Analysis of macroinvertebrate assemblages in relation to environmental gradients among lotic habitats of California's Central Valley

We assessed relationships between environmental characteristicsand macroinvertebrate assemblages in lotic habitats of California's Central Valley with community metric and multivariate statistical approaches. Using canonical ordinationanalyses, we contrasted results when assemblage structure was assessed with macroinvertebrate metrics, as suggested for use inindices of biotic integrity, or with genera abundances. Our objectives were to identify metrics or taxa diagnostic of loticenvironmental stressors and compare the capacity of theseapproaches to detect stressors in order to suggest how they mightbe used to diagnose stressors. For macroinvertebrate metrics,redundancy analysis (RDA) extracted three axes correlated withchannel morphology and substrates. For genera abundances, canonical correspondence analysis (CCA) extracted two axescorrelated with soluble salts and with channel morphology andsubstrates but did not separate these gradients onto differentaxes. Cluster analyses identified five RDA and five CCA sitegroups, which exhibited differences for environmental variables,metrics, or genera abundances, and agreement between the analysesin partitioning of sites was greater than if sites were partitioned randomly. These approaches differ in their abilityto detect environmental stressors, because they measure differentaspects of assemblages and would be complementary in design ofnew metrics diagnostic of stressors.     

Source identification and assessment of sediment contamination of trace metals in Kogarah Bay,NSW, Australia

The distribution of trace metals (spatial and temporal) and sedimentary fractions were investigated to identify the concentrations and sources of trace metals within Kogarah Bay, NSW, Australia. A total of 59 surface sediments and six subsurface samples from core of the sediment were collected. The contamination factor and pollution load index indices used to evaluate environmental effects of trace metals. The study area was found to be uncontaminated with Cr and Ni, moderately contaminated with As and considerably contaminated with Cu, Zn and Pb. The concentrations of Cr and Ni were below both effect range low and effect range median, while As, Cu, Zn and Pb were slightly above effect range low. The highest concentrations of these trace metals such as Cu, Zn and Pb were found in the north, northwest and southeast of the bay, close to discharge points, stormwater outlets and around boatyards and watercrafts. The spatial distributions of metals were strongly related to muddy particles and organic matter. The temporal sediments of metals declined with increased sediment depth, which reflects accumulation of trace metals since European settlement in this area. Furthermore, the source of the trace metals was found to be stormwater outlets, gasoline fumes, boatyards and other human activities.     

Biofilm cue for larval settlement in Hydroides elegans (Polychaeta): is contact necessary?

Larvae of many sessile marine invertebrates settle in response to surface microbial communities (biofilms), but the effects of soluble compounds from biofilms in affecting larval behavior prior to settlement, attachment, and metamorphosis have been little studied. This question was addressed by videotaping the behavior of competent larvae of the serpulid, Hydroides elegans, above settlement-inducing biofilms. Adult worms were collected in Pearl Harbor, Hawaii, USA in November 2012 and spawned almost immediately. Six-day old larvae were placed in five replicated treatments in small cups: (1) with a natural biofilm; (2) with a natural biofilm on an 8-µm screen, 1 mm above the bottom of a clean cup; (3) with a natural biofilm beneath a clean screen; (4) in a clean cup; and (5) in a clean cup with a clean screen. Using the videotapes, larval swimming speeds and trajectories were quantified within 5 min of the larvae being placed in a treatment. Only larvae that touched a biofilm, i.e., in treatments (1) and (2), slowed their swimming speed and increased the amount of time spent crawling rather than swimming. This shows that under these conditions, any soluble cues emanating from a biofilm do not affect settlement behavior. Furthermore, after 24 h close to 100 % of larva in the two accessible biofilm treatments had metamorphosed and <15 % in treatments that included a biofilm under a clean screen and no biofilm at all, strongly suggesting that soluble cues for settlement were not produced by the biofilms over the longer time period.     

Prenatal detection of monosomy 21 mosaicism

We report a case of chromosomal mosaicism for monosomy 21 revealed in amniotic fluid cell culture. Ultrasound examination at 19 weeks' gestation showed in utero growth retardation and a complex cardiac malformation. A repeated amniocentesis confirmed the presence of monosomy 21 mosaicism. In view of the sonographically detected fetal abnormalities, termination of pregnancy was elected.     

