Fatty acid composition of marine macroalgae from the Black Sea and Dardanelles

The fatty acid (FA) composition was determined in 14 species of marine macroalgae belonging to the Chlorophyta, Phaeophyta and Rhodophyta, which were collected from ?ile in the Black Sea and Kepez in the Dardanelles. Generally, polyunsaturated FAs and monounsaturated FAs were major components (50–77%). Total saturated FAs ranged from 22% to 50%, with 16?:?0 as the most abundant saturate (32–38%). Two samples of Cystoseira barbata collected from a different station had some differences from each other in their contents of 18?:?2n-6 and 18?:?3n-3 and in the 18?:?2n-6/20?:?4n-6 ratios. Green algal species had a significantly higher proportion of unsaturated FAs and a significantly lower proportion of saturated FAs than the red and brown algae. The amount of n-3 FAs was significantly higher in Ulva rigita, Chaetomorpha linum, Enteromorpha linza and Gracilaria verrucosa (8.88, 6.44, 5.31 and 5.24, respectively).     

Carbofuran-induced effects on acetylcholinesterase (AChE) in erythrocytes and liver of Cyprinus carpio

A study was conducted to determine the effects of long-term exposure to carbofuran on erythrocyte and liver acetylcholinesterase (AChE) activity in Cyprinus carpio, and assess which tissue was more sensitive to pesticide exposure examining this enzyme. Fish were allowed to accilimatize in toxicant-free water for 24 days after 60-day exposure. AChE activity was determined with a spectrophotometer using acetylthiocholine iodide as a substrate in erythrocytes and liver. Enzymic activity of erythrocytes and liver decreased over time. A higher degree of enzyme inhibition was observed in erythrocyte compared to liver. The degree of enzyme inhibition was positively correlated with exposure time. When exposed fish were transferred to clean water, recovery was greater in erythrocytes than liver. Erythrocyte AChE activity recovered after 18 days, while it required 21 days in liver. The findings of this study indicate that erythrocyte AChE is more sensitive to carbofuran than liver. The greater sensitivity of erythrocyte AChE suggests that it may be more useful as a biomarker for monitoring status of pollution in freshwater bodies.     

Physicochemical properties of non-living water hyacinth (Eichhornia crassipes) and lesser duckweed (Lemna minor) and their influence on the As(V) adsorption processes

Eichhornia crassipes (Ec) and Lemna minor (Lm) are aquatic plants. They are considered as weeds of the water and approach being a scourge in many parts of the world, choking waterways and hindering transport upon them. At the same time they are known to readily remove heavy metal ions from water. This paper considers the use of non-living plants as novel and inexpensive biosorbent for the removal of As(V) from watersheds. In the first place they were conditioned and characterised to determine their physicochemical and surface properties and in the second place their adsorption properties for As(V) from aqueous solution were evaluated considering the toxicity of this metalloid in the environment. It describes the methodology to prepare the non-living biomasses; the physicochemical characterisation by SEM, XRD, FTIR, TGA analyses and surface characterisation of Ec and Lm by specific surface, hydration kinetic, point of zero charge determination by mass titration, active site density and XPS analysis are described. Both studied biomasses were found to be potential bio-sorbents for arsenic ions from aqueous solution. According to their efficiency to remove arsenic, they can be used in a very low cost metalloid ions removal system.     

The impact of enhanced ammonium sulphate inputs exceeding critical load on calluna vulgaris /peat soil microcosms

Ammonium sulphate at six concentrations in simulated precipitation has been applied weekly over two years to Calluna vulgaris growing in peat soil. The nitrogen deposition treatments were chosen to embrace and exceed critical load. The growth and composition of the Calluna and the changes over time in the chemistry of the peat soil and its soil solution were monitored. In spite of significant increases in foliar nitrogen concentration in new shoots, especially in the first year, growth did not increase significantly in response to nitrogen treatment. Several factors could be contributing to the lack of significant growth response. (1) Increasing ammonium input significantly acidified the soil solution, which could adversely effect growth directly. (2) Foliar calcium concentration was reduced significantly in both years by the ammonium sulphate treatments, and more calcium was undoubtedly lost from the rooting zone at higher nitrogen inputs. (3) Foliar phosphate declined significantly between the first and second year, so lack of growth response might also reflect a phosphorus limitation. There was a distinctly visible darkening of the leaves in response to increasing ammonium applications, especially for the first year's growth, and the chlorophyll a and b concentrations in leaves from new growth at the three highest nitrogen treatments were significantly (at P <0.05) higher than those for the control. The pigment concentrations fell markedly by the end of the second season, and treatment effects were much less consistent. It is suggested that pigment analysis therefore probably has little diagnostic value for assessing damage from pollutant nitrogen effects.     

