Massive cyanobacterial water blooms and production of toxins (cyanotoxins) have become a worldwide problem. In this report,
we present results of cyanotoxins analyses (peptide microcystins, alkaloid cylindrospermopsin) in the Czech Republic reservoirs
using HPLC-PDA and ELISA. Our study suggests the occurrence of cylindrospermopsin in the Czech Republic for the first time
(particularly, in water blooms containing Aphanizomenon klebahnii). We also discuss human health risks associated with microcystins in relation to the drinking water guideline value of 1.0 μg/l
as recommended by the World Health Organization. 相似文献
To assess the interaction between testosterone (T) treatment and acclimation to different salinities, seawater-acclimated
gilthead sea bream (Sparus auratus) were implanted with slow-release coconut oil implants alone (control) or containing T (5 μg/g body mass). After 5 days,
eight fish of control and T-treated groups were sampled. The same day, eight fish of each group were transferred to low salinity
water (LSW, 6 ppt, hypoosmotic test), seawater (SW, 38 ppt, control test) and high salinity water (HSW, 55 ppt, hyperosmotic
test) and sampled 9 days later. Gill Na+, K+-ATPase activity increased in HSW-acclimated fish with respect to SW- and LSW-acclimated fish in both control and T-treated
groups. Kidney Na+, K+-ATPase activity was also enhanced in HSW-acclimated fish, but only in T-treated group. From a metabolic point of view, most
of the changes observed can be attributed to the action of salinity and T treatment alone, since few interactions between
T treatment and osmotic acclimation to different salinities were observed. Those interactions included in treated fish: in
the liver, decreased capacity in using glucose in fish acclimated to extreme salinities; in the gills, decreased capacity
in using amino acids in HSW; in the kidneys increased capacity in using amino acids in extreme salinities; and in the brain,
decreased glycogen and acetoacetate levels of fish in LSW. 相似文献
Environmental Science and Pollution Research - Batch experiments were used to test P sorbent potential of soil samples, pyritic and granitic materials, mussel shell, mussel shell ash, sawdust, and... 相似文献
This research aims to compare the disinfection and degradation effectiveness in water of a commercial suspension of nano-TiO2 (TiO2Levenger) with the standard TiO2Degussa P25. Photo-inactivation and photo-degradation experiments were conducted with UVA-vis light. Concerning the disinfection, the effects of TiO2 dose (0–2 g/l), water matrix, bacterium type (Gram-positive or Gram-negative), and bacterial regrowth after the photo-treatments were studied for each catalyst. The experimental results show that Enterococcus sp. (Gram-positive) was more resistant to the photo-treatments than Escherichia coli (Gram-negative) for both catalyst; however, postirradiation trends showed similar behavior for both bacteria, favoring regrowth for short-treated cells and decay for longer-treated ones. Caffeine was selected as a model substance of pharmaceuticals and personal care products. In terms of caffeine removal, the effects of TiO2 dose (0–2 g/l) and water matrix were analyzed. Besides, the comparison between mechanical coagulation-flocculation-decantation and simple decantation of TiO2 was carried out. The results show that simple decantation allowed the recovery of 97.5% of TiO2 Degussa P25 and TiO2 Levenger within 1 day of simple decantation, while applying the proposed mechanical coagulation-flocculation decantation 99.7% of recovery of both catalysts was achieved in 2 hours. Finally, the subsequent reuse of both catalysts was proved with little loss of efficiency in terms of photo-disinfection during the four cycles. Nevertheless, the standard TiO2 Degussa P25 photo-degradation efficiency of caffeine decreases considerably as compared to commercial suspension of TiO2 Levenger concerning the reutilization.