Cytogenetic analysis of 1375 amniotic fluid specimens from pregnancies with gestational age less than 14 weeks

Our laboratory has received 1375 early amniotic fluid (EA) specimens during the past 5-year period for cytogenetics analysis. The gestational ages of the EA specimens were less than 14 weeks as estimated by ultrasound. The average volume of specimen received was 16 ml. Specimens were typically received in two collection tubes and cultured in Chang A and in supplemented MEM media using the in situ technique. Of the 1375 EA specimens received, 1356 were successfully cultured and yielded results. Abnormal results were found in 67 (4.9 per cent) of the cases. Nineteen specimens (1.4 per cent) failed to yield a result. The mean turn-around time (TAT) for all EA specimens was 8.28 days. In 1991, the average TAT for the EA specimens was 8.00 days compared with a TAT of 6.59 days for all specimens received over 14 weeks gestational age. The number of EA specimens received has increased from 1.5 per month in 1986 to 57 per month in 1991. In summary, our experience with EA specimens for cytogenetic analysis has demonstrated that the success rate is 98.6 per cent and that an increasing number of obstetricians are performing early amniocentesis as they seek to provide their patients with earlier results and an alternative to chorionic villus sampling.     

High-valent iron-based oxidants to treat perfluorooctanesulfonate and perfluorooctanoic acid in water

Perfluoroalkyl and polyfluoroalkyl substances are occurring in consumer and industrial products. They have been found globally in the aquatic environment including drinking water sources and treated wastewater effluents, which has raised concern of potential human health effects because these substances may be bioaccumulative and extremely persistent. The saturated carbon–fluorine bonds of the substances make them resistant to degradation by physical, chemical, and biological processes. There is therefore a need for advanced remediation methods. Iron-based methods involving high-valent compounds are appealing to degrade these substances due to their high oxidation potentials and capability to generate environmentally friendly by-products. This article presents for the first time the oxidation ability of tetraoxy anions of iron(V) (Fe^VO₄ ^3?, Fe(V)), and iron(IV) (Fe^IVO₄ ^4?, Fe(IV)), commonly called ferrates, in neutral and alkaline solutions. Solid compounds of Fe(V) (K₃FeO₄) and Fe(IV) (Na₄FeO₄) were added directly into buffered solution containing perfluorooctansulfonate and perfluorooctanoic acid at pH 7.0 and 9.0, and mixed solutions were subjected to analysis for remaining fluoro compounds after 5 days. The analysis was performed by liquid chromatography–mass spectrometry/mass spectrometry technique. Fe(IV) showed the highest ability to oxidize the studied contaminants; the maximum removals were 34 % for perfluorooctansulfonate and 23 % for perfluorooctanoic acid. Both Fe(V) and Fe(IV) had slightly higher tendency to oxidize contaminants at alkaline pH than at neutral pH. Results were described by invoking reactions involved in oxidation of perfluorooctansulfonate and perfluorooctanoic acid by ferrates in aqueous solution. The results demonstrated potentials of Fe(V) and Fe(IV) to degrade perfluoroalkyl substances in contaminated water.     

Change point analysis of phosphorus trends in the Illinois River (Oklahoma) demonstrates the effects of watershed management

Detecting water quality improvements following watershed management changes is complicated by flow-dependent concentrations and nonlinear or threshold responses that are difficult to detect with traditional statistical techniques. In this study, we evaluated the long-term trends (1997-2009) in total P (TP) concentrations in the Illinois River of Oklahoma, and some of its major tributaries, using flow-adjusted TP concentrations and regression tree analysis to identify specific calendar dates in which change points in P trends may have occurred. Phosphorus concentrations at all locations were strongly correlated with stream flow. Flow-adjusted TP concentrations increased at all study locations in the late 1990s, but this trend was related to a change in monitoring practices where storm flow samples were specifically targeted after 1998. Flow-adjusted TP concentrations decreased in the two Illinois River sites after 2003. This change coincided with a significant decrease in effluent TP concentrations originating with one of the largest municipal wastewater treatment facilities in the basin. Conversely, flow-adjusted TP concentrations in one tributary increased, but this stream received treated effluent from a wastewater facility where effluent TP did not decrease significantly over the study period. Results of this study demonstrate how long-term trends in stream TP concentrations are difficult to quantify without consistent long-term monitoring strategies and how flow adjustment is likely mandatory for examining these trends. Furthermore, the study demonstrates how detecting changes in long-term water quality data sets requires statistical methods capable of identifying change point and nonlinear responses.     

