-impact studies and decreased abundances of opportunistic polychaete and oligochaete
worms. In contrast, samples collected from the moderately oiled location at Secluded Bay showed little evidence of recovery.
Holdfasts at this site were filled with sediment containing traces of diesel oil, and the macrofaunal community was dominated
by opportunistic worms. This study clearly indicates that even small incidents of anthropogenic perturbation can have long-lasting
consequences for marine communities at Macquarie Island.
Received: 23 July 1997 / Accepted: 5 February 1998 相似文献
957.
Reproduction in the sea urchin Centrostephanus rodgersii was examined in two types of habitats (“barrens”, i.e. habitats characterised by the high crustose coralline algal cover
typical of urchin-barren grounds, and by the absence of macroalgae; and “fringe”, i.e. habitats characterised by a high macroalgal
biomass and few C. rodgersii) at four locations in New South Wales. The four locations: the Solitary Islands, Sydney, Ulladulla and Eden, span the distribution
of C.␣rodgersii from the subtropics at its northern limit to temperate waters near its southern limit. Histology and estimates of gonad retrieval
rate (GRR) from January 1994 to October 1995 indicated that reproduction was synchronous at all locations. An increase in
the tempo of gametogenesis in May and onset of spawning in June at all locations is consistent with entrainment in response
to exogenous factors. Over the range studied, C. rodgersii experienced relatively similar daylength cycles and contrasting sea-temperature cycles. Short days and lunar conditions coinciding
with the solstice appear likely proximate cues for the onset of spawning. The major difference in reproduction among locations
was in the duration of spawning. In the southern parts of its range breeding occurred over a 5 to 6 mo period, whereas at
the Solitary Islands it lasted ≃1 mo. At most locations the GRRs were significantly higher in the fringe habitat than in the
barrens habitat. The lower reproductive output of urchins in the barrens habitat was attributed to the food-poor conditions
typical of this habitat. The developing fishery for C. rodgersii is likely to be most effective from March to early May. Urchins from barrens areas may not provide sufficient yield to warrant
harvesting.
Received: 29 October 1997 / Accepted: 18 May 1998 相似文献
958.
The effects of blooms of the cyanobacterium Trichodesmium spp. on penaeid prawn larvae were examined using in situ and laboratory rearing experiments and plankton surveys in Albatross
Bay, Gulf of Carpentaria, Australia. The in situ experiments demonstrated that, during a bloom of Trichodesmium spp., larvae of the prawn Penaeus merguiensis did not develop beyond the first protozoea stage, and survival was low compared with times when diatoms were dominant in
the same study area. Laboratory experiments confirmed the in situ results. None of the prawn larvae fed Trichodesmium sp. in laboratory experiments developed beyond the first protozoeal stage. In contrast, 94% of prawn larvae fed the green
flagellate Tetraselmis suecica successfully developed to the second protozoea stage. Electron microscopy of larvae gut-contents revealed that Trichodesmium spp. were ingested by larvae but were of no nutritional value, resulting in starvation. A 7 yr plankton survey, from 1985
to 1992, showed that minimum abundance of prawn larvae occurs during the annual summer blooms of Trichodesmium spp. and that maximum abundance of prawn larvae generally occurs just after the bloom. There was a negative correlation between
the abundance of larvae and the abundance of Trichodesmium at individual sites, one offshore and one inshore, indicating that the blooms affect the survival of larvae. We conclude that
variations in both timing and magnitude of Trichodesmium blooms are important determinants of prawn larvae abundance in Albatross Bay.
Received: 28 April 1997 / Accepted: 2 April 1998 相似文献
959.
Previously published mtDNA cytochrome b sequences for Xyrichtys novacula (Linnaeus) and two morphologically distinct types of Xyrichtys larvae (Pisces: Labridae) included human cytochrome b sequence, presumably due to sample contamination and/or poor preservation of sample DNA. Those sequences had been used to
identify the two types of Xyrichtys larvae as X. novacula, but owing to the contamination, the identifications are invalid. Fresh specimens were collected: X.␣martinicensis (Cuvier and Valenciennes), X. novacula, X. splendens (Castelnau), and Xyrichtys sp. larvae of each of the two morphotypes (dorsal-forward eye and ventral-forward eye). The cytochrome b fragment was amplified from each specimen using the polymerase chain reaction. Comparison of the sequences with human cytochrome b sequence confirmed that the new sequences were not contaminated. The interspecific differences in Xyrichtys sequences were less than previously reported, but still greater than observed in many fish genera. Distance and parsimony
analyses indicated that X. novacula and X. martinicensis were more closely related to each other than to X. splendens. This conclusion differs from our previous conclusion that X. martinicensis and X. splendens were more closely related to each other than to X. novacula. Distance and parsimony analyses also demonstrated that both larval morphotypes were X. novacula and, thus, our previous conclusions regarding larval ecology and oceanographic transport remain unchanged.
Received: 12 March 1997 / Accepted: 24 October 1997 相似文献
960.
Effect of female molt stage and sex ratio on courtship behavior of the blue crab Callinectes sapidus
In many species, males and females actively participate in courtship, and the outcome of pre-mating interactions influences
the mating success of both sexes. Female blue crabs, Callinectes sapidus, mate soon after their final molt to maturity; thus female molt stage dictates the timing of mating. In a field experiment,
we manipulated female molt stage and sex ratio to test their effects on the courtship behavior of both sexes, if female behavior
influences the behavior and pairing success of males, and if male courtship influences male pairing-success. Early-molt-stage
females avoided males during courtship, whereas late-molt-stage females sought out males. As a result, males had to pursue
and capture early-molt-stage females whereas males displayed to late-molt-stage females and more easily physically controlled
them. Males sometimes abandoned late-molt-stage females, but this occurred more often when females were abundant. The rate
at which females avoided males was positively correlated with that of males abandoning females, and males that were unsuccessful
at pairing met with higher rates of female resistance than successful males, suggesting that female behavior influences male
pairing-success. Unlike unsuccessful males, successful males more often made the transition between display and maintaining
physical control of the female. At high male sex ratios, males initiated courtship more readily; thus both sexual competition
and female behavior influence male courtship in this species.
Received: 7 July 1996 / Accepted: 10 January 1998 相似文献