Chitosan (CHS), chitosan–Eudragit® EPO (CHS–EPO) and chitosan beads partially cross-linked with glutaraldehyde (CHS–GLUT) were obtained in order to improve the adsorption selectivity of endoglucanase from a fungal culture obtained under SSF by Aspergillus niger using soybean harvest residues as support. The matrices synthetized were characterized in terms of physical and chemical changes. Fourier-transformed infrared spectroscopy with attenuated total reflectance device (FTIR-ATR) was employed to verify the chemical changes on the CHS matrix after the synthesis of CHS–GLUT and CHS–EPO. Scanning electron microscopy (SEM) was performed to compare the surface morphology of the polymeric beads. Two variables, purification factor and yield percentage of the adsorption process, were analyzed using a bifactorial ANOVA considering the matrix–time first order interaction. SEM results exhibited greater surface roughness in the CHS–GLUT and CHS–EPO matrices which may enhance endoglucanase adsorption. FTIR-ATR results confirmed an effective chemical modification of the CHS matrix after crosslinking with GLUT and corroborated the efficiency of the synthesis of the CHS–EPO matrix by the presence of chemical groups of the EPO polymer. An endoglucanase purification factor close to 9 was achieved with the CHS–GLUT matrix and a yield percentage of 60% was obtained with the CHS–EPO matrix. Bifactorial ANOVA results showed the matrix–time interaction to be significant for both variables. The CHS–GLUT matrix with low crosslinking times and the novel CHS–EPO matrix could be included in the bioseparation stage of endoglucanase using a simple and a low-cost method such as batch adsorption. 相似文献
Environmental Science and Pollution Research - Lignin is one of the main barriers to obtaining added-value products from cellulosic fraction of lignocellulosic biomass due to its random aromatic... 相似文献
Environmental Science and Pollution Research - The cytotoxicity of nanoparticles (NPs) and their properties are important issues in nanotechnology research. Particularly, NPs affect the metabolism... 相似文献
Plastics from cellular phones, an important contribution to electronic waste, have been studied for catalytic recycling to liquid fuels through hydrocracking. High impact polystyrene and polybutadiene, a component of high impact polystyrene and acrylonitrile–butadiene–styrene terpolymer, majority components in these residues according to literature, have been also studied in the process, using a bifunctional Pt/Hβ catalyst and under kinetic control. The results show a high yield to liquid fuels in the gasoline range with high impact polystyrene and polybutadiene, with a high content of naphthenics and isoparaffins in the catalytic process. Plastics from cellular phones, with a complex composition, require a previous removal of silicone, to prevent to formation of solids. Once silicone is removed, these residues, with a majority yield to liquids, could be treated together with other plastic wastes in catalytic hydrocracking. 相似文献
Current observed as well as projected changes in biodiversity are the result of multiple interacting factors, with land use and climate change often marked as most important drivers. We aimed to disentangle the separate impacts of these two for sets of vascular plant, bird, butterfly and dragonfly species listed as characteristic for European dry grasslands and wetlands, two habitats of high and threatened biodiversity. We combined articulations of the four frequently used SRES climate scenarios and associated land use change projections for 2030, and assessed their impact on population trends in species (i.e. whether they would probably be declining, stable or increasing). We used the BIOSCORE database tool, which allows assessment of the effects of a range of environmental pressures including climate change as well as land use change. We updated the species lists included in this tool for our two habitat types. We projected species change for two spatial scales: the EU27 covering most of Europe, and the more restricted biogeographic region of ‘Continental Europe’. Other environmental pressures modelled for the four scenarios than land use and climate change generally did not explain a significant part of the variance in species richness change. Changes in characteristic bird and dragonfly species were least pronounced. Land use change was the most important driver for vascular plants in both habitats and spatial scales, leading to a decline in 50–100% of the species included, whereas climate change was more important for wetland dragonflies and birds (40–50 %). Patterns of species decline were similar in continental Europe and the EU27 for wetlands but differed for dry grasslands, where a substantially lower proportion of butterflies and birds declined in continental Europe, and 50 % of bird species increased, probably linked to a projected increase in semi-natural vegetation. In line with the literature using climate envelope models, we found little divergence among the four scenarios. Our findings suggest targeted policies depending on habitat and species group. These are, for dry grasslands, to reduce land use change or its effects and to enhance connectivity, and for wetlands to mitigate climate change effects. 相似文献
Bioaccumulative metals such as mercury are found in increasing amounts in fish and their consumers. In the region of the Madeira River, in the Brazilian Amazon, mercury (Hg) is a predominant contaminant in the aquatic ecosystem. There is therefore a need to find specific biomarkers of mercury toxicity in fish to monitor contaminations. Here, mercury-bound proteins were identified in the liver tissues of fishes Mylossoma duriventre and Brachyplatystoma rousseauxii. Mercury was quantified in liver tissue, pellets and protein spots by graphite furnace atomic absorption. Proteins were fractionated by two-dimensional polyacrylamide gel electrophoresis and identified by mass spectrometry with electrospray ionization. We identified nine proteins linked to mercury and that presented biomarker characteristics of mercury. Among the proteins identified, isoforms of parvalbumin, ubiquitin-40S ribosomal protein S27a, brain-specific angiogenesis inhibitor 1-associated protein 2-like protein 2 and betaine–homocysteine S-methyltransferase 1 are notable for having the molecular function of binding to metallic ions. 相似文献