Biophysical‐Regulatory Classification and Profiling of Streams Across Management Units and Ecoregions1

Caruso, Brian S. and Joshua Haynes, 2011. Biophysical‐Regulatory Classification and Profiling of Streams Across Management Units and Ecoregions. Journal of the American Water Resources Association (JAWRA) 00(0):1‐22. DOI: 10.1111/j.1752‐1688.2010.00522.x Abstract: Aquatic resources management in the United States (U.S.) under Clean Water Act Section 404 has become more complex after recent Supreme Court decisions and U.S. Army Corps of Engineers and Environmental Protection Agency (USEPA) guidance. Many intermittent/ephemeral and headwater streams may not be jurisdictional if they lack a significant nexus with navigable waters. Streams in semiarid USEPA Region 8 were classified based on hydrologic permanence and stream order using National Hydrography Dataset (NHD) Plus and GIS to provide information across broad spatial scales to aid with jurisdictional determinations (JDs). Four classes were developed for profiling across management units and ecoregions. Based on medium‐resolution NHDPlus data, intermittent streams comprise >¾, and first order streams constitute >½ of the total stream length in Region 8. Mountain states and ecoregions have the largest percentage of perennial first order streams, whereas the Dakotas, plains, and desert ecoregions have the greatest percentages of intermittent first order and intermittent higher order streams. In the Upper Colorado River Basin, >50% of reaches are intermittent first order, and 9% are perennial first order. NHDPlus data can significantly underestimate the length of headwater and intermittent streams, but can still be a valuable tool to help develop stream classes and for regional JD planning and analysis. Refinement of the stream classes using high resolution NHD data and other key catchment parameters can improve their utility for JDs.     

Estimating trend precision and power to detect trends across grouped count data

Ecologists commonly use grouped or clustered count data to estimate temporal trends in counts, abundance indices, or abundance. For example, the U.S. Breeding Bird Survey data represent multiple counts of birds from within each of multiple, spatially defined routes. Despite a reliance on grouped counts, analytical methods for prospectively estimating precision of trend estimates or statistical power to detect trends that explicitly acknowledge the characteristics of grouped count data are undescribed. These characteristics include the fact that the sampling variance is an increasing function of the mean, and that sampling and group-level variance estimates are generally estimated on different scales (the sampling and log scales, respectively). We address these issues for repeated sampling of a single population using an analytical approach that has the flavor of a generalized linear mixed model, specifically that of a negative binomial-distributed count variable with random group effects. The count mean, including grand intercept, trend, and random group effects, is modeled linearly on the log scale, while sampling variance of the mean is estimated on the log scale via the delta method. Results compared favorably with those derived using Monte Carlo simulations. For example, at trend = 5% per temporal unit, differences in standard errors and in power were modest relative to those estimated by simulation (< or = /11/% and < or = /16/%, respectively), with relative differences among power estimates decreasing to < or = /7/% when power estimated by simulations was > or = 0.50. Similar findings were obtained using data from nine surveys of fingernail clams in the Mississippi River. The proposed method is suggested (1) where simulations are not practical and relative precision or power is desired, or (2) when multiple precision or power calculations are required and where the accuracy of a fraction of those calculations will be confirmed using simulations.     

Windows of opportunity: historical and ecological controls on Berberis thunbergii invasions

Attempts to determine characteristics that render habitats invasible to nonnative species have met with limited success. This may be because most studies focus on modern habitat conditions and do not consider invasibility in the context of a historically dynamic landscape in which both the abundance of a species and the invasibility of a site may change. We surveyed 159 currently forested sites for the occurrence and abundance of Berberis thunbergii (Japanese barberry), an invasive, nonnative shrub in forests of the northeastern United States, relative to modern environmental conditions, contemporary logging activity, and two periods of historical land use. Berberis thunbergii occurred more frequently and was more abundant in post-agricultural forests than in continuously wooded sites. This relationship was stronger for agricultural sites that were abandoned and reforested after B. thunbergii was introduced to the region than for sites that reforested prior to B. thunbergii introduction. In contrast, recent forest harvesting did not influence the occurrence or abundance of B. thunbergii. Modern soil fertility explained a significant portion of the variation in B. thunbergii occurrence, whereas site history considerably improved predictions of population density and helped evaluate potential invasion mechanisms. While land-use history covaries with soil fertility and distance to putative seed sources, the strong relationship between modern abundance patterns and historical agriculture suggests that B. thunbergii colonized recently abandoned agricultural lands in the early 20th century and then persisted and spread locally during subsequent reforestation. Our results indicate that interpretations of both native community composition and modern plant invasions must consider the importance of historical landscape changes and the timing of species introduction along with current environmental conditions.