Natal dispersal in two mice species with contrasting social systems

We compared the natal dispersal behaviour of two mice species under laboratory conditions. Natal dispersal is a movement of an animal from its birthplace to its breeding area. This behaviour is known to be influenced by the mating system. In polygamous species, males are more likely to disperse, while in most of the monogamous species, both sexes disperse. Our subjects, the house mouse (Mus musculus) and the mound-building mouse (Mus spicilegus) are two sympatric species of the genus Mus. Both are native in Hungary, but they differ in their habitat type mating system and overwintering strategy. The house mouse is a polygynous species and adapted to human environment, known for mature and reproduce early. On the contrary, the mound-building mice are monogamous, and they inhabit extensively used agricultural fields, where they spend the unfavourable winter period in nest chambers under mounds, which they construct from soil and plant material. Successful overwintering for this species demands delayed maturity and reduced dispersion during the winter. Our results showed that the natal dispersal of these two species differ; both sexes of the mound-building mice dispersed later than the house mice, where a difference between sexes also occurs; house mice males dispersed earlier than females. The mound-building mice showed no sexual dimorphism in this behaviour.     

Status,source identification,and health risks of potentially toxic element concentrations in road dust in a medium-sized city in a developing country

This study aims to determine the status of potentially toxic element concentrations of road dust in a medium-sized city (Rawang, Malaysia). This study adopts source identification via enrichment factor, Pearson correlation analysis, and Fourier spectral analysis to identify sources of potentially toxic element concentrations in road dust in Rawang City, Malaysia. Health risk assessment was conducted to determine potential health risks (carcinogenic and non-carcinogenic risks) among adults and children via multiple pathways (i.e., ingestion, dermal contact, and inhalation). Mean of potentially toxic element concentrations were found in the order of Pb > Zn > Cr(IV) > Cu > Ni > Cd > As > Co. Source identification revealed that Cu, Cd, Pb, Zn, Ni, and Cr(IV) are associated with anthropogenic sources in industrial and highly populated areas in northern and southern Rawang, cement factories in southern Rawang, as well as the rapid development and population growth in northwestern Rawang, which have resulted in high traffic congestion. Cobalt, Fe, and As are related to geological background and lithologies in Rawang. Pathway orders for both carcinogenic and non-carcinogenic risks are ingestion, dermal contact, and inhalation, involving adults and children. Non-carcinogenic health risks in adults were attributed to Cr(IV), Pb, and Cd, whereas Cu, Cd, Cr(IV), Pb, and Zn were found to have non-carcinogenic health risks for children. Cd, Cr(IV), Pb, and As may induce carcinogenic risks in adults and children, and the total lifetime cancer risk values exceeded incremental lifetime.     

Research progress in microbial cytochrome P450 and xenobiotic metabolism北大核心CSCD

Cytochrome P450 (CYP450s) is a type of heme-mercaptide protein superfamily, which is distributed widely in animals, plants, and microorganisms. CYP450s can oxidize and degrade many exogenous compounds such as drugs, herbicides, pesticides, some persistent organic pollutants, and so on. Based on recent researches, this paper reviews the nomenclature, classification, structure, and catalytic mechanism of P450 enzymes, and summarizes the research progresses in the metabolism and biodegradation of xenobiotics using P450 enzymes from microorganisms. The nomenclature and classification of the P450 gene superfamily mainly rely on the similarities of amino acid sequences. Although the structures of P450 are conserved, their recognition sites towards to the substrates are variable. This is also the structural basis for the catalytic diversities of P450 enzymes. Few P450 enzymes from bacteria and fungi can metabolize and degrade xenobiotics such as polycyclic aromatic hydrocarbons, herbicides, and so on. However, these P450 enzymes are less likely be used in practical applications because of their low catalytic activities. In the future, more P450 enzymes with high degradable efficiencies towards xenobiotics are needed to be obtained using multiple omics tools or modifying the existing P450 enzymes, to achieve the bioremediation of the environment. © 2018 Science Press. All rights reserved.     

The interaction of heat shock proteins and some components of plant viruses北大核心CSCD

Considerable research has indicated that heat shock proteins (Hsp), as molecular chaperones, carry out many biological activities of plant viruses by folding, transporting, translocating, assembling, or degrading client proteins. It is fundamental to develop resistant plant varieties and novel anti-viral agents by determining the interaction mechanisms between plant viruses and hosts. In this study, we first reviewed the classification, gene and protein structure, and biological significances. We then analyzed the assembling mechanism of Hsp70 or Hsp90, plant host cofactors, and RNA-dependent RNA polymerases in a viral replicase complex, and the mechanism of interaction and subcellular localization between Hsp70 and some plant virus components. We highlighted the mechanism of interaction and movement between Hsp70 and some plant virus components and the effect of Hsp expression of plant hosts or viruses. The results indicated where the mechanism occurred, the participating factors, energy supply, and material conversion between Hsps and the plant virus components for the course of the intracellular movement, local movement between cells, and long-distance movement, and showed the Hsp type specificity and the law of dynamic Hsp expression in plant hosts infected by viruses. The studies mainly focused on the two Hsp factors and the plant viral components, indicating limited coordination mechanisms among many nucleic acids, proteins, and polysaccharides in macromolecular protein complexes (MRC). Future research should analyze the translocation mechanism between client proteins and Hsps, the coordination mechanism between Hsps and MRC components, and the relation between MRC and the plant tissue structure. © 2018 Science Press. All rights reserved.     

Functionally relevant microorganisms to enhanced biological phosphorus removal performance at full-scale wastewater treatment plants in the United States

The abundance and relevance ofAccumulibacter phosphatis (presumed to be polyphosphate-accumulating organisms [PAOs]), Competibacter phosphatis (presumed to be glycogen-accumulating organisms [GAOs]), and tetrad-forming organisms (TFOs) to phosphorus removal performance at six full-scale enhanced biological phosphorus removal (EBPR) wastewater treatment plants were investigated. Coexistence of various levels of candidate PAOs and GAOs were found at these facilities. Accumulibacter were found to be 5 to 20% of the total bacterial population, and Competibacter were 0 to 20% of the total bacteria population. The TFO abundance varied from nondetectable to dominant. Anaerobic phosphorus (P) release to acetate uptake ratios (P(rel)/HAc(up)) obtained from bench tests were correlated positively with the abundance ratio of Accumulibacter/(Competibacter +TFOs) and negatively with the abundance of (Competibacter +TFOs) for all plants except one, suggesting the relevance of these candidate organisms to EBPR processes. However, effluent phosphorus concentration, amount of phosphorus removed, and process stability in an EBPR system were not directly related to high PAO abundance or mutually exclusive with a high GAO fraction. The plant that had the lowest average effluent phosphorus and highest stability rating had the lowest P(rel)/HAc(up) and the most TFOs. Evaluation of full-scale EBPR performance data indicated that low effluent phosphorus concentration and high process stability are positively correlated with the influent readily biodegradable chemical oxygen demand-to-phosphorus ratio. A system-level carbon-distribution-based conceptual model is proposed for capturing the dynamic competition between PAOs and GAOs and their effect on an EBPR process, and the results from this study seem to support the model hypothesis.     

28-homobrassinolide protects chickpea (Cicer arietinum) from cadmium toxicity by stimulating antioxidants

In the present experiment the seeds of Cicer arietinum (L.) cv. Uday were inoculated with specific Rhizobium grown in sandy loam soil and were allowed to grow for 15 days. At this stage, the seedlings were supplied with 0, 50, 100 or 150 microM of cadmium in the form of cadmium chloride and sprayed with 0.01 microM of 28-homobrassinolide (HBL) at 30-day stage. The data indicated that plant fresh and dry mass, number of nodules, their fresh and dry mass, leghemoglobin content, nitrogen and carbohydrate content in the nodules, leaf chlorophyll content, nitrate reductase and carbonic anhydrase activities decreased proportionately with the increasing concentrations of cadmium but the content of proline and the activities of catalase, peroxidase and superoxide dismutase increased. The ill effect, generated by cadmium, was overcome if the stressed plants were sprayed with HBL.